Field separation method for tomicus piniperda-associated bacteria

Abstract

The invention discloses a field separation method for tomicus piniperda-associated bacteria. The method comprises the following steps: determining a tomicus piniperda invasion hole on a suffered pine tree in a forest, peeling off the surface bark of the mother gallery of tomicus piniperda pests along the direction of the mother gallery of the tomicus piniperda pests by taking the invasion hole as an origin, so as to expose the phloem tissues of the eaten mother gallery of the tomicus piniperda, if larvae galleries are already formed at the both sides of the mother gallery, exposing the larvae galleries, inspecting and confirming whether the spores, hyphae, perithecia and blue-stain phloem tissues of tomicus piniperda-associated bacteria exist in the various eaten galleries of the tomicus piniperda, and adopting corresponding separation measures for the tomicus piniperda-associated bacteria according to the growth and development progresses and the blue-stain phloem tissue conditions of the tomicus piniperda-associated bacteria. The method disclosed by the invention integrates field sampling with laboratory strain separation, and is simple in steps and convenient to operate; the separation obtaining success rate of the tomicus piniperda-associated bacteria is high; the many links of bringing the wood blocks infected by the associated bacteria or the phloems with galleries, and the blue-stain tissues back to a laboratory at first, and then separating are effectively simplified, the separation time for the associated bacteria is saved, the separation is accurate, and the separation efficiency is increased.

Description

technical field [0001] The invention relates to the technical field of a method for isolating associated bacteria of the bark beetle, and belongs to the field of prevention and control of forest diseases and insect pests. Background technique [0002] The tip-cutting beetle is a borer pest of coniferous trees dominated by pine trees. So far, 7 species have been described, mainly the vertical pit-cutting tip-cutting beetle ( Tomicus piniperda ), Hengkeng beetle ( T. minor ), the yunnanensis beetle ( T. yunnanensis ), the pine bud-cutting beetle ( T. brevipilosus ) and Huashan pine beetle ( T. armandii )Wait. Among them, the Hengkeng beetle and the Huashan pine beetle are currently the main pine tree pests in my country. Especially since the 1980s, these beetle outbreaks have caused disasters in the Yunnan pine forest area, resulting in nearly one million mu of Yunnan pine forest. Totally devastated. Since the beginning of this century, the disaster of the tip-cuttin...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the invention is to solve the problems of large sampling and separation workload, high pollution rate and long separation cycle in the prior art, and propose a cutting method that can effectively reduce the pollution rate and save separation time, and significantly improve the separation efficiency of bark beetle associated bacteria. Field Isolation Method of Accompanied Fungi