168 results about "Plant nematode" patented technology

The present invention is directed to controlling plant pest infestation, and particularly plant nematode infestation, by inhibiting one or more biological functions in the plant pest. The invention discloses methods and compositions for use in controlling plant pest infestation by providing one or more different recombinant double stranded RNA molecules in the diet of the pest in order to achieve a reduction in pest infestation through suppression of pest gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, to methods for detecting cells comprising the disclosed sequences, and to methods for detecting the disclosed sequences in biological samples.

The present invention is directed to controlling plant pest infestation, and particularly plant nematode infestation, by inhibiting one or more biological functions in the plant pest. The invention discloses methods and compositions for use in controlling plant pest infestation by providing one or more different recombinant double stranded RNA molecules in the diet of the pest in order to achieve a reduction in pest infestation through suppression of pest gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, to methods for detecting cells comprising the disclosed sequences, and to methods for detecting the disclosed sequences in biological samples.

The invention relates to a method for producing a preparation with biological bacteria, which is used for preventing plant nematodes and the application of the preparation, in particular to a paecilomyces cicadae biological bacteria preparation, a method for producing the same and the application of the same to plant nematode prevention. The paecilomyces cicadae biological bacteria preparation can be the compound of paecilomyces cicadae, culture medium of the paecilomyces cicadae, auxiliary materials and a carrier and is produced by culturing paecilomyces cicadae, fermenting the paecilomyces cicadae with liquid, propagating the paecilomyces cicadae in great qualities, directly processing the paecilomyces cicadae and absorbing the paecilomyces cicadae with the auxiliary materials and the carrier. The paecilomyces cicadae biological bacteria preparation has obvious effect on plant nematode prevention, stable performance and wide application range, reaches the standard of the quality guarantee period of biological pesticide, meets the production requirements of organic food and green food, is environmental friendly, beneficial to human health and prone to large-scale industrial production and serves as broad-spectrum biological bacteria preparation for preventing plant nematodes.

The invention relates to a composition of chitin and avermectin or emamectin benzoate for insecticide and acaricide, and is characterized in that the dosage form of the compound preparation is microemulsion and the weight percentage of effective components in the compound preparation contains 0.5 to 10 percent of the chitin, 0.2 to 2 percent of the avermectin or 0.2 to 1.5 percent of the emamectin benzoate, with the rest of an auxiliary agent, a cosolvent and water. The pesticide preparation of the invention has no public pollution and can be applied to prevention of pests and mites in agricultural production, in particular to the prevention of plant nematode diseases.

The invention discloses preparation and application of a soil ecosystem nursing microorganism bacterial fertilizer. The soil ecosystem nursing microorganism bacterial fertilizer contains abundant organic matters and beneficial bacteria, especially paecilomyces lilacinus. The preparation method for the fertilizer mainly comprises the following steps: preparation of a paecilomyces lilacinus strain; enlarged cultivation of a liquid strain used for production; preparation of a medium; addition of the paecilomyces lilacinus strain for enlarged cultivation; secondary fermentation; etc. The microorganism bacterial fertilizer is applied as a base fertilizer to soil according to an amount of 100 to 200 kg / mu, can improve soil, especially soil in a greenhouse, is capable of obviously mitigating harm of a plurality of plant nematode diseases caused by root-knot nematode, soybean cyst nematode, stem eelworm and the like, and alleviates continuous cropping obstacles.

The invention discloses a bacillus thuringiensis strain which has a preservation title of bacillus thuringiensis YC-10, is preserved in China Center for Type Culture Collection and has a preservation number of CCTCC NO:M2010023. The bacillus thuringiensis strain can generate a parasporal crystal protein while forming into a gemma. A protein extraction method comprises steps of: inoculating a bacteria liquid with activated bacterial strains to a fermentation medium, culturing with oscillating, and obtaining a mixture of gammae and crystals; washing, suspending with a buffer, centrifuging to obtain a supernatant, precipitating, dissolving, removing impurities by centrifugation and dialysing overnight, wherein the dialysate contains parasporal crystals. The bacillus thuringiensis strain or the parasporal crystals produced therefrom can be effectively applied to plant nematode disease control. The strain and application thereof in the invention have advantages of high efficiency, low toxicity, ecological environment friendliness, reduction of pesticide residuals, etc.

The invention discloses a composition for preventing and treating plant nematode and virus diseases, and an application of the composition, belonging to the technical field of biopesticide. The composition takes chitosan oligosaccharide and lentinan as active ingredients and can be made into liquid or effervescent tablets. The composition can be used for separately and simultaneously preventing and treating the plant nematode and the virus diseases of plants, and has good using effect on pumpkins, winter squashes, pepper, alfalfa, lily, strawberries and gingers, thus effectively reducing the crop diseases and pest and improving the yield and the quality of crops.

