71results about How to "Increase the amount of bacteria" patented technology

The invention discloses bacteria immobilization particles for water purification and a preparation method of the bacteria immobilization particles, and belongs to the technical field of water treatment. The preparation method comprises the following steps: preparing a bacteria concentrate through flocculation by utilizing activated carbon, diatomite and a chitosan acetic acid solution; adding diatomite, bentonite, silicon dioxide and a polyvinyl alcohol-sodium alginate mixed solution into the concentrate for stirring; carrying out granulation with a granulator to prepare the bacteria immobilization particles. The prepared bacteria immobilization particles are high in biological activity, high in bacteria content (5*10<9>-1*10<10> CFU/g), short in starting time, and high in reaction speed, has a honeycomb structure (100-300 m<2>/g in specific surface area) suitable for bacteria apposition growth and mass transfer, and can be used for immobilizing bacteria for long, prohibiting the invasion of external hazardous materials, and permanently and efficiently treating sewage in different environments and of different water quality types; meanwhile, industrialized production is available for a whole bacteria immobilization particle preparation process, and the prepared bacteria immobilization particles are high in mechanical property, long in service life, and convenient to store and convey.

This invention discloses Pediococcus acidilactici LH31 (CCTCC M207013), and a method for producing pediocin from it. The method comprises: inoculating Pediococcus acidilactici LH31 in modified MRS liquid medium, culturing, activating, inoculating activated Pediococcus acidilactici LH31 in a fermentation tank, and performing feed-batch fermentation with three stages. Pediococcus acidilactici LH31 is proliferated in stages 1 and 2. The maximal bacterial density (OD600 nm) can reach 30. Pediocin is accumulated in stages 2 and 3. The maximal titer can reach 18000 AU/mL. The method is suitable for industrialization.

The invention provides an aerobic denitrification reactor with a mycelium pellet as a carrier, which relates to the technical field of water treatment. The aerobic denitrification reactor comprises an enclosed reactor. An aeration head is arranged at the bottom the enclosed reactor; a water inlet pipe and a water outlet pipe are respectively arranged on an upper side surface and on a lower side surface of the enclosed reactor; a biomass carrier is provided in the enclosed reactor and is composed of mycelium pellets; a biofilm composed of aerobic denitrifying bacteria is formed on the mycelium pellets. The reactor provided by the invention enables nitrobacteria and aerobic denitrifying bacteria to well grow and propagate under the condition of a whole aerobic cycle and achieves a stable effect of synchronous nitrification and denitrification; the reactor utilizes the mycelium pellets as a carrier to overcome the problem of a short stable operation period of a whole system caused by considerable loss of the aerobic denitrifying bacteria since the aerobic denitrifying bacteria have weak competitiveness in the system and are difficult to form a dominant bacterial community, thus playing a crucial role in long-term stable operation of the whole reactor system.

An agricultural chemical for preventing and killing pine moth is prepared from Beauveria bassiana (CGMCC No.1573) through slant culture, culturing in jar, culturing in bag, and mixing with nutritive wet material. It has high effect (70-80%) and no pollution.

The invention discloses a high-density fermenting method of petroleum hydrocarbon degraded bacteria in the biological fermenting technical domain, which is characterized by the following: activating slope bacteria; enlarging the cultured seed liquid; preparing before inoculating; inoculating; supplementing the ferment culture medium batch by batch; adjusting the even growing speed of bacteria; reducing cost; simplifying the operation; producing large amount of petroleum hydrocarbon degraded bacteria within short period; solving the problem of soil pollution and the accident of petroleum pollution.

The invention discloses a preparation method of an immobilized microbial agent for kitchen waste treatment and an application thereof. The method comprises the following steps: respectively performing high-density liquid fermentation on Kluyer pichia yeast and geotrichum silvicola, and mixing the bacterial liquids in proportion; after concentrating a composite bacterial solution, adding the composite bacterial solution to a modified carrier, mixing the materials, adding a crosslinking agent during a mixing process, and then performing constant temperature aeration culture; and after constant temperature aeration, performing low temperature drying to obtain the immobilized microbial agent. The method of the invention not only significantly increases the amount of carrier immobilized thalline, but also makes the binding between the carrier and the thalline tight, prevents the living bacteria from being lost in the preparation process and the preservation, improves the live bacteria of the unit mass of the microbial agent, and reduces the amount of the microbial agent; The prepared microbial agent has strong capability for decomposing the kitchen waste, the odor generated in the process of degrading the kitchen waste can be eliminated, the amount of the thalline immobilized in the carrier is large, the binding between the carrier and the thalline is closer, and the biomass of the living bacteria is large.

