Primer and probe combination and kit for detecting 12 kinds of children digestive tract infection related bacteria
A technology of digestive tract infection and primer probe, applied in the directions of microorganism-based methods, microorganisms, recombinant DNA technology, etc., to achieve the effect of prevention and treatment assistance, high accuracy and high sensitivity
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[0030] Use bacterial positive control as a sample. The kit is composed of primer probe mixed solution, RT-PCR Buffer, mixed enzyme, yang-PCR, and no RNase water; RT-PCR Buffer includes PCR buffer, DATP, DUTP, DCTP, DGTP, and MGCL 2 The mixed enzyme solution includes a HOT START TAQ enzyme, inverse transcriptase, and UNG enzymes; a plasmid containing the amplified gene sequence corresponding to the digestive pathogens in the yang nature control.
[0031] The primer in the amplification system was 100-1000 nm; the final concentration of the probe was 50-500 nm in the amplification system.
[0032] The reactant system of the kit is 30 μL, specifically: a nucleic acid template 7 μL, RT-PCR Buffer 20 μL, 1 μl of mixed enzyme solution and 2 μl of the primer probe mixture.
[0033] Operation and results of the kit
[0034] (1) Extract the sample DNA to the template (extracted from the child's feces), no RNase water, and 7 μL of the yang-nature control each additionally PCR reaction tube,...
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