37 results about "Transcription Repressor" patented technology

Transcription of a gene associated with formation of floral organs is suppressed to produce a sterile plant. A plant cell is transfected with a chimeric gene that includes (i) a coding gene of a transcription factor that promotes expression of a gene associated with formation of floral organs, and (ii) a polynucleotide that encodes a functional peptide that converts an arbitrary transcription factor into a transcription repressor, and a chimeric protein in which the transcription factor is fused with the functional peptide is expressed in the plant cell. The expression of the gene associated with formation of floral organs is dominantly suppressed by the chimeric protein, and as a result a male sterile plant is produced that cannot properly form pollen. The chimeric protein also suppresses expression of a gene associated with dehiscence of anther, and as a result a plant is produced in which dehiscence of anther is suppressed. Further, the chimeric protein suppresses expression of target genes of a transcription factor associated with formation of stamen and pistil, and as a result a double flowered plant is produced.

Transcription of a gene associated with formation of floral organs is suppressed to produce a sterile plant. A plant cell is transfected with a chimeric gene that includes (i) a coding gene of a transcription factor that promotes expression of a gene associated with formation of floral organs, and (ii) a polynucleotide that encodes a functional peptide that converts an arbitrary transcription factor into a transcription repressor, and a chimeric protein in which the transcription factor is fused with the functional peptide is expressed in the plant cell. The expression of the gene associated with formation of floral organs is dominantly suppressed by the chimeric protein, and as a result a male sterile plant is produced that cannot properly form pollen. The chimeric protein also suppresses expression of a gene associated with dehiscence of anther, and as a result a plant is produced in which dehiscence of anther is suppressed. Further, the chimeric protein suppresses expression of target genes of a transcription factor associated with formation of stamen and pistil, and as a result a double flowered plant is produced.

The invention discloses ingenol and application of a derivative of ingenol in enhancement of generation of lysosome. The application specifically comprises optional application of HEP14 or the derivative thereof in the following applications: induction of generation of the lysosome; preparation of drugs for induction of generation of the lysosome; and preparation of drugs for treating and/or preventing lysosome functional disturbance related diseases. HEP14 can activate PKC alpha and PKC delta, on one hand, transcription factors TFEB are activated through two parallel signal pathways, and on the other hand, transcription inhibitors are inactivated and are finally used as 'molecular switches' to control generation of the lysosome. Activation of PKC causes inactivation of GSK3 beta, then transfer of TFEB dephosphorylation to cell nucleus is caused, meanwhile, PKC which is in an activated state can further activate MAPK kinase JNK2 and p38 and phosphorylate ZKSCAN3, and ZKSCAN3 can be transferred to cytoplasm from cell nucleus. HEP14 does not affect activity of mTORC1, therefore, cell metabolism balance is not disturbed, and the ingenol is a perfect drug for treating lysosome related diseases possibly.

The invention discloses a gene expression regulation and control method and system based on Type I-F CRISPR/ Cas. The first study shows that a Cascade compound, such as a PaeCascade compound, belonging to a Type I-F CRISPR/ Cas system can be efficiently combined with a target site in a mammalian cell; and meanwhile, the Type I-F CRISPR/ Cas system is subjected to mammalian expression system optimization, so that a transcription activation factor or a transcription inhibition factor can be efficiently recruited to the target site in the mammalian cell, and a transcription activation system anda transcription inhibition system are successfully constructed. The application of the Type I-F CRISPR/ Cas system in the aspect of gene regulation and control of the mammalian cell becomes possible,and a necessary tool is provided for gene transcription regulation and control based on the Type I-F CRISPR/ Cas system.

The invention discloses an MYB transcription inhibition factor LrMYB3 related to lycium ruthenicum anthocyanin synthesis and application thereof, and belongs to the technical field of gene engineering. According to transcriptome data of lycium ruthenicum, the MYB transcription inhibition factor LrMYB3 participating in anthocyanin synthesis is screened and cloned according to annotation results and expression quantity differences of MYB transcription factors, and belongs to a R2R3 type MYB transcription factor. Multiple sequence alignment and evolutionary tree analysis show that the transcription inhibition factor belongs to a FaMYB1-like transcription inhibition factor. QRT-PCR analysis shows that: the LrMYB3 is expressed in each tissue of the lycium ruthenicum, and the expression level is gradually increased along with ripening of the lycium ruthenicum. Subcellular localization and transcriptional activity detection experiments show that the LrMYB3 is a transcription factor which is localized in a cell nucleus and has no activation function.

The invention discloses a GntR family transcription inhibitor mutant, a mutant gene and an application of the mutant gene to preparation of vitamin B2. The amino acid sequence of the mutant has the following mutation relative to the sequence shown in SEQ ID No.3: the 89th amino acid is replaced with M. The 89th amino acid encoding nucleotide in the GntR family transcription inhibitor gene on the chromosome of bacillus subtilis is subjected to site-specific mutagenesis to encode the amino acid M. The vitamin B2 production capacity of the obtained genetically engineered bacterium is greatly improved, and growth of the bacterium is facilitated, so that high application and popularization values are achieved.

