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Rhizosphere growth-promoting water-soluble microbial bacterium manure

A microbial fertilizer and rhizosphere growth-promoting technology, which is applied in the direction of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of difficulty in realizing factory-scale production and industrial application, unstable microbial populations, and easy occurrence Broken and deteriorated problems, to ensure sustainable development, facilitate industrial production, and improve market competitiveness

Active Publication Date: 2015-08-19
山东宝来利来生物工程股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are still many deficiencies in the research on microbial fertilizers in my country, such as the relatively low content of active ingredients in some products, unstable microbial populations, easy degradation of varieties, low reproduction coefficient, and low particle strength. Problems such as breakage and deterioration are prone to occur during transportation
At present, most of the research materials on microbial fertilizers are about the verification of fertilizer efficiency, but as a key step to improve the quality of microbial fertilizers and mass production, there are very few reports on the development process of microbial fertilizers, and they are very general. value, it is difficult to realize factory-scale production and industrial application

Method used

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  • Rhizosphere growth-promoting water-soluble microbial bacterium manure
  • Rhizosphere growth-promoting water-soluble microbial bacterium manure
  • Rhizosphere growth-promoting water-soluble microbial bacterium manure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Screening of functional strains

[0050] 1 test material

[0051] 1.1 Strains

[0052] The 80 strains provided by Shandong Baolai Lilai Bioengineering Co., Ltd. strain preservation room include bacteria, yeast, lactic acid bacteria and filamentous bacteria.

[0053] 1.2 Medium

[0054] Screening medium (Monkina medium) with phosphorus-dissolving functional strains: glucose 1%, (NH 4 ) 2 SO 4 0.05%, MgSO 4 0.03%, NaCl 0.03%, KCl 0.03%, FeSO 4 0.0036%, MnSO 4 0.003%, CaCO 3 0.5%, lecithin 0.02%, agar 1.5%, pH7.2. All are percentages by mass.

[0055] Screening media for strains with photosynthetic function:

[0056] (Medium I): KH 2 PO 4 0.5g / L, K 2 HPO 4 0.6g / L, (NH 4 ) 2 SO 4 1.0g / L, MgSO 4 0.2g / L, NaCl0.2g / L, CaCl 2 0.05g / L, yeast extract 0.1g / L, trace elements 1mL / L, growth factors 1mL / L, pH 6.7;

[0057] (Medium II): KH 2 PO 4 1.0g / L, NH 4 Cl 1.0g / L, MgCl 2 0.2g / L, CaCl 2 0.05g / L, trace elements 1mL / L, growth factors 1mL / L, pH 7; ...

Embodiment 2

[0179] The preparation of embodiment 2 bacterial agents

[0180] 1 test material

[0181] Various nutrient elements for medium preparation;

[0182] 2 test method

[0183] 2.1 Screening of general media

[0184] On the basis of using carbohydrates instead of protein in LB medium to prepare the medium, adding medium and trace elements required for microbial growth to screen the general medium, inoculating the strains obtained from the preliminary screening into the liquid medium, and measuring the OD value and the number of effective viable bacteria as the determination index for screening.

[0185] 2.2 Antagonism test of strains

[0186] Prepare a universal medium, use the growth confrontation method to conduct antagonism experiments on the candidate strains, and observe the antagonism status of the selected strains.

[0187] 2.3 Bacteria preparation

[0188] (1) Formulation design: design the formula according to the dosage form and composition of the current microbial ag...

Embodiment 3

[0205] Example 3 The effect verification of rhizosphere growth-promoting water-soluble microbial fertilizer

[0206] 1 test material

[0207] The rhizosphere growth-promoting type water-soluble microbial bacterial fertilizer prepared in 3.3 in embodiment 2;

[0208] Cabbage variety: commercially available Jingpeng-Qiufu No. 9;

[0209] 2 test method

[0210] 2.1 Test groups: A: Blank Control group (blank group without chemical fertilizers and fertilizers); B: CK-Ⅱ (single application of chemical fertilizer ammonium bicarbonate group, 2000 times water dilution 180kg / hm 2 ); C: CAMP (rhizosphere growth-promoting type water-soluble microbial bacterial fertilizer, is the microbial bacterial fertilizer prepared by the embodiment of the present invention 2, 2000 times of water dilution 180kg / hm 2 ).

[0211] 2.2 Field seedling raising test: Soak cabbage seeds in 30°C water for 1 hour, wrap them in gauze and seal them with oil paper for about 32 hours; plow 1m×4m of land, rake it...

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Abstract

The invention discloses rhizosphere growth-promoting water-soluble microbial bacterium manure. The rhizosphere growth-promoting water-soluble microbial bacterium manure is prepared from culture obtained by carrying out aerobic mixed fermentation on bacillus cereus GF-1, streptococcus thermophilus BLST, bacillus mucilaginosus G3, bacillus subtilis B7348, bacillus subtilis N9-1-35, lactobacillus plantarum LP and candida utilis CUM on a universal culture medium, wherein the total number of biological living bacteria in the microbial bacterium manure is greater than or equal to 2*10<10> CFU / g. The invention also discloses a preparation method of the rhizosphere growth-promoting water-soluble microbial bacterium manure. The microbial bacterium manure disclosed by the invention utilizes exogenous plant growth-promoting bacteria and associated bacteria to improve the micro-environment of root soil, and the prepared microbial bacterium manure is applied into the soil, the soil environment can be effectively improved, the growth of plants is promoted, and the resistances of the plants are improved.

Description

technical field [0001] The invention relates to a rhizosphere growth-promoting water-soluble microbial fertilizer, which belongs to the technical field of microbial compound bacterial fertilizer. Background technique [0002] Animal and plant residues, decaying matter in the soil, and humus formed by compounding with soil colloidal particles with the participation of microorganisms, these substances release various nutrients during the decomposition and weathering process, directly affecting the content and diversity of soil nutrients. The nutrient loss in the soil is mainly through three ways: crop absorption, underground water seepage loss and surface gas volatilization. The release of soil natural nutrients is far from meeting the needs of normal crop development, and external fertilization is required. This not only results in the waste of a large amount of non-renewable resources, but also reduces the fertility of the soil, deteriorates the soil quality, and greatly red...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C12N1/20C12N1/14C12R1/085C12R1/46C12R1/07C12R1/125C12R1/25C12R1/72
Inventor 单宝龙陈雷谷巍侯红燕张建梅王红韩广泉谢全喜黄启亮乔蓬蕾张文于佳民王艳敏侯楠楠孙波王珂王春凤
Owner 山东宝来利来生物工程股份有限公司
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