Method for efficiently producing rare earth element reinforced 2-keto-L-gulonic acid

A rare earth element, ketoguronic acid bacteria technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc.

Inactive Publication Date: 2010-03-17
JIANGNAN UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of studies have shown that rare earth elements can promote or inhibit some substance metabolism processes and enzyme activities in body

Method used

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  • Method for efficiently producing rare earth element reinforced 2-keto-L-gulonic acid
  • Method for efficiently producing rare earth element reinforced 2-keto-L-gulonic acid

Examples

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Example Embodiment

[0032] Example 1: Adding cerium ions (Ce 3+ ) When the concentration reaches 5mmol / L, such as figure 1 After 60 hours of fermentation, the concentration of 2KGA and L-sorbose reached 72.1g· L-1 ,8.4g·L -1 , The average sorbose consumption rate and 2KGA production rate were increased by 23.5% and 27.1% respectively compared with the control group. Continue to increase the cerium ion (Ce 3+ ) At the concentration, the output of 2KGA will decrease.

Example Embodiment

[0033] Example 2: Adding lanthanum ions (La 3+ ) When the concentration reaches 7mmol / L, such as figure 2 , The concentration of 2KGA and L-sorbose reached 75.7g·L after 60h fermentation -1 ,8.9g·L -1 , The sugar acid conversion rate increased from 83.1% to 91.6%, and the average sorbose consumption rate and 2KGA production rate increased by 22.8% and 35.3% respectively. Continue to increase the lanthanum ion (La 3+ ) At the concentration, the output of 2KGA will decrease.

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Abstract

The invention relates to a method for efficiently producing rare earth element reinforced 2-keto-L-gulonic acid, belonging to the technical field of fermentation processes of optimized vitamin C. Themethod utilizes a mixed fungus strain of ketogulonigenium vulgare and bacillus megaterium as a production fungus strain and prompts the production of the 2-keto-L-gulonic acid (2KGA) and the consumption of sorbose by adding cerium ions Ce<3+> or lanthanum ions La<3+> into a culture medium so as to realize the efficient production of the 2KGA. Due to the addition of 5mmol/L of the cerium ions, theoutput of the 2KGA is improved to 72.1g/L from 67.9g/L of a contrast embodiment, the fermentation period is shortened for about 12h, and the average consumption rate of the sorbose and the generationrate of the 2KGA are respectively improved for 23.5 percent and 27.1 percent. Due to the addition of 7mmol/L of the lanthanum ions, the output of the 2KGA is improved to 75.7g/L, the fermentation period is shortened for about 12h, the conversion rate from the sorbose to the 2KGA is improved from 83.1 percent to 91.6 percent, and the average consumption rate of the sorbose and the generation rate of the 2KGA are respectively improved by 22.8 percent and 35.3 percent.

Description

technical field [0001] The invention discloses a method for enhancing the high-efficiency production of 2-keto-L-gulonic acid (2KGA) with rare earth elements, and relates to the technical field of optimizing vitamin C fermentation process. Background technique [0002] Vitamin C (Vitamin C, hereinafter referred to as VC), also known as L-ascorbic acid, is an essential water-soluble amino acid with a wide range of physiological functions and physical and chemical properties, so it has important uses in the fields of medicine, food and feed. At the same time, VC is also the variety with the fastest development, the largest output and the widest application among vitamin drugs. At present, the production of VC in my country mainly adopts the "two-step fermentation method". megaterium, commonly known as "big bacteria") and other associated bacteria to convert L-sorbose into VC precursor 2-keto-L-gulonic acid (2KGA). [0003] Rare earth elements are also called rare earth metals...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P7/60C12R1/11C12R1/01
Inventor 刘立明郑巧双刘杰周景文陈坚
Owner JIANGNAN UNIV
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