95 results about "Nutrient agar" patented technology

The invention relates to Sphingomonas sp. TP-5 and a method and an application of the same for producing welan gum. A strain of the invention is prepared by separating and acclimating sugar-contained wastewater in a molasses plant, has a systematic name of Sphingomonas sp. and a preservation number of CGMCCNo.3097, not only can grow in nutrient media of beef broth, LB and nutrient agar, but also can grow in a sugar-contained inorganic-salt culture medium. The TP-5 strain can be fermented in the sterile culture medium containing sugar, inorganic salt and water at a temperature condition of 28-37 DEG C; ymotic fluid is extracted to obtain the welan gum achieving a temperature-resistant limit value of 150 DEG C; and potassium chloride in different concentrations is added so that a welan gum solution remarkably improves the viscosity, enhances the temperature stability, does not vary the viscosity with temperature and presents stronger temperature tolerance, thereby being applied to seawater drilling mud and a high salinity reservoir viscous water flooding system.

The invention provides a microorganism compound bactericide for controlling cucumber fusarium wilt and a preparation method thereof. The microorganism compound bactericide for controlling cucumber fusarium wilt is characterized by consisting of 10 to 15 parts by weight of rhodopseudanonas palustris, 30 to 40 parts by weight of bacillus subtilis, 15 to 18 parts by weight of streptomyces, 20 to 23 parts by weight of lactobacillu plantarum and 10 to 15 parts by weight of saccharomyces cerevisiae. The preparation method is characterized by comprising the following steps of: screening the rhodopseudanonas palustris, the bacillus subtilis and the streptomyces, and respectively inoculating and culturing in a flask in which nutrient agar culture media are put; mixing obtained strains with the lactobacillu plantarum and the saccharomyces cerevisiae; and inoculating mixed lawn in a liquid culture dish, and fermenting in a fermentation tank to obtain the microorganism compound bactericide. In the invention, soil ecological environment is repaired and improved under the condition of inhibiting the reproduction of fusarium wilt pathogenic bacteria and controlling pathogenetic scale and degree,meanwhile, cucumber growth and yield are improved.

The invented Rhodococcus erythropolis LSSE8-1 is preserved in "general Microbe Centre, Chinese Microbe Culture Preservation & Management Committee" in 9,10,2001, its preservation number is CGMCCNO.0643; its Gram reaction is positive, partial acidoresistant and aerobic; after 14 hr. of sheet-inserting culture at 30 deg.C on the glucose asparagine agar and nutrient agar, its hypha is started breaking from centre of colony, after 24 hr. the globular or rhabdoid corpuscle can be produced, after 72 hr. the circumference of colony can be continuously grown, there is not aerial mycelium, occasionally it has ramification, it has no requirement for vitamin B1, it contains meso diaminopimelic acid, and has characteristic arabinose and galactose, its cell wall belongs to IV type.

The invention relates to a microbial agent for preventing and treating pinellia ternate root rot as well as a preparation method and application thereof, in particular to bacillus velezensis MES861. According to the bacillus velezensis MES861, the preservation date is December 03, 2018, the name and the abbreviation of the preservation organization are China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.16857. According to the pinellia ternate root rot prevention and treatment microbial agent, fermentation liquor prepared through fermentation of the bacillus velezensis serves as the microbial agent for preventing and treating the pinellia ternate root rot. The preparation method for the microbial agent for preventing and treating the pinellia ternate root rot comprises the step that a liquid nutrient agar culture medium is inoculated with the bacillus velezensis, and shake culture is carried out so as to obtain the bacillus velezensis fermentation liquor. The bacillus velezensis has a strong inhibition effect on pathogenic bacteria such as fusarium graminearum, fusarium solani, fusarium oxysporum and F.moniliforlle, so that the occurrence of the pinellia ternate root rot is effectively prevented, and the stable yield and the income increase of pinellia ternate are promoted.

The invention provides a fungus strain A. flavus G10 for degrading polyurethane plastic. The microorganism preservation number of the fungus strain A. flavus G10 is GDMCC 60537. The invention also provides a method for culturing the fungus strain A. flavus G10. The method includes the steps of isolating the fungus strain from the intestinal tract of a cricket; adopting PU as a unique carbon sourcefor culturing the fungus strain in a liquid culture medium to obtain a culture solution; diluting the culture solution and coating a solidified nutrient agar culture medium containing tetracycline antibiotics and a potato glucose agar culture medium with the culture solution to obtain cultured growing products. The cultured growing products are subcultured on fresh plates at 30 DEG C until a single fungus strain is obtained on each plate. The fungus strain A. flavus G10 can degrade the polyurethane plastic quickly.

