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Method for producing long-chain dicarboxylic acid through biological fermentation

A long-chain dibasic acid and biological fermentation technology, applied in the field of biological fermentation to produce long-chain dibasic acid, can solve the problems of low acid production rate, insufficient efficiency, long fermentation time and the like

Inactive Publication Date: 2021-05-28
青岛智库生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of long-chain dibasic acid production by biological method in the prior art, the production fermentation time is long, the efficiency is not high enough and the acid production rate is low, the present invention provides a new method for producing long-chain dibasic acid by biological fermentation

Method used

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Embodiment 1

[0030] The method for producing long-chain dibasic acid by biological fermentation of the present embodiment comprises the following steps:

[0031] (1) First-class seed tank culture: alkaline water boiling tank: pH≈12, 120℃, 4h; empty elimination: 130℃, 1h; actual elimination: adjust pH properly before elimination, so that the pH before seeding is in the range of 6.7~7.0 . The temperature is 121-123°C, the temperature is kept for 30 minutes, and the volume after the actual elimination is 1.4-1.6m 3 After the actual elimination, the temperature was rapidly lowered, and when the temperature dropped to 30 °C ± 0.5 °C, Candida tropicalis was used for inoculation and culture.

[0032] In the first-level culture process, normal culture in the early stage, when the dissolved oxygen is close to 40%, increase the air volume to ensure that the dissolved oxygen is maintained at 40%-50%, and the air volume is gradually increased; when the growth density (OD620) of the bacteria is 0.540,...

Embodiment 2

[0039] The method for producing long-chain dibasic acid by biological fermentation of the present embodiment comprises the following steps:

[0040] (1) First-class seed tank culture: alkaline water boiling tank: pH≈12, 120℃, 4h; empty elimination: 130℃, 1h; actual elimination: adjust pH properly before elimination, so that the pH before seeding is in the range of 6.7~7.0 . The temperature is 121-123°C, the temperature is kept for 30 minutes, and the volume after the actual elimination is 1.4-1.6m 3 After the actual elimination, the temperature was rapidly lowered, and when the temperature dropped to 30 °C ± 0.5 °C, Candida tropicalis was used for inoculation and culture.

[0041] In the first-level culture process, normal culture in the early stage, when the dissolved oxygen is close to 40%, increase the air volume to ensure that the dissolved oxygen is maintained at 40%-50%, and the air volume is gradually increased; when the bacterial growth density (OD620) is 0.817, the b...

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Abstract

The invention provides a method for producing long-chain dicarboxylic acid through biological fermentation, which adopts candida tropicalis or candida vesii as a fermentation microorganism, and comprises the following steps: 1) culturing the microorganism by adopting a two-stage seeding tank, the thallus growth density (OD620) after the culture of the first-stage seeding tank is greater than 0.5, and the thallus growth density (OD620) after the culture of the second-stage seeding tank is greater than 0.5; and (2) inoculating the bacterial liquid into a fermentation tank for fermentation after the culture in the secondary seed tank is finished, and controlling the pH value within the range of 6.8-7.1 during the fermentation period. The novel method disclosed by the invention is short in fermentation period, large in alkane addition amount, high in acid yield and high in conversion rate, and meets the production requirements of energy conservation, emission reduction and cost reduction.

Description

technical field [0001] The invention relates to a method for producing long-chain dibasic acid by biological fermentation. Background technique [0002] Long chain dicarboxy acids refer to aliphatic dibasic acids (DCn) containing 9 or more carbon atoms in the carbon chain, including saturated and unsaturated dibasic acids. Fine chemical products for industrial use are important raw materials for synthesizing high-grade fragrances, high-performance engineering plastics, high-temperature dielectrics, high-grade hot melt adhesives, cold-resistant plasticizers, high-grade lubricants, high-grade paints and coatings in the chemical industry. [0003] In general, long-chain dibasic acids can be produced by chemical synthesis or biological fermentation. The chemical synthesis method has a long synthetic route, strict reaction conditions, needs to be carried out under high temperature and high pressure conditions, and has strict requirements on catalysts. Therefore, on an industrial...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12N1/16C12R1/74C12R1/72
CPCC12P7/6409C12N1/16
Inventor 王邵冰修德恒郭启健
Owner 青岛智库生物技术有限公司
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