25 results about "Transcription Repressor" patented technology

The invention belongs to the field of biosynthesis, and in particular relates to LBD (Levandanenediol Pyrophosphate) synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, the sclareol biosynthesis yield is generally low, LSLPPs mutant containing at least one mutation of D730L, D374L, N674L or G379M is obtained by performing mutation and optimization on LSLPPs pyrophosphate synthase, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, and the yield of a 5L fermentation tank reaches 26.11 g / L and is greatly improved compared with the prior art.

The invention belongs to the field of biosynthesis, and particularly relates to sclareol synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, sclareol biosynthesis yield is generally low, sclareol synthase is mutated and optimized, an SsScs mutant containing at least one mutation of Y491F, G307L or N269F is obtained, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, and the yield of a 5L fermentation tank reaches 21.13 g / L and is greatly improved compared with the prior art.

The invention belongs to the technical field of gene engineering, and particularly relates to application of corn zma-miR319 and / or a target gene of the corn zma-miR319 in regulation and control of corn stem rot resistance. According to the application, sequencing data of small RNA, a degradation group and a transcriptome are integrated, a miRNA family member zma-miR319 is identified, and the resistance of corn to stem rot can be positively regulated and enhanced; the target gene ZmMYB74 is a core regulation gene of an Fg response module, the stem rot resistance of a transgenic plant over-expressing the ZmMYB74 is weakened, and lignin deposition is reduced; the resistance is obviously enhanced by knocking out or inhibiting ZmMYB74, the ZmMYB74 is used as a transcription inhibition factor, the expression of a lignin synthesis related gene ZmCAD is negatively regulated and controlled, and the separation of the disease-resistant gene ZmMYB74 not only contributes to the cultivation of a persistent disease-resistant corn variety, but also deepens the understanding of a stem rot resistance molecular mechanism.

Method of increasing protein content in a eukaryotic cell comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site; method of producing a plant with increased protein content comprising crossing and selecting for increased protein content; method of increasing resistance to a pathogen or a pest in a plant cell or plant comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site, alone or in further combination with expressing QQS in the plant cell or plant; method for producing a plant with increased resistance to a pathogen or a pest comprising crossing and selecting for increased resistance to the pathogen or the pest; a cell, collection of cells, tissue, organ, or organism, such as a plant, in which the NF-YC4 gene comprises a promoter comprising a transcriptional repressor binding site that has been modified so that the transcriptional repressor cannot prevent transcription of the NF-YC4; plants and hybrids thereof; and seeds.

This invention belongs to the field of plant molecular biology and genetic breeding technology, specifically relating to a molecular marker related to the malic acid content of apple fruit and its application. The molecular marker is... MdNAC029 The single nucleotide polymorphism site in the gene promoter region, this site is located MdNAC029 The nucleotide sequence of the gene promoter region, starting from the 2693rd base at the 5' end, corresponds to... MdNAC029 At a position 442 bp upstream of the transcription start codon, the polymorphism is either T or C, with the C / C genotype indicating high malic acid content and the T / T genotype indicating low malic acid content. This invention, through molecular biology experiments, confirms that the T allele is the binding site for the transcriptional repressor MdMYC2, and the C allele eliminates the binding of MdMYC2, thereby relieving transcriptional repression and promoting malic acid accumulation. This provides a new molecular marker for the genetic improvement of apple fruit acidity traits, accelerating the breeding process of superior varieties.

Method of increasing protein content in a eukaryotic cell comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site; method of producing a plant with increased protein content comprising crossing and selecting for increased protein content; method of increasing resistance to a pathogen or a pest in a plant cell or plant comprising an NF-YC4 gene comprising modifying the transcriptional repressor binding site, alone or in further combination with expressing QQS in the plant cell or plant; method for producing a plant with increased resistance to a pathogen or a pest comprising crossing and selecting for increased resistance to the pathogen or the pest; a cell, collection of cells, tissue, organ, or organism, such as a plant, in which the NF-YC4 gene comprises a promoter comprising a transcriptional repressor binding site that has been modified so that the transcriptional repressor cannot prevent transcription of the NF-YC4; plants and hybrids thereof, and seeds.

