161 results about "Gulonic acid" patented technology

A method for improving the stability for the fermentation production of 2-keto-L-gulonic acid belongs to the technical field of bioengineering. The 2-keto-L-gulonic acid is also named as vitamin C. The invention uses three mixture liquids to replace corn pulp, adopts an orthogonal test to investigate the important effects of a metal ion mixture liquid, an amino acid mixture liquid and a vitamin mixture liquid in the fermentation of the vitamin C. Based on the obtaining that the metal ion mixture liquid is the most remarkable affecting factor for cell growing, KGA synthesizing and L-sorbose consumption, the method of the invention further adopts the orthogonal test to investigate the effects of various metal irons to the fermentation of the vitamin C and discovers that increasing the concentration of Mg<2+> can promote the synthesizing of the KGA and relates to the most remarkable affecting factor for the fermentation of the vitamin C. The output and yield of the KGA which adopts optimized combination to carry out the fermentation of the vitamin C are respectively to be 75.2g/L and 0.94g/g. Compared with a common culture medium, the output (67g/L) and the yield (0.84g/g) of the KGA are respectively improved by 12.24 percent and 11.9 percent.

The invention discloses a gulonic acid adsorption separation method utilizing hyper-crosslinked resin. According to the gulonic acid adsorption separation method utilizing the hyper-crosslinked resin, a preprocessed gulonic acid fermentation broth is subjected to the processes of absorption, impurity washing and desorption through an adsorbing column filled with the hyper-crosslinked resin, and finally a gulonic acid solution is obtained. The hyper-crosslinked resin is prepared through a Friedel-Crafts cross-linking reaction and with polystyrene-divinyl benzene as a framework, and is weak in polarity and good in hydrophilia; the average grain diameter is 0.42-0.56mm, the water content is 43wt%, the bore diameter is 1.92nm, the porosity is 30-40%, the wet density 1.09g/cm3, the average specific surface area is 1645.5m2/g, the average pore volume is 0.732cm3/g, and a functional group is a carbanyl group. The adsorption medium adopted by the method has the advantages of being large in adsorption capacity for the gulonic acid, good in adsorptive selectivity, mild in desorption condition and long in service cycle, enabling regeneration to be easy and the like.