The invention discloses a drug controlling plant nematodes. Effective ingredients comprise natural saponin, and the natural saponin is one or more of soapnut saponin, gleditsioside, tea saponin, saikosaponin, esculentoside, ursolic acid, vulgarsaponin and begonia yunnanensis Levl. saponin. Preferably, the drug also comprises lysobacter antibioticus, possseses relatively good killing effect on plant nematodes, nematode host cerambycidae ovum and larva and the like, and additionally, is extremely excellent in controlling effect on fruit tree gummosis. The exploited drug is environment-friendly type, is increased in controlling effect, also avoids environment harm caused by usage of a large amount of severely toxic pesticides, and possesses great reality meaning for protecting our country forest resource and ecological environment and recovering economic loss.

The invention relates to a shenqinmycin-avermectins suspoemulsion used for controlling plant nematodes and a preparation method of the suspoemulsion. The suspoemulsion comprises following components by weight: 1%-10% of shenqinmycin, 0.2%-2% of avermectins, 2%-8% of an emulsifier, 1%-5% of a dispersant, 0.1%-1% of an antisettling agent, 2-10% of an anti-freeze agent, 0.5-2% of a wetting agent and 5%-10% of a solvent, with the balance being water and the sum of the components being 100%. Compared with the prior art, the mixture provided by the invention has a contact-killing function, a stomach toxicity function, an inhibition function for normal growth of phytopathogen hyphae, and a function of causing mycelia breakage, tumidness, deformation and lysis.

The invention relates to a culturing method of streptomyces with the function of poisoning plant menatodes and a preparation method and application of the metabolin, belonging to the technical field of microbial pesticides. The streptomyces bacterial strain Snea253 is Streptomyces venezuelae, stored in China General Microbiological Culture Collection Center on January 18, 2008 (address: Datun Road, Zhaoyang District, Beijing; post code: 100101) with CGMCC No.2348. The streptomyces bacterial strain Snea253 is classified in species Streptomyces venezuelae, genus streptomyces, order actinomycetales, class actinomycetales, phylum actinomycetales. The culturing method of streptomyces with the function of poisoning plant menatode has the advantages that: through liquid fermentation culture preparation, the metabolites can poison plant parasitic nematodes, in particular Heterodera glycines, Meloidogyne spp. and Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp. and Aphelenchoides besseyi with good application and development prospects.

The invention discloses a novel chitinase gene of nematode bio-control fungi lilac paecilomyces with a gene sequence table as seen in SEQ ID NO: 1, and meanwhile discloses a method for cloning the chitinase gene of the lilac paecilomyces. The method comprises the steps of using lilac paecilomyces RNA with highest enzyme activity as a template, designing and expanding a pair of degenerate primers to get the chitinase gene fragments of the lilac paecilomyces, obtaining gene 5' terminal and 3' terminal sequences from a lilac paecilomyces cDNA chain through SMART RACE RT-PCR technology, and connecting the three sequences to obtain a novel cDNA full-length sequence of the chitinase gene (PL Chil). The invention provides important technological base for transforming the chitinase gene of the lilac paecilomyces to other nematode bio-control fungi by using bioengineering technology, realizing the heterologous expression of the gene, and improving the expression quantity of the chitinase gene of the nematode bio-control fungi and the bio-control ability of plant nematodes.

The invention discloses a bacillus with nematocidal activity, and a preparation method and an application of the bacillus. The name of a strain of the bacillus is B-RS-06, and the preservation number is CGMCCNo.5352 (China General Microbiological Culture Collection Center Number 5352). The strain is separated from rhizosphere soil of a plant, and metabolin of the strain acts on a plant nematode. A raw material of a nematocide of the bacillus is derived from the rhizosphere soil of the plant, and has no adverse effect on an environment and all organisms such as the plant, people and an animal; the production cost is low; a production process is simple; and the environment is not polluted.

The invention relates to a nematicidal composition containing fosthiazate. The composition comprises an effective component A and an effective component B, wherein the effective component A is fosthiazate, the effective component B is chitosan or matrine, and the weight ratio of the component A to the component B is (50-1):(1-50). The composition can be applied to pesticide manufacturing and prepared into various preparations allowed in agriculture. The composition is reasonable in component compounding and good in plant nematode prevention effect, the nematicidal effect of the composition is not simple combination of activity of the components, and compared with a single preparation, the composition is obvious in synergistic effect and more excellent in nematicidal effect. In actual application, pesticide residues are reduced, the pesticide consumption is low, the service life of pesticides is prolonged, and drug resistance of pests is delayed. Chitosan and matrine serve as biological pesticide and is environmentally friendly and safe.

