16 results about "Gulonic acid" patented technology

The invention discloses a nutrient solution capable of increasing vitamin C of plants and a preparation method of the nutrient solution, and relates to the technical field of plant nutrition and fertilizers. The nutrient solution contains additives such as 2-keto-L-gulonic acid or 2-keto-L-gulonic acid salt and the like. According to the invention, 2-keto-L-gulonic acid or 2-keto-L-gulonic acid sodium or 2-keto-L-gulonic acid potassium and the like are used as core raw materials of the nutrient solution for plants, and are compounded with basic nutrient raw materials and additives required by the plants to prepare the plant nutrient solution and the composite nutrient solution. The 2-keto-L-gulonic acid and the 2-keto-L-gulonate in the plant body can be catalyzed and converted into the vitamin C, so that the content of the vitamin C in the tree body is remarkably increased.

The present invention relates to a method for preparing vitamin C. The method comprises: dissolving 2-keto-L-gulonic acid in water to obtain a keto-gulonic acid solution; adding a catalyst composed of palladium acetate, citric acid, and L-alanine to the keto-gulonic acid solution; reacting at 30-35 degrees Celsius for 1-2 hours; heating to 50-55 degrees Celsius; and after the reaction is carried out for 20 hours, stopping heating and unloading to obtain a solid liquid containing vitamin C; cooling the solid liquid containing vitamin C at room temperature for crystallization for 4-6 hours, and then using centrifugal separation and purification to obtain vitamin C. The process for preparing vitamin C using the scheme of the present invention is simpler and more efficient.

Disclosed herein are improved pluripotent stem cell-derived multi-zonal liver organoids, having two or more hepatocyte subpopulations, and methods of producing the same, by coculturing a first human liver organoid (HLO) with a bilirubin-treated second HLO, and contacting the co-cultured HLOs with bilirubin for a period of time sufficient for the co-cultured HLOs to self-assemble and fuse into interconnected dual organoids. The first HLO can be an ascorbateenriched HLO, such as a functional L-gulonolactone oxidase (GULO)-expressing HLO. In particular embodiments, the hepatocyte subpopulations can include zone 1 and zone 3 hepatocyte subpopulations, or zone 1, zone 2, and zone 3 hepatocyte subpopulations. Also disclosed are methods of studying or treating a liver-related disease or disorder using the described multi-zonal liver organoids.

The invention relates to a method for producing 2-KGA through one-step fermentation of ketogulonigenium vulgare, and belongs to the technical field of fermentation engineering. In earlier-stage research, an inventor obtains a strain of ketogulonigenium sp. WTF0114 through mutagenesis screening, and accidentally finds that the strain can be directly converted into 2-keto-L-gulonic acid (2-KGA) by using D-sorbitol in one step, so that D-sorbitol is directly converted into 2-keto-L-gulonic acid by using the ketogulonigenium sp. WTF0114 and adopting a single-bacterium one-step fermentation method. Compared with a mainstream three-bacterium two-step fermentation method, the production process can be greatly simplified, the fermentation cost is reduced, the production period is shortened, and good economic benefits are achieved.

The present application relates to the technical field of separation and purification, and discloses a method for separating and purifying gulonic acid in fermentation liquor by salting-out extraction and coupling crystallization, which comprises the following steps: preparing a salting-out system by using organic solvents (ethanol, isopropyl alcohol, tert-butyl alcohol, n-butyl alcohol and acetone) and inorganic salts (ammonium sulfate, sodium chloride, sodium carbonate and sodium bicarbonate), and then carrying out salting-out extraction on the fermentation liquor, so that the gulonic acid can be effectively enriched in the lower phase, and the impurities such as bacteria, most of the pigments and impure proteins can be removed, thus making the gulonic acid easy to separate and purify.

The invention relates to the technical field of medicine preparation, in particular to a method for refining sodium ascorbate. The crude sodium ascorbate prepared by gulonic acid ester conversion is used as a raw material, and the high-purity sodium ascorbate finished product is prepared through two times of crystallization refining, so that refining steps of ion exchange, concentration and the like of the raw material vitamin C, which are long in time consumption and high in energy consumption, are omitted, and the preparation period of the finished sodium ascorbate finished product is greatly shortened; a new thought is provided for preparation of a sodium ascorbate finished product.