The invention belongs to the technical field of genetic engineering and microorganism, and particularly relates to a preparation method and application of bacilli for efficiently metabolizing glycerol. According to the method, a molecular biology technology is used; a transcription inhibition factor gene ccpC is knocked out in bacillus licheniformis to obtain a bacillus licheniformis engineering strain WX-02 delta ccpC with the ccpC being deleted, and the glycerol metabolizing speed of the bacillus licheniformis is obviously improved. The glycerol consumption speed of the WX-02 delta ccpC in different culture mediums is at least improved by 18.38 percent through being compared with that of an original bacterium of the bacillus licheniformis; the improvement value can reach 32.67 percent tothe highest degree. When the strain is used in a poly gamma-glutamic acid fermentation culture medium, the yield of the poly gamma-glutamic acid can be obviously improved and is at least improved by10.7 percent; the improvement value can reach 17.4 percent to the highest degree. The result shows that the genetic engineering transformation method has important effect on improving the glycerol metabolizing effect; the efficiency of synthesizing bio-based chemicals by the glycerol is improved.

The invention discloses an MYB transcription inhibition factor LrETC1 related to lycium ruthenicum anthocyanin synthesis and application thereof, and belongs to the technical field of gene engineering. According to the invention, the MYB transcription inhibition factor LrETC1 participating in the anthocyanin synthesis is screened and cloned, the total cDNA of the gene is 240bp, and 79 amino acids are encoded; protein sequence database analysis shows that the transcription factor belongs to R3 type MYB; and multiple sequence alignment and evolutionary tree analysis show that the transcription inhibition factor belongs to AtCPC-like transcription inhibition factors, and is a transcription factor which is located in a cell nucleus and does not have an activation function. A LrETC1 transgenic arabidopsis thaliana strain is obtained through agrobacterium tumefaciens-mediated genetic transformation, compared with a wild type, seed coats of seeds are light brown, arabidopsis thaliana seedlings are free of pigment accumulation under the stress condition of high sucrose concentration, and the expression level of related structural genes is remarkably reduced.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a function and application of a transcription inhibitor LIP1 for regulating and controlling rice yield. A cloned LIP1 protein gene interacts with a LAX1 gene, and is expressed in the later developing stage of the meristem of a lateral spikelet and inhibits the transcriptional activity of the LAX1 protein by interaction with the LAX1. The deletion or space-time ectopic expression of the LIP1 gene functions can change the space-time activity of the LAX1 protein and influence formation of rice spikelet. The spatio-temporal expression of the LIP1 defines the activation range of LAX1 in spikelet lateral meristem, and ensures formation of rice lateral spikelet so as to control the yield. By utilizing the cloned LIP1 gene and the encoding protein thereof, the space-time activity of LAX1 can be changed by regulating and controlling the expression activity of the LIP1 gene through a gene manipulation means, so that the formation of spikelet is regulated and controlled, and important significance is achieved on genetic improvement of rice yield traits.

The invention relates to the technical field of agricultural biology, in particular to application of a Trichoderma reesei cellulase transcription inhibition factor 70351 and a method for improving cellulase expression quantity and enzyme activity. The transcriptional inhibition factor 70351 related to cellulase expression provided by the invention has a regulation effect on cellulase activity expression, a knockout plasmid of the gene is constructed and is used for converting host bacteria, and the expression of the transcriptional inhibition factor 70351 is knocked out from the host bacteria, so that the protein expression quantity of the host bacteria and the cellulase activity can be improved. The invention enriches the transcriptional regulation network of the trichoderma reesei cellulase, and provides a new way for improving the yield of the cellulase, reducing the cost of the cellulase and realizing the effective utilization of the cellulose.

The invention belongs to the technical field of tobacco genetic engineering, and particularly relates to a tobacco transcription repressor protein OFP1 and application thereof. The tobacco transcription repressor protein OFP1 is composed of 87 amino acid residues, wherein the amino acids from 34 locus to 83 <rd> locus are at a conserved structure domain. The protein is relevant to the contentof pigment substances in plant leaves, and after the expression of the protein is lowered, the content of the pigment substances in the leaves is significantly lowered, wherein the pigment substancesare neoxanthin, violaxanthin, xanthophyll, chlorophyll a/b and beta-carotene. According to the tobacco transcription repressor protein OFP1 and the application thereof, through the preliminary studyon genes of the tobacco transcription repressor protein OFP1, it is found that the genes are highly relevant to the content of pigment substances in tobacco, and after the genes are silenced, the content of the pigment substances in the tobacco is significantly lowered. By utilizing the feature, a new reference can be provided for cultivation of new varieties of tobacco plants.