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42results about How to "Increase consumption rate" patented technology
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High-strength coral aggregate concrete and preparation method thereof
ActiveCN108218350AReduce hydration temperature riseReduce or avoid temperature cracksMetakaolinSeawater
The invention discloses high-strength coral aggregate concrete which is prepared from the following ingredients in parts by weight: 260-350 parts of low-heat marine Portland cement, 35-50 parts of flyash floating beads, 35-50 parts of mineral powder, 8-16 parts of silica fume, 8-16 parts of metakaolin, 2-6 parts of nano silicon dioxide, 2-6 parts of polyaluminum chloride, 550-679 parts of coral reef sand, 890-1000 parts of coral reef and 180-256 parts of seawater. The high-strength coral aggregate concrete has the advantages that the pelagic engineering construction cost is lowered, and environment-friendly and energy-saving requirements of civil engineering are also met.
Owner:CCCC SECOND HARBOR ENG +2
Air inlet connecting pipe of EGR engine
InactiveCN103397959AImprove mixing uniformityIncrease consumption rateNon-fuel substance addition to fuelInternal combustion piston enginesElectronic throttleEngineering
The invention discloses an air inlet connecting pipe of an EGR engine. The air inlet connecting pipe comprises a fresh air inlet single pipe and an exhaust gas mixing tube. The two ends of the fresh air inlet single pipe are provided with a sharing air inlet and a sharing air outlet respectively. The sharing air inlet is connected with a device used for providing fresh air, the sharing air outlet is connected with an air inlet pipe, and an electronic throttle valve which is in control connection with an engine ECU is connected between the sharing air inlet and the sharing air outlet in series. The exhaust gas mixing tube uses the sharing air inlet together with the fresh air inlet single pipe, and an air outlet of the exhaust gas mixing tube is connected into the fresh air inlet single pipe on the upstream portion of the sharing air outlet. A Venturi tube is additionally arranged in the exhaust gas mixing tube, an exhaust gas channel hole which is led to the inside of the Venturi tube is formed in the throat portion of the equal diameter of the Venturi tube, and an exhaust gas air inlet is formed in the side wall of the exhaust gas mixing tube corresponding to the exhaust gas channel hole. According to the air inlet connecting pipe of the EGR engine, a double-channel air inlet mode is adopted, the mixing uniformity of exhaust gas and the fresh air is improved, and nitric oxide in the exhaust gas can be effectively lowered.
Owner:GUANGXI YUCHAI MASCH CO LTD
Method for culturing chlorella by heterotrophism with sludge hydrolysate
InactiveCN102746992ARealize resource utilizationReduce manufacturing costSludge treatment by oxidationUnicellular algaeBiodieselSludge
The invention relates to a method for culturing chlorella by heterotrophism with sludge hydrolysate, relating to a method for culturing the chlorella through heterotrophism. The invention aims at solving the problem that the existing microalgae biodiesel is high in cost. The method disclosed by the invention comprises the following steps of: firstly, carrying out ultrasonic processing on excess sludge of a domestic sewage treatment plant; secondly, carrying out anaerobic hydrolysis on the sludge for 2-3 days under the conditions that rotating speed is 180r / min and temperature is 35 DEG C, regulating the pH value to 9.0, and carrying out nitrogen stripping every 12 hours, so as to obtain the sludge hydrolysate, adding trace elements except nitrogen and phosphorus and other nutrition elements, and carrying out high pressure steam sterilization, so as to obtain a culture solution; and thirdly, inoculating the chlorella in percentage by volume of 10-20%, and then culturing for 150-180 hours in a shaking culture tank, thus the culture of the chlorella is completed. By adopting the method disclosed by the invention, the cost for producing the chlorella can be saved, and low-cost raw materials are provided for commercial production of biodiesel. The method disclosed by the invention is applied to the biodiesel preparation field.
