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Process for one-step fermentation production of gulonic acid (VC precursor) from glucose

A technology of glucose and gulonic acid, applied in the field of preparation technology of vitamin C, can solve the problems of no profit and high manufacturing cost, and achieve the effects of simple manufacturing method and reduced production cost

Inactive Publication Date: 2007-05-23
葛忠良 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] Nevertheless, the manufacturing cost of this method is still very high relative to the international market price of VC, especially for sorbitol, which requires 1.7 tons of sorbitol to produce 1 ton of vitamin C, so that almost no manufacturers adopt this method to produce VC. profit

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Specifically follow the steps below:

[0025] 1) Select an appropriate amount of Xanthomonas campestris 60 Co irradiation 200Gy, irradiation for 24 minutes;

[0026] 2) The irradiated Xanthomonas campestris was first cultured with a plate medium for 2 to 5 days at 35°C, and then a better colony was selected, which met the requirements of smooth and smooth colonies, and then inoculated in a medium containing 50ml of culture medium. Cultivate in a Erlenmeyer flask, and the culture solution contains 35g of glucose, 5g of yeast extract, and K 2 HSO 4 8g and MgSO 4 ·7H 2 O 0.6g aqueous solution;

[0027] 3) adding the mutated and cultured monocysts to the glucose-containing medium and culturing on a shaker at 40° C., so as to prepare guronic acid.

[0028] The conversion rate of the gulonic acid prepared by the method can reach 35%.

Embodiment 2

[0030] Specifically follow the steps below:

[0031] 1) Select an appropriate amount of Xanthomonas campestris 60 Co irradiation 3200Gy, irradiation 1.5 minutes;

[0032] 2) After the irradiated Xanthomonas campestris was cultured on a plate medium for 2 to 5 days at 30°C, a better colony was selected, which met the requirements of smooth and flat colonies, and inoculated on a medium containing 50ml of culture medium. Cultivate in the Erlenmeyer flask, and the culture medium contains glucose 40g, yeast extract 3g, K 2 HSO 4 5g and MgSO 4 ·7H 2 O 0.6g aqueous solution;

[0033] 3) adding the mutagenized and cultured monocysts to the glucose-containing medium and culturing on a shaker at 30° C., so as to prepare guronic acid.

[0034] The conversion rate of the gulonic acid prepared by the method can reach 40%.

Embodiment 3

[0036] Specifically follow the steps below:

[0037] 1) Select an appropriate amount of Xanthomonas campestris 60 Co was irradiated with 1800Gy for 15 minutes;

[0038] 2) After the irradiated Xanthomonas campestris was cultured with a plate medium for 2 to 5 days at 25°C, a better colony was selected, which met the requirements of smooth and smooth colonies, and inoculated on a medium containing 50ml of culture medium. Cultivate in an Erlenmeyer flask, and the culture solution contains 45g of glucose, 1g of yeast extract, and K 2 HSO 4 2g and MgSO 4 ·7H 2 O 0.6g aqueous solution;

[0039] 3) adding the mutagenized and cultured monocysts to the glucose-containing medium and culturing on a shaker at 37° C., so as to prepare guronic acid.

[0040] The conversion rate of the gulonic acid prepared by the method can reach 50%.

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PUM

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Abstract

The invention discloses a method for producing gulonic acid (VC precursor). The method comprises the steps as follows: 1) inducing proper amount of Xanthomonas campestris with ray, 2) culturing on a plate at 25-35DEG C for 2-5d, selecting the fine colony which satisfies the requirement of smooth colony, inoculating in a conical flask with culture fluid for culturing, 3) adding into the medium containing glucose for culturing to producegulonic acid. The invention realizes direct production ofgulonic acid from glucose to produce VC, with a converting ratio of 35-50%, has the advantages of simple production, reduces the production cost greatly.

Description

technical field [0001] The invention relates to a preparation process of vitamin C. Background technique [0002] Vitamin C (referred to as VC) is one of the vitamins that the human body cannot synthesize by itself. It participates in various metabolic processes in the body, can reduce the fragility of capillaries, and increase the body's resistance. It is used to prevent scurvy, and can also be used as an adjuvant treatment for various chronic infectious diseases and purpura. It can prevent the production of carcinogen nitrite amines. VC is an excellent antioxidant that keeps food fresh. It is widely used in the food, beverage and feed industries. The demand continues to increase with the improvement of people's living standards. In 2005, the global demand for vitamin C was more than 120,000 tons . The production and preparation of vitamin C mainly include the following types: [0003] 1) Reyes method [0004] Vitamin C was extracted from citric acid as early as 1910-1...

Claims

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Application Information

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IPC IPC(8): C12P7/60C12R1/64
Inventor 葛忠良叶剑雄刘秀珍魏文廖杰李娅孙建中马成禹李莉马松江王军
Owner 葛忠良
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