22 results about "Associated bacteria" patented technology

The invention relates to the technical field of resources and environment, in particular to a method for pretreating lignocellulose by using a rumen microorganism dry method. The method comprises the following steps: crushing lignocellulose to 1-5cm, and filling the crushed lignocellulose into a sealed space; spraying microorganisms under an anaerobic condition according to a mass ratio of rumen microorganisms to lignocellulose volatile components of (0.015-0.03): 1, and controlling the water content of the system to be 30-60%; the pretreatment temperature is regulated and controlled to be 35-42 DEG C, the pH is 6.0-7.5, the pretreatment time is 10-14 days, so that the total abundance of the functional microorganisms Pseudocidovorax, Hallella and Prevotella is greater than or equal to 55%, and the three types of microorganisms respectively form strong interaction with specific companion bacteria; when the relative crystallinity of the lignocellulose is reduced by more than 15% and the volatile component is reduced by 10%, finishing the pretreatment; and performing process regulation and control according to the crystallinity and the reducing sugar yield when the pretreatment is performed for 10 days. The dry process is adopted, the bionic principle and the solid-state fermentation technology are combined, all-component gradient utilization of lignocellulose is achieved, a green solution is provided for industrial and high-value conversion of lignocellulose, and remarkable economic, social and environment-friendly benefits are achieved.

The invention relates to a dental and / or oral care product comprising between 0.0039% and 0.0078% by weight of nisin establishing and / or maintaining a pH of at least 4.5 in situ. Although said rather low nisin concentration does not provide an anti-bacterial effect, it is surprisingly and advantageously capable of maintaining stable pH values of at least 4.5, thereby preventing destruction of a subject's teeth enamel based on enamel demineralization effects at pH below 4.5, and said low nisin concentration advantageously further avoids dysbacteriosis, i.e. the oral microflora remains balanced, thus not leading to an increase of e.g. periodontitis associated bacteria.

The invention discloses a method for removing companion bacteria in tissue culture subculture multiplication culture of clerodendrum trichotomum and application of the companion bacteria in tissue culture subculture multiplication culture of clerodendrum trichotomum. The method comprises the following steps: carrying out pretreatment and disinfection treatment on explants of stem segments with buds of clerodendrum trichotomum, and establishing a sterile system; inoculating the explants to an induction medium, culturing for 14 days to form calluses, transferring the calluses to a redifferentiation medium, and culturing for 7 days to form adventitious buds; the adventitious buds are transferred to a proliferation culture medium, companion fungus pollution occurs during proliferation culture, a sterile gentamicin sulfate solution with the concentration of 800 mg / L is adopted for treatment, the companion fungus clearance rate reaches 100%, the clerodendrum trichotomum sterile seedlings can normally grow, plants are robust and free of yellowing and vitrification phenomena, and the proliferation coefficient reaches up to 16 (24). According to the method, the problem that in a traditional mode, the reproduction period is greatly prolonged due to pollution, namely waste reproduction, is effectively solved, waste of valuable and rare plant materials is reduced, and the tissue culture seedling culture success rate is greatly increased.

The present invention relates to a method for reducing WST-8 to formazan based on NAD(P)H for determining the susceptibility of certain bacterial isolates to infection by certain bacteriophages, thereby allowing direct observation of the results measured as cell death of the target bacteria. The reduction of tetrazolium salts to formazan by NAD(P)H is used to determine the metabolic activity of cells and as an indicator of cell viability; whereas WST-8 is used to qualitatively measure the bacteriophage killing activity of the target bacteria. The present invention thus belongs to the field of medical biology, in particular to in vitro laboratory test methods.

This invention relates to the field of gene detection technology, specifically to a primer set and its application, and a training method for a bacterial vaginosis diagnostic model. The primer set, used for diagnosing bacterial vaginosis, includes specific primers for specifically amplifying target regions of the following pathogens: *Atopobacterium vaginalis*, *Gardnerella vaginalis*, *Cyclophorus 1*, *Cyclophorus 2*, *Bacterial Vaginosis-Associated Bacteria 2*, *Animal Curvularia clarithii*, *Animal Curvularia emblica*, *Prevotella 2*, *Lactobacillus curvularia*, *Lactobacillus indolentus*, *Lactobacillus gasseri*, and *Lactobacillus janniae*. This invention also provides the application of this primer set and a training method for a bacterial vaginosis diagnostic model. Using this primer set, high-throughput sequencing can be performed on test samples, simultaneously detecting 12 target pathogens, and further constructing a bacterial vaginosis diagnostic model, which can be used for the clinical diagnosis of bacterial vaginosis with good specificity and sensitivity.

The present invention provides a composition comprising fosfomycin for reducing or removing bacterial promoting factors of colorectal cancer from colorectal adenomas, polyps, and adenocarcinomas, as well as from associated bacterial biofilms, by local administration into the intestinal lumen.

