9 results about "Gulonic acid" patented technology

The invention relates to the technical field of medicine preparation, in particular to a method for refining sodium ascorbate. The crude sodium ascorbate prepared by gulonic acid ester conversion is used as a raw material, and the high-purity sodium ascorbate finished product is prepared through two times of crystallization refining, so that refining steps of ion exchange, concentration and the like of the raw material vitamin C, which are long in time consumption and high in energy consumption, are omitted, and the preparation period of the finished sodium ascorbate finished product is greatly shortened; a new thought is provided for preparation of a sodium ascorbate finished product.

The invention relates to an alpha-glucosidase inhibitor and application thereof. The alpha-glucosidase inhibitor is vitamin C, dehydrogenated vitamin C, and 2, 3-diketone-L-gulonic acid. The influence of vitamin C, dehydrovitamin C and 2, 3-diketone-L-gulonic acid on alpha-glucosidase and HepG2 cell glucose consumption is researched through an in-vitro model, and the result shows that the vitamin C, dehydrovitamin C and 2, 3-diketone-L-gulonic acid can remarkably inhibit the activity of alpha-glucosidase and promote uptake and utilization of glucose by cells, so that the effect of improving insulin resistance is achieved.

The application discloses a copper 2,3-diketo-L-gulonic acid complex and a preparation method and application thereof, and belongs to the technical field of metal complexes and bioactive small molecules. The copper 2,3-diketo-L-gulonic acid complex is formed by coordination of 2,3-diketo-L-gulonic acid and copper ions. The complex can have an insertion type interaction with DNA and mediate DNA damage, and realizes multiple bioactivities of antibacteriophage, antibacterial and antitumor by virtue of the core action mechanism, has multiple application scenes, remarkable effects and a wide application range.

The invention belongs to the technical field of agricultural hydrogel development, and particularly relates to hydrogel for slowly releasing plant biostimulant as well as a preparation method and application of the hydrogel. The hydrogel is obtained by crosslinking the polyglutamic acid and the 2-keto-L-gulonic acid fermentation liquor. The hydrogel disclosed by the invention is relatively high in mechanical strength, has relatively good adsorption performance on soil moisture and shows good water storage and retention capacity, so that the use amount of irrigation water is effectively reduced; besides, the hydrogel can continuously release glutamic acid, 2-keto-L-gulonic acid, D-gulonic acid-gamma-lactone, citric acid and other micromolecular organic matters with the plant biostimulant function in the soil microenvironment, the content of quick-acting organic nutrients in soil is effectively increased while the water retention effect is achieved, the microbial activity is enhanced, and the soil remediation effect is improved. The soil conditioner can improve crop rhizosphere soil and strengthen the synergistic effect of water and fertilizer, thereby effectively promoting crop growth, yield increase and vitamin C accumulation, and has good application value in the fields of agriculture and environment.

This invention belongs to the fields of genetic engineering and bioengineering technology, specifically disclosing a method for synthesizing 2-keto-L-gulonic acid (2-KLG) using Bacillus as a substrate strain. This invention achieves single-strain, one-step fermentation for 2-KLG production using Bacillus as a substrate strain by introducing and expressing sorbitol dehydrogenase and sorbitol dehydrogenase with NAD(P)H or FADH as coenzymes in Bacillus. This invention provides a novel technical route for the microbial fermentation production of 2-KLG, with the potential to replace the traditional two-step fermentation process. It also provides reference and inspiration for the exploration and optimization of biosynthetic pathways for other important compounds.

The invention relates to the technical field of microbial breeding and fermentation engineering, in particular to an L-sorbose-resistant associated bacterium Y31 and application thereof in fermentation of 2-keto-L-gulonic acid. The companion fungus Y31 is classified and named as bacillus endophyticus, and the preservation registration number of the companion fungus Y31 is CCTCC (China Center For Type Culture Collection) NO: M20251995. The strain can normally grow in a culture medium with high L-sorbose content, and can significantly improve the yield of 2-keto-L-gulonic acid (2-KLG) when mixed with acid-producing bacteria for fermentation, shorten the fermentation period and improve the production efficiency of the vitamin C precursor 2-keto-L-gulonic acid.

The present application belongs to the field of agricultural planting, and particularly relates to a microbial synergistic plant planting method in heavy metal contaminated soil. The planting method comprises the following steps: planting plants mainly in the form of crops in the heavy metal contaminated soil to be repaired; applying 2-keto-L-gulonic acid mother liquor, plant growth promoting bacteria and plant biological stimulants to the soil around the roots and / or the leaves of the plants in the vegetative growth stage of the plants; and applying the gulonic acid mother liquor and endophytic bacillus in the reproductive growth stage of the plants. In the vegetative growth stage of the crops, the 2-keto-L-gulonic acid mother liquor, the plant growth promoting bacteria and the plant biological stimulants are applied, and in the reproductive growth stage, the 2-keto-L-gulonic acid mother liquor and the endophytic bacillus are applied, which not only greatly improves the heavy metal enrichment efficiency of the roots, stems and leaves of the crops, but also greatly reduces the heavy metal concentration of the seeds of the crops and meets the national feed or food standards.

The invention belongs to the field of agricultural planting, and particularly relates to a method for planting plants in heavy metal contaminated soil through microorganism synergy. The planting method comprises the following steps that plants mainly including crops are planted in the heavy metal contaminated soil to be repaired; 2-keto-L-gulonic acid mother liquor, plant growth-promoting bacteria and plant biostimulant are applied to soil around roots and / or leaf surfaces of the plants in the vegetative growth stage of the plants; and applying the gulonic acid mother liquor and the plant endophytic bacillus to soil and / or leaves around roots of the plants in the reproductive growth stage of the plants. According to the method, the 2-keto-L-gulonic acid mother liquor, the plant growth-promoting bacteria and the plant biostimulant are applied in the vegetative growth stage of crops, and the 2-keto-L-gulonic acid mother liquor and the plant endophytic bacillus are applied in the reproductive growth stage, so that the heavy metal enrichment efficiency of roots, stems and leaves of the crops is greatly improved; and meanwhile, the heavy metal concentration of crop seeds is greatly reduced, and the national feed or food standard is met.

The application belongs to the technical field of agricultural hydrogel development, and particularly relates to a slow-release plant biological stimulant hydrogel and a preparation method and application thereof. The hydrogel is obtained by cross-linking polyglutamic acid and 2-keto-L-gulonic acid fermentation liquor. The hydrogel has high mechanical strength and good soil moisture adsorption performance, and shows good water storage and retention capacity, thereby effectively reducing the irrigation water consumption. In addition, the hydrogel can continuously release small-molecule organic matters with plant biological stimulant function such as glutamic acid, 2-keto-L-gulonic acid, D-gulonic acid-gamma-lactone and citric acid in the soil microenvironment, effectively improves the content of the soil available organic nutrients, enhances the microbial activity, improves the crop rhizosphere soil, strengthens the water and fertilizer synergistic effect, thereby effectively promotes the growth of crops, improves the yield and accumulates vitamin C, and has good application value in the fields of agriculture and environment.