31 results about "Plant nematode" patented technology

Provided is a stabilized aqueous hydrogen peroxide composition comprising a sugar alcohol that does not contain silver, organophosphonate, peroxyacetic acid or other peracid, or stannate. The composition optionally includes a carboxylic acid. The composition is suitable as a disinfectant and oxidizer, and for the treatment, inhibition, or eradication of fungi or nematodes. Also provided is a method for reversing a bacterial or fungal infection in an infected plant, and a method for enhancing plant growth and / or productivity. Also provided is a method for inhibiting or preventing fungal spore germination in soil in a fungal infested area. Also provide is a method of controlling or preventing nematode infestation of a plant, and a method of reversing damage of a plant caused by nematode infestation. Also provided is a method for treating or reversing Panama disease in an infected banana plant.

The invention relates to the technical field of organic chemistry, in particular to application of pyrazine compounds in the aspect of killing nematodes. The pyrazine compound has good nematicidal activity on pine wood nematodes, root-knot nematodes and aphelenchoides besseyi, and part of the pyrazine compound also has good activity on root knot nematode diseases caused by the root-knot nematodes, so that the pyrazine compound has good application prospects in the aspect of root knot nematode disease resistance.

The application discloses application of 3-(4-fluorophenyl) prop-2-yne carbonitrile in prevention and treatment of plant root knot nematodes and belongs to the technical field of plant root knot nematode prevention and treatment. 50 It is found for the first time that 3-(4-fluorophenyl) prop-2-yne carbonitrile has significant insecticidal activity on southern root knot nematodes, the LC 3.109 μg / mL of 3-(4-fluorophenyl) prop-2-yne carbonitrile on southern root knot nematodes is 72 h, the average mortality reaches 92.5% when the application concentration is 48 μg / mL, and the 3-(4-fluorophenyl) prop-2-yne carbonitrile can be used as a novel and effective drug for plant root knot nematode prevention and treatment.

The application discloses application of a benzamide compound in prevention and treatment of plant root-knot nematodes. N‑ (2-ethyl-1,2,5-thiadiazol-4-yl)-2-iodobenzamide. 2H The application discloses a benzamide compound N‑ (2-ethyl-1,2,5-thiadiazol-4-yl)-2-iodobenzamide. 2H The benzamide compound has strong toxic killing effect on various plant root-knot nematodes, and has no insecticidal activity on non-plant pathogenic nematodes, and has the characteristics of high efficiency, low toxicity and low residue. The application provides a new candidate compound for the prevention and treatment of plant root-knot nematodes, enriches the structure type of the root-knot nematode killing benzamide compound, and provides a new thought and way for development of a new root-knot nematode killing pesticide.

The present invention relates to a root rot nematode infection-related pathogenicity gene Pc‑CZ , its encoded protein and its application, aiming to solve the technical problem that root rot nematodes are difficult to control. Pc‑CZ The nucleotide sequence of the gene is shown in SEQ ID NO: 1, and the amino acid sequence of the protein encoded by it is shown in SEQ ID NO: 2. Pc‑CZ The expression of the gene (such as silencing or down-regulating its expression) can inhibit the infection and / or pathogenicity of the root rot nematode to the plant; Pc‑CZ The gene plays an important role in the reproduction, infection and pathogenicity of coffee nematodes and can be used as a target gene for plant nematode resistance engineering. The invention has great application value in the study of the pathogenic mechanism of root rot nematodes and the breeding of nematode-resistant plants.

The invention discloses application of 3-(4-fluorophenyl) propan-2-acetylene nitrile to prevention and treatment of plant root-knot nematodes, and belongs to the technical field of prevention and treatment of plant root-knot nematodes. It is found for the first time that 3-(4-fluorophenyl) propan-2-alkyne nitrile has remarkable insecticidal activity on meloidogyne incognita, LC50 of 3-(4-fluorophenyl) propan-2-alkyne nitrile on meloidogyne incognita is 3.109 micrograms / mL in 72 h, the average death rate reaches 92.5% when the application concentration is 48 micrograms / mL, and 3-(4-fluorophenyl) propan-2-alkyne nitrile can serve as a brand new and effective drug choice for preventing and treating plant meloidogyne.

The present invention generally relates to a composition comprising Bacillus subtilis having a nematicidal effect against plants and / or plant nematodes on their habitats, its use, a process for its preparation, the use of Bacillus subtilis, the prevention, control, extermination and / or imparting of resistance against plant nematodes, and a kit. In particular, the present invention relates to Bacillus subtilis (DSM 32324).