A method of quickly identifying bacterial wilt resistance in tobacco varieties in seedling stage comprises the main steps of A, raising seedlings of a tobacco variety under test in nutrient pots according to a floating seedling raising method; B, subjecting tobacco plants infected by bacterial wilt and collected from a field to isolation purification culture for 3-5 days via a TTC (triphenyl tetrazolium chloride) medium to obtain purified Ralstonia solanacearum; inoculating the Ralstonia solanacearum subjected to purification culture to NA (nutrient agar) medium, and culturing for 12 h to obtain an inoculation bacterial liquid; C, sucking the inoculation bacterial liquid with an injector, selecting tobacco seedlings growing with 3-4 true leaves in the nutrient pots in step A, and injecting 2-4 mu L of the inoculation bacterial liquid obliquely into a stalk of each selected tobacco seedling from its axil; D, culturing the inoculated tobacco seedlings in a sunlight culture room for 10 days, observing and recording disease attack conditions of the tobacco seedlings on 10th day of culture, calculating a disease index, and judging the bacterial wilt resistance of the tobacco variety under test. The method is simple to perform, high in measuring speed, good in results accuracy and good in result uniformity.

The invention relates to the technical field of agricultural technology and biotechnology cross technologies, and particularly discloses a method for rapidly screening low-accumulation heavy metal crop varieties and a device used by the method. The method comprises the steps that the device for rapidly screening the low-accumulation heavy metal crop varieties is manufactured, then crop seeds are subjected to vernalization, the seeds are cultivated by using a nutrient solution after the seeds are sowed in nutrient agar medium of the device, after crops are cultivated to the required period, a heavy metal solution is added to the nutrient solution, the crops are harvested after cultivation, and low-accumulation heavy metal crop variety screening is carried out. The device is made of light-proof PVC boards, so that harmful substances are not prone to be leached out to affect a research result, and a dark condition like that roots of the crops are in soil can be simulated; a centrifuge tube and small holes are simultaneously used for marking, and thus the marking is convenient; and by adopting a water culture mode for cultivation, the nutrient solution can provide nutrients and water, so that the survival rate is high, and clean and complete crop plants, particular the roots, can be obtained rapidly and accurately.

The invention relates to a method for preparing microbe bacteria liquid for treating black-odor riverway. The method comprises the following steps: collecting mud at bottom of the riverway, performing microflora diversity analysis on the mud through a high-flux sequencing experiment, performing primary screening, taking mud at the bottom of the riverway, inoculating the mud into a aseptic nitrogen-removal bacterium enrichment nutrient solution, inoculating the mud into fresh enrichment nutrient solution, detecting the ammonia nitrogen and nitrate nitrogen content in the medium, taking mixing bacteria liquid with less accumulation of nitrogen as the primarily screened flora having nitrogen removal effect; optimizing enrichment screening, inoculating the fresh optimized enrichment medium into the primarily screened nitrogen-removal bacteria liquid, adding a trace element solution in the mixing bacteria liquid, inoculating the mixing bacteria liquid added with the trace element into the liquid medium which takes calcium carbonate and sodium citrate as carbon sources, uniformly preparing an uniform breeding diluents by the mixing bacteria liquid, inoculating the material to beef extract peptone nutrient agar, uniformly distributing the materials in a flat, and culturing the material.

A growth plate or Petri plate and a method for detection and identification of microorganisms by specific or non-specific staining of colonies or microcolonies is described. Analytical substances (chromogenic or fluorogenic substrates, biochemical dyes, dyes indicators of pH, antibiotics and other biological active substances) are applied to the lower surface of a nutrient agar layer. The substances diffuse in the agar, reach cells and react with them. Because colonies or microcolonies are not removed from the media, they retain their shape, color, size and other useful characteristics for analytical purposes.