Owner:HARBIN INST OF TECH
Industrial Saccharomyces cerevisiae strain for high-yield production of xylitol and ethanol by co-fermenting xylose and glucose and construction method
InactiveCN108300671AAccurate and fast positioningIncrease consumption rateFungiVectorsConcentrations glucoseXylose
The invention discloses an industrial Saccharomyces cerevisiae strain for high-yield production of xylitol and ethanol by co-fermenting xylose and glucose. The industrial Saccharomyces cerevisiae strain is SEB10 with a preservation number of CGMCC NO. 14777. The invention further relates to a construction method of the Saccharomyces cerevisiae strain, and the method comprises the following steps:selecting an original strain; using a Cre-LoxP system to knock out a KanMX gene of the original strain and construct an SEB6kan strain; mutating an HXT3 gene of the SEB6kan strain by using a CRISPR / Cas9 system to construct an SEB6kan-M-HXT3 strain. Through a CRISPR / CasS9 gene editing technology, the 367th site of a transport protein HXT3 can be rapidly and accurately mutated from asparagine to alanine at a fixed point; under the condition with high initial glucose concentration, the Saccharomyces cerevisiae strain prepared according to the construction method provided by the invention improvesthe xylose transport rate, thereby improving the capability of Saccharomyces cerevisiae to co-ferment xylose and glucose and improving the performance of the Saccharomyces cerevisiae to produce xylitol and ethanol; under the condition with a fermentation tank, the xylose consumption rate is increased by 12%, the xylitol production is increased by 13%, the ethanol production is increased by 22%, and the xylitol yield is close to a theoretical value of 1.0.
Owner:SINOPEC SHANGHAI ENG +2
Method for producing succinic acid
InactiveCN1875108AIncrease consumption rateIncrease spawn rateBacteriaRecombinant DNA-technologyLactate dehydrogenasePyruvate carboxylase
Owner:MITSUBISHI CHEM CORP +1
Method for effectively improving L-malic acid production intensity of Aspergillus oryzae pp25
InactiveCN106222099AIncrease productionIncrease consumption rateFungiMicroorganism based processesBiotechnologyYeast
The invention discloses a method for effectively improving L-malic acid production intensity of Aspergillus oryzae pp25, belonging to the field of genetic engineering. According to the invention, Aspergillus oryzae pp25 is used as a starting strain, a C4-dicarboxylate transport protein gene C4T318 of the Aspergillus oryzae pp25 and a malic acid transport protein gene maeI of Schizosaccharomyces pombe are expressed to obviously reduce the intracellular accumulation of malic acid, and finally, the shake flask yield of the L-malic acid is up to 93.17+ / -2.10g / L and is increased by 47.4%; the concentration of succinic acid is 5.17+ / -0.49g / L and is reduced by 41.8%; and the conversion rate of the malic acid relative to glucose is 0.85g / g and is increased by 49.1%. Meanwhile, the fermentation period is shortened from 90 hours to 80 hours, and the production intensity is up to 1.16g / L.h and is increased by 65.7%.
Owner:JIANGNAN UNIV
Automatic capacity expanding and reducing control method and device, storage medium and processor
ActiveCN110971430AIncrease profitIncrease consumption rateData switching networksMessage queueEngineering
The invention discloses an automatic capacity expanding and reducing control method. The method includes the steps of periodically monitoring the total number of messages in message queue middleware,the message publishing rate of a message producer and the message consumption rate of each message consumer, triggering a preset distributed application capacity expansion mechanism when the current total message number is greater than a preset maximum message number threshold, and triggering a preset distributed application capacity reduction mechanism when the current total message number is less than a preset minimum message number threshold. According to the method, the capacity expansion and reduction control of the distributed application is automatically realized, the state of higher load and the state of lower load of the message queue middleware are monitored in time, the capacity is reasonably expanded when the load is higher, and the capacity is reasonably reduced when the loadis lower, so that the utilization rate of distributed cluster resources is improved.
