42results about How to "Shorten the lag period" patented technology

The invention discloses a fermentation process for producing pravastatin by transforming compactin by using actinomadura yumaense. According to the fermentation process based on the conventional fermentation process, the transformation ability of the microbial substrate and the fermentation level of the pravastatin are improved by adding trace element solution into a fermentation culture medium and utilizing the effect of the trace elements completely; the transformation ability of the microbial transformation bacteria-actinomadura yumaense on the compactin and the fermentation level of the pravastatin are effectively improved by controlling the culture temperature of the actinomadura yumaense and the transformation temperature of the compactin by using actinomadura yumaense; and the fermentation level is far higher than that of the prior art. The fermentation process is simple and convenient to operate, low in cost and suitable for large-scale production.

The invention relates to the field of microbial strains, in particular to a thermophilic anaerobic bacillus and a method for producing ethanol by using it. The bacterial strain of the present invention is Thermoanaerobacterium aotearoense P8G3#4, which has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation number is CGMCC NO.9000, and the preservation date is April 3, 2014 . The method of utilizing said bacterial strain to produce ethanol: first prepare the seed liquid of thermophilic anaerobic bacillus CGMCC9000, then transfer the thermophilic anaerobic bacillus CGMCC9000 seed liquid to the fermentation medium with 10-15% w / w inoculation amount, in Stirring culture under anaerobic conditions, and finally ethanol is separated from the fermentation broth. The strain has good genetic stability, stable yield traits, and the fermentation lag period is shortened to 1 / 4 of the original one, saving energy consumption.

The invention provides a culture medium of meningitis Neisseria, which is characterized in that the culture medium consists of the following components: 3.0-8.0g / L of sodium chloride, 0.05-0.2g / L of potassium chloride, 1.0-6.0g / L of disodium hydrogen phosphate, 5-20g / L of L-glutamic acid, 0.01-0.1g / L of L-arginine, 0.001-0.01g / L of L-tryptophan, 0.005-0.030g / L of L-aminothiopropionic acid, 0.01-0.1g / L of calcium chloride, 0.001-0.008g / L of ferrous sulphate, 0.1-1 / 0 g / L of magnesium sulfate, 5.0-20.0 g / L of soya peptone and 5.0-20.0g / L of glucose. The invention is good for the growth and the metabolism of bacterium, can obtain higher output, has a wide material source, is free of structural constituents of the other animals, can effectively remove exogenous interference, simplifies technology on manufacture, is easy to operate, can effectively amplify the meningitis neisseria, and satisfies the mass production of vaccines.

The present invention relates to MAL3 3 The application of gene deletion in improving the tolerance of Saccharomyces cerevisiae to lignocellulose hydrolyzate inhibitors belongs to the field of bioengineering. The present invention provides a strain MAL33 Saccharomyces cerevisiae strains with gene deletion have greatly improved tolerance to acetic acid; other typical inhibitors and H in lignocellulose hydrolyzate 2 o 2 The tolerance of the nutrient was also improved; the lag period in the 3.5 g / L acetic acid glucose-xylose medium (YPDX) was shortened by 24 h, and the fermentation cycle of ethanol production by co-utilization of glucose and xylose was shortened by 20 h; The growth and ethanol production of glucose and xylose co-fermentation in the glucose-xylose medium (YPDX) of inhibitors were better than those of the control strain; it provides a method for improving the tolerance of Saccharomyces cerevisiae to inhibitors in lignocellulose hydrolyzate , providing a theoretical basis for overcoming the technical bottleneck in the production of second-generation fuel ethanol.

The invention discloses a method for shortening the lagging period of lactic acid bacteria in the production of fermented milk, which is characterized in that it comprises the following steps: (1) adding part of the milk to be fermented into a batching tank, and adding oligosaccharides; (2) mixing and preparing Homogenize, filter, sterilize, and transport to the seed tank after cooling down; (3) add lactase and neutral protease for hydrolysis; (4) kill enzymes after hydrolysis is completed, add lactic acid bacteria powder after cooling, and stir until Bacteria recovery; (5) transport to the fermenter with a sterile centrifugal pump and mix and ferment with the milk to be fermented. The invention not only shortens the production time of fermented milk, but also increases the number of live bacteria in the final product and prolongs the survival time of lactic acid bacteria in the shelf life of the product.

The invention relates to an acetic fermentation expanding cultivation and acid steaming process and system. The process comprises the following steps that (1), pretreatment is conducted, wherein firstly, CIP alkaline wash and hot water wash are conducted in sequence on a first-level expanding cultivation tank, then wine vinegar is fed into the first-level expanding cultivation tank for acid steaming, the process conditions of the acid steaming are that the addition of wine vinegar is controlled to be one fifth of the volume of the tank and the total acid of the wine vinegar is not lower than 3%, the stirring speed of the first-level expanding cultivation tank is controlled to be greater than 730 rpm so as to ensure uniform distribution of steam in the tank, the wine vinegar is heated to be 90 DEG C and then lasts for 20 minutes, and the wine vinegar is discharged; (2), fruit wine is added in the pretreated first-level expanding cultivation tank, and then acetic acid bacteria seed fluid is added for first-level expanding cultivation. The wine vinegar is adopted for acid steaming on the expanding cultivation tank, the residual alkaline cleaning fluid is removed, the pH value in the expanding cultivation tank is reduced, the breeding environment of acetic acid bacteria is improved, the lag phase after seeds enter the new environment is shortened, and therefore the expanding cultivation period is shortened and the production cost is reduced.

The invention belongs to the technical field of agriculture microbes, and particularly relates to a type 2 streptococcus suis high-intensity fermentation medium and application. The type 2 streptococcus suis high-intensity fermentation medium is characterized by comprising the following components in bulking value by percentage: 0.1 to 5.0 percent of tryptone, 0.1 to 5.0 percent of yeast powder, 0.01 to 2.0 percent of ammonium acetate, 0.01 to 2 percent of MgSO4.7H2O, 0.1 to 4.0 percent of K2HPO4.3H2O, 0.01 to 1 percent of KH2PO4, and 0.01 to 2.0 percent of glucose, and also comprising 1 to 15 volume percent of bovine serum; and before sterilization, the pH of the medium is adjusted to be 7.4 to 7.8. The streptococcus suis high-intensity fermentation medium contributes to the growth metabolism of the type 2 streptococcus suis, thallus is high in growth speed, the large bacterium amount can be obtained, and the viable count of the cultivated type 2 streptococcus suis can reach 8.5 billion. Raw materials of the medium are wide in source; and the medium is low in cost, and can effectively amplify the type 2 streptococcus suis, and meet the requirement that type 2 streptococcus suis vaccine is produced in large scale.

The invention discloses a method for producing butyric acid through fermentation of a multilinked fiber bed bioreactor system. The method comprises the following steps: preparing a multilinked fiber bed bioreactor, activating a strain, culturing a seed liquid, performing pre-fermentation culture, and performing pre-fermentation batch fermentation or fed-batch fermentation. The method is simple in process; through the adoption of the method, on the one hand, large-scale industrial amplification can be realized at a relatively lower cost; on the other hand, a multi-phase mass transfer problem caused by simple volume amplification of the reactor and insufficiency of filler strength of a fiber bed can be avoided; the lag phase of a fermentation process can be shortened; the production cycle is greatly shortened; the concentration of a final product is improved; the subsequent separation of a fermentation liquid is facilitated; and detection and control of the fermentation process and equipment maintenance are facilitated.