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Method for culturing chlorella by heterotrophism with sludge hydrolysate

A chlorella and hydrolyzate technology, applied in microorganism-based methods, water/sludge/sewage treatment, sludge treatment, etc., can solve the problem of high cost of microalgae biodiesel, achieve resource utilization and save production cost effect

Inactive Publication Date: 2014-09-24
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of high cost of existing microalgae biodiesel, and to provide a method for cultivating chlorella heterotrophically using sludge hydrolyzate

Method used

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  • Method for culturing chlorella by heterotrophism with sludge hydrolysate
  • Method for culturing chlorella by heterotrophism with sludge hydrolysate
  • Method for culturing chlorella by heterotrophism with sludge hydrolysate

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specific Embodiment approach 1

[0015] Specific embodiment one: a kind of method of utilizing sludge hydrolyzate heterotrophic culture Chlorella of the present embodiment is to carry out according to the following steps: 1, sludge ultrasonic treatment: take the remaining sludge of domestic sewage treatment plant, the sewage The moisture content of the mud is concentrated to a mass percentage of 98%~98.5%, and the ultrasonic treatment is carried out for 8~10min under the condition of an acoustic energy density of 5~10W / mL and a pulse ratio of 2:1; 2. Sludge hydrolyzate Preparation: The sludge after the ultrasonic treatment in step 1 was subjected to anaerobic hydrolysis for 2~3 days under the conditions of rotation speed of 180r / min and temperature of 35°C. During the hydrolysis process, the pH value was adjusted to 9.0 every 12h, and At nitrogen flow rate of 0.3m 3 Under the condition of / h, carry out a nitrogen blow-off every 12h to obtain the sludge hydrolyzate; 3. Add trace elements and other nutritional ...

specific Embodiment approach 2

[0018] Embodiment 2: This embodiment is different from Embodiment 1 in that: the pH value adjustment described in step 2 is carried out with 1 mol / L NaOH. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0019] Embodiment 3: This embodiment differs from Embodiments 1 to 2 in that the operating conditions of the high-pressure steam sterilization described in Step 3 are: 121° C., 121 KPa, sterilizing for 30 minutes. Others are the same as the specific embodiment 1 to 2.

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Abstract

The invention relates to a method for culturing chlorella by heterotrophism with sludge hydrolysate, relating to a method for culturing the chlorella through heterotrophism. The invention aims at solving the problem that the existing microalgae biodiesel is high in cost. The method disclosed by the invention comprises the following steps of: firstly, carrying out ultrasonic processing on excess sludge of a domestic sewage treatment plant; secondly, carrying out anaerobic hydrolysis on the sludge for 2-3 days under the conditions that rotating speed is 180r / min and temperature is 35 DEG C, regulating the pH value to 9.0, and carrying out nitrogen stripping every 12 hours, so as to obtain the sludge hydrolysate, adding trace elements except nitrogen and phosphorus and other nutrition elements, and carrying out high pressure steam sterilization, so as to obtain a culture solution; and thirdly, inoculating the chlorella in percentage by volume of 10-20%, and then culturing for 150-180 hours in a shaking culture tank, thus the culture of the chlorella is completed. By adopting the method disclosed by the invention, the cost for producing the chlorella can be saved, and low-cost raw materials are provided for commercial production of biodiesel. The method disclosed by the invention is applied to the biodiesel preparation field.

Description

technical field [0001] The invention relates to a method for heterotrophically cultivating chlorella. Background technique [0002] The depletion of fossil fuels is the focus of global attention, and existing science and technology cannot solve the current energy demand. The high use of crude oil leads to an increase in the price of fossil fuels (of which the transportation system is the main consumer), and at the same time, causes environmental problems due to the increase in greenhouse gas emissions. The development of renewable energy has become a research hotspot in the field of energy and environment, such as solar energy, biomass energy, geothermal energy, water and wind energy, among which, biomass energy. Because of the renewable, biodegradable and non-toxic nature of renewable energy, it has become a very potential energy source, and has a large number of resource sources, such as agricultural and animal husbandry waste, municipal waste, industrial waste , terrest...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C02F11/00C02F11/10C02F11/06C02F11/02C12R1/89
CPCY02W10/33Y02W10/37Y02W10/40
Inventor 温沁雪陈志强李硼飞
Owner HARBIN INST OF TECH
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