42results about How to "Shorten the lag period" patented technology

A gastrointestinal device is provided. The device includes a band sized and configured for residing in or around a pyloric sphincter region of the subject. The band is functional in maintaining the pyloric sphincter at a fixed opening size.

The invention provides a method for treating blue-green algae. The method includes the following steps of detecting a water body, placing padding and plants, mounting oxygenation devices, domesticating microorganisms, adjusting the water body, putting compound microorganisms, building an ecological system and strengthening treatment; wherein the water body to be treated is detected, pretreatment is conducted, a proper amount of water is taken to serve as habituated culture water for inhibiting external microbial communities for the algae, compound bacteria, enzymes and sugar are added to the habituated culture water, meanwhile, screening is conducted on the beneficial microorganisms three times to five times according to the existence condition of original indigenous and beneficial microorganisms of the water body, the screened beneficial microorganisms are extracted to be subjected to enlarge cultivation, the cultured composite microorganisms are put into the water body required to be treated, the composite microorganisms can destroy the blue-green algae structure, and accordingly, a proper growth breeding condition is provided for follow beneficial microorganisms and animals. According to the main mechanism of the method, the blue-green algae structure is mainly destroyed and utilized, and the method has the advantages of being advanced, reliable, safe, operable, low in investment and capable of generating the effect quickly and finally achieving the purpose that the water quality is purified.

The invention relates to nano-iron-loaded biochar, a preparation method of the nano-iron-loaded biochar, and an application of the nano-iron-loaded biochar in a dark fermentation hydrogen production process. Ferrite and soluble starch are taken as raw materials which are subjected to reaction under proper conditions and are dried and charred to obtain the nano-iron-loaded biochar, wherein the maincomponent of the nano-iron-loaded biochar is Fe2O3/C, and the particle diameter range of the nano-iron-loaded biochar is 2-150nm. The biochar is of an amorphous structure, and Fe2O3 is of a mesoporous structure. By adding the iron-loaded biochar to an anaerobic fermentation system, a synergistic strengthening effect of iron and biochar in the dark fermentation hydrogen production process is realized, so that the hydrogen yield and the process stability are improved. Fe2O3 can not only release iron ions to enhance the activity of fermentative hydrogen producing bacterium hydrogenase but also increase the transfer rate of electrons; and furthermore, the biochar in nano-particles can enrich microorganisms, improve the concentration of hydrogen producing bacteria, buffer acid accumulation andrelieve ammonia inhibition.

The invention discloses a fermentation process for producing pravastatin by transforming compactin by using actinomadura yumaense. According to the fermentation process based on the conventional fermentation process, the transformation ability of the microbial substrate and the fermentation level of the pravastatin are improved by adding trace element solution into a fermentation culture medium and utilizing the effect of the trace elements completely; the transformation ability of the microbial transformation bacteria-actinomadura yumaense on the compactin and the fermentation level of the pravastatin are effectively improved by controlling the culture temperature of the actinomadura yumaense and the transformation temperature of the compactin by using actinomadura yumaense; and the fermentation level is far higher than that of the prior art. The fermentation process is simple and convenient to operate, low in cost and suitable for large-scale production.

The invention discloses a fed culture method of hydrogen-producing bacteria and a biological enhancing method of a biological hydrogen producing system, which relates to a method for enhancing bacterial culture and the biological hydrogen producing system. The method solves the problems that the existing fermentation method has the defects of long startup time of a reactor, low hydrogen productivity and sludge loss during continuous operations of the biological hydrogen production. The method comprises the steps: A. hydrogen-producing bacteria are inoculated in nutrient solution in a fermentation tank for anaerobic fermentation; B. after the anaerobic fermentation, a part of fermentation solution is put into a biological hydrogen producing reacting device by a metering pump, and then fresh nutrient solution, which has the same volume as a part of fermentation solution put into the biological hydrogen producing reacting device, is added into the fermentation tank for ongoing anaerobic fermentation, thereby completing the fed culture of hydrogen-producing bacteria and the biological enhancement of the biological hydrogen producing system. The method shortens the startup time of the biological hydrogen producing reactor during the continuous operation to 15 days to 20 days, and increases the hydrogen productivity by 10 percent to 15 percent, thus avoiding the loss of active sludge.