The invention relates to an alpha-glucosidase inhibitor and application thereof. The alpha-glucosidase inhibitor is vitamin C, dehydrogenated vitamin C, and 2, 3-diketone-L-gulonic acid. The influence of vitamin C, dehydrovitamin C and 2, 3-diketone-L-gulonic acid on alpha-glucosidase and HepG2 cell glucose consumption is researched through an in-vitro model, and the result shows that the vitamin C, dehydrovitamin C and 2, 3-diketone-L-gulonic acid can remarkably inhibit the activity of alpha-glucosidase and promote uptake and utilization of glucose by cells, so that the effect of improving insulin resistance is achieved.

The application discloses a copper 2,3-diketo-L-gulonic acid complex and a preparation method and application thereof, and belongs to the technical field of metal complexes and bioactive small molecules. The copper 2,3-diketo-L-gulonic acid complex is formed by coordination of 2,3-diketo-L-gulonic acid and copper ions. The complex can have an insertion type interaction with DNA and mediate DNA damage, and realizes multiple bioactivities of antibacteriophage, antibacterial and antitumor by virtue of the core action mechanism, has multiple application scenes, remarkable effects and a wide application range.

The invention belongs to the technical field of agricultural hydrogel development, and particularly relates to hydrogel for slowly releasing plant biostimulant as well as a preparation method and application of the hydrogel. The hydrogel is obtained by crosslinking the polyglutamic acid and the 2-keto-L-gulonic acid fermentation liquor. The hydrogel disclosed by the invention is relatively high in mechanical strength, has relatively good adsorption performance on soil moisture and shows good water storage and retention capacity, so that the use amount of irrigation water is effectively reduced; besides, the hydrogel can continuously release glutamic acid, 2-keto-L-gulonic acid, D-gulonic acid-gamma-lactone, citric acid and other micromolecular organic matters with the plant biostimulant function in the soil microenvironment, the content of quick-acting organic nutrients in soil is effectively increased while the water retention effect is achieved, the microbial activity is enhanced, and the soil remediation effect is improved. The soil conditioner can improve crop rhizosphere soil and strengthen the synergistic effect of water and fertilizer, thereby effectively promoting crop growth, yield increase and vitamin C accumulation, and has good application value in the fields of agriculture and environment.

This invention belongs to the fields of genetic engineering and bioengineering technology, specifically disclosing a method for synthesizing 2-keto-L-gulonic acid (2-KLG) using Bacillus as a substrate strain. This invention achieves single-strain, one-step fermentation for 2-KLG production using Bacillus as a substrate strain by introducing and expressing sorbitol dehydrogenase and sorbitol dehydrogenase with NAD(P)H or FADH as coenzymes in Bacillus. This invention provides a novel technical route for the microbial fermentation production of 2-KLG, with the potential to replace the traditional two-step fermentation process. It also provides reference and inspiration for the exploration and optimization of biosynthetic pathways for other important compounds.

The invention relates to the technical field of microbial breeding and fermentation engineering, in particular to an L-sorbose-resistant associated bacterium Y31 and application thereof in fermentation of 2-keto-L-gulonic acid. The companion fungus Y31 is classified and named as bacillus endophyticus, and the preservation registration number of the companion fungus Y31 is CCTCC (China Center For Type Culture Collection) NO: M20251995. The strain can normally grow in a culture medium with high L-sorbose content, and can significantly improve the yield of 2-keto-L-gulonic acid (2-KLG) when mixed with acid-producing bacteria for fermentation, shorten the fermentation period and improve the production efficiency of the vitamin C precursor 2-keto-L-gulonic acid.

The present application belongs to the field of agricultural planting, and particularly relates to a microbial synergistic plant planting method in heavy metal contaminated soil. The planting method comprises the following steps: planting plants mainly in the form of crops in the heavy metal contaminated soil to be repaired; applying 2-keto-L-gulonic acid mother liquor, plant growth promoting bacteria and plant biological stimulants to the soil around the roots and / or the leaves of the plants in the vegetative growth stage of the plants; and applying the gulonic acid mother liquor and endophytic bacillus in the reproductive growth stage of the plants. In the vegetative growth stage of the crops, the 2-keto-L-gulonic acid mother liquor, the plant growth promoting bacteria and the plant biological stimulants are applied, and in the reproductive growth stage, the 2-keto-L-gulonic acid mother liquor and the endophytic bacillus are applied, which not only greatly improves the heavy metal enrichment efficiency of the roots, stems and leaves of the crops, but also greatly reduces the heavy metal concentration of the seeds of the crops and meets the national feed or food standards.