The invention discloses a method for enhancing cholesterol uptake of saccharomyces cerevisiae, and belongs to the technical field of bioengineering. The method comprises the following steps: knocking out transcription inhibition factors MOT3 and ROX1 of genes related to cholesterol uptake and transport of saccharomyces cerevisiae; the transcription activating factors SUT1 and UPC2-1 of the cholesterol transporter gene are over-expressed; expression of sterol esterifying enzyme ARE2 is enhanced, so that uptake and storage of exogenous cholesterol by the saccharomyces cerevisiae are further enhanced. According to the method disclosed by the invention, the uptake and storage of cholesterol by the saccharomyces cerevisiae are enhanced, and an important reference is provided for construction of a steroid hormone synthesis chassis taking cholesterol as a substrate.

This invention belongs to the field of biosynthesis, specifically relating to a variant of lysine pyrophosphate diol synthase and the constructed engineered yeast strain for perillaldehyde synthesis. To address the generally low yield of perillaldehyde biosynthesis in existing technologies, this invention mutates and optimizes the lysine pyrophosphate diol synthase to obtain an SsLPPs mutant containing the G379M mutation, which is then used for perillaldehyde synthesis. Simultaneously, to address the genetic instability issues associated with free plasmid expression in some Saccharomyces cerevisiae strains, this invention constructs a perillaldehyde synthesis pathway within the yeast genome. Furthermore, by enhancing the acetyl-CoA synthesis pathway, the MVA pathway, and the perillaldehyde synthesis pathway, and by knocking out some transcriptional repressors, the substrate strain synthesizing perillaldehyde is modified, resulting in a significantly increased yield compared to existing technologies.

The invention discloses application and application of an MSP gene to regulation and control of the number of grains per panicle of rice, the CDS sequence of the MSP gene is shown as SEQ ID No.1, and the amino acid sequence of encoded protein is shown as SEQ ID No.2. The MSP gene serves as a transcription inhibition factor to interact with a co-inhibition factor OsTPR2, specifically binds to a P4 site containing CArG-box of a downstream target gene TAWAWA1 promoter, recruits histone deacetylase to inhibit TAWAWA1 expression, and is used for regulating and controlling the number of grains per panicle of rice. The rice inflorescence morphology is regulated and controlled, and the grain number per ear is regulated and controlled. According to the invention, a promoter of a glume protection / glume specific expression gene is fused with an MSP gene CDS to construct a fusion vector, and an msp mutant is transformed, so that an incomplete complementary plant with normal glume protection, recovered grain size and maturing rate and higher grain number per ear than that of a wild type is obtained, and the defects that the grain of the msp mutant becomes small and the like are overcome. The rice inflorescence development regulation theory is expanded, and a theoretical basis and a new thought are provided for high-yield molecular breeding.

The invention belongs to the field of biosynthesis, and in particular relates to an improved variant of libdanenediol pyrophosphate synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, the sclareol biosynthesis yield is generally low, LsLPPs mutant containing N674L mutation is obtained by performing mutation and optimization on LsLPPs pyrophosphate synthase, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; and the chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, so that the yield is greatly improved compared with the prior art.

The present invention aims to treat polyglutamine diseases by silencing the expression of pathogenic alleles using engineered transcription repressors (ETRs). The provided ETRs deposit one or more epigenetic marks that can silence a pathogenic allele.

The problem addressed by the present invention is to provide a pyrrole imidazole polyamide (PIPA) for transcriptionally inhibiting the expression of cytokines necessary for immune response activation, particularly type I IFNs. More specifically, the present invention is a PIPA that specifically binds to a binding region of an IFN regulatory factor (IRF) family transcription factor. Specifically, said PIPA binds to 5'-GAAAGTG-3 (sequence number 1) or a modified sequence thereof, 5'-GAAAG-3' (sequence number 9) or a modified sequence thereof, 5'-GAAAA-3' (sequence number 15) or a modified sequence thereof, or 5'-GAAAC-3' (sequence number 21) or a modified sequence thereof.

The disclosure relates to gene repressor systems comprising catalytically-dead Class 2 CRISPR proteins and one or more transcription repressor domains linked to the catalytically-dead Class 2 CRISPR protein as a fusion protein, as well as a guide ribonucleic acid (gRNA); and methods of making and using same.