The invention provides a method for detecting the number of viable bacilli, which is characterized by comprising the following steps: weighing a bacillus sample according to a conventional method; adding the bacillus sample to a triangular flask or a test tube holding a nutrient broth germinant, wherein the broth germinant contains manganous sulfate, magnesium sulfate and ferrous sulfate; puttingthe triangular flask or the test tube in a constant-temperature water bath the temperature of which is 80 DEG C to carry out high-temperature treatment for 10-15 min; cooling with constant-temperaturerunning water; vibrating on a swing bed for 30 min, and then obtaining a bacillus culture solution; diluting the bacillus culture solution for 10 times with 0.5-1wt% of sodium chloride diluent; inoculating in a nutrient agar culture medium which is melted in advance and cooled to 50 DEG C, and after solidification, putting in a constant temperature cabinet the temperature of which is 37 DEG C tocarry out inverted culture for 48 hours, and then seeing that visible colonies grow out on a flat plate; and calculating according to the conventional method to obtain the number of the viable bacilliin the sample. The invention has the advantage of high accuracy.

The invention discloses a rapid separation method of antagonistic bacteria against pathogens of konjac soft rot disease, which comprises the following steps: A) taking konjac tissues infected by the soft rot disease; B) inoculating the tissues at the interface of diseased and healthy tissues onto a nutrient agar culture medium after carrying out surface disinfection; C) streaking the bacteria grown out on the sides of the tissues, and selecting a single colony for purification and preservation; D) adopting a distinctive Erwinia crystal violet polypectin selective culture medium (CVP) to screen the pathogens of the konjac soft rot disease; E) adopting the point connection method to carry out primary screening of antagonistic bacteria; and F) adopting the oxford cup method to carry out secondary screening of the antagonistic bacteria. The method has simple operation and easy operation, and can rapidly and effectively screen out the antagonistic bacteria against the pathogens of the konjac soft rot disease and simultaneously provide a concept for rapid screening of the antagonistic bacteria against other crop diseases.

A preparation method of a halophilic decontamination bacterial agent and the bacterial agent prepared by the same belong to the technical field of biological treatment of salinity wastewater. The preparation method comprises the following steps: 1) inoculating the test tube strain of the halophilic decontamination bacterium into nutrient agar for culturing; 2) inoculating the cultured strain into a fermentor medium of preset volumetric quantity according to the inoculation quantity which is 1-2% of the medium weight to be cultured till logarithmic growth phase; and 3) in the fermentor production process, maintaining the ventilation volume of aseptic air to be 4.0-6.0L / min, the stirring rate to be 60-120r / min, the culture temperature to be 25-40 DEG C and the full-process culture time to be 24-48h, subpackaging the culture solution after fermentation or adopting aseptic attachments for adsorption and drying, thus preparing the solid bacterial agent. The strain of the bacterial agent was collected in China General Microbiological Culture Collection Center (CGMCC), with collection number of CGMCC No.3314. The invention adopts the bioaugmentation technology, greatly improves the capability of treating salinity wastewater and has low cost and significant effects.

The invention discloses a substrate type seedling tray for cultivating strong mechanical transplanting rice seedlings. The seedling tray includes, by weight, 11-13 parts of mushroom dregs, 5-7 parts of straws, 0.5-1.5 parts of degradable paper fibers, 0.5-1.5 parts of nutrient agar and 6-7 parts of water. Through the seedling tray, seedling growing costs can be effectively reduced, agriculture wastes can be changed into treasures, and cyclic utilization and environment protection can be realized; and through the integral treatment of substances such as seedling growing trays, substrates and nutrients, strong seedlings can be cultivated, labor can be saved, and therefore, technical support and guarantees can be provided for large-area rice production.

This invention describes method for rapid detection and identification of colonies and micro-colonies of microorganisms growing on the surface of certain nutrient agar under certain porous material(s) impregnated by chromogenic and / or flurogenic substrates and supportive substances. Growth in the space between nutrient agar and impregnated porous material allows limiting the negative influence of unfavorable substrates and substances and allows to reveal color or fluorescence spots at early stage of micro-colony growth.

The invention discloses a method for detecting the number of live spore in Bacillus subtilis powder of 5 hundred billion live spores / gram, comprising the steps of: A, formulation of reagent and culture medium as well as preparation of plating medium; B, pretreatment of sample; C, dilution of sample; D, sample coating and culture: 0.1mL of each of the samples which are diluted to 10 is absorbed to 5 nutrient agar plates on each of which sample numbers, dates and dilutions are marked, a coating rod is used to coat the samples that are put in the plates, until all the samples are absorbed by the culture medium, after three plates are coated, the coating rod is used to coat back and forth on the blank nutrient agar plates 20 times; another coating rod is used to coat the samples that are in the rest two plates until all the samples are absorbed by the culture medium, afterwards, the coating rod is used to coat back and forth on the blank nutrient agar plates 20 times, after the coating is completed, the culture medium is put into a 37 DEG C constant-temperature incubator for 16hr-20hr; E, counting. The method is convenient and simple in operation, high in sensitivity and suitable for the detection of a large number of samples, counting the number of single colony on the plate and calculating the number of pore of Bacillus subtilis in the sample.

