37 results about "Transcription Repressor" patented technology

A product comprising two or more artificial transcription repressors (ATRs), or polynucleotides encoding therefor, selected from groups (a), (b), (c) or (d): (a) an ATR comprising a DNA-binding domain operably linked to a KRAB domain or homologue thereof; (b) an ATR comprising a DNA-binding domain operably linked to a DNMT3A, DNMT3B or DNMT1 domain or homologue thereof; (c) an ATR comprising a DNA-binding domain operably linked to a DNMT3L domain or homologue thereof; and (d) an ATR comprising a DNA-binding domain operably linked to a SETDB1 domain or homologue thereof, wherein at least two of the ATRs are selected from different groups (a), (b), (c) or (d).

By inhibiting the function of a transcription factor that promotes transcription of a gene associated with the amounts of lignin and cellulose, a plant in which the amounts of lignin and cellulose are reduced without reducing the amount of glucan is produced. In this plant, glucan in the obtained cell wall components is in the state of highly easily undergoing saccharification. In this plant, moreover, natural dehiscence of pods is suppressed. A method of inhibiting the transcription factor includes a method in which a chimeric gene between a transcription factor gene and a polynucleotide that encodes a functional peptide capable of converting the transcription factor into a transcription repressor is introduced into a plant cell so that a chimeric protein in which the transcription factor is fused with the functional peptide is produced in a plant cell, and a method of inhibiting the expression of the transcription factor, such as knockout method or RNAi method. Thus, a plant in which the amounts of lignin and cellulose are reduced without reducing the amount of glucan is provided.

Targeted transcriptional effectors (transcription activators and transcription repressors) derived from meganucleases are described. Also described are nucleic acids encoding same, and methods of using same to regulate gene expression. The targeted transcriptional effectors can comprise (i) a meganuclease DNA-binding domain lacking endonuclease cleavage activity that binds to a target recognition site; and (ii) a transcription effector domain.

The invention discloses a gene expression regulation method and a gene expression regulation system based on Type I‑F CRISPR / Cas. The present invention shows for the first time that the Cascade complex belonging to the Type I-F CRISPR / Cas system, such as the PaeCascade complex, can efficiently bind to the target site in mammalian cells; at the same time, the present invention conducts the Type I-F CRISPR / Cas system The mammalian expression system has been optimized so that it can efficiently recruit transcriptional activators or transcriptional repressors to target sites in mammalian cells, and successfully constructed a transcriptional activation system and a transcriptional repression system. The invention makes it possible to apply the Type I-F CRISPR / Cas system in the gene regulation of mammalian cells, and provides a necessary tool for gene transcription regulation based on the Type I-F CRISPR / Cas system.

The invention discloses a GntR family transcription inhibitor mutant, a mutant gene and an application thereof in preparing vitamin B2. Wherein the amino acid sequence of the mutant has the following mutations relative to the sequence shown in SEQ ID No.3: the 89th amino acid is replaced by M. The 89th amino acid coding nucleotide in the GntR family transcription repressor gene on the Bacillus subtilis chromosome is subjected to site-directed mutation to encode the amino acid M, and the genetic engineering bacteria obtained have a relatively improved ability to produce vitamin B2 , and is beneficial to the growth of bacteria, so it has great application and promotion value.

Targeted transcriptional effectors (transcription activators and transcription repressors) derived from meganucleases are described. Also described are nucleic acids encoding same, and methods of using same to regulate gene expression. The targeted transcriptional effectors can comprise (i) a meganuclease DNA-binding domain lacking endonuclease cleavage activity that binds to a target recognition site; and (ii) a transcription effector domain.

The invention discloses a ribonucleotide reductase transcription inhibitor mutant, a mutant gene and its use in the preparation of vitamin B 2 in the application. Wherein the amino acid sequence of the mutant has the following mutation relative to the sequence shown in SEQ ID No.3: the 26th amino acid is replaced by H. The genetically engineered bacteria obtained by replacing the 26th amino acid encoded by the ribonucleotide reductase transcription inhibitor gene on the chromosome of Bacillus subtilis with H by site-directed mutation, which can produce vitamin B 2 The ability has been greatly improved, and it has great application and promotion value.

The invention relates to a MYB gene for regulating the elongation and growth of cotton fibers and its application. The gene is derived from Gossypium hirsutum, and its full-length nucleotide sequence is 1149 bp, without introns, and its open reading frame is 933 bp, encoding a protein with 310 amino acids. This transcription factor has a typical MYB domain and a transcriptional repressor-binding domain (EAR-domain). The present invention verifies the function of the gene in regulating the elongation and development of cotton fibers through genetic transformation. Using RNAi technology to reduce the expression of the gene in cotton fibers can significantly reduce the length of cotton fibers, indicating that the gene is a positive regulator of cotton fiber elongation. The long-developed genes are of great value in the improvement of cotton fiber quality, and can be used for molecular breeding research on cotton fiber quality improvement through hybridization, transgenic and other methods.

The invention relates to the field of agricultural biotechnology, in particular to Trichoderma reesei cellulase transcription inhibitor 70351 application and method of improving cellulase expression and enzymatic activity. The cellulase expression-related transcriptional repressor provided by the present invention 70351 It has a regulatory effect on the expression of cellulase activity. By constructing a knockout plasmid of this gene and transforming it into the host bacteria, the transcriptional repressor is knocked out in the host bacteria. 70351 The expression can improve the protein expression and cellulase activity of the host bacteria. The invention enriches the transcriptional regulation network of Trichoderma reesei cellulase, and provides a new approach for improving the yield of cellulase, reducing the cost of cellulase and realizing effective utilization of cellulose.

The invention belongs to the technical field of tobacco genetic engineering, and specifically relates to a tobacco transcription repressor protein OFP1 and a patent application for its application. Tobacco transcription repressor protein OFP1 consists of 87 amino acid residues, of which the 34‑83 amino acid conserved domain. This protein is related to the content of pigment substances in plant leaves. After reducing the expression of this protein, the content of pigment substances in leaves is significantly reduced. The pigment substances are: neoxanthin, violaxanthin, lutein, chlorophyll a / b ,β-carotene. Through preliminary research on the tobacco transcription repressor protein OFP1 gene, the present invention finds that it is highly correlated with the content of pigment substances in tobacco, and after it is silenced, the content of pigment substances in tobacco is significantly reduced. Utilizing this characteristic can provide new reference and reference for the cultivation of new varieties of tobacco plants.