The invention discloses a rapid PCR (Polymerase Chain Reaction) molecular detection method for ditylenchus destructor thorne and the application of the rapid PCR molecular detection method, and belongs to the technical field of molecular detection of plant nematodes. A PCR system is involved in the rapid PCR molecular detection method for the ditylenchus destructor thorne. The method is characterized in that the PCR system comprises a group of specific primers, wherein the specific primers are DdF1 and DdR1, and can specifically amplify fragments with lengths of 495bp from A-type and B-type ditylenchus destructor thorne, thereby realizing the rapid molecular detection of the ditylenchus destructor thorne. The method is high in sensitivity and specificity, rapid and accurate, and has high actual application value in the aspects of early diagnosis of ditylenchus destructor thorne, field monitoring and early warning and the like.

The invention discloses a method for breeding, culturing and storing plant nematodes by using sweet potato calluses. The method is implemented through the following steps of: cleaning fresh sweet potatoes, performing surface sterilization, peeling, slicing, placing into a culture dish, and culturing in an incubator to obtain sweet potato calluses; inoculating sterilized plant nematodes onto the sweet potato calluses; placing into respective incubators of appropriate temperatures, and culturing and breeding in dark; and after culturing for a certain period of time, storing the plant nematodes and the sweet potato calluses at the temperature of 4-20 DEG C. Due to the adoption of the method, the aims of manually culturing and breeding a large quantity of plant nematodes in a simple way and storing plant nematode resources for a long time can be fulfilled.

The present invention belongs to the field of biological pesticide technology, and is especially one fungus strain with nematocidal effect and its culturing method, metabolic product and application. The fungus strain is Penicillium pinophilum snf44-1 in the preservation number of CGMCC No. 1852. It is prepared through liquid fermentation culturing, and its metabolite has obvious poisoning effect on plant nematode, especially on Meloidogyne hapla. The present invention has excellent application and development foreground.

The present invention provides roundworm egg detecting real-time fluorescence PCR primer and probe and the detection method therewith. Roundworm egg causing contamination on kraut and other botanical product is hard to detected morphologically with microscope. Through designing superfamily roundworm primer and probe to separate out suspected parasitic egg from kraut, repeated freezing and thawing with liquid nitrogen and fluorescent PCR operation, the suspected parasitic egg from kraut may be identified in high accuracy. The present invention has sensitivity enough high for detecting single roundworm egg, high specificity, and no cross reaction with other parasite egg and plant nematode egg.

The invention provides a carbon disulfide fumigation compound agent for killing insects, bacteria and nematode, which is formed by combining carbon disulfide fumigation insecticide and a fumigation medicament with the function of killing insects, bacteria and nematode. The compound agent is characterized in that the compound agent consists of the carbon disulfide fumigation insecticide, chloropicrin, o-dichlorobenzene, sulfuryl fluoride, sulfuryl chloride, chlorine gas, carbon dioxide or nitrogen as inert gas; two components are combined into the carbon disulfide fumigation compound agent for killing insects, bacteria and nematode according to weight portion; three components are combined into the carbon disulfide fumigation compound agent for killing insects, bacteria and nematode according to weight portion; four components are combined into the carbon disulfide fumigation compound agent for killing insects, bacteria and nematode according to weight portion; and five components are combined into the carbon disulfide fumigation compound agent for killing insects, bacteria and nematode according to weight portion. The combination implementing proposal of the invention performs compounding according to the fumigation and pest removal of plant quarantine, health quarantine, logs, containers, soil, commodity maintenance, scrapped vessels, animal-plane specimen museums and the like, and has great social benefit and economic benefit.

The invention provides a new combination of two pairs of universal amplification primers for plant parasitic nematode ribosome ITS (internal transcribed spacer) and an application method thereof for PCR (polymerase chain reaction) amplification. The key innovation point of the method is that after the new primer combination is applied to the PCR amplification of the plant parasitic nematode ITS, the plant nematode type which can be amplified by the new PCR amplification system has broader spectrum, the amplification efficiency and success rate are remarkably improved, and the problems of the ITS amplification in the plant nematode identification before are effectively solved. The PCR amplification product amplified by the method provided by the invention meets the needs of the molecular detection technologies such as nematode DNA (deoxyribonucleic acid) barcode identification and RFLP (restricted fragment length polymorphism) enzyme digestion identification, and can be popularized to the quarantine identification of the port and agriculture / forestry departments in China.

