35 results about "Ketogulonigenium" patented technology

The invention relates to a method for improving 2-keto-L-gluconic acid fermentation efficiency. The method is characterized in that in the process of mixed strains fermenting 2-keto-L-gluconic acid, the mixed strains comprise ketogulonigenium vulgare and bacillus megaterium, seed solution cultured to the middle and later periods of logarithmic growth is inoculated into fermentation tank optimum culture media to ferment and culture, sorbose is fed since fermentation time is 0h, and small material culture media are fed at the prior period of the logarithmic growth. According to the method, production intensity of the 2-keto-L-gluconic acid can be improved, efficiency is improved, production capacity is optimized, the content of the 2-keto-L-gluconic acid in fermentation solution can reach 115.6-150.3mg / ml, acid producing rate is 2.89-3.76g.(L.h)-1, and the conversion rate of glucose acid is 87.2-95.0%.

The invention provides a method for promoting growth and acid generation of Ketogulonigenium vulgare. By adding trophic factor lipoic acid into a Ketogulonigenium vulgare culture medium, the growth rate and the acid production capacity of the Ketogulonigenium vulgare can be improved, thereby the object that a single bacterium fermentation method realizes sugar and acid conversion is achieved, andthe fermentation process of vitamin C is simplified.

The invention discloses a medium for cordyceps militaris liquid deep fermentation. The medium comprises 10-30g of cane sugar, 1-5g of corn steep liquor, 4-100g of fermenting mash protein residue, 0-4g of peptone, 0-4g of beef extract, 0.5-2.0g of MgSO4.7H2O, 0.2-1.0g of KH2PO4, 1-5mg of vitamin B1 and 1000ml of water. The medium contains waste fermenting mash protein residue obtained by vitamin C fermentation, the fermenting mash protein residue contains mycoprotein of bacillus megatherium and ketogulonigenium vulgare, and the mycoprotein is similar to silkworm pupa protein and thus the mycoprotein can completely or partially replace beef extract and peptone in the medium. The medium can improve a cordyceps militaris mycelium fermentation yield, realize resource utilization of the waste fermenting mash protein residue obtained by a vitamin C fermentation industry and effectively reduce a production cost. The invention also provides a use method of the medium for cordyceps militaris liquid deep fermentation.

The invention belongs to the technical field of vitamin C biological fermentation, and particularly relates to a method using three-bacterium mixed fermentation to convert sorbose into 2-keto-L-gulonic acid which is in a second step of the vitamin C biological fermentation. Seed solution of manufacturing technique of the second step of the vitamin C biological fermentation is enlarged step by step, when in fermental cultivation, with total inoculation amount with volume percent of 15-20%, seed solution of A isolates and B isolates are introduced into a fermentation tank to be fermented and cultivated to a fermentation ending point (namely when the concentration of remained sorbose in fermentation liquor is smaller than or equal to 1mg / ml), and the purpose that the mixed bacteria fermentation is adopted for converting the sorbose to the 2-keto-L-gulonic is achieved. The A isolates is composed of companion fungus bacillus cereus / companion fungus bacillus megatherium and acid-forming bacteria ketogenic base ketogulonigenium vulgare. The B isolates is composed of companion fungus bacillus thuringiensis and acid-forming bacteria ketogenic base ketogulonigenium vulgare. According to the novel method using the three-bacterium mixed fermentation, respective advantages of the two kinds of the companion funguses are made full use, fermentation period can be shortened by 6-8 hours, energy consumption is reduced by 10-15%, and fermentation conversion percent is improved by 2-4%.

The invention discloses a medium for cordyceps militaris liquid deep fermentation. The medium comprises 10-30g of cane sugar, 1-5g of corn steep liquor, 4-100g of fermenting mash protein residue, 0-4g of peptone, 0-4g of beef extract, 0.5-2.0g of MgSO4.7H2O, 0.2-1.0g of KH2PO4, 1-5mg of vitamin B1 and 1000ml of water. The medium contains waste fermenting mash protein residue obtained by vitamin C fermentation, the fermenting mash protein residue contains mycoprotein of bacillus megatherium and ketogulonigenium vulgare, and the mycoprotein is similar to silkworm pupa protein and thus the mycoprotein can completely or partially replace beef extract and peptone in the medium. The medium can improve a cordyceps militaris mycelium fermentation yield, realize resource utilization of the waste fermenting mash protein residue obtained by a vitamin C fermentation industry and effectively reduce a production cost. The invention also provides a use method of the medium for cordyceps militaris liquid deep fermentation.

The invention relates to a method utilizing various microorganisms for co-culture or coupling fermentation. With difference from a relationship that vitamin C fermentation bacillus megaterium is mainly used for assisting ketogulonigenium vulgare to grow and produce acids, the method mainly utilizes a method utilizing various microorganisms for co-culture or coupling fermentation to overcome metabolic regulation obstacles for producing fermentation products. The co-culture comprises that two or more than two microorganism strains are subjected to mixed culture from the beginning, or firstly one strain is cultured, and when the strain is cultured to a proper time, other strains are added for culture.

