Beauveria bassiana preparation used for preventing and controlling pine moth, its preparation method and use
A technology of Beauveria bassiana and pine caterpillars is applied in the fields of botanical equipment and methods, chemicals for biological control, applications, etc., and can solve the problems of inability to germinate spores, spores dry and die due to dehydration, and freeze to death at low temperature. To achieve the effect of promoting the occurrence of epidemics, the concentration of applied bacteria, and the rapid spread of bacteria
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Embodiment 1
[0026] Embodiment 1 Preparation of nutritional wet fungus powder Beauveria bassiana preparation of the present invention
[0027] (1) Cultivation of first-level slant strains:
[0028] 1. Preparation of slant medium:
[0029] Slant medium components and their proportions: 200g of potatoes, 20g of glucose, 20g of agar, 20g of peptone, 30g of dried insect corpses, and 1000mL of water. Its preparation steps:
[0030] ①. Weigh 30g of the dry corpse of 3-5 instar Chinese pine caterpillar larvae, add 1000mL of clear water and soak for 2 hours.
[0031] ②. Peel the potatoes and dig out the buds, weigh 200g, cut into small pieces with a length and width of about 1cm, add them to the soaked dry insect corpse liquid and boil them together, until the potatoes are soft, filter them with gauze, and take the filtrate.
[0032] ③. Add water to the filtrate to 1000mL, add 20g of peptone, and heat until completely dissolved.
[0033] ④. During the boiling process, add 20g of agar, add 20g ...
Embodiment 2
[0057] The forest application test of embodiment 2 nutrient type wet fungus powder Beauveria bassiana preparation:
[0058] (1) Application of nutrient-type wet fungus powder Beauveria bassiana preparation in northern winter: In November 2003, it was applied in the 35-year-old artificial Pinus tabulaeformis forest of Xishan Mineral Bureau Forest Farm, Lingqiu County, northern Shanxi Province, and the control area was 15 hectares. Bring the prepared nutrient-type wet fungus powder to the forest where the pine caterpillar occurs and apply it. Gently dig open the litter hidden by the pine caterpillar at the base of the tree root, spread the fungal powder evenly, and then cover the litter. The dosage is 250g / plant.
[0059] Effect inspection: In late April, before the pine caterpillars sting on the tree, the control effect is checked by sampling method. Count the percentages of healthy living insects and infected dead, and then calculate the corrected death based on the control,...
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