High-density fermenting method of bacillus coagulans for livestock and poultry, as well as preparation prepared by method and application thereof
A Bacillus coagulans, high-density fermentation technology, applied in the field of microorganisms, can solve the problems of high bacterial volume loss rate, low liquid fermentation level, and low bacterial volume of the final product, and achieve the effect of enhancing competitiveness and reducing energy consumption
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Embodiment 1
[0025] (1) Liquid fermentation
[0026] a) Slant seed activation: inoculate the original seed to test tube normal nutrient agar slant medium and cultivate overnight at 37°C for 24h. Ordinary nutrient agar slant medium (10g peptone, 3g beef extract, 5g sodium chloride, 20g agar, water to 1L, pH=6.5-7.2);
[0027] b) Shake flask seed culture medium: inoculate slant seeds from one loop to three loops to common nutrient broth medium, 37℃, 200r.min -1 Cultivate overnight for 24 hours; ordinary nutrient broth medium (peptone 10g, beef extract 3g, sodium chloride 5g, water to 1L, pH=6.5-7.2);
[0028] c) Inoculate the above seed liquid into a 20L fermentor for liquid culture according to 4% of the volume of the culture medium, and the volume of the culture medium is 50% of the volume of the fermentor;
[0029] Fermentation medium preparation: starch 10.6g.L -1 , Fish meal 20.2g.L -1 , Dipotassium hydrogen phosphate 2.72g.L -1 , Manganese sulfate monohydrate 0.169g.L -1 , Magnesium sulfate he...
Embodiment 2
[0044] (1) Liquid fermentation
[0045] a) Slant seed activation: inoculate the original seed to test tube normal nutrient agar slant medium and cultivate overnight at 37°C for 24h. Ordinary nutrient agar slant medium (10g peptone, 3g beef extract, 5g sodium chloride, 20g agar, add water to 1L, pH=6.5-7.2);
[0046] b) Shake flask seed culture medium: inoculate slant seeds from one loop to three loops to common nutrient broth medium, 37℃, 200r.min -1 Cultivate overnight for 24h; ordinary nutrient broth medium (peptone 10g, beef extract 3g, sodium chloride 5g, add water to 1L, pH=6.5-7.2);
[0047] c) Inoculate the above seed solution into a 20L fermentor for liquid culture according to 3% of the volume of the culture medium, and the volume of the culture medium is 40% of the volume of the fermentor;
[0048] Fermentation medium preparation: starch 9.0g.L -1 , Fish meal (conventional purchase crushed to 60 mesh) 18.0g.L -1 , Dipotassium hydrogen phosphate 2.0g.L -1 , Manganese sulfate ...
Embodiment 3
[0057] (1) Liquid fermentation
[0058] a) Slant seed activation: inoculate the original seed to test tube normal nutrient agar slant medium and cultivate overnight at 37°C for 24h. Ordinary nutrient agar slant medium (10g peptone, 3g beef extract, 5g sodium chloride, 20g agar, add water to 1L, pH=6.5-7.2);
[0059] b) Shake flask seed culture medium: inoculate slant seeds from one loop to three loops to common nutrient broth medium, 37℃, 200r.min -1 Cultivate overnight for 24 hours; ordinary nutrient broth medium (peptone 10g, beef extract 3g, sodium chloride 5g, add water to 1L, pH=6.5-7.2);
[0060] c) Inoculate the above seed liquid according to 5% of the volume of the culture medium into a 20L fermentor for liquid culture, the volume of the culture medium is 60% of the volume of the fermentor; the preparation of the fermentation medium: starch 12.0g.L -1 , Fish meal (conventional purchase crushed to 60 mesh) 22.0g.L -1 , Dipotassium hydrogen phosphate 3.2g.L -1 , Manganese sulfa...
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