Gulonic acid adsorption separation method utilizing hyper-crosslinked resin

An ultra-high cross-linked resin, adsorption and separation technology, applied in chemical instruments and methods, separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of many unit operations, pollution, and large consumption of ion exchange resins, etc., to achieve Effects of avoiding environmental pollution, large adsorption capacity, and reducing waste water discharge

Active Publication Date: 2015-04-22
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology helps identify ways to improve how we filter materials like glucuronolactone (GL), citric acid, fumarate salt, etc., while still maintaining their effectiveness over other substances that are similar). By doing this, these technologies help us make better products from raw materials without generating harmful emissions.

Problems solved by technology

Technologies described involve separating different types of compounds called glucose-6-hexanoyl-4-oxygenase-2(GI2) into pure enzyme without any unwanted side effects like excessive pH values caused by hydrochloride salts. Additionally, current techniques require expensive equipment and complicated processes with multiple steps involving several purification stages.

Method used

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  • Gulonic acid adsorption separation method utilizing hyper-crosslinked resin
  • Gulonic acid adsorption separation method utilizing hyper-crosslinked resin
  • Gulonic acid adsorption separation method utilizing hyper-crosslinked resin

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Embodiment 1: Obtaining of gulonic acid feed solution.

[0047] The percentage content of gulonic acid in the fermented liquid obtained by Bacillus megaterium and Ketogulonicigenium vulgare fermentation is 35% (w / w), and its fermentation process is as follows: Prepare plate culture medium: 8.0% L-sorbose, 1.0% corn steep liquor, 0.2 % urea, 0.1% KH 2 PO 4 , 0.01% MgSO 4 ·7H 2 O, 0.5% NaCl, 1.5% agar was sterilized at 121°C for 15min. The pH of the fermentation broth was adjusted to about 7.0. Add half of the nutrients initially, and then gradually add them later. Cultivate in a Bugbox anaerobic tank at 37° C. for 72 h with a stirring speed of 300 rpm. Gulong acid ~ 35g / L in the obtained fermentation broth, residual L-sorbose<1g / L.

[0048] The above-mentioned Bacillus megaterium and Ketogulonicigenium vulgare can be replaced by other bacillus to realize the fermented liquid containing gulonic acid prepared by fermentation of different strains.

[0049] The ferme...

Embodiment 2

[0050] Example 2: Preparation of ultra-high cross-linked resin HD-01.

[0051] In a 2000mL three-necked flask, add 200g of dry chloromethylated polystyrene resin, add 1500g of nitrobenzene, stir and mix well and fully swell at room temperature for 2 hours, add 12g of aluminum trichloride and 40g of divinylbenzene, and stir at room temperature After reacting for 1 hour, the temperature was raised to 95° C. at a rate of 1° C. / min to carry out the cross-linking reaction. After reacting for 8 h, 300 mL of ethanol was added to stop the reaction, and cooled to room temperature. Suction filtration, wash off the residual solvent and catalyst with ethanol, and vacuum dry to obtain the weak polar ultra-high cross-linked resin HD-01. The obtained product is in the shape of dark brown round particles, with an average particle size of 0.42-0.56mm, a water content of 43wt%, a pore size of 1.92nm, a porosity of 30-40%, and a wet density of 1.09g / cm 3 , the average specific surface area is ...

Embodiment 3

[0052] Embodiment 3: Pretreatment of ultra-high cross-linking resin HD-01.

[0053] Rinse the resin with 2BV 1mol / L HCl solution at a flow rate of 1BV / h, and then wash with water until neutral; wash the resin with 2BV 1mol / L NaOH solution at a flow rate of 1BV / h, and then wash with water until neutral; finally use 2BV Rinse the resin with ethanol at a flow rate of 2BV / h, wash away the ethanol with deionized water, and set aside.

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Abstract

The invention discloses a gulonic acid adsorption separation method utilizing hyper-crosslinked resin. According to the gulonic acid adsorption separation method utilizing the hyper-crosslinked resin, a preprocessed gulonic acid fermentation broth is subjected to the processes of absorption, impurity washing and desorption through an adsorbing column filled with the hyper-crosslinked resin, and finally a gulonic acid solution is obtained. The hyper-crosslinked resin is prepared through a Friedel-Crafts cross-linking reaction and with polystyrene-divinyl benzene as a framework, and is weak in polarity and good in hydrophilia; the average grain diameter is 0.42-0.56mm, the water content is 43wt%, the bore diameter is 1.92nm, the porosity is 30-40%, the wet density 1.09g/cm3, the average specific surface area is 1645.5m2/g, the average pore volume is 0.732cm3/g, and a functional group is a carbanyl group. The adsorption medium adopted by the method has the advantages of being large in adsorption capacity for the gulonic acid, good in adsorptive selectivity, mild in desorption condition and long in service cycle, enabling regeneration to be easy and the like.

Description

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Claims

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Application Information

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Owner NANJING UNIV OF TECH
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