The invention relates to the technical field of animal and plant parasite prevention and control, in particular to application of cryptolepine and derivatives thereof to prevention and control of nematodes, including prevention and control of plant nematodes and / or animal nematodes. Insecticidal tests prove that cryptolepine and derivatives thereof have remarkable killing activity on plant nematodes and animal nematodes, and are expected to be widely applied in the aspects of agriculture and animal husbandry.

The present invention belongs to the field of microorganisms, and specifically relates to a Trichoderma strain antagonistic to melon root-knot nematode disease and wilt disease and its application. Trichoderma breve Application of HLST422‑7 in the control of root-knot nematodes and wilt diseases. Trichoderma breve There are no reports on the use of HLST422‑7 in the prevention and treatment of plant nematode diseases, and there has never been any relevant research on the combined infection of nematodes and wilt. T.short Compared with T069, Trichoderma breve HLST422‑7 has better control effects on root-knot nematodes and wilt, and can also improve plant growth in the presence of nematodes, further demonstrating the great advantages of this strain in plant disease prevention and control.

The application discloses application of bacillus velezensis LH-BLS107 (CGMCC No. 34785) or an engineering bacterium modified through natural, artificial mutagenesis or gene editing in biological control of plant nematode diseases. Fermentation culture solution, bacterium powder and / or secondary metabolites of the LH-BLS107 or the engineering bacterium thereof are used in wheat, soybean, rice, fruit trees, vegetables and the like. The fermentation culture solution, the bacterium powder and / or the secondary metabolites can effectively prevent and control wheat cyst nematode disease, soybean cyst nematode disease, rice paratylenchus pseudohollandei disease, fruit tree and vegetable root knot nematode disease, and the mortality of the above-mentioned nematodes can reach more than 95%, and when the fermentation culture solution, the bacterium powder and / or the secondary metabolites are compounded with chemical nematocide, the chemical agent dosage can be reduced by 25%-30%.

A method of treating and / or preventing a disease in plants, including a step of administering a composition comprising phenoxyethanol to a plant is disclosed. In addition, a method of treating and / or preventing a nematodal infestation in plants, comprising a step of administering a composition comprising phenoxyethanol to a plant is disclosed. Compositions comprising phenoxyethanol used in such methods are also disclosed.

The invention belongs to the technical field of pesticides, and discloses a fluopyram-containing pesticide composition and application thereof.The pesticide composition comprises an active component A and an active component B. The active component A is fluopyram, the active component B is d-limonene, and the mass ratio of the active component A to the active component B is 1: 30-35: 1. The composition disclosed by the invention has an excellent prevention and treatment effect on plant nematodes, has a remarkable synergistic interaction effect under a certain mass ratio, can effectively reduce the use amount of pesticides and reduce pesticide residues of crops, and is environment-friendly.

The plant nematode generation and culture device comprises a shell, a steam absorber, a humidifier and a control box, a cover plate is arranged at an opening of the shell, an experiment generation chamber, a material placing chamber and a bottom layer cavity are arranged in the shell, and a humidity sensor is arranged in the material placing chamber; an air suction port of the steam absorber is communicated with the experiment generation chamber; an air outlet pipe of the humidifier is communicated with the material placing chamber; a control unit, a data acquisition unit and a wireless communication unit are arranged in the control box, the data acquisition unit is electrically connected with the humidity sensor, the wireless communication unit is in wireless communication connection with the mobile terminal, and a display unit is arranged on the outer side of the control box. During use, the steam absorber absorbs steam in the experiment generation chamber, the humidifier adjusts the humidity in the material placement chamber, the control box can display the humidity and the like in real time, and particularly, parameters such as the humidity and the like can be transmitted to a mobile terminal through the wireless communication unit, so that real-time monitoring is realized, and the plant nematode generation and culture requirements are met.

The application belongs to the field of plant protection, and specifically provides application of a thiazole compound in preparation of a medicine for preventing and treating plant nematode diseases. The thiazole compound includes 5-(chloromethyl)-3-(2,4-dichlorothiazol-5-yl)-1,2,4-oxadiazole, 5-(bromomethyl)-3-(2,4-dichlorothiazol-5-yl)-1,2,4-oxadiazole and the like. Through nematode-killing activity tests on Bursaphelenchus xylophilus, Meloidogyne incognita and Aphelenchoides besseyi, it is found that the compounds have good and broad-spectrum nematode-killing activity.