Oral composition comprising glutamate, or glutamine, solvate or hydrate thereof, for use in the treatment and / or prevention of caries, wherein the composition is orally administered and increases the pH of the saliva, reducing the risk of caries development. Said oral composition increases the amount of health-associated bacteria and / or decreases the amount of disease-associated bacteria and is useful for the treatment and / or prevention of oral diseases, such as periodontal diseases, halitosis and diseases caused by Candida. Method to determine the response of a mammal to the treatment with glutamate, or with glutamine, solvate or hydrate thereof, comprising determining in saliva the amount of health-associated or disease-associated bacteria or a dysbiosis index, and identifying the mammal as responsive or non-responsive to the treatment.

The invention discloses application of Pixantrone in preparation of a medicine for treating bacterial infection, relates to the technical field of medicines, and finds that the Pixantrone in a nitrided anthraquinone derivative can be used for preparing a medicine for treating bacterial infection by inhibiting the activity of histidine dehydrogenase (HisD) on the basis of an emerging anti-infection strategy of anti-virulence therapy (anti-virulence therapy), so that the Pixantrone in the nitrided anthraquinone derivative can be used for preparing a medicine for treating bacterial infection, and the application of the Pixantrone in the nitrided anthraquinone derivative can be used for preparing a medicine for treating bacterial infection. Compared with the prior art, the method has the advantages that the hemolytic activity (the inhibition rate reaches 50% or above) and the biofilm forming ability of related bacteria (such as staphylococcus aureus) can be obviously reduced, the virulence gene expression is reduced, an important basis is provided for the anti-virulence treatment of multi-drug-resistant bacteria (such as staphylococcus aureus) infection, the drug resistance risk can be reduced, the host immune clearance ability can be enhanced, and the application prospect is wide. And a plurality of problems existing in a traditional antibiotic treatment scheme of bacteria are solved.

The invention discloses a traditional Chinese medicine composition with a bacteriostatic effect as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The antibacterial composition is prepared from the following components in parts by weight: 1 part of cortex phellodendri and 16 parts of ginger, has an excellent inhibition effect on escherichia coli and staphylococcus aureus, and can be used for preventing and treating diseases caused by related bacterial infection and killing related bacteria in the environment.

The present invention belongs to the technical field of agricultural microorganisms, and particularly relates to a growth-promoting bacterium for promoting the growth of Caulerpa lentillifera and its application. The present invention discloses a co-associated bacterium capable of promoting the growth of algae. The strain is Bacillus sp. Cl-9. The morphological characteristics of strain Cl-9 are: light pink, opaque, smooth surface, smooth edge, and circular. This co-associated bacterium not only has the function of dissolving inorganic phosphorus and can be prepared into a phosphorus solubilizer for increasing the phosphorus content in water bodies, but also can colonize on the algal thalli, significantly increase the biomass of algae, and promote the growth of the upright branches of algae. It can be further developed into a microbial inoculant or microbial fertilizer for promoting the growth of algae. The present invention provides a reference for the application and development of co-associated bacteria in promoting the growth of algae, and has broad application prospects.

The application discloses a natural product 3-methylcarbazole derivative with a general formula B and a preparation method and application thereof in the technical field of organic chemistry. S. aureus S. epidermidis The derivative has good antibacterial activity, can significantly inhibit Staphylococcus aureus, Staphylococcus epidermidis and methicillin-resistant Staphylococcus aureus (MRSA), and can be used for preparing a drug for treating related bacterial infections.​

The invention relates to the technical field of organic chemistry, and particularly discloses an isoxazole amide derivative with a structure shown in a general formula III as well as a preparation method and application of the isoxazole amide derivative. The isoxazole amide derivative disclosed by the invention shows remarkable antibacterial activity on staphylococcus aureus, escherichia coli, methicillin-resistant staphylococcus aureus (MRSA) and fluoroquinolone-resistant escherichia coli (FREC), the activity of part of compounds is superior to that of gentamicin, and the isoxazole amide derivative has broad-spectrum antibacterial potential and can be used for preparing medicines for treating related bacterial infections.

Nutrient-enriched food products and methods of producing nutrient-enriched food products using a bacterium associated with a host from a nutrient-deficient environment.

This application provides isolated antibodies, or antigen-binding fragments thereof, that specifically recognize one or more gut bacterial lipoglycans, and polynucleotides and vectors that encode for such antibodies or antigen-binding fragments. This application further provides methods of producing the antibodies or antigen-binding fragments and using the antibodies or antigen-binding fragments for treatment of diseases.

