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Method for improving production capacity of acid producing bacteria by adopting Vc two-step fermentation

A production capacity, two-step fermentation technology, applied in fermentation and other directions, can solve problems such as difficulty in further improving production capacity, unbalanced growth of acid-producing bacteria and associated bacteria, and suppression of the number and activity of acid-producing bacteria. The effect of production efficiency, short fermentation cycle, standardized management and regulation

Inactive Publication Date: 2017-05-10
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the high dependence of acid-producing bacteria on companion bacteria is accompanied by strong negative effects, such as competition between companion bacteria and acid-producing bacteria for nutrients and ecological niches, etc., often suppressing the number and activity of acid-producing bacteria
Therefore, it is not easy to achieve both when cultivating or fermenting.
In practical operation, one tends to lose sight of the other, resulting in an unbalanced growth between acid-producing bacteria and associated bacteria
Therefore, the process has poor process controllability, large batch fluctuations, low overall yield, and difficulty in further improving production capacity.

Method used

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  • Method for improving production capacity of acid producing bacteria by adopting Vc two-step fermentation

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Experimental program
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Effect test

Embodiment 1

[0024] (1) choose 5L fermenter, inject 3L glucose mass content and be the seed culture medium of 0.5%, insert 150ml concentration and be 2 * 10 8 CFU / ml of Bacillus megaterium suspension was used as companion bacteria, and the concentration of companion bacteria reached 3.6×10 8 CFU / ml, and then ultrasonically disrupted at 25KHz to obtain active protein solution.

[0025] (2) Take 100ml of the above-mentioned active protein solution, insert it into a 20L fermentation tank containing 15L of fermentation medium, and then insert 400ml of it into a bacterium concentration of 3×10 9 CFU / ml suspension of acid-producing bacteria (acid-producing bacteria is ketogenic cologne), fermented until the bacterial concentration reaches 4.5×10 9 CFU / ml to obtain the acidogenic bacteria fermentation seed liquid. (3) Get 15L of the above-mentioned acidogenic bacteria fermentation seed solution and insert it into a 500L fermenter containing 300L of fermentation medium and 1.5L of the active pro...

Embodiment 2

[0028] (1) Select a 5L fermenter, inject 3L of seed culture medium with a glucose mass content of 0.5%, and insert 150ml of bacteria with a concentration of 2×10 8 CFU / ml of Bacillus megaterium suspension was used as companion bacteria, and the concentration of companion bacteria reached 3.6×10 8 CFU / ml. 25KHz ultrasonic crushing to obtain active protein liquid.

[0029] (2) Take 100ml of the active protein solution obtained above, insert it into a 20L fermenter containing 15L of fermentation medium, and then insert 400ml of it into a bacterium concentration of 3×10 9 CFU / ml suspension of acid-producing bacteria (acid-producing bacteria is ketogenic cologne), fermented until the bacterial concentration reaches 4.5×10 9 CFU / ml to obtain the acidogenic bacteria fermentation seed liquid.

[0030] (3) Take 15L of the above-mentioned acidogenic bacteria fermentation seed liquid and insert it into a 500L fermenter containing 300L fermentation medium and 1.5L of the active protein...

Embodiment 3

[0033] (1) Choose a 5L fermenter, inject 3L of a seed medium with a glucose mass content of 0.6%, and insert 200ml of a concentration of 3 × 10 8 CFU / ml of Bacillus cereus suspension was used as companion bacteria, and the concentration of companion bacteria reached 5.5×10 8 CFU / ml. 25KHz ultrasonic crushing to obtain active protein liquid.

[0034] (2) Get 100ml of the active protein solution obtained above, insert it into a 20L fermentation tank containing 15L of fermentation medium, and then insert 400ml of it with a concentration of 2.5×10 9 CFU / ml suspension of acid-producing bacteria (acid-producing bacteria is ketogenic cologne), fermented until the bacterial concentration reaches 4×10 9 CFU / ml to obtain the acidogenic bacteria fermentation seed liquid.

[0035] (3) Take 15L of the above-mentioned acidogenic bacteria fermentation seed solution and insert it into a 500L fermenter containing 300L of fermentation medium and 1.5L of the active protein solution obtained i...

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Abstract

The invention relates to the field of industrial biological fermentation, in particular to a method for improving production capacity of acid producing bacteria by adopting Vc two-step fermentation. The method comprises the steps as follows: associated bacteria are cultured independently in a seed culture medium to a stable end stage, and an active protein solution is prepared from an obtained culture solution through cell disruption; the fermentation medium containing the active protein solution is inoculated with a bacterial suspension of the acid producing bacteria, and a fermentation seed liquid is obtained through fermentation; the fermentation medium containing the active protein solution is inoculated with the fermentation seed liquid, the acid producing bacteria perform fermentation, biosynthesis of the sorbose is performed, and gulonic acid is obtained after fermentation. The method is easy to operate, high in stability, short in fermentation cycle, high in recovery rate, good in product quality, low in cost, green and environment-friendly.

Description

technical field [0001] The invention relates to the field of industrial biological fermentation, in particular to a method for improving the production capacity of Vc two-step acid-producing bacteria. Background technique [0002] Vitamin C (Vitamin C, referred to as Vc), also known as L-Ascorbic Acid (L-Ascorbic Acid), is a vitamin that is common in plants and cannot be synthesized by humans. Vc has a variety of functions and is widely used in the fields of medicine, food, feed and cosmetics, and has a huge market demand. The Vc two-step fermentation method is the main method for the industrial preparation of the Vc precursor—gulonic acid at present. Vc two-step fermentation is a mixed fermentation of two kinds of bacteria, one is acid-producing bacteria, which catalyzes sorbose to gulonic acid through enzymes, commonly known as "acid production", but acid-producing bacteria cannot be cultured alone, and need to rely on another companion bacteria The existence of the pres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/60
Inventor 徐慧杨伟超吕淑霞张忠泽
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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