Owner:BEIJING GRIDSUM TECH CO LTD
Recombinant bacterium with function of pyruvate production intensity improvement and application of recombinant bacterium with function of pyruvate production intensity improvement
ActiveCN105368884AIncrease productionIncrease production intensityFungiMicroorganism based processesTorulopsis glabrataCell membrane
The invention belongs to the technical field of fermentation engineering and discloses a recombinant bacterium with a function of pyruvate production intensity improvement and application of the recombinant bacterium with the function of pyruvate production intensity improvement. By targeting expression of a mitochondrion pyruvate carrier protein to a cell membrane in a torulopsis glabrata strain, cell outgoing rate of pyruvate is increased while a feedback inhibition effect of the pyruvate on glycolytic pathway key enzymes is weakened, and consequently pyruvate yield is increased, and production intensity is improved. By means of genetic engineering technology, a signal peptide sequence Shrew1p and MPC1 derived from saccharomyces cerevisiae are positioned and integrated to a pY26 expression vector to construct a saccharomyces cerevisiae recombinant expression plasmid pY26-Shrew1p-MPC1 which is electrophoretically transferred into a recipient bacterium TgU to obtain an engineering strain TgU-(pY26-Shrew1p-MPC1) high in pyruvate yield. Compared with a reference strain TgU-(pY26), the engineering strain TgU-(pY26-Shrew1p-MPC1) has the advantage that dry cell weight, glucose consumption rate, pyruvate yield and pyruvate production intensity are increased by 58.3%, 68.1%, 154.4% and 152.6% respectively.
Owner:JIANGNAN UNIV
Method for efficiently producing rare earth element reinforced 2-keto-L-gulonic acid
InactiveCN101671715AIncrease consumption rateShorten the fermentation cycleMicroorganism based processesFermentationRare-earth elementGluconic acid
The invention relates to a method for efficiently producing rare earth element reinforced 2-keto-L-gulonic acid, belonging to the technical field of fermentation processes of optimized vitamin C. Themethod utilizes a mixed fungus strain of ketogulonigenium vulgare and bacillus megaterium as a production fungus strain and prompts the production of the 2-keto-L-gulonic acid (2KGA) and the consumption of sorbose by adding cerium ions Ce<3+> or lanthanum ions La<3+> into a culture medium so as to realize the efficient production of the 2KGA. Due to the addition of 5mmol / L of the cerium ions, theoutput of the 2KGA is improved to 72.1g / L from 67.9g / L of a contrast embodiment, the fermentation period is shortened for about 12h, and the average consumption rate of the sorbose and the generationrate of the 2KGA are respectively improved for 23.5 percent and 27.1 percent. Due to the addition of 7mmol / L of the lanthanum ions, the output of the 2KGA is improved to 75.7g / L, the fermentation period is shortened for about 12h, the conversion rate from the sorbose to the 2KGA is improved from 83.1 percent to 91.6 percent, and the average consumption rate of the sorbose and the generation rate of the 2KGA are respectively improved by 22.8 percent and 35.3 percent.
Owner:JIANGNAN UNIV
Device for efficiently and quickly disposing food wastes by using earthworms
InactiveCN110467490AHigh activityAccelerated ripeningBio-organic fraction processingOrganic fertiliser preparationLitterDecomposition
The invention discloses a device for efficiently and quickly disposing kitchen wastes by using earthworms. The device includes a shell, a flip cover, a material storage partition plate, a material guide partition plate, a plurality of stirring screws, a rotating motor, a plurality of material guide rotating rods, an L-shaped crank, a rotating sheet, an LED light strip, a screen layer, a collectionchamber, a material outlet, a square partition plate and a liquid outlet. The device has the advantage of simple structure, convenience in operation, high activity and digestion rate of earthworms, high decomposition rate of the food wastes, ventilating digestion environment of the earthworms, and maximization of the working efficiency of the earthworms by adjusting the temperature and humidity in the device according to the change of the outside environment.
Owner:NINGXIA UNIVERSITY
Method for shortening tobacco leaf baking time of closed barking room
PendingCN107890134AReduce baking timeControl contentTobacco preparationCooking & bakingCellular respiration
The invention discloses a method for shortening the tobacco leaf baking time of a closed barking room, belonging to the technical field of modulation of flue-cured tobacco. The method comprises the following steps: manually introducing oxygen in the front yellowing stage, so as to maintain the oxygen concentration in the closed barking room at 0.5%-2.0%; manually introducing carbon dioxide from the middle-later yellowing stage to a color fixation stage, so as to maintain the carbon dioxide concentration in the closed barking room at 0.5%-2.0%; and continuing to introduce carbon dioxide from alater color fixation stage to a tendon drying stage, so as to maintain the carbon dioxide concentration in the closed barking room at 0.1%-0.8%. The method has the beneficial effects that the respiratory action of tobacco leaves in the baking process can be adjusted, the baking time can be shortened, the quality of the tobacco leave can be improved, the utilization rate of the baking room can be increased, the energy consumption cost can be lowered, and the increase of farmer's income can be promoted.