The invention relates to a method and a device for joint denitrifying by denitrification and anaerobic ammonia oxidation embedding particles, and belongs to the field of sewage. The method comprises the following steps of respectively embedding a WPU (water-borne polyurethane) material by separated denitrification bacteria and anaerobic ammonia oxidation bacteria, putting the denitrification embedding particles into a flow-separated ball, putting anaerobic ammonia oxidation embedding particles into a Pall ring or into water in a floating state, and setting the denitrification embedding particles and the anaerobic ammonia oxidation embedding particles into an incomplete mixing state. The device comprises a water bath constant-temperature system, a sealing device, the flow-separated ball, the Pall ring and the like. The method and the device have the advantages that after embedding of the denitrification bacteria and anaerobic ammonia oxidation bacteria, the influence to the activity is avoided, the amounts of the denitrification bacteria and the anaerobic ammonia oxidation bacteria in the system are increased, the loss of bacteria amounts is reduced, the problems of slow growth of the anaerobic ammonia oxidation bacteria and stricter growth environment requirement in an anaerobic ammonia oxidation technology are solved, and the denitrifying efficiency of the system is improved by combining a nitrifying mode; the two types of embedding are set into the incomplete mixing state in the device, and respective feeding ratios can be flexibly adjusted according to the water quality.

The invention discloses a microorganic water-purifying slow release device, a preparation method and application thereof. The microorganic water-purifying slow release device comprises a protecting shell, a protective agent, a microbial flora powder and a multiplication accelerant; the protective agent, the microbial flora powder and the multiplication accelerant are contained in the protecting shell; the microbial flora powder and the multiplication accelerant are embedded in a timing slow-release layer. The microorganic water-purifying slow release device provided by the invention can maintain higher level for a long time and can keep ammonia nitrogen, nitrite and nitrate concentration at lower state, and the microorganic water-purifying slow release device need not be put in for the second time after being put in for a long period of time, so that a great convenience in use can be brought.

The invention relates to an acidithiobacillus caldus gene engineering strain and applications thereof. The acidithiobacillus caldus MTH-04(pJRD215-tac-sor) gene engineering strain is preserved in the general microbiology centre of China Committee for Culture Collection of Microorganisms on June 28, 2013, address: Institute of Microbiology Chinese Academy of Sciences, NO.3, NO.1 yard, beichen west road, Beijing chaoyang district. The strain preservation number: CGMCC NO.7388. Compared with the acidithiobacillus caldus MTH-04, the acidithiobacillus caldus MTH-04(pJRD215-tac-sor) gene engineering strain has the advantages that the capability of sulfur oxide is enhanced, and the bacteria biomass, sulfur and oxygen reductase enzyme activity and the transcriptional level of key enzyme of a sulfur oxidation system are increased.

The invention belongs to the technical field of agricultural microbes and specifically to isolation and screening as well as fermentable cultivation of a streptococcus suis 7-type strain. A streptococcus suis 7-YZ strain with a preservation number of CCTCC NO: M2011160 is obtained through separation. The fermentation technology comprises the steps of: 1) taking streptococcus suis 7-YZ strain cant seeds for streak cultivation on an TSA culture medium flat panel to obtain first-class seeds; 2) grafting first-class seed single colony into a TSB (Trypticase Soy Broth) culture medium shake flask to enable the OD600 to be 0.7-0.9 so as to obtain second-class seeds; 3) grafting the second-class seeds to the shake flask for fermentable cultivation so as to obtain a streptococcus suis 7-YZ bacteria liquid. According to the streptococcus suis 7-type high-density fermentation medium and the special strain, disclosed by the invention, growth and metabolism of streptococcus suis 7-YZ strain are facilitated, fast growth speed of the thallus is achieved, relatively high bacteria amount can be obtained, 6-7 billions of the streptococcus suis 7-YZ viable counts are cultured. The production cost is decreased by 30-50% when being compared with that of a commercial TSB culture medium.

The invention relates to a method and a device of nitrifying domestic wastewater by embedded nitrobacteria, and belongs to the field of sewage treatment. The method comprises the following steps: separating and enriching separated nitrobacteria and embedding by a WPU material; placing the embedded nitrobacteria in a special nitrifying reactor device which comprises a water bath constant temperature system, aerating equipment, stirring equipment, a nitration reaction tank and the like. Due to the embedded nitrobacteria, the hydraulic retention time and the biological retention time are separated, and infinitely long biological retention time is realized, so that the biomass of nitrobacteria in the reactor is greatly increased, and the wastewater treatment efficiency is improved. Meanwhile, the reactor has the advantages of high impact load, easiness in recovery start and no mud discharged, and the dosage of the embedded nitrobacteria carriers can be agilely adjusted according to the water quality of the source of water.