The present application belongs to the field of biosynthesis, and particularly relates to a sclareol synthase and a yeast engineering bacterium for producing sclareol. In order to solve the problem of low yield of sclareol biosynthesis in the prior art, the sclareol synthase is mutated and optimized in the present application, a SsScs mutant containing at least one mutation of Y491F, G307L or N269F is obtained, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability existing in the expression of free plasmid in some Saccharomyces cerevisiae strains, the present application constructs a sclareol synthesis pathway in the yeast genome, strengthens the acetyl coenzyme A synthesis pathway, the MVA pathway and the sclareol synthesis pathway, and knocks out part of the transcriptional repressor to modify the chassis bacterium for synthesizing sclareol. The yield in a 5 L fermenter reaches 21.13 g / L g / L, which is greatly improved compared with the prior art.

The invention provides an engineering strain for synthesizing L-methionine by using ethylene glycol and application of the engineering strain. The engineering strain comprises an expression plasmid vector or an expression plasmid vector combination, the vector or combination comprises a first group of polynucleotides and a second group of polynucleotides, and the first group of polynucleotides comprises at least one polynucleotide encoding an enzyme promoting ethylene glycol utilization; the second set of polynucleotides comprising at least one polynucleotide encoding an L-methionine synthesis pathway enzyme; and a backbone plasmid capable of autonomously replicating in a host cell. By introducing the ethylene glycol utilization gene, not only is the utilization rate of glucose improved, but also the yield of L-methionine is improved; meanwhile, by overexpressing or strengthening a plurality of L-methionine biosynthetic pathway related genes from different sources and knocking out or weakening L-methionine degradation pathway or transcription inhibition factor related genes, efficient fermentation production of L-methionine is realized.

This invention discloses a MYB transcriptional repressor, AsMYB054, related to the synthesis of 2-(2-phenylethyl) chromones in Aquilaria sinensis and its applications. Targeting the weak links in the synthesis and regulation of 2-(2-phenylethyl) chromone compounds, this invention clones for the first time the AsMYB054 gene, a transcriptional repressor related to the regulation of 2-(2-phenylethyl) chromone synthesis in Aquilaria sinensis. AsMYB054 is located in the cell nucleus and possesses transcriptional repressive activity, capable of binding to and inhibiting the activity of the promoters of enzyme genes AsPKS02 and AsPKS09, which are related to the biosynthesis of 2-(2-phenylethyl) chromone compounds. Inhibiting the expression of AsMYB054 using gene editing or RNA interference techniques can promote the accumulation of 2-(2-phenylethyl) chromone compounds in Aquilaria sinensis, showing broad application prospects and significant economic value.

The invention discloses application of a GBI gene in regulation and control of rice plant type development. The applicant performs over-expression on the member GBI of the rice class II LBD gene family, and the obtained over-expression transgenic plant shows a semi-dwarf phenotype. Gene basic feature analysis finds that GBI is a transcription inhibition factor positioned in a cell nucleus. According to preliminary analysis of a molecular mechanism, transcription of GBI is inhibited by BR and GA at the same time, and GBI interacts with TUD1 participating in a BR signal transduction pathway and SLR1 participating in a GA signal transduction pathway at the same time. The results show that GBI is combined with GA and BR signal transduction pathways to jointly regulate and control the development of the rice plant height.

The present application belongs to the field of biosynthesis, and particularly relates to pyrophosphate lyddane diol ester synthase and yeast engineering bacteria for producing sclareol. In order to solve the problem of generally low yield of sclareol biosynthesis in the prior art, the pyrophosphate lyddane diol ester synthase is mutated and optimized in the present application, and a SsLPPs mutant containing at least one mutation of D730L, D374L, N674L or G379M is obtained, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability existing in the expression of free plasmid in part of saccharomyces cerevisiae strains, the present application constructs a sclareol synthesis pathway in the yeast genome, and at the same time, the acetyl coenzyme A synthesis pathway, the MVA pathway and the sclareol synthesis pathway are strengthened, and part of the transcriptional repressor is knocked out, so that the bottom bacteria for synthesizing sclareol are modified. The yield in a 5 L fermenter reaches 26.11 g / L, and the yield is greatly improved compared with the prior art.