Owner:云南省烟草公司楚雄州公司 +1
Method for preparing vanillin by virtue of immobilized biological membrane
InactiveCN103981225AImprove toleranceUniform distribution of surface voidsMicroorganism based processesFermentationBiotechnologyHigh concentration
The invention relates to a method for preparing vanillin by virtue of an immobilized biological membrane. The method comprises the following steps: (1) connecting at least one biological membrane reactor filled with fiber carriers with a fermentation tank in series; (2) putting a fermentation medium in the fermentation tank, and then sterilizing the fermentation tank and the biological membrane reactor; (3) inoculating a slant culture of a ring of well-grown bacillus subtilis B7-S ((i)Bacillus subtilis( / i)B7-S) into a shake flask with the fermentation medium for shaking culture, so as to obtain a fermentation liquor; (4) allowing the fermentation liquor to circulate constantly between the biological membrane reactor and the fermentation tank by virtue of a pump, replacing with a fresh fermentation medium till the concentration of the vanillin is not increased, and continuing to ferment to produce the vanillin, wherein the time spent for each circulation of fermentation liquor is not more than 0.5 hour. The method can be used for effectively improving the production efficiency of the vanillin, has the advantages of producing the product with high concentration and being stable in production of each batch, and is suitable for industrial production of the vanillin.
Owner:甘肃省商业科技研究所有限公司
Saccharomyces cerevisiae strain enduring ferulic acid and application of saccharomyces cerevisiae
The invention discloses a saccharomyces cerevisiae strain enduring ferulic acid. The saccharomyces cerevisiae strain is a strain which is obtained after adaptive evolution of saccharomyces cerevisiaewith a ferulic acid inhibitor culture medium and has remarkably improved endurance to ferulic acid, the strain is classified and named as Saccharomyces cerevisiae PAT02 and is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.18022. The saccharomyces cerevisiae strain is capable of enduring ferulic acid, and when the strain is appliedto a fermentation system with the ferulic acid, the growth performance and the ferulic acid degradation velocity of the strain are both higher than those of an original strain.
Owner:HEBEI NORMAL UNIV FOR NATTIES
Data communication method based on MQTT and Websocket
PendingCN114866528AReal-time communication of server resources and bandwidthReduce occupancySecuring communicationData classProxy server
The invention discloses a data communication method based on MQTT and Websocket, and the method comprises the steps: S1, an intelligent terminal device issues an MQTT message, and the MQTT message carries Topic information and also carries data type information subordinate to the Topic information; s2, receiving the MQTT messages by the MQTT message proxy server, and respectively creating message queues for storage according to different Topic of the MQTT messages; s3, the background server subscribes the corresponding Topic to the MQTT message proxy server according to the own demand; and S4, after receiving the corresponding MQTT message, the background server sends the information corresponding to the corresponding data type under the corresponding Topic to the client which establishes long connection with the background server through the Websocket protocol according to the subscription condition of the client for the data type. The invention provides a reliable and timely data communication method for the service scene of intelligent terminal equipment-server-client, and can also reduce the number of Topic in the MQTT message proxy server, thereby reducing the occupation of the disk space of the MQTT message proxy server and improving the message consumption rate in the MQTT message proxy server.