The present application relates to a kind of crude vitamin C crystallization mother liquor component recovery method, first determine the content of vitamin C and gulonic acid in crude vitamin C mother liquor, determine the density of the mixture after evaporation of scraper film evaporator discharge;Active carbon is added to the crude vitamin C mother liquor, and decolorization is filtered;The decolorized mother liquor is sent to scraper film evaporator, and evaporated under reduced pressure;The mother liquor after evaporation is sent to crystallization tank and crystallized by cooling, the solubility of vitamin C and gulonic acid is different, the water in the mother liquor is removed by uniform evaporation, the concentration of VC and gulonic acid two components grows in proportion, wherein VC is saturated first and part of crystallization comes out, then gulonic acid just reaches saturation by cooling and no crystal is precipitated. High-purity VC crude product is obtained by cooling and centrifugation, and VC crude product is dissolved and added to the main line of production, and gulonic acid is transferred to the mother liquor for subsequent treatment.

The present invention relates to an important intermediate in the production process of vitamin C - L-2-keto-gulonic acid, and in particular to a concentration and crystallization process of gulonic acid. The method of the present invention comprises the following steps: concentrating the gulonic acid nanofiltrate by reduced pressure evaporation in an evaporator, the concentration condition being that the relative vacuum degree is greater than or equal to 0.090 MPa, concentrating to an end point solid content of 50-62%, cooling and crystallizing, and washing the crystals with low-temperature water during centrifugation. The mother liquor washed with water is directly concentrated and crystallized to obtain a dry mother liquor. The present invention solves the current situation of large-scale use and recovery of methanol in the production of gulonic acid, and high explosion-proof grade of production equipment and regions, improves the quality of the intermediate gulonic acid, reduces production costs, and improves production safety.

The invention belongs to the field of agricultural planting, and particularly relates to a method for planting plants in heavy metal contaminated soil through microorganism synergy. The planting method comprises the following steps that plants mainly including crops are planted in the heavy metal contaminated soil to be repaired; 2-keto-L-gulonic acid mother liquor, plant growth-promoting bacteria and plant biostimulant are applied to soil around roots and / or leaf surfaces of the plants in the vegetative growth stage of the plants; and applying the gulonic acid mother liquor and the plant endophytic bacillus to soil and / or leaves around roots of the plants in the reproductive growth stage of the plants. According to the method, the 2-keto-L-gulonic acid mother liquor, the plant growth-promoting bacteria and the plant biostimulant are applied in the vegetative growth stage of crops, and the 2-keto-L-gulonic acid mother liquor and the plant endophytic bacillus are applied in the reproductive growth stage, so that the heavy metal enrichment efficiency of roots, stems and leaves of the crops is greatly improved; and meanwhile, the heavy metal concentration of crop seeds is greatly reduced, and the national feed or food standard is met.

The application belongs to the technical field of agricultural hydrogel development, and particularly relates to a slow-release plant biological stimulant hydrogel and a preparation method and application thereof. The hydrogel is obtained by cross-linking polyglutamic acid and 2-keto-L-gulonic acid fermentation liquor. The hydrogel has high mechanical strength and good soil moisture adsorption performance, and shows good water storage and retention capacity, thereby effectively reducing the irrigation water consumption. In addition, the hydrogel can continuously release small-molecule organic matters with plant biological stimulant function such as glutamic acid, 2-keto-L-gulonic acid, D-gulonic acid-gamma-lactone and citric acid in the soil microenvironment, effectively improves the content of the soil available organic nutrients, enhances the microbial activity, improves the crop rhizosphere soil, strengthens the water and fertilizer synergistic effect, thereby effectively promotes the growth of crops, improves the yield and accumulates vitamin C, and has good application value in the fields of agriculture and environment.