The invention discloses a bacillus cereus BCCY22, the preservation number of which is CGMCC No. 16672, and further discloses a culture method of the strain, a formed microbial inoculum, a preparationmethod of the microbial inoculum and an application of the microbial inoculum. The bacillus cereus colony is milky white, approximately circular and slightly long, has uneven edges, uniform and opaquetexture, and the bacillus cereus BCCY22 is rod-shaped, has the length of 2.86-4.44um and the width of 0.80um-1.21um, and is in chain arrangement or exists independently; the bacillus cereus BCCY22 produce spore, wherein the spore is generated in the middle and is ellipse; cysts do not expand, and Gram staining reaction is positive; the bacillus cereus can utilize glucose and sucrose, and cannot utilize lactose. The bacillus cereus is a broad-spectrum nematode antagonistic strain, and the prepared microbial inoculum has high-efficiency prevention and treatment effects on plant nematode diseases, has certain effects of promoting crop growth and increasing yield, and is favorable for protecting environment.

The invention discloses a stewartia sinensis herba lycopi vinegar liquid and a preparation method thereof, belonging to the technical field of development and research of plant source pesticide. The preparation method of the stewartia sinensis herba lycopi vinegar liquid comprises the following steps: cutting stewartia sinensis herba lycopi into short sections, adding the sections into a dry distillation kettle, heating the dry distillation kettle, condensing, collecting all distillates at the temperature of 90-300 DEG C and collecting liquid of the stewartia sinensis herba lycopi vinegar liquid of the invention at 12h+ / -20min. The stewartia sinensis herba lycopi vinegar liquid has an effective nematode-killing activity against plant nematodes, and the plant nematode killing effect of liquid with 25-150 times of the vinegar liquid can reach 70-100%. The preparation method has simple technique; and compared with methods of extracting by organic solvents, the preparation method has the advantages of lower cost and environment protection.

The invention provides a heterodera filipjevi Taqman MGB probe real-time quantitative PCR detection method and application thereof, belongs to the technical field of plant nematode molecular detection, and discloses a specific fluorescence probe HF-probe and a group of specific primers qHF-R and qHF-F, which are designed on the basis of a heterodera filipjevi RAPD specific sequence. The probe and the primers can be amplified in heterodera filipjevi to obtain specific fluorescence curves, so that the quick molecular detection of heterodera filipjevi is achieved. The detection method has high sensitivity and strong specificity, is fast and accurate, and has high practical applied values in the aspects of early diagnosis and field monitoring and early warning of heterodera filipjevi.

The invention discloses bacillus cereus for preventing and controlling plant nematodes and application thereof. The name of the bacillus cereus is MH61-63-03; the collection number is CGMCC No.15616.Experimental results prove that the bacillus cereus has the advantages that a fermenting filtrate of the bacillus cereus MH61-63-03 has higher toxic activity on second instar larvae of the plant nematodes; the number of nematodes in wheat roots treated by the fermenting filtrate MH61-63-03 is greatly reduced 7 days after the second instar larvae of the cereal cyst nematode are inoculated, and thereduction rate of insect is 26.82%; the number of sporocysts of each wheat plant treated by the fermenting filtrate MH61-63-03 is obviously reduced 65 days after the second instar larvae of the cerealcyst nematode are inoculated; the biological agent has good application and development prospect in the preventing and controlling of the plant nematodes.

The invention relates to a cereal cyst nematode Ha34609 protein, an encoding gene and application of the encoding gene. The amino acid sequence of the cereal cyst nematode Ha34609 protein is shown as SEQ ID NO: 1. The nucleotide sequence of the encoding gene is shown as SEQ ID NO: 2. After a silent Ha34609 gene is treated by adopting dsRNA (double-stranded ribonucleic acid), compared with eGFP (enhanced green fluorescent protein) dsRNA, the length and the width of a white female worm are remarkably reduced (t-test, confidence interval: 95%), which indicates that the gene plays an important role in parasitic and pathogenic processes of cereal cyst nematodes and can serve as a target gene of plant nematode resistance engineering. The cereal cyst nematode Ha34609 protein, the encoding gene and the application of the encoding gene have significant values in a study on a nosogenesis of the cyst nematodes and preparation of nematode resistance plants.

The invention relates to a thiazole phosphine and metalaxyl granule and a preparation method thereof. The thiazole phosphine and metalaxyl granule includes the components by weight: 0.1%-20% of thiazole phosphine, 0.1%-5% of metalaxyl, 70%-80% of carrier attapulgite particles, 0.5%-3% of a dispersing agent, 0.1%-2% of a sustained-release agent, 0.1%-5% of a binder, and the balance a filler. The granule of the present invention is granulated by a film coating method. The invention effectively combines the control of plant nematodes and soil-borne diseases, uses resources efficiently, scientifically and rationally, has important significance on environment protection, and plays a positive role in reducing agricultural inputs and promoting agricultural output and income.