Owner:GUANGDONG MEIWEIXIAN FLAVORING & FOOD
Burner-based GPF (Gasoline Particulate Filter) rapid ash content accumulation aging test device
InactiveCN108489731AImprove atomization effectPromote combustionInternal-combustion engine testingMachine part testingParticulatesOil can
The invention relates to a burner-based GPF (Gasoline Particulate Filter) rapid ash content accumulation aging test device. The device comprises an oil feed unit for providing engine oil and gasoline,a compressed air unit, a co-flow air unit, a dilution air unit, a burner unit and a GPF unit; the burner unit includes a burner cavity, a spark plug disposed on the burner cavity, a combustion disk disposed at the inlet end of the burner cavity, and an air-assisted atomizing nozzle; the oil feed unit, the compressed air unit, and the co-flow air unit are all communicated with the inlet end of theburner cavity through a pipeline; the rear end of the burner cavity is provided with a mixing cavity; the dilution air unit is communicated with the mixing cavity; and the rear end of the mixing cavity is connected with the GPF unit through a gas exhaust pipeline. With the device of the invention adopted, the rapid ash content accumulation aging process of a GPF can be realized, the research anddevelopment costs of an enterprise can be decreased, the largest engine oil-gasoline oil mixture ratio can be selected with the good atomization combustion of mixed fuel oil ensured, and the rate of the consumption of the engine oil can be greatly increased, and test time can be reduce.
Owner:TONGJI UNIV
Energy-saving and environment-friendly medical wastewater treatment device
InactiveCN110759608ASolve quality problemsSolving abilityWater/sewage treatment by irradiationSpecific water treatment objectivesSludgeWater pipe
The invention discloses an energy-saving and environment-friendly medical wastewater treatment device, relates to the technical field of medical wastewater treatment, and aims to solve the problems that treated wastewater is not detected, a water body which is not treated thoroughly cannot be detected, bacteria are not completely eliminated, secondary pollution of the water body is easily caused by discharge, various diseases are caused, and the body health of people is harmed. Supporting columns are arranged below a workbench. A filter box is arranged above the workbench; a water inlet is formed above the filter box; a reaction box is arranged on the other side of the filter box; a dosing port is formed in one side of the motor; an anaerobic tank is arranged on one side of the reaction box, a water outlet pipe is arranged below the anaerobic tank, a disinfection sedimentation tank is arranged on one side of the anaerobic tank, water pipes are arranged at the two ends of a third waterpump, a sludge discharge pipe is arranged below the disinfection sedimentation tank, and a sampling pipe is arranged on one side of the water outlet pipe.
Owner:王华
A strain of acetic acid bacteria and its application in fermenting apple cider vinegar
ActiveCN105018384BStrong ability to produce acetic acidIncrease contentBacteriaMicroorganism based processesMicroorganismApple maggot
The invention discloses an acetic acid bacterium and its application in fermenting apple cider vinegar, belonging to the field of industrial microorganisms. The acetic acid bacterium is a strain of Acetobacter pasteurianus (Acetobacter pasteurianus) CYD159, which was preserved in the China Center for Type Culture Collection on June 30, 2015. The preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2015418. The bacterium has high acid-producing ability, and the acid-producing ability reaches more than 110g / L. The fermented apple cider vinegar produced by using the acetic acid bacterium strain has high acetic acid yield and good flavor, and is an acetic acid bacterium suitable for brewing apple cider vinegar.
Owner:JIANGNAN UNIV
Multi-functional hybrid ultra-filtration membrane as well as preparation method and application thereof
ActiveCN109748360AHigh selectivityImprove removal efficiencyWater contaminantsWater/sewage treatment bu osmosis/dialysisUltrafiltrationMultiple function
The invention discloses a multi-functional hybrid ultra-filtration membrane as well as a preparation method and application thereof, and belongs to the technical field of membrane separation for watertreatment. The ultra-filtration membrane comprises a skin layer and a supporting layer, wherein the skin layer is used for ultra-filtration interception; the supporting layer is used for catalyzing,oxidizing and decomposing organic matters; the skin layer is positioned on the supporting layer; the supporting layer comprises a functional granular material with advanced oxidation catalytic activity. The preparation method of the ultra-filtration membrane comprises the preparation processes: a) dissolving a supporting layer membrane matrix polymer into an organic solvent to prepare a solution A; b) adding the functional granular material with advanced oxidation catalytic activity into the solution A, uniformly mixing, preparing a blended membrane casting solution and preparing the supporting layer by utilizing the blended membrane casting solution; c) membrane-forming a skin layer membrane casting solution on the supporting layer and preparing the skin layer. The ultra-filtration membrane disclosed by the invention not only can improve the advanced oxidation selectivity and improve the removal effect of pollutants, but also can effectively reduce the addition of an oxidant, improvethe stability of the ultra-filtration membrane and prolong the service life.
