The invention provides a bacterial strain JF which is separated out from a large number of soils with mulching films used and can efficiently degrade phthalate, and belongs to the technical field of biology. The bacterial strain grows fast, can produce spore, is high in resistance to external environment, and reaches more than 90% of degradation rate regarding to phthalate within a broad range that the temperature is 30 to 45 DEG C and the pH (Potential of Hydrogen) is 5.0 to 8.0. The identification result shows that the bacterial strain is bacillus subtilis (Bacillus Subtilis), and the accession number is KF364634; and the bacterial strain is stored in China General Microbiological Culture Collection Center (CGMCC) located at No.3, Courtyard 1, West Beichen Road, Chaoyang District, Beijing, on July 22, 2013, with the storage number of CGMCC No.7950. The bacterial strain can be applied to bioremediation or biological purification of soil and sewage polluted by phthalate, and protects the ecological environment, and thus the safety of agricultural products is ensured.