The invention provides an Enterobacter sp (Enterobacter sp) strain and an application thereof, and relates to the technical field of biocontrol microorganisms. The strain is preserved in the China Center for Type Culture Collection on November 1, 2019, and the preservation number is CCTCC NO: M 2019830. The strain has a good control effect on plant nematodes and has a remarkable growth promoting effect on plants. In addition, the method is low in cost, environment-friendly and wide in application prospect in the field of rice planting.

The application provides application of linear dipeptide Ala-Leu in prevention and treatment of nematodes. The linear dipeptide Ala-Leu from plants has the activity of killing various nematodes, including common root knot nematodes, pine nematodes and other plant nematodes and free-living nematodes such as Caenorhabditis elegans, and can obviously kill insects at a low dose; potting experiments prove that the linear dipeptide can prevent and treat plant diseases caused by nematodes. Moreover, the linear dipeptide Ala-Leu has the advantages of small molecular weight, simple structure, low cost, good heat resistance and stability, and is easy to be degraded in animal cells, is safe for humans and other animals, has no activity of inhibiting the growth of bacteria and fungi, is safe for non-target organisms, does not affect the soil bacterial community structure, and has high application value in development of an environmentally-friendly, safe and efficient new type of nematocide and a new type of agent for preventing and treating plant nematode diseases.

The application discloses application of 2-chloro-N(4-chlorophenyl)acetamide to prevention and treatment of plant root-knot nematodes and belongs to the technical field of prevention and treatment of plant root-knot nematodes.The chlorophenylamide compound is 2-chloro-N(4-chlorophenyl)acetamide and / or a pharmaceutically acceptable salt thereof.It is found for the first time that the cyclic amide 2-chloro-N(4-chlorophenyl)acetamide has significant killing activity on southern root-knot nematodes, the half lethal concentration LC 50 of 24h is 2.31 μg / mL, and the half lethal concentration LC 50 of 48h is 2.17 μg / mL, has the characteristics of good specificity, high efficiency, low toxicity, is environment-friendly, and meets the needs of green plant protection and sustainable agricultural production and development.

The invention provides a quick-acting theasaponin composite plant nematode control agent, a preparation method and application, and the quick-acting theasaponin composite plant nematode control agent is prepared from the following raw materials in parts by weight: 10 parts of theasaponin, 1-2 parts of modified theasaponin, 0.5-1 part of isoprophos and 0.01-0.03 part of a cosolvent, the modified theasaponin is prepared by a method comprising the following steps: 1) carrying out esterification reaction on alcohol and an arteannuic acid derivative under the catalytic action of a catalyst to obtain an arteannuic acid ester derivative; and 2) carrying out transesterification on the arteannuic acid ester derivative and theasaponin under the catalytic action of a catalyst to obtain the modified theasaponin. The prevention and treatment agent is prepared by modifying theasaponin by using an arteannuic acid ester derivative with a terpenoid structure, the modified theasaponin has a good binding capacity with a plant nematode cell membrane, can enhance the permeability of the cell membrane and destroy the structure and function of the cell membrane, and can enable nematodes to die quickly in a short time by cooperating with a nerve toxic agent drug iprophos.

The invention discloses a novel insecticidal protein as well as a coding gene and application thereof, and belongs to the technical field of biological control. The invention provides a novel insecticidal protein Rknp1. The amino acid sequence of the novel insecticidal protein Rknp1 is as shown in SEQ ID NO. 1. A biological activity experiment result shows that the insecticidal protein Rknp1 provided by the invention can be used for efficiently preventing and treating pests such as nematodes, culex and the like, the killing effect of the object ear bean root-knot nematode is particularly remarkable, and LC50 is 109.87 mu g / mL; the killing rate of 300 [mu] g / mL insecticidal protein on nematodes and culex is 90% or above. The insecticidal protein Rknp1 provided by the invention can be used for prevention and control of pests, provides a new gene resource for biological prevention and control of pests such as plant nematodes and cultivation of pest-resistant transgenic plants, and has a wide application prospect.