The present application relates to the field of biotechnology, in particular to a high-yield high-performance cellulase Trichoderma reesei engineering strain and a construction method and application thereof, the Trichoderma reesei engineering strain co-overexpresses beta-glucosidase gene Aabgl1 and expansin gene Trswo1, and knocks out transcriptional repressor gene Trace1, the Trichoderma reesei engineering strain has high cellulase yield, which is 100.15% higher than that of the starting strain Trichoderma reesei Rut C30.The high-performance cellulase produced by the Trichoderma reesei engineering strain has balanced enzyme system composition and excellent hydrolysis performance, when corn straw is hydrolyzed, the glucan conversion rate reaches 94.89%, the hydrolysis performance is equivalent to that of commercial enzyme preparation Cellic CTec2.0, and good industrial development prospect and practical application value are shown.

This invention belongs to the field of biosynthesis, specifically relating to a variant of lysine pyrophosphate diol ester synthase and engineered bacteria for producing perillaldehyde. To address the generally low yield of perillaldehyde biosynthesis in existing technologies, this invention mutates and optimizes lysine pyrophosphate diol ester synthase, obtaining an SsLPPs mutant containing the D374L mutation, and uses this mutant for perillaldehyde synthesis. Simultaneously, to address the genetic instability issues associated with free plasmid expression in some Saccharomyces cerevisiae strains, this invention constructs a perillaldehyde synthesis pathway in the yeast genome. Furthermore, it modifies the substrate bacteria synthesizing perillaldehyde by enhancing the acetyl-CoA synthesis pathway, the MVA pathway, and the perillaldehyde synthesis pathway, and by knocking out some transcriptional repressors, resulting in a significant increase in yield compared to existing technologies.

Disclosed in the present invention are a xylose-inducible transcription factor component and a use thereof. The xylose-inducible transcription factor component comprises: a xylose-inducible transcription factor XlnR derived from a filamentous fungus and a binding consensus sequence of the xylose-inducible transcription factor XlnR. By combining the transcription factor XlnR with a prokaryotic xylose-inducible transcriptional repressor XylR, a dual-regulated inducible system obtained exhibits an extremely low leakage level, but maintains a maximum activation level comparable to that of an XlnR single-regulated xylose-inducible system. The present invention solves the problems of low induction strength and slow response in transcription factor-dependent xylose transcriptional induction systems in the field of synthetic biology.

The problem that this invention aims to solve is to provide a Corynebacterium glutamicum mutant strain with improved L-lysine production ability, and a method for producing L-lysine using the mutant strain. [Solution] The present invention relates to a Corynebacterium glutamicum mutant strain with improved L-lysine production ability and a method for producing L-lysine using the same. The Corynebacterium glutamicum mutant strain can increase the supply of precursors and increase sugar utilization, thereby improving the L-lysine production yield, by increasing or enhancing the expression of genes that encode enolase, or simultaneously decreasing or weakening the expression of gluconate operon transcription repressors.

This invention discloses a sugarcane gene ScJAZ10 The sugarcane gene and its application in regulating plant flowering relate to the field of plant biotechnology. ScJAZ10 The nucleotide sequence of the protein is shown in SEQ ID No. 1, and the amino acid sequence of the protein it encodes is shown in SEQ ID No. 2. This invention reveals for the first time that the ScJAZ10 protein can act as an atypical transcriptional coactivator, physically interacting with the ScNAC23 transcription factor in the cell nucleus to form a protein complex, synergistically activating the downstream core florigen gene. ScFT Gibberellin (GA) can activate transcription, thereby promoting earlier flowering in plants. ScJAZ10 The ScJAZ10 protein is transcribed and expressed, and its flowering-promoting function depends on the accumulation of endogenous GA. This invention breaks through the traditional understanding that JAZ proteins are only transcriptional repressors, providing new key gene targets and molecular regulatory modules for the genetic improvement of flowering time in sugarcane and other plants, and has significant theoretical value and broad application prospects.

The present disclosure relates to method of modulating ERα mRNA expression, modulating the activity of the ESR1 transcriptional repressor (MERARs), and treatment of cancers including breast cancer, estrogen receptor positive or "ER(+)" breast cancer, resistant breast cancer, breast cancer with ESR1 mutations, ER positive breast cancer with ESR1 mutations, ER positive resistant breast cancer with ESR1 mutations, prostate cancer, non-small cell lung cancer, and metastatic cancer using MERAR compounds and pharmaceutical compositions comprising the MERAR compounds. A kit comprising one or more of the MERAR compound is also provided.