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Preparation technology for 2-keto -L-gulonic acid ferment strain

A technology of keto-based cologne and fermentation strains, applied in the direction of fermentation, microbial-based methods, bacteria, etc., can solve the problems of long fermentation period, low strain density, increased energy consumption and cost, etc., and shorten fermentation cycle, increase the activity of the strain, and increase the effect of the density of the strain

Active Publication Date: 2009-11-11
DSM JIANGSHAN PHARMACEUTICAL (JIANGSU) CO LTD
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  • Summary
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AI Technical Summary

Problems solved by technology

In the known production technology of 2-keto-L-gulonic acid fermentation strains: when making slant strains, large and small bacteria are mixed together for mixed culture; in the liquid seed medium formula, the carbon source mainly uses sorbose and glucose, and adopts The bacterial classification that this method makes, small bacterium ratio is on the low side, and bacterial classification density is low, causes the fermentation cycle in the actual production to be long, and fermentation conversion rate is low, thus affects the output of 2-keto-L-gulonic acid, increases energy consumption and cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] First inoculate the small bacteria individually on the slant medium and cultivate them for 24 hours, then inoculate the large bacteria, and continue to cultivate for 28 hours. L fructose was used as the main carbon source, and the number of bacteria in the inoculum solution was 5.5×10 8 Individuals / ml, inoculated to 1m by expanding culture 3 In the fermenter, the fermentation ended for 46 hours, the 2-keto-L-gulonic acid content at the end point of the fermentation broth was 101.63mg / ml, and the fermentation conversion rate was 92.56%.

Embodiment 2

[0009] First inoculate the small bacteria separately on the slant medium and cultivate them for 20 hours, then inoculate the large bacteria, and continue to cultivate for 32 hours. L sucrose was used as the main carbon source, and the number of bacteria in the inoculum solution was 1.9×10 9 Individuals / ml, inoculated to 50m by expanding culture 3 In the fermenter, the fermentation ended for 45 hours, the 2-keto-L-gulonic acid content at the end point of the fermentation liquid was 104.86mg / ml, and the fermentation conversion rate was 92.91%.

Embodiment 3

[0011] First inoculate the small bacteria separately on the slant medium and cultivate them for 32 hours, then inoculate the large bacteria and continue to cultivate for 34 hours. L of sucrose was used as the main carbon source, and the number of bacteria in the inoculum solution was 8.3×10 9 Individuals / ml, inoculated to 100m by expanding culture 3 In the fermenter, the fermentation ended for 41 hours, the 2-keto-L-gulonic acid content at the end point of the fermentation broth was 104.81mg / ml, and the fermentation conversion rate was 92.81%.

[0012] The average fermentation period of this technology is shortened by 15%, the fermentation conversion rate is increased by 2 percentage points, the utilization rate of equipment is greatly improved, and the cost is also greatly reduced.

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Abstract

The invention discloses a preparation technology for 2-keto-L-gulonic acid ferment strain, adopting large and small bacteria for mixed culture, wherein, the small bacteria is ordinary ketogenic gulonic acid bacteria, and the large bacteria is Bacillus megatherium. The invention comprises the following steps: firstly, inoculating the small bacteria onto a slant culture medium to cultivate for 16 to 48 hours separately; then, inoculating the large bacteria and continuing to cultivate for 24 to 72 hours with the quantity ratio between the large bacteria and the small bacteria being 1:(20-40); taking sucrose with the concentration of 5-30g / L or fructose with the concentration of 5-20g / L as the main carbon source in the culture medium; introducing the seed mixture of large and small bacteria into the culture medium for cultivation and controlling the bacteria count of seeding liquid to 10-10 / ml. The invention changes the proportion between large bacteria and small bacteria, increases the strain density, and improves the strain vitality, thus shortening the fermentation period; the invention is further characterized by unchanging strain, easy operation and widespread use.

Description

technical field [0001] The invention relates to a preparation method of vitamin C, in particular to a preparation process of 2-keto-L-gulonic acid fermentation strain. Background technique [0002] At present, my country's vitamin C manufacturers all adopt two-step fermentation production technology. Its main feature is that the second-step fermentation process is completed by the mixed culture of two kinds of bacteria. Among them, the common ketogenic cologne converts sorbose into 2-keto-L-gulonic acid, commonly known as small bacteria, and the other bacteria is Bacillus megaterium, which acts as the companion bacteria of small bacteria to promote the growth and acid production of small bacteria. Commonly known as bacteria. Both bacteria work together to convert sorbose to 2-keto-L-gulonic acid. In the known production technology of 2-keto-L-gulonic acid fermentation strains: when making slant strains, large and small bacteria are mixed together for mixed culture; in the...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P39/00C12P7/60C12R1/11
Inventor 黄建明胡少斌陈红袁训吴新莉朱涛
Owner DSM JIANGSHAN PHARMACEUTICAL (JIANGSU) CO LTD
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