Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

An associated bacterium of the banana borer nematode with potential for genetic modification

A technology of banana perforating nematode and associated bacteria, which is applied in the directions of bacteria, nematicides, biocides, etc., can solve the problem of no biocontrol effect of banana nematode associated bacteria, and achieves strong genetic modification potential, high safety, and stable associated relationship. Effect

Active Publication Date: 2020-08-07
SOUTH CHINA AGRI UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no research report on the associated bacteria of banana nematodes and their biocontrol effects

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An associated bacterium of the banana borer nematode with potential for genetic modification
  • An associated bacterium of the banana borer nematode with potential for genetic modification
  • An associated bacterium of the banana borer nematode with potential for genetic modification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Acquisition of Pseudomonas fluorescens pf36, an associated bacterium of the banana borer nematode

[0032] 1. Isolation and purification of the bacteria associated with the banana borer nematode and the endophytic bacteria of the carrot callus

[0033] (1) Isolation of bacteria associated with the banana borer nematode

[0034] In order to ensure that the isolated bacteria have a stable companionship with the nematode banana, the tested 10 populations of the nematode nematode from different host plants (Table 1) were all subcultured on the carrot callus for more than 3 times. The subcultured banana perforator nematodes (mixed nematodes) were collected and placed in 1.5 ml sterilized centrifuge tubes (about 1000). Centrifuge at 4500rpm for 45s, discard the supernatant, wash the nematodes repeatedly 3 times, then grind the nematodes collected at the bottom of the tube thoroughly with a sterilized glass rod, add 1ml of sterile water and shake well, absorb 50μl o...

Embodiment 2

[0071] Example 2 Application of Pseudomonas fluorescens associated with banana nematode in the control of banana nematode

[0072] 1. Introduction of protease pase4 gene into bananas to wear the associated bacteria - Pseudomonas fluorescens

[0073] (1) Conversion method

[0074] The protease pase4 gene was introduced into the target bacteria Pseudomonas fluorescens pf36 through vector transformation and conjugation transposition respectively. The nucleotide sequence of the protease pase4 gene is shown in SEQ ID NO.1.

[0075] ① Vector transformation method

[0076] Using the recombinant plasmid pase4-pEASY connected with the protease pase4 gene preserved in the laboratory as a template, use the following primers to amplify the protease pase4 gene.

[0077] Upstream primer M1:

[0078]

[0079] The lower horizontal line is the EcoR I restriction site, and the wavy line is the ribosome binding site;

[0080] Downstream primer M2:

[0081]

[0082] The lower horizont...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a bacterium associated with the banana borer nematode with genetic modification potential. The associated bacteria are Pseudomonas fluorescens ( Pseudomonas fluorescens ) pf36, which was deposited in the Guangdong Microbial Culture Collection Center on November 8, 2017, with the preservation number GDMCC No: 60278. The present invention isolates and screens a dominant companion bacterium from multiple populations of the banana nematode, that is, the above-mentioned Pseudomonas fluorescens pf36, which has a very stable companion relationship with the banana nematode, and provides a source for the mediated expression of the nematode protease gene A genetically improved target bacterium is developed. Due to the associated relationship between the target bacterium and the nematode nematode, the target bacterium can be used for biological control of the nematode nematode after being improved to express the nematode protease gene, and it is safe for crops. The invention also provides a new idea and method for the prevention and control of plant nematodes, that is, the biological control of nematodes is realized by using the nematode companion bacteria to mediate the nematicidal protease gene.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to a bacterium associated with the nematode nematode punctatus with genetic modification potential. Background technique [0002] Plant nematode disease is a plant disease caused by the attack and parasitism of plant parasitic nematodes. Injured plants can affect normal growth and development due to invading nematodes absorbing nutrients in the body; secretions during nematode metabolism can also stimulate the cells and tissues of host plants, resulting in plant deformities, and control by natural enemies. Reduce the production and quality of agricultural products. Among them, Radopholus similis, also known as banana root rot, is the most dangerous obligate parasitic nematode in bananas, flower planting and many commercial crops. Its host range is very wide. There are more than 350 kinds of host plants reported. . In addition to serious harm to bananas, peppers and co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/21A01N63/27A01P5/00C12R1/39
CPCA01N63/00C12N9/50C12N1/205C12R2001/39
Inventor 谢辉王东伟陈德强丁善文其他发明人请求不公开姓名
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com