The application provides a composite microbial agent special for sewage denitrification and a carbon source preparation method thereof, and belongs to the technical field of sewage treatment. The composite microbial agent comprises a mixed bacterial solution composed of iron-oxidizing autotrophic denitrifying bacteria, heterotrophic nitrification-aerobic denitrification bacteria and iron mineral mineralization associated bacteria; the iron-oxidizing autotrophic denitrifying bacteria are Paracoccus ferrooxidans, the heterotrophic nitrification-aerobic denitrification bacteria are Pseudomonas stutzeri, and the iron mineral mineralization associated bacteria are Shewanella. The triple coupling system of autotrophic denitrification, heterotrophic denitrification and iron mineral mineralization is constructed, and the industry pain points of insufficient carbon source of low-carbon sewage, poor low-temperature denitrification, easy loss of bacterial strains and high cost of chemical carbon source are solved.

The present invention provides compositions comprising fosfomycin for reducing or eliminating bacteria that promote the development of colorectal cancer in colorectal adenomas, polyps, and adenocarcinomas, and associated bacterial biofilms, by topical administration into the intestinal lumen.

The application belongs to the technical field of blood ear culture and cultivation, and particularly discloses a blood ear strain cultivation and bottle cultivation method, which comprises the following steps: obtaining blood ear basidiospores by separating and purifying culture of basidocarps of the blood ear based on a spore shooting method; obtaining associated bacteria hyphae by separating and purifying culture of ear wood tissues of the blood ear; obtaining blood ear hyphae by pairing the blood ear basidiospores in a culture medium based on a spore pairing method; inoculating the blood ear hyphae and the associated bacteria hyphae into culture media respectively for light-avoiding activation culture to obtain cultivation mother strains respectively; loading culture material into a wide-mouth bottle, compacting, centrally inoculating a hole, covering a bottle cap with a breathable film, high-pressure sterilizing, inoculating mixed cultivation mother strains under aseptic conditions, and carrying out dark culture until the hyphae grow to fill the bottle, and then carrying out ear management to obtain blood ear basidiocarps. The method has the advantages of early ear emergence, short growth cycle, ear cultivation through replacement material, stable strain, and pure blood ear hyphae, etc.

This invention discloses a natural product 3-methylcarbazole derivative having the general formula A, its preparation method, and its application, relating to the field of organic chemistry. Its substituents R1 and R2 have the definitions given in the specification. This derivative possesses good antibacterial activity against *Escherichia coli* (E. coli). E. coli ), Pseudomonas aeruginosa ( P. aeruginosa It has a significant inhibitory effect on both fluoroquinolone-resistant Escherichia coli (FREC) and can be used to prepare drugs for treating related bacterial infections.

The invention discloses a novel application of DAT and bacteria for producing DAT. The application refers to application of DAT and bacteria generating DAT in preparation of drugs for preventing and / or treating myocardial ischemia / reperfusion injury. Prevention and / or treatment of myocardial ischemia / reperfusion injury are embodied in the following aspects: 1) heart injury after myocardial ischemia / reperfusion is relieved; 2) the apoptosis proportion of myocardial cells is reduced; 3) promotion of myocardial cell survival; 4) inhibiting cardiac fibrosis; 5) the area of cardiac infarction is reduced; and 6) inhibiting heart remodeling. Experimental results show that the pathological influence of ischemia / reperfusion injury of mice can be relieved by orally taking DAT or bacteria producing DAT, and the discovery provides a new thought and theoretical basis for development of DAT drugs and related bacteria.

A bacteria-responsive color-changing, core-shell nanofiber, comprising polyurethane (PU), a hemicyanine-based chromogenic probe localized in the core-shell nanofiber near the surface of the shell, polyvinylpyrrolidone (PVP) dopant in the shell, the hemicyanine-based chromogenic probe further comprising a labile ester linkage that is enzymatically cleavable by bacterial lipase released from clinically relevant strains of bacteria including Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA).

The invention relates to the technical field of food safety, in particular to a screening method and targeted prevention and control of microalgae high-sensitization associated bacteria. The screening method disclosed by the invention comprises the following steps: (1) extracting DNA (Deoxyribose Nucleic Acid) of microalgae, and constructing a DNA library; (2) purifying the constructed DNA library; (3) carrying out genome packaging on the Clean Data; (4) predicting and clustering the assembled genes, and constructing a non-redundant gene set; (5) performing comparison and species annotation on the non-redundant gene set, and calculating the abundance of the species; (6) screening species of which the abundance ranks the first 6; and (7) predicting the potential sensitization of the coded protein in the dominant bacteria, and selecting the dominant bacteria with the most sensitization protein as the microalgae high-sensitization associated bacteria. The obtained high-sensitization associated bacterium is brucella, and the substance for specifically removing the bacterium in a targeted manner is bacteriophage vBBruSwang.

The present invention provides compositions comprising fosfomycin for reducing or eliminating bacterial promoters of colorectal cancer from colorectal adenomas, polyps and adenocarcinomas, as well as from the associated bacterial biofilm, by local 5 administration into the bowel lumen.