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Type 2 streptococcus suis high-intensity fermentation medium and application

A high-density fermentation and Streptococcus suis technology, which is applied in the field of fermentation medium and its preparation, can solve the problems of not being completely suitable for the growth and metabolism of Streptococcus suis type 2, and achieve the effects of shortening production time, enriching nutrition, and increasing growth speed

Active Publication Date: 2013-10-02
WUHAN KEQIAN BIOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The TSB medium commonly used today, its main component is tryptone, in addition it contains 0.25% glucose, 0.25% K 2 HPO 4 And 0.5% NaCl, not fully suitable for the growth and metabolism needs of Streptococcus suis type 2

Method used

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  • Type 2 streptococcus suis high-intensity fermentation medium and application
  • Type 2 streptococcus suis high-intensity fermentation medium and application
  • Type 2 streptococcus suis high-intensity fermentation medium and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Culture medium components and ratio

[0022] Tryptone 28g; Yeast powder 27g;

[0023] Ammonium acetate 0.4g; MgSO 4 ·7H 2 O 0.25g;

[0024] K 2 HPO 4 ·3H 2 O 15g; KH 2 PO 4 1.5g;

[0025] Glucose 2.9g; Bovine serum 5%~15% (V / V);

[0026] Prepared by the following method:

[0027] A. Mix tryptone, yeast powder, ammonium acetate, MgSO 4 ·7H 2 O. After dissolving with distilled water, set the volume to 1000ml, adjust the pH to 7.7, and sterilize under high-pressure steam at 117°C for 20 minutes;

[0028] B. Weigh 100g of glucose, add distilled water to make up to 1000ml, and sterilize under high-pressure steam at 113°C for 20min;

[0029] C. Weigh 22.8g of dipotassium hydrogen phosphate trihydrate, add distilled water to make the volume to 100ml, and prepare dipotassium hydrogen phosphate solution. Weigh 13.6g of anhydrous potassium dihydrogen phosphate and add distilled water to make up to 100ml to make a potassium dihydrogen phosphate solution; take 84ml o...

Embodiment 2

[0033] Culture medium components and ratio

[0034] Tryptone 35g; Yeast powder 35g;

[0035] Ammonium acetate 0.5g; MgSO 4 ·7H 2 O 0.5g;

[0036] K 2 HPO 4 ·3H 2 O 20g; KH 2 PO 4 5g;

[0037] Glucose 5g; Bovine serum 15% (V / V);

[0038] Prepared by the following method:

[0039] A. Mix tryptone, yeast powder, ammonium acetate, MgSO 4 ·7H 2 O. After dissolving with distilled water, set the volume to 1000ml, adjust the pH of the culture medium to 7.8 before sterilization, and sterilize under high-pressure steam at 121°C for 25 minutes;

[0040] B. Weigh 100g of glucose, add distilled water to make up to 1000ml, and sterilize under high-pressure steam at 115°C for 25min;

[0041] C. Weigh 22.8g of dipotassium hydrogen phosphate trihydrate, add distilled water to 100ml, and prepare dipotassium hydrogen phosphate solution; weigh 13.6g of anhydrous potassium dihydrogen phosphate, add distilled water to 100ml, and prepare dibasic phosphate Potassium hydrogen phosphate so...

Embodiment 3

[0046] Culture medium components and ratio

[0047] Tryptone 15g; Yeast powder 15g;

[0048] Ammonium acetate 0.1g; MgSO 4 ·7H 2 O 0.1g;

[0049] K 2 HPO 4 ·3H 2 O 10g; KH 2 PO 4 1g;

[0050] Glucose 1g; Bovine serum 5% (V / V);

[0051] Prepared by the following method:

[0052] A. Mix tryptone, yeast powder, ammonium acetate, MgSO 4 ·7H 2 O. After dissolving with distilled water, set the volume to 1000ml, adjust the pH to 7.4, and sterilize under high-pressure steam at 115°C for 15 minutes;

[0053] B. Weigh 100g of glucose, add distilled water to make up to 1000ml, and sterilize under high-pressure steam at 110°C for 15min;

[0054] C. Weigh 22.8g of dipotassium hydrogen phosphate trihydrate, add distilled water to make the volume to 100ml, and prepare dipotassium hydrogen phosphate solution. Weigh 13.6g of anhydrous potassium dihydrogen phosphate and add distilled water to make up to 100ml to make potassium dihydrogen phosphate solution; take 84ml of dipotassi...

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Abstract

The invention belongs to the technical field of agriculture microbes, and particularly relates to a type 2 streptococcus suis high-intensity fermentation medium and application. The type 2 streptococcus suis high-intensity fermentation medium is characterized by comprising the following components in bulking value by percentage: 0.1 to 5.0 percent of tryptone, 0.1 to 5.0 percent of yeast powder, 0.01 to 2.0 percent of ammonium acetate, 0.01 to 2 percent of MgSO4.7H2O, 0.1 to 4.0 percent of K2HPO4.3H2O, 0.01 to 1 percent of KH2PO4, and 0.01 to 2.0 percent of glucose, and also comprising 1 to 15 volume percent of bovine serum; and before sterilization, the pH of the medium is adjusted to be 7.4 to 7.8. The streptococcus suis high-intensity fermentation medium contributes to the growth metabolism of the type 2 streptococcus suis, thallus is high in growth speed, the large bacterium amount can be obtained, and the viable count of the cultivated type 2 streptococcus suis can reach 8.5 billion. Raw materials of the medium are wide in source; and the medium is low in cost, and can effectively amplify the type 2 streptococcus suis, and meet the requirement that type 2 streptococcus suis vaccine is produced in large scale.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a fermentation medium suitable for large-scale cultivation of Streptococcus suis type 2 and its preparation method and application. technical background [0002] Streptococcus suis type 2 (Streptococcus suis) is the main bacterial pathogen that harms pigs (Lun ZR, 2007). It has 33 serotypes, of which Streptococcus suis type 2 (abbreviated as S.Suis 2) is the most common. A series of diseases, such as meningitis, arthritis, sepsis, endocarditis, serositis, miscarriage, abscess, were fatal. In addition, S. Suis 2 can also infect humans and cause meningitis (WertheimHF, 2009). Streptococcus suis type 2 has become a major problem in pig-raising countries, causing extremely serious economic losses to the pig industry. [0003] Although there are many studies on Streptococcus suis type 2 and some achievements have been made, there are few relevant reports on its culture,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/46
Inventor 陈关平陈守文何伟金梅林冀志霞徐高原陈波韩进尹争艳康超曹毅顾伟陈焕春
Owner WUHAN KEQIAN BIOLOGY CO LTD
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