Culturing method for improving ammoxidation capability of nitrobacteria

A technology for nitrifying bacteria and culturing methods, applied in microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, etc., can solve the problems of increasing difficulty in nitrifying bacteria culturing, large environmental factors, and slow growth. , to achieve the effect of promoting rapid proliferation, solving growth inhibition and enhancing vitality

Inactive Publication Date: 2010-08-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Nitrifying bacteria is a kind of chemical energy inorganic autotrophic bacteria, which can only use inorganic medium for growth and reproduction. It has the disadvantages of slow growth, large oxygen demand, and great influence of environmental factors, which makes the cultivation of nitrifying bacteria more difficult.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Dissolve 1.0g of sodium bicarbonate, 0.5g of ammonium sulfate, 0.3g of sodium carbonate, 0.1g of sodium chloride, 0.1g of potassium dihydrogen phosphate, 0.1g of magnesium sulfate, 0.01g of ferrous sulfate and 0.1mL of trace elements (FeCl) in 1000mL of water. 3 ·6H 2 O 1.5g / L, H 3 BO 3 0.15g / L, CuSO 4 ·5H 2 O 0.03g / L, KI 0.18g / L, MnCl 2 4H 2 O 0.12g / L, Na 2 MoO 4 2H 2 O 0.06g / L, ZnSO 4 7H2O 0.12g / L, CoCl 6 ·H 2 O 0.15g / L and disodium EDTA 10g / L. ) to make a culture medium for nitrifying bacteria, adjust the pH to 6.8 with 0.1M HCl, and add tourmaline with a particle size of 0.1 μm to the culture medium at a mass ratio of 0.5%. The nitrifying bacteria were mixed at a volume ratio of 10% (OD 600 0.5) inoculum amount was inoculated into the culture medium, and cultured for 7 days at 28°C and 4.0 mg / L dissolved oxygen. After 7 days, the cell mass and ammonia oxidation capacity of the nitrifying bacteria were detected, and the results were compared with the ...

Embodiment 2

[0023] Dissolve 4.0g of sodium bicarbonate, 1.2g of ammonium sulfate, 0.8g of sodium carbonate, 0.5g of sodium chloride, 0.5g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, 0.05g of ferrous sulfate and 1.0mL of trace elements (FeCl) in 1000mL of water. 3 ·6H 2 O 1.5g / L, H 3 BO 3 0.15g / L, CuSO 4 ·5H 2 O 0.03g / L, KI 0.18g / L, MnCl 2 4H 2 O 0.12g / L, Na 2 MoO 4 2H 2 O 0.06g / L, ZnSO 4 7H2O 0.12g / L, CoCl 6 ·H 2 O 0.15g / L and disodium EDTA 10g / L. ) formula to make a culture medium for nitrifying bacteria, adjust the pH to 8.0 with 0.1M HCl, and add tourmaline with a particle size of 0.5 μm to the culture medium at a mass ratio of 5%. The nitrifying bacteria were mixed at a volume ratio of 15% (OD 6000.5) was inoculated into the culture medium, and cultured for 7 days at 34°C and 4.0 mg / L dissolved oxygen. After 7 days, the cell mass and ammonia oxidation ability of nitrifying bacteria were detected, and the results were compared with the cell mass and amm...

Embodiment 3

[0025] Dissolve 2.0g of sodium bicarbonate, 1.0g of ammonium sulfate, 0.4g of sodium carbonate, 0.3g of sodium chloride, 0.3g of potassium dihydrogen phosphate, 0.3g of magnesium sulfate, 0.03g of ferrous sulfate and 0.5mL of trace elements (FeCl) in 1000mL of water. 3 ·6H 2 O 1.5g / L, H 3 BO 3 0.15g / L, CuSO 4 ·5H 2 O 0.03g / L, KI 0.18g / L, MnCl 2 4H 2 O 0.12g / L, Na 2 MoO 4 2H 2 O 0.06g / L, ZnSO 4 7H2O 0.12g / L, CoCl 6 ·H 2 O 0.15g / L and disodium EDTA 10g / L. ) formula to make a culture medium for nitrifying bacteria, adjust the pH to 7.4 with 0.1M HCl, and add tourmaline with a particle size of 0.8 μm to the culture medium with a mass ratio of 2%. The nitrifying bacteria were mixed at a volume ratio of 12% (OD 600 0.5), and cultivated for 7 days at 30°C and 4.0 mg / L dissolved oxygen. After 7 days, the cell mass and ammonia oxidation ability of nitrifying bacteria were detected, and the results were compared with the cell mass and ammonia oxidation capacity of nitrif...

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Abstract

The invention discloses a culturing method for improving the ammoxidation capability of nitrobacteria, which comprises the following steps: dissolving 1.0-4.0g of sodium bicarbonate, 0.5-1.2g of ammonium sulfate, 0.3-0.8g of sodium carbonate, 0.1-0.5g of sodium chloride, 0.1-0.5g of monopotassium phosphate, 0.1-0.5g of magnesium sulfate, 0.01-0.05g of ferrous sulfate and 0.1-1.0mL of trace element into 1,000mL of water; feeding tourmaline at the mass percentage of 0.5-5 percent into a culture medium; inoculating nitrobacteria at the inoculation quantity with the volume percentage of 10-15 percent to a culture medium in which the tourmaline is added; and culturing for 7-10 days under the condition that the temperature is 28-34DEG C and the dissolved oxygen quantity is not less than 2.0mg / L. The invention ensures that the growing metabolism velocity of the nitrobacteria is remarkably accelerated and the average degradability of the nitrobacteria is improved by 50-120 percent as comparison with the conventional culture medium as well as has the characteristics of simple process, good repeatability and remarkable effect.

Description

technical field [0001] The invention relates to a method for cultivating nitrifying bacteria, in particular to a culture medium and culture conditions capable of improving the ammonia oxidation ability of nitrifying bacteria. Background technique [0002] Due to the continuous development of the economy and society, people's demand for aquatic products is increasing, prompting the rapid promotion of high-density aquaculture, resulting in the deterioration of the aquaculture water environment and surrounding waters. The serious damage to the micro-ecological environment of the water body has exacerbated the excessive accumulation of ammonia and nitrite in large areas and the frequent outbreak of aquatic organism poisoning incidents, which directly or indirectly caused huge economic losses in our country. [0003] In view of the above problems, most of the treatment methods currently used are chemical and physical treatment methods, but these methods have disadvantages such as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12R1/01
Inventor 林炜铁罗剑飞
Owner SOUTH CHINA UNIV OF TECH
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