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Method for separating solution rich in gulonic acid and vitamin C by ion exclusion chromatography

A technology of ion exclusion chromatography and gulonic acid, which is applied in the field of separation by ion exclusion chromatography, can solve the problems of high resin synthesis requirements, unfavorable industrial production, and difficulty in continuing crystallization, and achieve the effects of reducing production costs and increasing yields

Active Publication Date: 2009-12-30
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If separation and purification are not carried out, it will cause difficulties to continue crystallization and reduce the yield of vitamin C
[0004] CN1733747A discloses a specially synthesized chelating resin to extract gulong acid and vitamin C in Vc mother liquor, which has high requirements for resin synthesis and high extraction cost, which is not conducive to industrial production

Method used

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  • Method for separating solution rich in gulonic acid and vitamin C by ion exclusion chromatography
  • Method for separating solution rich in gulonic acid and vitamin C by ion exclusion chromatography
  • Method for separating solution rich in gulonic acid and vitamin C by ion exclusion chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In the moving bed chromatographic system (produced by L100-139 U.S. CALGON company) with 20 resin columns, its resin column volume is 900ml / root, pack 800ml macroporous strong acid cation chromatographic separation resin by every resin column, resin grain Diameter 0.45mm. For column layout, see figure 1 shown.

[0022] The pH of the material rich in gulonic acid and vitamin C solution in the raw material is 4.0, and the components are 23.3% of gulonic acid radical, 16.5% of Vc and 8.6% of sorbose. Under the condition that the operating temperature of the moving bed is 45°C and the walking time is 900S, the chromatographic separation parameters are as follows:

[0023] Material name

Pure water (L / h)

Raw material(L / h)

Gulonic acid-rich components (L / h)

Vc-rich component (L / h)

flow

2.2

0.48

1.6

0.6

[0024] After three cycles, collection of the separated fractions was started. Its composition is as follows...

Embodiment 2

[0030] In the simulated moving bed chromatographic system with 12 resin columns, the volume of the resin column is 1000ml / piece, and the volume of each resin column is 900ml, and the gel-type strong acid cation chromatography separation resin is loaded, and the particle size of the resin is 0.30±0.05mm. For column layout, see figure 2 shown.

[0031] The pH of the material rich in gulonic acid and vitamin C solution is 1.0, and the components are 23.3% of gulonic acid, 16.5% of Vc and 8.6% of sorbose. Under the condition that the operating temperature of the moving bed is 30°C and the walking time is 1500S, the chromatographic separation parameters are as follows:

[0032] Material name

Pure water (L / h)

Raw material(L / h)

Gulonic acid-rich components (L / h)

Vc-rich component (L / h)

flow

2.76

0.36

1.68

0.72

[0033] After three cycles, start to collect the separated components, the composition of which is as follow...

Embodiment 3

[0039] In the simulated moving bed chromatographic system with 12 resin columns, the volume of the resin column is 1000ml / piece, and the volume of each resin column is 900ml, and the gel-type strong acid cation chromatography separation resin is loaded, and the resin particle size is 0.40±0.10mm. For column layout, see figure 2 shown.

[0040] The pH of the material rich in gulonic acid and vitamin C solution is 3.0, and the components are 23.3% of gulonic acid, 16.5% of Vc and 8.6% of sorbose. Under the condition that the operating temperature of the moving bed is 50°C and the walking time is 1200S, the chromatographic separation parameters are as follows:

[0041] Material name

Pure water (L / h)

Raw material(L / h)

Gulonic acid-rich components (L / h)

Vc-rich component (L / h)

flow

3.45

0.45

2.1

0.9

[0042] After three cycles, start to collect the separated components, the composition of which is as follows:

[00...

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Abstract

The invention provides a method for separating solution rich in gulonic acid and vitamin C by ion exclusion chromatography, which mainly uses different repulsion forces of strong-acidic group of strong-acidic ion exchange resin on the gulonic acid and the vitamin C to separate the gulonic acid and the vitamin C. The method is realized through a moving bed system or a simulated moving bed system, can improve the yield of vitamin C (Vc) products, reduce production cost, and ensure the yield of gulonic acid and vitamin C over 90 percent.

Description

technical field [0001] The invention relates to a separation method using ion exclusion chromatography, in particular to a method for separating a solution rich in gulonic acid and vitamin C or sodium gulonic acid and vitamin sodium by using ion exclusion chromatography. Background technique [0002] Vitamin C is widely used in medicine, food, feed, cosmetics and many other fields, and has broad market prospects. In order to improve the quality and yield of vitamin C, the production process has been improved at home and abroad. [0003] At present, the process of industrial production of vitamin C generally adopts the "two-step fermentation" process. The "two-step fermentation method" production of vitamin C is divided into three steps: fermentation, extraction and transformation. Among them, the mother liquor obtained from the vitamin C coarse crystallization process is rich in substances such as a large amount of vitamin C, gulong acid and sorbose. If separation and pur...

Claims

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Application Information

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IPC IPC(8): C07D307/62C07C59/105C07C51/47B01D15/04B01D15/08
Inventor 李荣杰尚海涛潘声龙付松
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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