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Production method for collaborative increase and stabilization of multiple active substances of cordyceps militaris

A technology of active substances and production methods, which is applied in the field of fruit body production of edible and medicinal fungi, can solve the problems of lack of research on synergistic and stable improvement of various active substances of Cordyceps militaris, and achieve the effect of content increase

Inactive Publication Date: 2020-08-28
SHANXI WANHAIAO BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the development of modern artificial cultivation technology, the research on the active substances of Cordyceps militaris is concentrated on the research of a single active substance, but there is a lack of research on the synergistic and stable improvement of multiple active substances of Cordyceps militaris

Method used

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  • Production method for collaborative increase and stabilization of multiple active substances of cordyceps militaris

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Effect test

Embodiment 1

[0032] A production method for synergistically improving and stabilizing multiple active substances of Cordyceps militaris, comprising the steps of:

[0033] (1) Preparation of an effective compound liquid species of Cordyceps militaris heterothalism:

[0034] Microscopic examination of the mother species: Take the mother species of Cordyceps militaris separated by myself, and observe under the microscope: on the same mycelium, there are imbricate Paecilomyces type and Verticillium type sporulation structure aggregated into heads. Moreover, the quantity of the two types is basically balanced, that is, the effective mother species, and if there is only one of the two types or if it is not balanced, it will be discarded;

[0035] Establish a companion bacterium as the mother species of Streptomyces from the Institute of Pharmacy, Chinese Academy of Medical Sciences, numbered CP20004, anti-tumor Streptomyces;

[0036] Prepare a nutritionally balanced liquid medium: 200g of potatoe...

Embodiment 2

[0054] Embodiment 2 A kind of production method that synergistically improves and stabilizes multiple active substances of Cordyceps militaris, according to the following steps:

[0055] (1) Preparation of an effective compound liquid species of Cordyceps militaris heterothalism:

[0056] Take the patented species, the patent number is ZL201210248399.6, which is the mother species of anti-degeneration Cordyceps militaris. Observe under a microscope: the same mycelium has imbricated Paecilomyces type and Verticillium sporogenous structure aggregated into heads, and the two types are basically balanced, that is, the effective mother species; choose another Streptomyces , that is, Institute of Pharmacy, Chinese Academy of Medical Sciences, No. CP200004; prepare nutritionally balanced liquid medium: 200g potatoes, 30g corn flour, 20g white sugar, 3g peptone, 1.5g potassium dihydrogen phosphate, 0.02g sodium sulfite, 0.01 indole acetic acid g. Water 1000ml; boil the above material...

Embodiment 3

[0067] Embodiment 3 A kind of production method that synergistically improves and stabilizes multiple active substances of Cordyceps militaris, according to the following steps:

[0068] (1) Preparation of an effective compound liquid species of Cordyceps militaris heterothalism:

[0069] The recorded species of Cordyceps militaris was preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation number: CGMCC5577, and the preservation time was December 12, 2011. Microscopic examination was performed on this mother species, and Paecilomyces was found on the same mycelium Two balanced sporulation structures of Verticillium type and Verticillium type are the effective parent species; then take Streptomyces CP200004 as the associated bacteria; prepare a nutritionally balanced liquid medium: 200g of potatoes, 30g of corn flour, 20g of white sugar, peptone 3g, potassium dihydrogen phosphate 1.5g, sodium sulfite 0.02...

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Abstract

The invention discloses a production method for collaborative increase and stabilization of multiple active substances of cordyceps militaris and belongs to the technical field of production of fruiting bodies of edible medicinal fungi. The method comprises: (1) smashing and uniformly mixing rice, wheat and soybean meal, adding a nutrient solution and preparing a growth culture medium; (2) takingcordyceps militaris mother strains which have a conidial fructification structure of both an imbricate paecilomyces type and a capitate-gathering verticillium type on a same hypha as effective motherstrains, taking streptomycete mother strains as associated bacteria, performing inoculation in a liquid culture medium and obtaining effective composite liquid stains; (3) diluting and effective composite liquid strains and inoculating the strains in the growth culture medium for culture of 40-60 days; and (4) collecting fruiting bodies, spraying white liquor soaked with dry pine needles on surfaces of the fruiting bodies and performing drying. The method can promote collaborative increase of contents of the multiple active substances and stabilize production; in particular, contents of anti-cancer substances such as Pentostatin, polysaccharide, cordycepic acid and SOD enzyme can be increased obviously; and the method is applied to industrial generalization and application.

Description

technical field [0001] The invention belongs to the technical field of fruit body production of edible and medicinal fungi, and relates to the cultivation of Cordyceps militaris, in particular to a production method for synergistically improving and stabilizing multiple active substances of Cordyceps militaris. Background technique [0002] Cordyceps militaris is a rare food and therapeutic fungus Chinese herbal medicine, which has a wide range of medical and health value. Studies have shown that Cordyceps militaris contains protein, amino acids, macro elements, trace elements, multivitamins and other essential nutrients, which are higher than other fungal foods. In addition, it is also rich in unique adenosine, cordycepin, spray Active substances such as statin, cordycepic acid, cordyceps polysaccharide, SOD enzyme, and organic selenium. These active substances are combined with Cordyceps militaris to form a large natural formula, which can adjust the physiological functio...

Claims

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Application Information

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IPC IPC(8): A01G18/00
CPCA01G18/00
Inventor 杨毅周文贵杨曼
Owner SHANXI WANHAIAO BIOLOGICAL TECH CO LTD
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