High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof

A Bacillus coagulans, high-density fermentation technology, applied in the field of microorganisms, can solve the problems of high bacterial volume loss rate, low liquid fermentation level, and low bacterial volume of the final product, and achieve the effect of enhancing competitiveness and reducing energy consumption

Active Publication Date: 2012-06-20
HUNAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Analyzing the reason, it is mainly because the liquid fermentation level of Bacillus coagulans is low in the industrialization process, and after the fermentation liquid is subjected to post-treatment processes such as adsorption, drying and crushing, the loss rate of the bacterial volume is relatively high, resulting in a low bacterial volume of the final product. It directly affects the use effect of Bacillus coagulans inoculum. Therefore, developing a production method of high-density Bacillus coagulans inoculum will bring infinite vitality to my country's livestock and poultry breeding industry and green ecological agriculture.

Method used

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  • High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof
  • High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof
  • High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Liquid fermentation

[0026] a) Slant seed activation: inoculate the original seed to test tube normal nutrient agar slant medium and cultivate overnight at 37°C for 24h. Ordinary nutrient agar slant medium (10g peptone, 3g beef extract, 5g sodium chloride, 20g agar, water to 1L, pH=6.5-7.2);

[0027] b) Shake flask seed culture medium: inoculate slant seeds from one loop to three loops to common nutrient broth medium, 37℃, 200r.min -1 Cultivate overnight for 24 hours; ordinary nutrient broth medium (peptone 10g, beef extract 3g, sodium chloride 5g, water to 1L, pH=6.5-7.2);

[0028] c) Inoculate the above seed liquid into a 20L fermentor for liquid culture according to 4% of the volume of the culture medium, and the volume of the culture medium is 50% of the volume of the fermentor;

[0029] Fermentation medium preparation: starch 10.6g.L -1 , Fish meal 20.2g.L -1 , Dipotassium hydrogen phosphate 2.72g.L -1 , Manganese sulfate monohydrate 0.169g.L -1 , Magnesium sulfate he...

Embodiment 2

[0044] (1) Liquid fermentation

[0045] a) Slant seed activation: inoculate the original seed to test tube normal nutrient agar slant medium and cultivate overnight at 37°C for 24h. Ordinary nutrient agar slant medium (10g peptone, 3g beef extract, 5g sodium chloride, 20g agar, add water to 1L, pH=6.5-7.2);

[0046] b) Shake flask seed culture medium: inoculate slant seeds from one loop to three loops to common nutrient broth medium, 37℃, 200r.min -1 Cultivate overnight for 24h; ordinary nutrient broth medium (peptone 10g, beef extract 3g, sodium chloride 5g, add water to 1L, pH=6.5-7.2);

[0047] c) Inoculate the above seed solution into a 20L fermentor for liquid culture according to 3% of the volume of the culture medium, and the volume of the culture medium is 40% of the volume of the fermentor;

[0048] Fermentation medium preparation: starch 9.0g.L -1 , Fish meal (conventional purchase crushed to 60 mesh) 18.0g.L -1 , Dipotassium hydrogen phosphate 2.0g.L -1 , Manganese sulfate ...

Embodiment 3

[0057] (1) Liquid fermentation

[0058] a) Slant seed activation: inoculate the original seed to test tube normal nutrient agar slant medium and cultivate overnight at 37°C for 24h. Ordinary nutrient agar slant medium (10g peptone, 3g beef extract, 5g sodium chloride, 20g agar, add water to 1L, pH=6.5-7.2);

[0059] b) Shake flask seed culture medium: inoculate slant seeds from one loop to three loops to common nutrient broth medium, 37℃, 200r.min -1 Cultivate overnight for 24 hours; ordinary nutrient broth medium (peptone 10g, beef extract 3g, sodium chloride 5g, add water to 1L, pH=6.5-7.2);

[0060] c) Inoculate the above seed liquid according to 5% of the volume of the culture medium into a 20L fermentor for liquid culture, the volume of the culture medium is 60% of the volume of the fermentor; the preparation of the fermentation medium: starch 12.0g.L -1 , Fish meal (conventional purchase crushed to 60 mesh) 22.0g.L -1 , Dipotassium hydrogen phosphate 3.2g.L -1 , Manganese sulfa...

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Abstract

The invention discloses a high-density fermenting method of bacillus coagulans for livestock and poultry, as well as a preparation prepared by the method and application thereof. The strain collection number of the bacillus coagulans is CICC (China Center of Industrial Culture Collection) No.20138. The method comprises the following steps: performing liquid fermentation on the bacillus coagulans;performing solid alternating temperature continuous fermentation; ventilating in later phase of solid fermentation; continuing fermenting and drying, and preparing a bacillus coagulans preparation initial product after the drying is completed; further crushing and screening to obtain a high-density bacillus coagulans preparation final product, wherein the number of strains of the final product is115.5*10<8>cfu.g<-1>, and the spore rate is 77.2%. The product has the effects of increasing the weight and preventing diarrhea of piglet, and the application adding concentration is 0.05wt%. The number of strain preparation is far higher than that of similar strain preparations (20*10<8>cfu.g<-1>) in the market, so that the preparation has wide market competitively in the livestock and poultry breeding industry.

Description

Technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for continuous fermentation of Bacillus coagulans liquid-solid staged temperature-changing fermentation for livestock and poultry, as well as a prepared bacterial agent and application. Background technique [0002] In my country, antibiotics have been used as feed additives for decades. They have promoted the full use of animal production performance, prevented pathogen infections, and made significant contributions to the development of intensive animal husbandry. However, recent studies have shown that the widespread use of antibiotics has also brought insurmountable disadvantages to humans, such as endogenous infections and double infections, the generation of drug resistance, the destruction of normal intestinal flora, livestock and poultry products, and Problems such as drug residues in the environment. [0003] As a new type of antibiotic substitute, microecologi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/07
Inventor 高书锋刘惠知胡新旭周映华吴胜莲缪东周小玲
Owner HUNAN INST OF MICROBIOLOGY
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