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Culture method of high-bacteria-amount fermentation liquor of brevibacillus laterosporus

A technique for short spores of lateral spores and a culture method, which is applied in the field of microorganism preparation, can solve the problem of low bacterial content in products, and achieve the effects of increasing the amount of spores, increasing the content of viable bacteria, and increasing the amount of bacteria

Pending Publication Date: 2020-04-07
CHANGYI MINGXING FEED CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to provide a kind of culture method of the high-bacterial quantity fermented liquid of Brevibacillus lateralosporus, to solve the problem that the product bacteria content is not high to the deficiency of existing Brevibacillus lateralsporogen fermentation technology, to improve product activity Bacteria content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The strain used in this example is a strain of Brevibacillus Laterosporu bacterium (Brevibacillus Laterosporu), which was bred from cotton root soil according to the conventional dilution plate separation method.

[0030] The method for cultivating the high bacterial count fermented liquid of Brevibacillus spp. involved in the present embodiment comprises the following processing steps:

[0031] (1) Preparation of bacterial strain liquid medium: 5 gram glucose, 10 gram sodium chloride, 10 gram peptone, 5g yeast extract powder and 1 liter of water (pH value is 7.0), prepare three parts;

[0032] The preparation of seed rejuvenation liquid medium: 5 grams of glucose, 5 grams of sodium chloride, 5 grams of peptone, 5 grams of yeast extract powder and 1 liter of water (pH value is 7.0), prepare a portion;

[0033] Add 1.5% agar to a portion of the above bacterial culture liquid culture medium, pour it into a sterile test tube to make a solid slant medium for seeds.

[0034...

Embodiment 2

[0048] The strain used in this example is a strain of Brevibacillus Laterosporu bacterium (Brevibacillus Laterosporu), which was bred from tomato root soil according to the conventional dilution plate separation method.

[0049] The method for cultivating the high bacterial count fermented liquid of Brevibacillus spp. involved in the present embodiment comprises the following processing steps:

[0050] (1) Preparation of bacterial strain liquid medium: 5 gram glucose, 10 gram sodium chloride, 10 gram peptone, 5g yeast extract powder and 1 liter of water (pH value is 7.0), prepare three parts;

[0051] The preparation of seed rejuvenation liquid medium: 5 grams of glucose, 5 grams of sodium chloride, 5 grams of peptone, 5 grams of yeast extract powder and 1 liter of water (pH value is 7.0), prepare a portion;

[0052] Add 1.5% agar to a portion of the above strain liquid culture medium and pour it into a sterile test tube to make a solid slant medium for seeds;

[0053] Add 1....

Embodiment 3

[0067] The strain used in this example is a strain of Brevibacillus Laterosporu bacterium (Brevibacillus Laterosporu), purchased from the Guangdong Microbial Culture Collection Center, and the strain number is GDMCC 1.404.

[0068] The method for cultivating the high bacterial count fermented liquid of Brevibacillus spp. involved in the present embodiment comprises the following processing steps:

[0069] (1) Preparation of bacterial strain liquid medium: 5 gram glucose, 10 gram sodium chloride, 10 gram peptone, 5g yeast extract powder and 1 liter of water (pH value is 7.0), prepare three parts;

[0070] The preparation of seed rejuvenation liquid medium: 5 grams of glucose, 5 grams of sodium chloride, 5 grams of peptone, 5 grams of yeast extract powder and 1 liter of water (pH value is 7.0), prepare a portion;

[0071] Add 1.5% agar to a portion of the above strain liquid culture medium and pour it into a sterile test tube to make a solid slant medium for seeds;

[0072] Add...

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Abstract

The invention belongs to the technical field of microorganism preparation, and particularly relates to a culture method of high-bacteria-content fermentation liquor of brevibacillus laterosporus. Thebrevibacillus laterosporus original starting strain can be a commercially available brevibacillus laterosporus original starting strain, and can also be a brevibacillus laterosporus strain bred from plant root systems, root system soil and the like according to an existing conventional dilution plate separation method. According to the invention, brevibacillus laterosporus strains are subjected toslant activation and seed rejuvenation and then inoculated into a fermentation medium with unique design, fermentation seeds with high activity are obtained through subsection control culture, and then fermentation is carried out through a fermentation formula and a fermentation process with unique design, so that the viable bacteria content and the spore bacteria amount of the fermentation liquor are effectively improved. The bacteria amount of the brevibacillus laterosporus fermentation liquor cultured by the method can reach 200*108 CFU / mL, and the bacteria amount of the product is remarkably increased.

Description

technical field [0001] The invention belongs to the technical field of microorganism preparation, and in particular relates to a method for cultivating a high-bacteria-amount fermented liquid of Brevibacillus lateralosporus. Background technique [0002] Brevibacillus Laterosporu (Brevibacillus Laterosporu) belongs to the genus Bacillus and is a natural strain approved by the Microbiology Testing Center of the Ministry of Agriculture. Its spores are oval, lateral, and the cysts are enlarged. One side of the free spores is thicker than the other. . Laterospores have toxic effects on invertebrates, including the larvae of various mosquitoes, beetles, and nematode eggs, etc. The toxic substances are mainly spore-like inclusions. Laterospora can secrete a variety of antibacterial substances, mainly including non-peptide bactosporine, peptide antibiotics, etc., and can also produce protease, chitinase, lysozyme and other enzymes. Therefore, it can be used to prevent and control ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/08
CPCC12N1/20
Inventor 邱彦国田相利张艾青陈永科雷勇王福强苏成文姜八一
Owner CHANGYI MINGXING FEED CO LTD
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