The invention provides a mutation screening method of ketogulonigenium vulgare. The ketogulonigenium vulgare in the vitamin C fermentation production is used as an original strain, low-temperature normal-pressure plasma mutation equipment is adopted for mutation, a solid medium is coated with the ketogulonigenium vulgare, single colonies evenly grow out, single-bacterium multiplication and enlarged culture continue to be carried out, and target bacterial strains are screened out through a companion fungus shaking bottle. By means of the technology, the mutation screening parallelity of the ketogulonigenium vulgare is improved, and the probability of obtaining efficient bacterial strains is increased.

The invention discloses a method for promoting L-sorbose to convert to generate 2-keto-L-gulonic acid, belonging to the technical field of microbial fermentation. The method comprises two steps of seed cultivation and fermentative cultivation; according to the method, astragalus extract in an additive amount of 0.75-2.00g / L or gastrodia elata extract in an additive amount of 1.50-3.00g / L is added in a fermentation medium, or the astragalus extract and the astrodia elata extract in additive amounts of 1.00-1.50g / L and 1.50-2.00g / L are added in the fermentation medium at the same time, so as to promote the common ketogulonigenium vulgare (small bacterium) and huge bacillus (large bacterium) to coordinately grow to realize the efficient synthesis of the 2-keto-L-gulonic acid; and through coordinating the mutual functions of the two bacteria, the growth of the small bacterium is promoted, the output of the 2-keto-L-gulonic acid is improved and the fermentation period is shortened. According to the method, a 10L tank is used for the fermentation experiment and a predicted effect is obtained.

The invention discloses a method for improving the production intensity of 2-keto-L-gulonic acid (2-KLG) by adding betaine, belonging to the technical field of vitamin C preparation through fermentation. A mixed bacteria system of ketogulonigenium vulgare and bacillus megaterium is adopted as a production strain, and 0.05-1.0% betaine is exogenously added into a fermentation medium as an osmotic pressure regulator so as to promote the cell growth and 2-KLG production and realize efficient production of 2-KLG. By adopting the process, the method disclosed by the invention can effectively shorten the fermentation period of 2-KLG and improve the production intensity.

The invention discloses a method for fermenting and producing vitamin C precursor 2-keto-L-gulonic acid, which comprises Bacillus licheniformis (associated bacteria) overexpressing D-sorbitol dehydrogenase (SLDH) Mixed culture with acid-producing bacteria Ketogulonigenium vulgare, fermented to produce 2‑keto‑L‑gulgare. The method of the invention realizes the "one-pot method" conversion from D-sorbitol to 2-keto-L-gulonic acid, simplifies the production process, shortens the fermentation period, and effectively reduces the production cost.

The invention discloses a method for transforming Escherichia coli to produce vitamin C precursor 2-keto-L-gulonic acid, which belongs to the field of genetic engineering. In the present invention, through genetic engineering technology, sorbitol dehydrogenase (SLDH), coenzyme pyrroloquinoline quinone (Pyrroloquinoline quinone, PQQ) gene cluster derived from Gluconobacter oxydans and common ketogenic gluconic acid The sorbose dehydrogenase (SDH) / sorbone dehydrogenase (SNDH) gene of Ketogulonigenium vulgare was expressed in Escherichia coli, and a strain of E.coli that utilizes sorbitol to produce 2-KLG was obtained. bacteria. At present, the two-step fermentation process used in the industrial production of vitamin C in China is complicated, with many influencing factors, and it is difficult to control accurately. At the same time, the growth of three kinds of microorganisms consumes a lot of raw materials and energy. E.coli engineering bacteria are used for fermentation production with sorbitol 2-KLG simplifies the production process, saves raw materials and energy, and the output of 2-KLG can reach 87g / L, which has a good application prospect.

The invention discloses a Gluconobacter oxydans engineering bacterium for producing vitamin C synthesis intermediate sorbic ketone and a construction method and application thereof, and belongs to the field of genetic engineering. By means of the genetic engineering technique, a sorbose dehydrogenase gene (sdh) derived from common ketogenic base Ketogulonigenium vulgare is cloned onto Gluconobacter oxydans to obtain the G. oxydans engineering bacterium for producing the sorbic ketone by utilizing sorbitol. G. oxydans WSH-003 which is provided by Jiangsu Jiangshan Pharmaceutical Co., Ltd is a fermented industrial strain obtained in a first step of a two-step fermentation method for producing 2-keto-L-gulonic acid (2-KLG). The sdh is cloned into the G. oxydans so that conversion from D-sorbitol to the vitamin C synthesis intermediate sorbic ketone is achieved, the foundation for further constructing the fermented industrial strain in one step to produce the direct precursor 2-KLG of vitamin C is laid, and the yield of the sorbic ketone is as high as 72g / L. Therefore, the Gluconobacter oxydans engineering bacterium has good application prospects.