The invention relates to a method for rapidly detecting antibacterial properties of materials and belongs to the technical field of material detection. The method comprises the following steps: takinga nutrient agar medium slant fresh culture of a test strain, repeatedly washing the slope by using a PBS (Phosphate Buffered Solution), washing lawn, oscillating and uniformly mixing, diluting and storing in a refrigerator at the temperature of 4 DEG C for later use; wiping surfaces of a control sample material and a detected sample material by using a 70% ethanol solution, washing by using sterile water and naturally drying; putting the control sample material and the detected sample material into a triangular flask, adding 95mL of PBS, uniformly mixing, adding 5mL of a pre-made bacterial suspension and carrying out oscillating contact treatment; and adding a 2,3,5-triphenyltetramlium chloride (TTC) standard solution into the bacteria solution after the oscillating contact treatment, oscillating in the dark, centrifuging 10mL of the bacteria solution, adding 10mL of acetone into the precipitate to carry out extraction, centrifuging the extract, determining OD485 through the supernatant and calculating the antibacterial rate according to a TTC standard curve. The method disclosed by the invention has a broad application prospect when serving as a screening method for qualitativelydetecting antibacterial activity of the materials.

The invention relates to a culture method for COD (Chemical Oxygen Demand) degrading bacteria, which includes the following steps: (1) a nutrient agar culture medium is prepared; (2) COD degrading bacteria are activated in the nutrient broth culture medium; (3) a pipette is used for respectively extracting 200mL of diluted COD degrading bacteria solution. The culture method has the advantages that the COD degrading bacteria cultured by the culture method can effectively increase the COD degradation rate, and cannot pollute the environment.

The invention belongs to the technical field of microbial agents, and discloses a bacillus megaterium strain, and a preparation method and application thereof. The collection number of the bacillus megaterium strain is CCTCC (China Center for Type Culture Collection) M2018430. The preparation method for the bacillus megaterium strain comprises the following steps of: culture activation: carrying out activated culture on the bacillus megaterium preserved at a low temperature on an NA (Nutrient Agar) solid culture medium to obtain the activation seed of the bacillus megaterium; and culture seedsolution preparation: inoculating the bacillus megaterium obtained after activation is conducted into an NA liquid culture medium, and carrying out rejuvenation culture. The bacillus megaterium disclosed by the invention has the functions of stimulating crops to grow, regulating soil acility and alkalinity, accelerating soils to form a granular structure, inhibiting the growth of soil pathogenic bacteria and improving the micro-ecological environment of soils. By the application of the Bacillus megaterium strain YM11, the micro-ecological environment of a maize rhizosphere can be improved, rhizosphere nutrient element compositions are improved, the micro-ecological environment of the maize rhizosphere is changed, soil microbial diversity is increased, soil physicochemical properties are improved, and the yield of maize on a saline-alkali soil is increased.

The invention provides cellar-stored raw wine and a production method thereof, and relates to the technical field of storage of raw wine. The production method of the cellar-stored raw wine comprisesthe following steps: collecting air microorganisms in a koji pharmacy with a nutrient agar plate, and enabling bacteria-bearing particles suspending in the air to impact on the surface of the nutrientagar plate; culturing the bacteria-bearing nutrient agar plate, then drying and crushing to obtain spore powder; spreading the spore powder on the outer surfaces of a plurality of wine jars, made byfiring the soil, in a wine cellar; storing the wine jars for 1-5 years until the spore powder forms wine moss on the surfaces of the wine jars, and enabling the wine moss and the base liquor to be subjected to exchange of materials so as to form the cellar-stored base liquor. According to the production method of the cellar-stored raw wine, the air microorganisms are collected in the koji pharmacyand are then cultured, dried and crushed so as to form the spore powder, the spore powder is enabled to form the wine moss on the surfaces of the wine jars, and the wine moss and the raw wine in thewine jars are enabled to be subjected to exchange of materials, so that the quality and uniformity of the raw wine are better improved, and the formed cellar-stored raw wine is better in quality.