The invention discloses a method for preparing a Hami melon vinegar beverage. The method comprises the steps of preparing Hami melon juice, expanding cultivation of active ferment, fermenting Hami melon wine, preparing a direct leavening agent, fermenting Hami melon vinegar and preparing the Hami melon vinegar beverage. The method combines a liquid submerged fermentation technology and a direct fermenting method and has the advantages of being quick in fermenting speed, short in fermenting period, high in raw material utilization ratio and acid producing efficiency and the like. The advantages of the liquid submerged fermentation technology are played to the most extent by controlling diameters including the leavening agent, fermentation liquor ingredients and the like. By adopting the direct leavening agent, the steps of earlier-stage activation, separation and purification, cultivation expanding and the like required by test tube acetic acid strains are omitted, a lag period of the acetic acid strains is shortened, and the overall fermenting process is shortened by more than 60 hours than that of the test tube strains. By controlling the process steps, functional ingredients of Hami melons are fully utilized, special flavor of the Hami melons is retained, and the beverage is gentle in sour, sweet, mellow, tasty and refreshing and enriches the fruit vinegar beverage market.

The invention discloses a culture medium and fermentation method capable of improving yield of corynebacterium glutamicum ectoines, and belongs to the field of bioengineering. The invention provides aformula of a fermentation culture medium. The fermentation culture medium is rich in a carbon source, a nitrogen source, inorganic salt and compound trace elements, which are required for growth of corynebacterium glutamicum CG-ECT3; a strain is enabled to rapidly grow, reproduce and be cultured for 13 hours; OD600 can reach over 40; a plasmid copy number is increased; and time of a lag period and time of a logarithmic phase are shortened. Meanwhile, according to the invention, by controlling residual sugar and a precusor substance L-asparaginic acid in fermentation liquor to be replenished in the fermenting process, the acid-producing ability of the recombinant corynebacterium glutamicum CG-ECT3 is improved, a fermentation period is shortened, and after fermentation is carried out for 45h, yield of the ectoines can reach over 48g/L.

An additive for synergistically promoting the proliferation and colonization of intestinal probiotics and its use method, which is composed of xylo-oligosaccharides, Clostridium butyricum and high fulvic acid sodium humate, and xylo-oligosaccharides provide nutrition for Clostridium butyricum , to promote the proliferation of Clostridium butyricum in the intestinal tract; Clostridium butyricum secretes vitamins and growth factors to promote the proliferation of probiotics, while secreting organic acids and antibacterial peptides to inhibit the growth of harmful bacteria; high fulvic acid sodium humate through the repair Intestinal mucosal damage can increase the level of probiotic adhesin receptors on the surface of intestinal mucosa, thereby promoting the colonization of probiotics in the intestinal tract. Through process coupling and functional synergy, the three can finally achieve the intestinal microecological balance of livestock and poultry and improve the intestinal tract. Barrier and absorption function, reduce diarrhea rate and improve feed utilization, thereby reducing the dependence on antibiotics in the breeding process.

The invention relates to a folium mori protein hydrolysate, a preparation method thereof and an enzyme beverage prepared from the folium mori protein hydrolysate. The preparation method of the foliummori protein hydrolysate comprises the following steps of preparing folium mori into folium mori slurry, and adding biological enzymes into the folium mori slurry for enzymolysis, wherein the biological enzymes comprise cellulase, pectinase and neutral protease. When the folium mori protein hydrolysate is used for microbial fermentation, the lag phase of fermentation microorganisms can be shortened, the logarithmic phase of the fermentation microorganisms can also be prolonged, and fermentation products with relatively high active ingredient content can be obtained under the condition of shortfermentation time.

The invention relates to application of MAL33 gene deletion in improving tolerance of saccharomyces cerevisiae to lignocellulose hydrolysate inhibitors, and belongs to the field of bioengineering. The invention provides an MAL33 gene deleted saccharomyces cerevisiae strain, and it is proved that compared with a control strain, the acetic acid tolerance of the deleted strain is greatly improved; the tolerance to other typical inhibitors and H2O2 in the lignocellulose hydrolysate is also improved; the delay phase in a glucose and xylose culture medium (YPDX) with 3.5 g/L acetic acid is shortened by 24 h, and the fermentation period of ethanol production by co-utilization of glucose and xylose is shortened by 20 h; the conditions of growth in a glucose and xylose culture medium (YPDX) containing a mixed inhibitor and ethanol production through co-fermentation of glucose and xylose are superior to those of a control strain; the invention provides an important method for improving the tolerance of the saccharomyces cerevisiae to the lignocellulose hydrolysate inhibitor, and provides a theoretical basis for overcoming the technical bottleneck in the production of second-generation fuel ethanol.

