Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for promoting L-sorbose to convert to generate 2-keto-L-gulonic acid

A technology of gulonic acid and sorbose is applied in the field of efficient synthesis of 2-keto-L-gulonic acid, promoting the conversion of L-sorbose to generate 2-keto-L-gulonic acid, and achieving increased yield and promotion of bacterial growth. , the effect of shortening the fermentation cycle

Active Publication Date: 2014-09-17
石药集团维生药业(石家庄)有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The extracts of Astragalus and Gastrodia elata have certain effects on the growth of microorganisms, but so far there are no research reports on the effects of the two kinds of Chinese herbal extracts on the efficient synthesis of 2-keto-L-gulonic acid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for promoting L-sorbose to convert to generate 2-keto-L-gulonic acid
  • Method for promoting L-sorbose to convert to generate 2-keto-L-gulonic acid
  • Method for promoting L-sorbose to convert to generate 2-keto-L-gulonic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] In order to better illustrate the present invention, the following examples are cited. However, the scope of the present invention is not limited thereto, and the scope of protection is described in the claims.

[0017] Examples 1-9, by using 0.00g / L, 0.50g / L, 0.75g / L, 1.00g / L, 1.25g / L, 1.50g / L, 1.75g / L, 2.00 g / L respectively in the fermentation medium g / L, 2.50 g / L addition amount M (g / L) add astragalus extract, and produce 2-keto by the following "method of promoting conversion of L-sorbose to 2-keto-L-gulonic acid" -L-gulonic acid, the steps are as follows:

[0018] 1. Seed cultivation

[0019] Weigh the ingredients of the seed medium according to the formula to prepare the seed medium (g / L): L-sorbose 20, yeast extract 3, beef extract 3, peptone 10, corn steep liquor 1.5, urea 1, KH 2 PO 4 1. MgSO 4 0.2, CaCO 3 1. Adjust the pH to 6.5-7.0, and sterilize at 121°C for 20 minutes;

[0020] Scrape a ring of mixed bacteria with a ring size and inoculate the law...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for promoting L-sorbose to convert to generate 2-keto-L-gulonic acid, belonging to the technical field of microbial fermentation. The method comprises two steps of seed cultivation and fermentative cultivation; according to the method, astragalus extract in an additive amount of 0.75-2.00g / L or gastrodia elata extract in an additive amount of 1.50-3.00g / L is added in a fermentation medium, or the astragalus extract and the astrodia elata extract in additive amounts of 1.00-1.50g / L and 1.50-2.00g / L are added in the fermentation medium at the same time, so as to promote the common ketogulonigenium vulgare (small bacterium) and huge bacillus (large bacterium) to coordinately grow to realize the efficient synthesis of the 2-keto-L-gulonic acid; and through coordinating the mutual functions of the two bacteria, the growth of the small bacterium is promoted, the output of the 2-keto-L-gulonic acid is improved and the fermentation period is shortened. According to the method, a 10L tank is used for the fermentation experiment and a predicted effect is obtained.

Description

technical field [0001] The invention relates to a method for promoting the transformation of L-sorbose into 2-keto-L-gulonic acid, in particular to a method for promoting common ketogenic radical by adding astragalus, gastrodia elata or extracts of astragalus and gastrodia elata into the fermentation medium. A method for synthesizing 2-keto-L-gulonic acid through the coordinated growth of Bacillus couronicum (small bacteria) and Bacillus megaterium (big bacteria). It belongs to the technical field of microbial fermentation. Background technique [0002] Vitamin C (Vitamin C, referred to as Vc), also known as L-Ascorbic acid (L-Ascorbic acid), is an essential vitamin for the human body. It has a wide range of physiological functions and plays an important role in the pharmaceutical and food industries. At present, the "two-step fermentation method" independently developed by China is widely used in my country. The first step of the "two-step fermentation method" is single-ba...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/60C12R1/01C12R1/11
Inventor 冯志军焦迎晖王清格马乐辉安卫红李会然王俊芬
Owner 石药集团维生药业(石家庄)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com