The invention belongs to the technical field of microorganisms, and particularly relates to paecilomyces lilacinus as well as a fungicide and application thereof. The invention discloses a novel paecilomyces lilacinum Pli-25, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.41932, and the 16S rDNA (Reverse Deoxyribose Nucleic Acid) sequence of the paecilomyces lilacinum Pli-25 is as shown in SEQ ID NO: 1. A liquid-state fermentation process and a solid-state fermentation process of the strain are studied, a liquid-state fungicide and a powdery fungicide of the paecilomyces lilacinus strain Purpureocillium lilacinus Pli-25 are developed and obtained, a field test result shows that the prevention and treatment effect on the plant field nematode disease by applying the fungicide is remarkable, a new microbial resource and fungicide are provided for biological prevention and treatment of the plant nematode disease, and the application of the fungicide to the field nematode disease is facilitated. The application prospect is wide.

The invention discloses application of 2-chloro-N (4-chlorphenyl) acetamide to prevention and treatment of plant root-knot nematodes, and belongs to the technical field of prevention and treatment of plant root-knot nematodes, and chlorobenzamide compounds are 2-chloro-N (4-chlorphenyl) acetamide and / or pharmaceutically acceptable salts of the 2-chloro-N (4-chlorphenyl) acetamide. It is found for the first time that cyclic amide compound 2-chloro-N (4-chlorphenyl) acetamide has remarkable killing activity on meloidogyne incognita, the median lethal concentration LC50 in 24 h is 2.31 mu g / mL, the median lethal concentration LC50 in 48 h is 2.17 mu g / mL, and the cyclic amide compound 2-chloro-N (4-chlorphenyl) acetamide has the advantages of being good in specificity, efficient and low in toxicity, is environmentally friendly and meets the requirements for green plant protection and sustainable agricultural production and development.

The present invention generally relates to compositions comprising Bacillus subtilis with nematicidal effect against phytonematodes on plants and / or its habitat, to its use, to a process for its preparation, to the use of Bacillus subtilis, to preventing, controlling, combating and / or conferring induction of resistance to phytonematodes and to a kit. Particularly, the invention relates to Bacillus subtilis (DSM 32324).

The present invention provides a composition for coating agricultural seeds with agriculturally important microorganisms. A composition according to the present invention provides stabilization and viability of agriculturally important microorganisms in the coating of stored seeds, resulting in long shelf life. The present invention further provides a method for preparing a composition for coating seeds with viable microorganisms, as well as a method for coating seeds with a composition according to the present invention. The agriculturally important microorganisms according to the present invention possess multiple biological functions, including but not limited to plant growth promotion, nutrient mobilization, and control of phytonematodes and phytopathogens.

The invention relates to application of 1-octylene-3-ketone in prevention and treatment of plant nematodes, and belongs to the field of plant disease and insect pest prevention and treatment. 1-octylene-3-ketone with different concentrations is used for contact-killing or fumigating various nematodes such as northern root-knot nematodes, elephant bean root-knot nematodes, grape root-knot nematodes, bursaphelenchus xylophilus, bursaphelenchus xylophilus and panoda resurrection nematodes; the bactericidal composition has strong toxic activity, can be used for effective prevention and control of plant nematode diseases, and provides a new medicament for prevention and control of plant nematode diseases.

The invention discloses 24 plant nematode pedigree specific genes in ditylenchus destructor and application of dsRNA of the 24 plant nematode pedigree specific genes in nematode prevention and control, and belongs to the technical field of gene engineering. CDS nucleotide sequences of the 24 specific genes of the plant nematode lineage are as shown in SEQ ID NO: 1-24, and nucleotide sequences of positive-sense strands of dsRNA of the 24 specific genes of the plant nematode lineage are as shown in SEQ ID NO: 25-48. The parasitic ability of the nematodes to hosts after dsRNA treatment is determined through an in-vitro interference method, the expression levels of the 24 plant nematode pedigree specific genes are all remarkably reduced after the 24 plant nematode pedigree specific genes are treated by respective corresponding dsRNA fragments, and the planting ability and the infection area of the ditylenchus destructor are reduced by 20-59% and 22-72% respectively after the 24 plant nematode pedigree specific genes are inoculated into sweet potatoes for 25 days; results show that the specific genes of the 24 plant nematode pedigree participate in regulation and control of infection and reproduction of nematodes on hosts, and can be used as target genes for development of plant nematode prevention and control drugs.