The invention provides a fermentation technology for improving the production level of recombinant collagen, and relates to the technical field of genetic recombination engineering bacterium fermentation. The fermentation technology for improving the production level of the recombinant collagen comprises the following steps of: S1, pichia pastoris engineering bacteria are inoculated into a seed tank to be cultured, glycerin and a supplemented medium are supplemented after dissolved oxygen is greatly increased, and when wet weight is increased to be larger than 100 g/L, the pichia pastoris engineering bacteria are transferred into a fermentation tank to start fermentation culture; S2, after the pichia pastoris engineering bacteria in the fermentation tank are cultured till the dissolved oxygen is greatly increased, a mixed carbon source material supplementation is carried out by using a carbon source culture medium and a supplemented medium, and the mixed carbon source material supplementation is stopped when the wet weight of the material is increased to be greater than 150g/L; and S3, after the carbon source is used up, methanol and the supplemented medium are added for carrying out induced expression until fermentation is finished. The fermentation technology disclosed by the invention can improve the expression quantity of the recombinant collagen and shorten a fermentation period, so that the fermentation production level of the recombinant collagen is improved, and the fermentation technology is suitable for stable industrial production.

The invention discloses an application of saccharomyces cerevisiae SNG1 gene deletion in improving vanillic aldehyde resistance. The gene sequence of the related SNG1 gene deletion mutant is composedof -18 to +203 bp nucleotide fragments of a saccharomyces cerevisiae SNG1 gene, nucleotide fragments of loxp-KanMX4-loxp and +1446 to +1644 bp nucleotide fragments of the saccharomyces cerevisiae SNG1gene, and the nucleotide sequence of the mutant is as shown in SEQ ID No.1. Experiments prove that under no stress, the wild type is not different from the growth of the sng1 delta mutant disclosed by the invention; however, under the stress of vanillic aldehyde, the maximum specific growth rate of the sng1 delta mutant is 70% higher than that of a control strain, and the specific consumption rate of vanillic aldehyde is 52% higher than that of vanillic aldehyde. It is prompted that the mutant strain is suitable for vanillin/alcohol production or construction of a high-vanillin-tolerance saccharomyces cerevisiae strain for producing second-generation fuel ethanol or other high-value compounds by taking lignocellulose as a raw material.

The invention provides a method for reinforcing leaching of bornite through high-iron sphalerite. The method includes the following steps that the high-iron sphalerite and the bornite are ground, andhigh-iron sphalerite powder and bornite powder are obtained; a 9K basic culture medium is subjected to high-temperature high-pressure steam sterilization, and the high-iron sphalerite powder and the bornite powder are intermittently sterilized; the sterilized high-iron sphalerite powder and the sterilized bornite powder are mixed, then the sterilized 9K basic culture medium is added, ore pulp is obtained, and then the pH value of the ore pulp is adjusted to 1.5-2.0; and the ore pulp is subjected to agitation leaching, and chemical conditions of a leaching solution are adjusted and controlled.The whole leaching period is shortened, meanwhile, the leaching rate and the leaching speed are greatly increased, and the method is clean and environmentally friendly, low in cost and suitable for large-scale application and popularization.

The invention provides a liquor-fermentation production method of neutral protease. The liquor-fermentation production method includes 1), culture activation, 2), seed cultivation, 3), liquor fermentation, and 4), extracting and refining of the neutral protease. The liquor-fermentation production method of the neutral protease has the advantages that through optimization of culture media and complete adoption of soluble culture media, the problems of large quantity of solid matters in existing culture media, high viscosity of the existing culture media, difficulty in after-treatment and high cost are solved; products with high enzyme activity are obtained through process optimization.

The invention discloses a method for efficiently producing carotenoid. The method comprises the steps that juice with the high carotenoid content is obtained by taking canned yellow peach leftovers as main raw materials through microwave color fixing, frozen-breaking and biological enzymolysis, and after the ingredients are regulated, a fermentation medium is obtained; fractional inoculation and temperature-variable fermentation are conducted on oceanic red yeast inoculated with the high-yield carotenoid, so that the oceanic red yeast is maximumly proliferated; after fermentation liquid is subjected to ultrahigh pressure homogenizing, vacuum centrifuging and demulsifcation separating, emulsion is obtained, and after the emulsion is extracted, separated and purified, the finished carotenoid is obtained, wherein the cell biomass of the fermentation liquid is 38-52 g/L, and the yield of the carotenoid is 47-61 mg/L. The preparation method is simple, the cycle is short, the efficiency is high, energy is saved, environmental friendliness is achieved, a low-temperature bio-processing technique is adopted in the whole process, the production cost is reduced, hidden environmental pollution danger is eliminated, a firm foundation is laid for sustainable development of the field of yellow peach deep processing technologies, and the positive economic and social development significance is achieved.