42results about How to "Increase consumption rate" patented technology

Succinic acid is produced by allowing a bacterium modified to enhance fumarate reductase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. More preferably, succinic acid is produced by allowing a bacterium modified to enhance activities of fumarate reductase and pyruvate carboxylase and decrease lactate dehydrogenase activity or cell preparation thereof to react with an organic raw material in a reaction solution containing one of a carbonate ion, a bicarbonate ion, and carbon dioxide gas to generate succinic acid. Succinic acid is obtained by collecting the produced succinic acid.

The invention discloses an acetic acid bacterium and its application in fermenting apple cider vinegar, belonging to the field of industrial microorganisms. The acetic acid bacterium is a strain of Acetobacter pasteurianus (Acetobacter pasteurianus) CYD159, which was preserved in the China Center for Type Culture Collection on June 30, 2015. The preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2015418. The bacterium has high acid-producing ability, and the acid-producing ability reaches more than 110g / L. The fermented apple cider vinegar produced by using the acetic acid bacterium strain has high acetic acid yield and good flavor, and is an acetic acid bacterium suitable for brewing apple cider vinegar.

The invention relates to a method for culturing chlorella by heterotrophism with sludge hydrolysate, relating to a method for culturing the chlorella through heterotrophism. The invention aims at solving the problem that the existing microalgae biodiesel is high in cost. The method disclosed by the invention comprises the following steps of: firstly, carrying out ultrasonic processing on excess sludge of a domestic sewage treatment plant; secondly, carrying out anaerobic hydrolysis on the sludge for 2-3 days under the conditions that rotating speed is 180r / min and temperature is 35 DEG C, regulating the pH value to 9.0, and carrying out nitrogen stripping every 12 hours, so as to obtain the sludge hydrolysate, adding trace elements except nitrogen and phosphorus and other nutrition elements, and carrying out high pressure steam sterilization, so as to obtain a culture solution; and thirdly, inoculating the chlorella in percentage by volume of 10-20%, and then culturing for 150-180 hours in a shaking culture tank, thus the culture of the chlorella is completed. By adopting the method disclosed by the invention, the cost for producing the chlorella can be saved, and low-cost raw materials are provided for commercial production of biodiesel. The method disclosed by the invention is applied to the biodiesel preparation field.

Disclosed is a process for production of succinic acid, which comprises the step of reacting a bacterium which has been modified so as to increase the expression of a sucE1 gene or a product produced by any treatment of the bacterium with an organic raw material in a reaction solution containing a carbonate ion, a bicarbonate ion or carbon dioxide gas to thereby yield the desired succinic acid.

The invention relates to the technical field of body building and weight losing and in particular relates to an ice hot body building and weight losing method and equipment suitable for the same. The ice hot body building and weight losing method comprises the following specific step: simultaneously carrying out thermal stimulation and cold stimulation to two positions of a human body. The equipment suitable for the method comprises a tank body and a tank cover, wherein the lower part of the interior of the tank body is provided with a cold water tank; the upper part of the interior of the tank body is provided with a hot air chamber; and the cold water tank is communicated with the hot air chamber. When the cold water tank carries out cold stimulation on certain position of the human body, vessels of the whole body participate in vasomotor motion to increase the elasticity of the vessels, thereby bringing convenience for preventing and treating arteriosclerosis, hypertension and coronary heart disease, and performing the action on building the body. In addition, the human body is stimulated to quicken metabolism to resist freezing so as to improve the fat consumption rate of the human body; the hot air chamber is used for carrying out thermal stimulation on other positions of the human body; and the thermal environment is utilized to quicken the human body to perspire and quicken the fat to burn so as to finally realize the purpose of losing weight.

The invention belongs to the technical field of fermentation engineering and discloses a recombinant bacterium with a function of pyruvate production intensity improvement and application of the recombinant bacterium with the function of pyruvate production intensity improvement. By targeting expression of a mitochondrion pyruvate carrier protein to a cell membrane in a torulopsis glabrata strain, cell outgoing rate of pyruvate is increased while a feedback inhibition effect of the pyruvate on glycolytic pathway key enzymes is weakened, and consequently pyruvate yield is increased, and production intensity is improved. By means of genetic engineering technology, a signal peptide sequence Shrew1p and MPC1 derived from saccharomyces cerevisiae are positioned and integrated to a pY26 expression vector to construct a saccharomyces cerevisiae recombinant expression plasmid pY26-Shrew1p-MPC1 which is electrophoretically transferred into a recipient bacterium TgU to obtain an engineering strain TgU-(pY26-Shrew1p-MPC1) high in pyruvate yield. Compared with a reference strain TgU-(pY26), the engineering strain TgU-(pY26-Shrew1p-MPC1) has the advantage that dry cell weight, glucose consumption rate, pyruvate yield and pyruvate production intensity are increased by 58.3%, 68.1%, 154.4% and 152.6% respectively.

The invention discloses high-strength coral aggregate concrete which is prepared from the following ingredients in parts by weight: 260-350 parts of low-heat marine Portland cement, 35-50 parts of flyash floating beads, 35-50 parts of mineral powder, 8-16 parts of silica fume, 8-16 parts of metakaolin, 2-6 parts of nano silicon dioxide, 2-6 parts of polyaluminum chloride, 550-679 parts of coral reef sand, 890-1000 parts of coral reef and 180-256 parts of seawater. The high-strength coral aggregate concrete has the advantages that the pelagic engineering construction cost is lowered, and environment-friendly and energy-saving requirements of civil engineering are also met.

The invention discloses a xylose fermentation strain tolerant to inhibitors, which is named SEB11, and its preservation number is CGMCC No.16570; the invention also relates to a construction method of the above xylose fermentation strain, which includes the following steps: Select the starting strain SEB5; carry out plasma mutagenesis treatment on the starting strain, and select colonies with better or similar growth than the starting strain; carry out plate primary screening and re-screening of the actual saccharification solution for the selected bacterial strains, and obtain tolerance to inhibitors. Xylose fermenting strain SEB11; the present invention also relates to the application of the above xylose fermenting strain in producing ethanol by using xylose in lignocellulose. The present invention takes industrial strains that can utilize xylose as starting strains, utilizes atmospheric pressure and room temperature plasma mutagenesis technology to carry out mutagenesis, and according to the growth conditions in the plate culture medium containing inhibitors and the actual saccharification liquid fermentation re-screening, obtains The xylose fermentation mutant strain SEB11 with excellent inhibitor tolerance has a significantly increased xylose consumption rate, showing better fermentation results.

The invention discloses a saccharomyces cerevisiae genetically engineered bacterium capable of efficiently utilizing glucose. The genetically engineered bacterium is used for over-expressing thiazole synthase Thi4 and a global transcription factor Hap4 and belongs to the field of genetic engineering. The invention further discloses a construction method and fermentation application of a strain. According to the saccharomyces cerevisiae genetically engineered bacterium disclosed by the invention, a high-copy free type plasmid pYX212 carrier is utilized and endogenous thiazole synthase Thi4 and global transcription factor Hap4 are over-expressed in the saccharomyces cerevisiae strain to obtain a recombinant saccharomyces cerevisiae strain capable of improving the consumption speed of the glucose, the growth speed of thalli and the yield of ethanol.