Mutation screening method of ketogulonigenium vulgare

Abstract

The invention provides a mutation screening method of ketogulonigenium vulgare. The ketogulonigenium vulgare in the vitamin C fermentation production is used as an original strain, low-temperature normal-pressure plasma mutation equipment is adopted for mutation, a solid medium is coated with the ketogulonigenium vulgare, single colonies evenly grow out, single-bacterium multiplication and enlarged culture continue to be carried out, and target bacterial strains are screened out through a companion fungus shaking bottle. By means of the technology, the mutation screening parallelity of the ketogulonigenium vulgare is improved, and the probability of obtaining efficient bacterial strains is increased.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, in particular to the technical field of mutation breeding. Specifically, the present invention relates to a method for mutagenesis screening of the vitamin C producing strain Vulgaris ketogenic cologne. The invention also provides a special culture medium for screening common ketogenic cologronic acid bacteria, which can realize the parallel cultivation of a single colony of common ketogenic cologronic acid bacteria. Background technique [0002] Vitamin C is a water-soluble vitamin necessary for the human body. It has antioxidant properties and improves the body's immunity. It is widely used in medicine, food, cosmetics and other fields. [0003] At present, vitamin C is mainly produced by a two-step fermentation process. Ketogulonigenium vulgare is combined with Bacillus megaterium to convert the substrate L-sorbose into gulonic acid (ie 2-keto-L-gulgare). , In this process, Ba...

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Problems solved by technology

[0005] Ordinary ketogenic colonacid bacteria have weak single-bacteria growth ability, especially for strains that have undergone mutagenesis treatment, it is difficult to achieve single-colony growth under ordinary culture conditions

Although the Chinese patent application (application number: 201210308418.X, published on January 2, 2013) provides a single colony growth method on a flat plate, it still needs to be realized by overlapping associated bacteria, and the overlapping method is to separate points on the flat plate Then, the parallel growth of each single colony of common ketogenic cologronic acid bacteria cannot be achieved during the cultivation process, and the large colony of the accompanying bacteria will cover the mutagenized strains, so that the mutagenized strains will be missed, and all of them cannot be expanded for cultivation. However, the method of overlapping associated bacteria is still used. In this process, the human factors of the operator are greatly affected, causing the excessive size of associated bacteria to affect the proliferation and culture of each single colony, which in turn affects the stability of the screening results.

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