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A kind of medium for liquid submerged fermentation of Cordyceps militaris and using method thereof

A liquid submerged fermentation and Cordyceps militaris technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of overflow and dissolution of bacterial contents, bacterial rupture, etc., and increase fermentation yield , the effect of reducing production costs

Inactive Publication Date: 2017-06-20
SHENYANG YUANKANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the late stage of vitamin C biological fermentation, there is a phenomenon that the bacteria burst, and the contents of the bacteria overflow and dissolve in the fermentation liquid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The invention provides a medium for the liquid submerged fermentation of Cordyceps militaris, which consists of the following raw materials: 20g of sucrose, 2g of corn steep liquor, 8g of mash protein dregs, 2g of peptone, 1.5g of beef extract, MgSO 4 •7H 2 O 0.8g, KH 2 PO 4 0.5g, vitamin B12mg, water 1000mL.

[0028] The invention provides a method for using a culture medium for liquid submerged fermentation of Cordyceps militaris, and the specific process parameters are as follows.

[0029] The medium loading capacity in the fermenter was 65%, and then sterilized at a temperature of 124°C for 40 minutes, and the liquid Cordyceps militaris was inoculated when the temperature of the medium dropped to 25°C.

[0030] During the liquid fermentation process, the temperature in the fermenter is controlled at 22°C, and the ventilation in the fermenter is controlled at 1:0.3m 3 / m 3 / min, the stirring speed was controlled at 110rpm / min, the pH value in the early stage of...

Embodiment 2

[0033] The invention provides a culture medium for the liquid submerged fermentation of Cordyceps militaris, which is composed of the following raw materials: 20 g of sucrose, 2 g of corn steep liquor, 20 g of protein dregs of mash, 0.5 g of beef extract, MgSO 4 •7H 2 O 0.8g, KH 2 PO 4 0.5g, vitamin B1 2mg, water 1000mL.

[0034] The invention provides a method for using a culture medium for liquid submerged fermentation of Cordyceps militaris, and the specific process parameters are as follows.

[0035] The medium loading capacity in the fermenter was 60%, and then sterilized at a temperature of 122°C for 40 minutes, and the liquid Cordyceps militaris was inoculated when the temperature of the medium dropped to 25°C.

[0036] During the liquid fermentation process, the temperature in the fermenter is controlled at 22°C, and the ventilation in the fermenter is controlled at 1:0.3m 3 / m 3 / min, the stirring speed was controlled at 110rpm / min, the pH value in the early sta...

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Abstract

The invention discloses a medium for cordyceps militaris liquid deep fermentation. The medium comprises 10-30g of cane sugar, 1-5g of corn steep liquor, 4-100g of fermenting mash protein residue, 0-4g of peptone, 0-4g of beef extract, 0.5-2.0g of MgSO4.7H2O, 0.2-1.0g of KH2PO4, 1-5mg of vitamin B1 and 1000ml of water. The medium contains waste fermenting mash protein residue obtained by vitamin C fermentation, the fermenting mash protein residue contains mycoprotein of bacillus megatherium and ketogulonigenium vulgare, and the mycoprotein is similar to silkworm pupa protein and thus the mycoprotein can completely or partially replace beef extract and peptone in the medium. The medium can improve a cordyceps militaris mycelium fermentation yield, realize resource utilization of the waste fermenting mash protein residue obtained by a vitamin C fermentation industry and effectively reduce a production cost. The invention also provides a use method of the medium for cordyceps militaris liquid deep fermentation.

Description

technical field [0001] The invention relates to the field of edible fungus cultivation, in particular to a culture medium for liquid submerged fermentation of Cordyceps militaris and a use method thereof. Background technique [0002] The scientific name of Cordyceps militaris is Cordyceps militaris ( Cordyceps militaris ), it and Qinghai Cordyceps belong to the fungal phylum Ascomycotina genus Cordyceps, therefore, it is called the "brother" of Cordyceps by industry experts. The sexual stages of the life cycle of Cordyceps militaris include mycelium growth and fruiting body growth. Insect larvae or pupae of Lepidoptera, Coleoptera, and Diptera are infected with spores of Paecilomyces spores. When the environmental conditions are suitable, the spores germinate to form mycelium, and the mycelium absorbs the nutrients in the insect (pupa) as its Materials and energy for growth and development. After the mycelium decomposes the various tissues and organs in the worm (pupa), ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 徐慧佟庆辉杨伟超孔双王楠周浩
Owner SHENYANG YUANKANG BIOTECH
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