The invention relates to a composition used for controlling plant diseases. The effective components of the composition are iprodione (A) and an active component hymexazol (B), wherein a mass ratio of A to B is 100:1-1:100. When A and B are combined, a synergistic effect is provided for fusarium wilt controlling. Also, the composition can be used for controlling plant threadworms. The composition can be used in disease controlling of plant living bodies such as fruit trees, vegetables, alimentary crops, oil crops, sugar crops, cotton and bast fiber crops, and gardening plants, or growth sites thereof. Especially, the composition is used for controlling nematodiasis, fusarium wilt, and root rot.

The invention relates to a bacteria strain for preventing and controlling plant nematodes and application thereof. The provided bacteria strain Y149 belongs to Massilia sp., and has been collected into China Center for Type Culture Collection on November 27th, 2017; the address of the collection unit is Wuhan University in Wuhan, China, and the collection number is CCTCC NO:M 2017745. The invention also provides a microorganism agent containing the bacterial strain Y149 and a preparation method thereof; the bacterial strain Y149 or the microorganism agent is applied to prepare the product forpreventing and controlling the plant nematodes. The bacterial strain Y149 or the microorganism agent has good preventing and controlling effect on the plant nematodes; the cost is low, and the environment-friendly effect is realized.

The invention discloses a pretreatment method for plant nematodes for detecting MALDI-TOF MS. The pretreatment method comprises the following steps: (1) dropwise adding an extracting liquid on sterilized slide glass, wherein the dropwise addition amount of the extracting liquid is 5-8mu l per plant nematode; (2) transferring the cleaned plant nematodes into the extracting liquid through a stereoscopic microscope, and crushing the plant nematodes, wherein the extracting liquid is used for extracting the protein of the plant nematodes and hydryolyzing the protein into polypeptide; (3) with the stereoscopic microscope, pipetting the extracting liquid in the step (2) and the crushed plant nematode through a pipette, dropwise adding the extracting liquid onto a sample plate, and air-drying; and (4) adding a matrix solution on the air-dried sample plate so as to enable the polypeptide to be dispersed on the matrix solution, and air-drying to obtain the sample for detecting MALDI-TOF MS, wherein the addition amount of the matrix solution is 1-1.5mu l per plant nematode. The method is easy to operate, good in result repeatability, low in background signal and high in sensitivity.

The invention relates to a culturing method for a fungus having plant nematode poisoning activity and a preparation method of a metabolite of the fungus, belonging to the technical field of microbial pesticides. A fungus strain Snef5 related to the invention is gloeotinia nematicida which is collected in the China General Microbiological Culture Collection Center (Address: Datun Road, Chaoyang District, Beijing, Post Code: 100101) with the collection number CGMCC No.4549 on January 24, 2011. The taxonomic statuses of the fungus strain Snef5 are ascomycota, ascomycetes, helotiales and sclerotiniaceae, and gloeotinia belongs to a fungus. The fungus is prepared by performing liquid fermentation culturing. A metabolite of the fungus has very high toxicity on major pathogen nematodes in plant nematodes such as root-knot nematodes, soybean cyst nematodes and aphelenchoides besseyi, a remarkable nematode killing effect and good application and development prospects.

The invention provides a sampling device used for plant nematode and soil nematode and a detection method thereof, relating to the plant nematode and the soil nematode. The sampling device is provided with a box body, an atomizer and at least one set of separation device; the box body is provided with a movable box cover; the position in the box body close to the bottom is provided with a grid-typed cushion plate; one side of the box body close to the bottom is provided with an drainage valve; the atomizer is arranged at the center of the box body; the separation device is provided with a screen, a screen bracket and nematode receiving utensils; the screen is sheathed on the screen bracket and arranged above the nematode receiving utensils; and the separation device is put on the grid-typed cushion plate. Plant root samples or soil samples are put in the screen; the box cover is opened, the screen bracket is sheathed on the nematode receiving utensil, subsequently, the screen with embedded samples is arranged on the screen bracket; the separation device in the sampling device of plant nematode and the soil nematode is uniformly put in the box body and the box cover is covered; the power of the atomizer is turned on and the atomizer generates water mist; after 12-24 hours, the power is turned off, the box cover is opened and the nematode receiving utensils are sequentially taken out and put under a binocular dissecting microscope and an advanced microscope so as to check the nematode and the types of the nematode.