The invention relates to a Saccharomyces cerevisiae strain, a method for producing high-nucleic acid yeast and an application thereof. The invention provides a strain of Saccharomyces cerevisiae d8.8,which is deposited in China Typical Culture Collection Center and has the accession number of CCTCC NO: M 2017148. At the same time, the invention also provides a microbial agent obtained by fermenting the strain and a method for producing yeast. The method comprises shake flask culture, seed culture, primary fermentation, primary fermentation, secondary fermentation and commercial fermentation of strain d8.8, respectively, so that the final yeast concentration is 180-240g/L. As that yeast strain is utilized for industrialized fermentation production, the nucleic acid content in the yeast body reach more than 20% of the dry weight of the yeast body, the nucleic acid content in the yeast is increased, and the industrialized production cost is reduced.

The invention provides the extract of Rhus typhina fruit and a preparation method thereof, and application of the extract to fermentation production of genetic engineering products, belonging to the technical field of processing of agricultural products. The method comprises the following steps: mixing dried Rhus typhina fruit with an extraction solution so as to obtain a mixed material, wherein the extraction solution is an aqueous solution of ethanol; subjecting the mixed material to heating reflux under a slight boiling state, then carrying out cooling to room temperature and carrying out filtering and centrifugation so as to obtain clear liquid; and subjecting the clear liquid to pressure-reduced concentration according to a volume ratio of 8-12: 1 and carrying out degerming so as to obtain the extract of Rhus typhina fruit, i.e., RtSE. The extract RtSE can increase the amount of host bacteria in a genetic engineering fermentation system; when only RtSE is used in the fermentation system, the biomass of host cells in the fermentation system is increased by 5% or above, and a total protein amount and the active expression quantity of exogenous proteins are both increased by 20% or above; and when RtSE and IPTG are both used in the fermentation system, the biomass of the host cells in the fermentation system is increased by 12% or above, the total protein amount is increased by 30% or above, and the active expression quantity of the exogenous proteins are increased by 35% or above.

The invention provides a fermentation method of L-lysine. According the invention, nutrient division fermentation and high-density fermentation are combined, a fermentation medium is prepared according to a ratio of 4: 6, 40% as a fermentation base material and 60% into a concentrated fermentation medium, fed batch is started 2 h after the beginning of fermentation and stopped 4 h before the end of fermentation, and the concentration of the initial fermentation medium is reduced to prevent the excessive osmotic pressure of bacteria due to excessive nutrition, which inhibit the growth of the bacteria. Nutrient division fermentation started 2 h after the beginning of fermentation makes up for nutrient deficiency and imbalance in the bacteria growth process, so that the bacteria activity is improved, the bacteria density in fermentation liquor is increased, the bacteria amount is increased by about 20%, the fermentation period is shortened to 40 h from 44 h, and the cost is reduced; in the fermentation process, balanced nutrition supplementation enables metabolic flow to flow to the L-lysine more, the conversion rate is increased from 70% to 73%, generation of byproducts such as acetic acid and alanine is effectively reduced, and the yield of the L-lysine is increased from 223 g/L to 261 g/L.

The invention belongs to the technical field of microbial fermentation product extraction, and particularly relates to a method for efficiently extracting glucosamine from fermentation broth. On the basis of a method of extracting glucosamine with cation exchange resin in the prior art, a buffer solution of equilibrium activated resin is improved to effectively improve the adsorption efficiency ofthe resin to glucosamine, the saturated adsorption time of the resin is effectively shortened, the time of adsorption treatment is shortened, and the adsorption efficiency is effectively improved. Atthe same time, by improving an eluent in the existing method, glycine can effectively promote the connection of an amino group and the resin and helps to elute the target product, glucosamine, so asto effectively improve the efficiency of desorption process and improve the extraction rate of glucosamine in the fermentation broth on the whole.

The invention relates to a method for high-density culture of bacillus subtilis. The method comprises the steps of (1) carrying out three-level seed activation by regarding bacillus subtilis as fermentation bacteria, wherein a activation culture medium comprises the components: 10g/L of peptone, 3g/L of beef powder, 5g/L of sodium chloride and 1g/L of glucose; (2) inoculating activated bacteria to a fermentation culture medium for culture, wherein the fermentation culture medium comprises the components: 10g/L of glucose, 15g/L of soybean cake powder, 0.3g/L of MnSO4, 5g/L of NaCl, 0.3g/L of K2HPO4, 5g/L of calcium carbonate; fermenting the components at a temperature of 30 DEG C under a stirring rotating speed of 200r/min for 48h to obtain high-density cultured bacillus subtilis. By improvement of culture medium prescriptions and fermentation conditions, viable count of bacillus subtilis is more than 8.9*10<9>cfu/mL, necessary conditions are provided for industrial fermentation of bacillus subtilis, and rate of production can be increased greatly.

