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Method for improving 2-keto-L-gluconic acid production intensity through addition of betaine

A production intensity, betaine technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of suitable proportions, increased production intensity, and small pH fluctuations

Inactive Publication Date: 2015-10-21
DSM JIANGSHAN PHARMACEUTICAL (JIANGSU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report to increase the production intensity of 2-keto-L-gulonic acid by adding betaine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Put 6ml of the cultured common ketogenic cologne and Bacillus megaterium mixture into 40ml containing 8% sorbose, 0.5% corn steep liquor, 1.2% urea, 0.02% magnesium sulfate, 0.05% diphosphate Fermentation medium of potassium hydrogen, 0.5% calcium carbonate, and 0.1% betaine, the initial pH of the medium is 7.0, the culture temperature is 29°C, the volume of the shaker flask is 500ml, the rotation speed of the shaker is 210rpm, and the end point 2-KLG content of the fermentation broth is 79.16mg / ml , the fermentation period is 60 hours, and the production intensity is 1.32g / l·h. Compared with the control group, the fermentation period of the experimental group was shortened by 9.1%, and the yield and production intensity of 2-KLG increased by 2.87% and 14.2%, respectively.

Embodiment 2

[0029] Put 12ml of the cultured common ketogenic cologne and Bacillus megaterium into 80ml containing 8% sorbose, 0.5% corn steep liquor, 1.2% urea, 0.02% magnesium sulfate, 0.05% diphosphate Potassium Hydrogen. Fermentation medium with 0.5% calcium carbonate and 0.5% betaine, the initial pH of the medium is 7.0, the culture temperature is 31°C, the volume of the shaker flask is 750ml, the rotation speed of the shaker is 210rpm, the end point 2-KLG content of the fermentation broth is 79.72mg / ml, the fermentation cycle After 56 hours, the production intensity was 1.42g / l·h. Compared with the control group, the fermentation period of the experimental group was shortened by 15.2%, and the yield and production intensity of 2-KLG increased by 2.96% and 16.4%, respectively.

Embodiment 3

[0031] Put 12ml of the cultured common keto-based cologne and Bacillus megaterium into 80ml containing 8% sorbose, 0.5% corn steep liquor, 1.2% urea, 0.02% magnesium sulfate, 0.05% diphosphate Potassium Hydrogen. Fermentation medium with 0.5% calcium carbonate and 0.2% betaine, the initial pH of the medium is 7.0, the culture temperature is 31°C, the volume of the shaker flask is 750ml, and the rotation speed of the shaker is 210rpm. After 12 hours of fermentation, add 0.02% betaine every 4 hours to the end. The 2-KLG content at the end point of the fermentation broth was 79.95mg / ml, the fermentation period was 54 hours, and the production intensity was 1.48g / l·h. 4.29% and 19.1%.

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Abstract

The invention discloses a method for improving the production intensity of 2-keto-L-gulonic acid (2-KLG) by adding betaine, belonging to the technical field of vitamin C preparation through fermentation. A mixed bacteria system of ketogulonigenium vulgare and bacillus megaterium is adopted as a production strain, and 0.05-1.0% betaine is exogenously added into a fermentation medium as an osmotic pressure regulator so as to promote the cell growth and 2-KLG production and realize efficient production of 2-KLG. By adopting the process, the method disclosed by the invention can effectively shorten the fermentation period of 2-KLG and improve the production intensity.

Description

[0001] The patent application for the present invention is a divisional application. The application number of the original application is 201310693045.7, the application date is December 18, 2013, and the title of the invention is: a method for increasing the production intensity of 2-keto-L-gulonic acid by adding betaine. technical field [0002] The invention belongs to the technical field of vitamin C preparation by fermentation, and more specifically relates to a method for increasing the production intensity of 2-keto-L-gulonic acid by adding betaine. Background technique [0003] Vitamin C is an essential water-soluble vitamin that is involved in various metabolic activities. 2-keto-L-gulonic acid (2-keto-L-gulonic acid, hereinafter referred to as 2-KLG) is an important precursor for the synthesis of vitamin C. At present, major vitamin C manufacturers in China adopt the "two-step fermentation" production process. The key step of this technology is the second ferme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/60C12R1/11
Inventor 孔太胡少斌陈红刘杰
Owner DSM JIANGSHAN PHARMACEUTICAL (JIANGSU) CO LTD
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