Production method of fermented feed rich in bifidobacterium lactis

Abstract

The invention belongs to the field of biological feeds, and particularly relates to a production method of a fermented feed rich in bifidobacterium lactis. The method includes the steps of activating strains to obtain a fermentation seed solution of bacillus subtilis, saccharomyces cerevisiae, the bifidobacterium lactis, bacillus coagulans and lactobacillus paracasei; and mixing soybean meal with bean dregs and wheat middlings to obtain a fermentation culture medium, and performing anaerobic fermentation under a sealed condition to obtain a finished product. According to the production method of the fermented feed rich in the bifidobacterium lactis, a mixed bacteria anaerobic fermentation mode is adopted, the bacillus subtilis, the saccharomyces cerevisiae and the bacillus coagulans are used for consuming residual oxygen in the environment at the initial stage of fermentation, the lactobacillus paracasei is rich in acid production, and environment pH suitable for growth is provided for the bifidobacterium lactis. Through the symbiotic synergistic effect of strains, the thallus density is increased, fermentation is thorough, the amount of the bifidobacterium lactis is high, the content of organic acid is rich, the palatability is good, and the economic benefit is high. A qPCR method is used for detecting the number of the bifidobacterium lactis in the fermented feed, so that the detection time is short, and the sensitivity is high.

Description

technical field [0001] The invention belongs to the field of biological feed, and in particular relates to a production method of fermented feed rich in bifidobacterium lactis. Background technique [0002] Bifidobacteria are anaerobic probiotics and an important part of the intestinal flora of animals. They can decompose sugars and produce acids such as acetic acid and lactic acid; Binding and adhering to the surface of the intestinal mucosa to form a biofilm, which plays a protective role in occupying space, and has the functions of stabilizing the intestinal environment, resisting harmful bacterial infection, and promoting digestion. Bifidobacteria can better utilize stachyose in soybean meal and degrade anti-nutritional factors in raw materials. However, Bifidobacteria are more susceptible to factors such as oxygen, pH value, and storage temperature than other bacteria. The existence of different strains in the fermentation substrate will also affect the growth of Bifid...

AI Technical Summary

Problems solved by technology

Neither of these two published invention patents added strains rich in digestive enzymes such as Bacillus subtilis, Bacillus coagulans, and yeast, and the substrate metabolism is not complete. At the same time, because there may be residual oxygen in the fermentation environment, the lack of aerobic bacteria will affect anaerobic bacteria. Create an oxygen environment, inhibit the growth of bifidobacteria, and reduce the quality of fermentation

Existing research reports did not detect changes in the amount of Bifidobacteria in fermented feed

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