The application belongs to the technical field of biology and specifically relates to a clavate branch of Clonostachys rosea and application thereof in preparation of a medicine for preventing and treating a plant pathogenic nematode, wherein the clavate branch of Clonostachys rosea has a preservation number of CCTCC NO: M20211658. The clavate branch of Clonostachys rosea provided by the application can prevent and treat plant nematode diseases efficiently and promote the growth of plant root height. Meanwhile, the fermentation technology of the clavate branch of Clonostachys rosea is optimized, and a fermentation medium with the highest activity of metabolic products is screened. In addition, the fermentation technology is simple, and the production equipment investment and production cost are greatly reduced. The preparation for preventing and treating nematode diseases provided by the application is a biological preparation, which is safe to use and does not cause drug damage and secondary pollution.

The utility model provides a pathogenicity test device for plant nematodes, which relates to the technical field of plant nematodes and comprises a support, a constant-temperature box and a placing plate, and a sealing door is arranged on the side face of the constant-temperature box. A bevel gear A rotates by rotating a rotating handle, the bevel gear A and a bevel gear B are in meshing transmission, so that a bidirectional lead screw is driven to rotate, a sliding block A can drive limiting plates to move in the same direction or in the opposite directions, limiting of a containing plate is relieved, and then the containing plate can be detached; when placing plates of different specifications need to be replaced or cleaned and disinfected, operation can be rapidly and conveniently carried out, a water falling basin, a filter plate, a water inlet pipe, a water outlet pipe, a water sprinkling plate and a water pump are designed to form a water circulation system, then the soil humidity in the planting basin can be kept relatively stable, water flowing is beneficial to distribution of nematodes in soil, and the nematodes are prevented from being damaged. The natural infection state is better met, and the pathogenicity of the nematodes to plants can be researched easily.

The present invention discloses the use of N-(1-acetyl-2,3-dihydro-1H-indol-6-yl)-2,6-difluorobenzamide in preventing and controlling plant root-knot nematodes. The present invention is the first to discover that N-(1-acetyl-2,3-dihydro-1H-indol-6-yl)-2,6-difluorobenzamide has a strong toxic effect on a variety of plant root-knot nematodes, with a median lethal dose (LC50) of 100% for southern root-knot nematodes, javanic root-knot nematodes, and elephant ear bean root-knot nematodes within 24 hours. 50 The concentrations were 4.27 μg / ml, 8.38 μg / ml and 7.51 μg / ml respectively; the median lethal dose LC of the southern root-knot nematode, the javanic root-knot nematode and the elephant-ear root-knot nematode was 48 hours. 50 The concentrations are 4.11 μg / ml, 9.56 g / ml and 6.92 μg / ml respectively. It has no toxic activity against non-plant pathogenic nematodes such as Caenorhabditis elegans, has the characteristics of high efficiency and low toxicity, and can be used in the development and research of root-knot nematode insecticides.

The application discloses a biological microcapsule preparation and a preparation and application thereof, and aims to solve the technical problems of a short shelf life, a great influence of external environmental factors, poor stability and the like of an existing biocontrol preparation. The biological microcapsule preparation is prepared by embedding a pink Clonostachys rosea strain CGMCC No. 16262, spores or / and metabolites thereof with a embedding agent sodium alginate, a filler and an auxiliary agent algal oligosaccharide. The filler is at least one of soluble starch and bentonite. The biological microcapsule preparation has a long shelf life, and can significantly improve the acid and alkali resistance, salt resistance, ultraviolet resistance and high-temperature resistance of the pink Clonostachys rosea strain, and the effects of the biological microcapsule preparation in plant nematode disease prevention and yield increase promotion are significantly higher than those of other dosage forms. In addition, the microcapsule single-dose treatment or the microcapsule reduced-dose compound treatment with trifopyrifenam can significantly improve the control effect on root-knot nematode disease, achieves the purpose of pesticide reduction and efficiency increase, and has a good application prospect.

This invention relates to the application of 1-octen-3-one in the control of plant nematodes, belonging to the field of plant pest and disease control. This invention uses different concentrations of 1-octen-3-one to kill or fumigate various nematodes, including Northern Root-Knot Nematode, Elephant Root-Knot Nematode, Grape Root-Knot Nematode, Luoding Sporangia Nematode, Pine Wood Nematode, and Whole Tooth Resurrection Nematode, all of which have strong toxic activity and can be used for the effective control of plant nematode diseases, providing a new agent for the control of plant nematode diseases.