Owner:NANJING UNIV
Device and method for polycondensation of polyol by using cyclic compound
PendingCN114471403AImprove efficiencyImprove product molecular weight distributionProcess control/regulationChemical/physical/physico-chemical microreactorsCyclic compoundMaterials science
Owner:NANJING YANCHANG REACTION TECH RES INST CO LTD
Method for culturing chlorella by heterotrophism with sludge hydrolysate
InactiveCN102746992BRealize resource utilizationReduce manufacturing costSludge treatment by oxidationUnicellular algaeBiodieselNutrition
The invention relates to a method for culturing chlorella by heterotrophism with sludge hydrolysate, relating to a method for culturing the chlorella through heterotrophism. The invention aims at solving the problem that the existing microalgae biodiesel is high in cost. The method disclosed by the invention comprises the following steps of: firstly, carrying out ultrasonic processing on excess sludge of a domestic sewage treatment plant; secondly, carrying out anaerobic hydrolysis on the sludge for 2-3 days under the conditions that rotating speed is 180r / min and temperature is 35 DEG C, regulating the pH value to 9.0, and carrying out nitrogen stripping every 12 hours, so as to obtain the sludge hydrolysate, adding trace elements except nitrogen and phosphorus and other nutrition elements, and carrying out high pressure steam sterilization, so as to obtain a culture solution; and thirdly, inoculating the chlorella in percentage by volume of 10-20%, and then culturing for 150-180 hours in a shaking culture tank, thus the culture of the chlorella is completed. By adopting the method disclosed by the invention, the cost for producing the chlorella can be saved, and low-cost raw materials are provided for commercial production of biodiesel. The method disclosed by the invention is applied to the biodiesel preparation field.
Owner:HARBIN INST OF TECH
Process for production of succinic acid
InactiveCN101297043AIncrease consumption rateIncrease spawn rateMicroorganism based processesRecombinant DNA-technologyProduct gasSuccinic acid
Owner:AJINOMOTO CO INC
Ice hot body building and weight losing method and equipment suitable for same
InactiveCN102247270ATo promote metabolismImprove perspirationBathing devicesThermal stimulationWhole body
The invention relates to the technical field of body building and weight losing and in particular relates to an ice hot body building and weight losing method and equipment suitable for the same. The ice hot body building and weight losing method comprises the following specific step: simultaneously carrying out thermal stimulation and cold stimulation to two positions of a human body. The equipment suitable for the method comprises a tank body and a tank cover, wherein the lower part of the interior of the tank body is provided with a cold water tank; the upper part of the interior of the tank body is provided with a hot air chamber; and the cold water tank is communicated with the hot air chamber. When the cold water tank carries out cold stimulation on certain position of the human body, vessels of the whole body participate in vasomotor motion to increase the elasticity of the vessels, thereby bringing convenience for preventing and treating arteriosclerosis, hypertension and coronary heart disease, and performing the action on building the body. In addition, the human body is stimulated to quicken metabolism to resist freezing so as to improve the fat consumption rate of the human body; the hot air chamber is used for carrying out thermal stimulation on other positions of the human body; and the thermal environment is utilized to quicken the human body to perspire and quicken the fat to burn so as to finally realize the purpose of losing weight.
