Method for improving 2-keto-L-gluconic acid fermentation efficiency

A technology for production efficiency and gulonic acid, which is applied in the field of improving the fermentation production efficiency of 2-keto-L-gulonic acid and improving the two-step fermentation process of vitamin C, which can solve the problems of low fermentation production efficiency and so on

Inactive Publication Date: 2017-02-22
NINGXIA QIYUAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention is aimed at the problem of low production efficiency of the second step of fermentation in the existing vitamin C production process, and provides a production efficiency by improving the conversion of L-sorbose into 2-keto-L-gulonic acid, and then improving the production efficiency. Method for efficient production of 2-keto-L-gulonic acid fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. The steps of strain preparation and seed solution preparation are the same as those in the control example.

[0032] 2 fermentation:

[0033] 2.1 Fermentation tank optimized medium formula (%): urea 0.05-0.10, corn steep liquor 0.5-1.0, potassium dihydrogen phosphate 0.02-0.05, magnesium sulfate 0.01, calcium carbonate 0.05, pH 6.7-7.4.

[0034] 2.2 Small feed medium formula (%): sorbose 5.0, urea 0.1-0.2, corn steep liquor 2.0-5.0, potassium dihydrogen phosphate 0.02-0.05, calcium carbonate 0.05, pH 6.7-7.4.

[0035] 2.3 Sterilization method of small feed medium: L-sorbose is sterilized separately from other raw materials, cooled to below 40°C and mixed, and the initial sorbose concentration is 25-40mg / ml.

[0036] 2.4 Fermentation tank inoculation method and culture conditions: the same as the control example.

[0037] 2.5 Feeding during fermentation: add sorbose at the beginning of 0h of fermentation, and finish adding small ingredients within 10h. 35mg / ml, the...

Embodiment 2

[0040] 1. The steps of strain preparation and seed solution preparation are the same as those in the control example.

[0041] 2 fermentation:

[0042] 2.1 Fermenter optimized medium formula (%), small material medium formula (%) and sterilization method: same as Example 1.

[0043] 2.2 Fermentation tank inoculation method and culture conditions: the same as the control example.

[0044]2.3 Feeding during fermentation: add sorbose at the beginning of fermentation 0h, and add small feed in parallel within 12h. 12~35mg / ml, the residual sugar in the tank is lower than 0.6mg / ml, according to the concentration of L-sorbose in the fermentation medium, the end of adding L-sorbose 4~8 hours before putting in the tank; adding 20- 25% sterile sodium carbonate solution to adjust the pH value between 6.7 and 7.4.

[0045] 2.4 Fermentation end point: the concentration of 2-keto-L-gulonic acid is 133g.L-1, the fermentation period is 40h, the acid production rate is 3.33g.(L.h)-1, and the...

Embodiment 3

[0047] 1. The steps of strain preparation and seed solution preparation are the same as those in the control example.

[0048] 2 fermentation:

[0049] 2.1 Fermenter optimized medium formula (%), small material medium formula (%) and sterilization method: same as Example 1.

[0050] 2.2 Fermentation tank inoculation method and culture conditions: the same as the control example.

[0051] 2.3 Feeding during fermentation: add sorbose at the beginning of fermentation 0h, and add small feed in parallel within 15h. 12~35mg / ml, the residual sugar in the tank is less than 0.6mg / ml, according to the concentration of L-sorbose in the fermentation medium, the end of adding L-sorbose 4~8 hours before the tank; 25% sterile sodium carbonate solution to adjust the pH value between 6.7 and 7.4.

[0052] 2.4 Fermentation end point: the concentration of 2-keto-L-gulonic acid is 150.3g.L-1, the fermentation period is 40h, the acid production rate is 3.76g.(L.h)-1, and the sugar-acid conversi...

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Abstract

The invention relates to a method for improving 2-keto-L-gluconic acid fermentation efficiency. The method is characterized in that in the process of mixed strains fermenting 2-keto-L-gluconic acid, the mixed strains comprise ketogulonigenium vulgare and bacillus megaterium, seed solution cultured to the middle and later periods of logarithmic growth is inoculated into fermentation tank optimum culture media to ferment and culture, sorbose is fed since fermentation time is 0h, and small material culture media are fed at the prior period of the logarithmic growth. According to the method, production intensity of the 2-keto-L-gluconic acid can be improved, efficiency is improved, production capacity is optimized, the content of the 2-keto-L-gluconic acid in fermentation solution can reach 115.6-150.3mg / ml, acid producing rate is 2.89-3.76g.(L.h)-1, and the conversion rate of glucose acid is 87.2-95.0%.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for improving the production efficiency of 2-keto-L-gulonic acid fermentation, which is biased towards improving the vitamin C two-step fermentation process. Background technique [0002] Vitamin C two-step mixed bacteria fermentation system, including two fermentation steps, is called two-step fermentation method: the first step is to oxidize D-sorbitol into L-sorbose under the action of black acetic acid bacteria, commonly known as alcohol sugar conversion; The first-step fermentation process is mature and the conversion rate is high, which can reach more than 98%. At present, the research on its related genes and transformation substrates at home and abroad has been relatively clear; the second step is to further oxidize L-sorbose into 2-keto- L-gulonic acid, commonly known as sugar-acid conversion; the second step is the mixed fermentation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/60C12R1/11
CPCC12P39/00C12P7/60
Inventor 崔莉盛雪樊艳荣孙瑞君
Owner NINGXIA QIYUAN PHARMA
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