The invention relates to a novel milk cow forage zinc-source additive adopting super-high chelation strength organic zinc, in particular to an application of the super-high chelation strength organic zinc in the novel zinc-source additive of the milk cow forage. Compared with the conventional zinc-source additive (mainly inorganic zinc sulfate and zinc dioxide), the novel super-high chelation strength organic zinc provided by the invention has the advantages that the existing form of the zinc is more similar to the existing form of the zinc element in a milk cow body, and the bioactivity is higher. Therefore, the health condition of the milk cow can be obviously improved due to the selection of the novel super-high chelation strength organic zinc in the preparation process of a milk cow total mixed ration, the milk production performance and the milk quality of the milk cow are improved, and the efficient and sustainable development of dairy industry is powerfully promoted.

The invention discloses a gene expression cassette, which is constituted by sequentially connecting a promoter, a nucleotide sequence shown as SEQ ID NO.1 and a terminator sequentially, an expressionvector which is interpolated into the gene expression cassette and two multiple-stress-resistance yeast strains which are prepared via the gene expression cassette and are high in cell viability. According to the technical scheme, saccharomyces cerevisiae is transformed by virtue of the gene expression cassette of the invention, so that the fine transformation of ubiquitin proteasome is achieved;the invention provides a method which is capable of simultaneously improving cell viability and multiple stress resistance of the saccharomyces cerevisiae and an application of the method; and the obtained saccharomyces cerevisiae engineering strain is stable in genetic performance, and has a broad application range and a broad application prospect in industrial fermentation.

The invention belongs to the technical field of amino acid fermentation, and discloses a total-nutrient feeding high-density fermentation method for producing L-glutamic acid by fermenting temperature-sensitive corynebacterium glutamicum. The total-nutrient feeding high-density fermentation method comprises the steps of: inoculating the temperature-sensitive corynebacterium glutamicum into fermentation liquor, and continuously feeding a total-nutrient culture medium into the fermentation liquor when the temperature-sensitive corynebacterium glutamicum grows to a logarithmic phase, wherein the total-nutrient culture medium comprises inorganic salt ions such as sylvite, magnesium salt and the like and various growth factors required for growth of other temperature-sensitive corynebacterium glutamicum. According to the method, the total-nutrient culture medium is fed at the logarithmic phase of thalli, and the fed total-nutrient culture medium can timely make up nutrient substances consumed by growth and production of the thalli, so that the thalli are always in an optimal fermentation environment, and the thalli density in the fermentation liquor is improved; and meanwhile, in the later period of fermentation, feeding of the total-nutrient culture medium also can improve the activity of the thalli and ensure the production performance of the thalli so as to improve the yield of the L-glutamic acid.

The invention discloses a method for conducting synergistic bio-drying with an auxiliary material and a return material. Kitchen waste is dried through a bio-drier, and the kitchen waste with the machine handling capacity being 70%-80% is added into the bio-drier, and the balance is the auxiliary material. The auxiliary material comprises 40%-80% of saw dust, and the balance is the return material. The return material refers to a material left in the previous batch of bio-drying and contains 15%-25% of saw dust, the balance is the kitchen waste and the moisture content is 10%-20%. By adoptionof the method, the return material is recovered and reconstituted into the auxiliary material with the saw dust to be subjected to synergistic bio-drying, the bacterium quantity in a pile body can beeffectively increased, the temperature of the pile body can be better increased, the moisture content of the pile body can be lowered more quickly, and energy consumption is reduced while quick synergistic bio-drying is achieved.

The invention provides a method for producing long-chain dicarboxylic acid through biological fermentation, which adopts candida tropicalis or candida vesii as a fermentation microorganism, and comprises the following steps: 1) culturing the microorganism by adopting a two-stage seeding tank, the thallus growth density (OD620) after the culture of the first-stage seeding tank is greater than 0.5, and the thallus growth density (OD620) after the culture of the second-stage seeding tank is greater than 0.5; and (2) inoculating the bacterial liquid into a fermentation tank for fermentation after the culture in the secondary seed tank is finished, and controlling the pH value within the range of 6.8-7.1 during the fermentation period. The novel method disclosed by the invention is short in fermentation period, large in alkane addition amount, high in acid yield and high in conversion rate, and meets the production requirements of energy conservation, emission reduction and cost reduction.