Owner:张飞麟
Ice hot body building and weight losing method and equipment suitable for same
InactiveCN102247270BTo promote metabolismImprove perspirationBathing devicesHuman bodyThermal stimulation
The invention relates to the technical field of body building and weight losing and in particular relates to an ice hot body building and weight losing method and equipment suitable for the same. The ice hot body building and weight losing method comprises the following specific step: simultaneously carrying out thermal stimulation and cold stimulation to two positions of a human body. The equipment suitable for the method comprises a tank body and a tank cover, wherein the lower part of the interior of the tank body is provided with a cold water tank; the upper part of the interior of the tank body is provided with a hot air chamber; and the cold water tank is communicated with the hot air chamber. When the cold water tank carries out cold stimulation on certain position of the human body, vessels of the whole body participate in vasomotor motion to increase the elasticity of the vessels, thereby bringing convenience for preventing and treating arteriosclerosis, hypertension and coronary heart disease, and performing the action on building the body. In addition, the human body is stimulated to quicken metabolism to resist freezing so as to improve the fat consumption rate of the human body; the hot air chamber is used for carrying out thermal stimulation on other positions of the human body; and the thermal environment is utilized to quicken the human body to perspire and quicken the fat to burn so as to finally realize the purpose of losing weight.
Owner:张飞麟
A recombinant bacterium with improved pyruvate production intensity and its application
ActiveCN105368884BIncrease productionIncrease production intensityFungiMicroorganism based processesTorulopsis glabrataCell membrane
The invention belongs to the technical field of fermentation engineering and discloses a recombinant bacterium with a function of pyruvate production intensity improvement and application of the recombinant bacterium with the function of pyruvate production intensity improvement. By targeting expression of a mitochondrion pyruvate carrier protein to a cell membrane in a torulopsis glabrata strain, cell outgoing rate of pyruvate is increased while a feedback inhibition effect of the pyruvate on glycolytic pathway key enzymes is weakened, and consequently pyruvate yield is increased, and production intensity is improved. By means of genetic engineering technology, a signal peptide sequence Shrew1p and MPC1 derived from saccharomyces cerevisiae are positioned and integrated to a pY26 expression vector to construct a saccharomyces cerevisiae recombinant expression plasmid pY26-Shrew1p-MPC1 which is electrophoretically transferred into a recipient bacterium TgU to obtain an engineering strain TgU-(pY26-Shrew1p-MPC1) high in pyruvate yield. Compared with a reference strain TgU-(pY26), the engineering strain TgU-(pY26-Shrew1p-MPC1) has the advantage that dry cell weight, glucose consumption rate, pyruvate yield and pyruvate production intensity are increased by 58.3%, 68.1%, 154.4% and 152.6% respectively.
Owner:JIANGNAN UNIV
Industrial saccharomyces cerevisiae strain for synchronously producing xylitol and ethanol and construction method of industrial saccharomyces cerevisiae strain
PendingCN114134058AHigh copy numberIncrease consumption rateFungiBiofuelsConcentrations glucoseEthanol yield
The invention provides an industrial saccharomyces cerevisiae strain for synchronously producing xylitol and ethanol and a construction method of the industrial saccharomyces cerevisiae strain. The strain highly expresses an FPS1 gene and an XYL1 gene. Specifically, the strain is SEB20, the classification name of the strain is Saccharomyces cerevisiae, and the preservation number of the strain is CGMCC No.22590. The strain is obtained by taking the Saccharomyces cerevisiae SEB10 as an original strain, replacing a promoter PFPS1 of a gene FPS1 with a strong promoter PTDH3, and increasing the copy number of a CtXYL1 gene while knocking out two site gene coding regions of SOR1 and SOR2. Under the condition that the initial glucose concentration of the saccharomyces cerevisiae strain SEB20 is relatively high, the xylose consumption rate and the yield of xylitol and ethanol are improved. Under a test tube fermentation condition, compared with SEB10, the xylose consumption rate is increased by 73.5%, and the xylitol yield is increased by 27.0%. Under the conditions of straw pre-saccharification and simultaneous saccharification fermentation, compared with an original strain SEB10, the strain SEB20 has the advantages that the xylose consumption rate is increased by 24.1%, the xylitol yield is increased by 42.9%, the xylitol yield is close to a theoretical value 1.0, and the ethanol yield is increased by 5.0%.
Owner:CHINA PETROLEUM & CHEM CORP +2
Device and method for enhancing iron autotrophic denitrification coupling carbon capture
PendingCN114573102AIncrease consumption rateImprove removal rateWater contaminantsTreatment with anaerobic digestion processesEnvironmental chemistryGas supply
The invention relates to the technical field of wastewater denitrification and carbon emission reduction, in particular to a device and a method for enhancing iron autotrophic denitrification coupled carbon capture. The invention provides a device for enhancing iron autotrophic denitrification coupled carbon capture. The device comprises a water inlet device, a carbon dioxide supply system, a low-intensity magnetic field reactor and an adsorption liquid pool, the water inlet device is connected with a water inlet of the low-intensity magnetic field reactor; the carbon dioxide gas supply system is connected with a gas inlet of the low-intensity magnetic field reactor; the low-intensity magnetic field reactor is connected with the adsorption liquid pool through an exhaust port; the low-intensity magnetic field reactor comprises an iron-based carrier. The device can synchronously realize the purposes of denitrification and carbon emission reduction in the wastewater.
Owner:BEIJING JIAOTONG UNIV
High-strength coral aggregate concrete and its preparation method
ActiveCN108218350BReduce hydration temperature riseReduce or avoid temperature cracksAluminium chlorideKaolin clay
The invention discloses high-strength coral aggregate concrete which is prepared from the following ingredients in parts by weight: 260-350 parts of low-heat marine Portland cement, 35-50 parts of flyash floating beads, 35-50 parts of mineral powder, 8-16 parts of silica fume, 8-16 parts of metakaolin, 2-6 parts of nano silicon dioxide, 2-6 parts of polyaluminum chloride, 550-679 parts of coral reef sand, 890-1000 parts of coral reef and 180-256 parts of seawater. The high-strength coral aggregate concrete has the advantages that the pelagic engineering construction cost is lowered, and environment-friendly and energy-saving requirements of civil engineering are also met.
Owner:CCCC SECOND HARBOR ENG +2
A xylose fermentation strain tolerant to inhibitors and its construction method and application
ActiveCN109706089BIncreased ability to tolerate inhibitorsSuperior ability to tolerate inhibitorsFungiMutant preparationBiotechnologyXylose fermentation
Owner:CHINA PETROLEUM & CHEM CORP +2
Saccharomyces cerevisiae Genetically Engineered Bacteria Using Glucose Efficiently, Its Construction Method and Application
ActiveCN107325976BImprove fermentation effectIncrease consumption rateFungiTransferasesThiazoleMicrobiology
The invention discloses a saccharomyces cerevisiae genetically engineered bacterium capable of efficiently utilizing glucose. The genetically engineered bacterium is used for over-expressing thiazole synthase Thi4 and a global transcription factor Hap4 and belongs to the field of genetic engineering. The invention further discloses a construction method and fermentation application of a strain. According to the saccharomyces cerevisiae genetically engineered bacterium disclosed by the invention, a high-copy free type plasmid pYX212 carrier is utilized and endogenous thiazole synthase Thi4 and global transcription factor Hap4 are over-expressed in the saccharomyces cerevisiae strain to obtain a recombinant saccharomyces cerevisiae strain capable of improving the consumption speed of the glucose, the growth speed of thalli and the yield of ethanol.
Owner:NANJING TECH UNIV
Method for synthesizing microbial oils by two-stage fed-batch fermentation
The invention discloses a method for synthesizing microbial oils by two-stage fed-batch fermentation. Sugar is added into a fermentation medium as a carbon source; a Cryptococcus albidus seed solution is inoculated for fermentation culture; rotating speed of a fermentation cylinder is controlled to 100-900 r / m, fermentation temperature is controlled to 20-30 DEG C and pH is controlled to 5-7 until dissolved oxygen concentration and pH value rise; when sugar concentration is 1-5 g / L, volatile fatty acid mixed liquor begins to be fed to replace sugar as the carbon source, and nitrogen source restriction is implemented; and when concentration of the volatile fatty acid mixed liquor is 1-5 g / L, the volatile fatty acid mixed liquor is fed until the total volatile fatty acid mixed liquor concentration is 5-20 g / L. The rate of microbial metabolism of volatile fatty acids is high, the inhibition effect of high-concentration organic acid carbon source on cell growth is little, and then the final cell concentration and product concentration are both high. The method of the invention can effectively help reduce production cost of microbial oils.
Owner:XI AN JIAOTONG UNIV
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