The invention discloses a long-term preservation and activation method of an efficient degumming strain of ramie. The long-term preservation and activation method comprises the following steps: picking a purified classical single colony lawn, scribing in a test tube solid nutrient agar culture-medium, and culturing in a constant temperature incubator with the temperature of 35DEG C for 20 hours; sheathing by a large test tube, plugging a rubber hollow glass tube, and coating a junction of the large test tube and rubber with paraffin; after a vacuum pump vaccumizes, sintering the hollow glass tube by using an alcohol blast burner, sealing and storing. After the efficient degumming strain of the ramie stored for 10 years by adopting the method is subjected to activation treatment, the test live bacteria amount can reach 1.02*109cfu / ml or above; after activated state bacteria liquid is inoculated to the ramie, and is soaked and fermented for 10.5 hours, ramie fibers are completely dispersed, the mass is reduced by 27 percent or above, and efficient degumming of the ramie is realized. According to the long-term preservation and activation method disclosed by the invention, the stored strain is small in required space and is long in storage time; the survival period of the strain exceeds 10 years; by one-time inoculation, transformed generation is not needed over 10 years; the strain is easy to activate and stable in performance.

The invention discloses a method for detecting antibacterial property of a silkworm excrement product, comprising the following steps: 1, scribing on a slant of a nutrient agar medium for inoculating to obtain slant preserved bacteria; 2, picking one typical bacterial colony from an agar slant, and inoculating to nutrient meat soup to obtain a bacterial suspension; 3, drawing and transferring the bacterial suspension into a test tube holding PBS, mixing well, and diluting to obtain inoculation bacterial suspension; 4, making sampling bags; 5, adding sterile water to one sampling bag for pre-soaking to obtain a blank control, adding an antibacterial silkworm excrement product and a non-antibacterial silkworm excrement product respectively into other sampling bags, and adding sterile water for pre-soaking to obtain a sample to be detected and a control sample; 6, placing the sample to be detected, the control sample and the blank control respectively on an agar plate surface coated with the inoculation bacterial suspension, taking a small amount of the inoculation bacterial suspension, and dropwise adding to the three samples; 7, during culturing, recording and acquiring antibacterial property of the silkworm excrement product. In the above manner, the method is simple to perform, high in speed and low in usage cost.

The invention discloses a method for preparing bacteria liquid for Riemerella anatipestifer vaccine, which comprises the propagation and identification of the first-level seed, and the propagation and fermentation of the second-level seed; the propagation and identification of the first-level seeds comprises the following steps of: inoculating freeze-dry basic microbial strain into the nutrient broth containing 10 percent of fetal bovine serum, and culturing the nutrient broth for 24h at the temperature of 37 DEG C; inoculating the obtained product onto the nutrient agar plate containing 10 percent of fetal bovine serum by streaking, then culturing the obtained product in the environment containing 5 to 10 percent of CO2 or candle jar for 24h to 48h at the temperature of 37 DEG C; after that, selecting several typical colonies, inoculating the typical colonies onto the nutrient agar slant containing 10 percent of fetal bovine serum, culturing the product in the environment containing 5 to 10 percent of CO2 or candle jar for 24h at the temperature of 37 DEG C, and obtaining first-level seeds; the propagation of the second-level seeds comprises the following steps of: inoculating the first-level seeds into the nutrient broth containing 10 percent of fetal bovine serum; and culturing the nutrient broth for 24h at the temperature of 37 DEG C; sampling the obtained product for pureness test; and if obtained product is tested to be qualified, keeping the product at the temperature of 2 to 8 DEG C, wherein the service life thereof is not more than five days, the culture medium used in the fermentation is the N-J synthetic medium containing one percent of lysed whole blood, the fermenting microbial strain is inoculated in an amount which is 2 percent based on that of the second-level seed liquid, the fermentation manner is liquid fermentation and culture in a culture tank, and the culture condition is continuously culturing 24h at the temperature of 37 DEG C.

The invention discloses a bacteria culture medium and a preparation method thereof. A nutrient agar medium is taken as a basic culture medium, wherein a water-retaining agent and a coloring agent are added in the basic culture medium, the weight ratio of the nutrient agar, the water, the water-retaining agent to the coloring agent is (37-39):1000:(0.2-4): (0.02-0.09); and during the preparation process, each component is weighed, mixed and sterilized. By adopting the water-retaining agent in the obtained bacteria culture medium, the moisture of the culture medium can be kept and the accuracy of the measuring result influenced by the water loss and dryness can be avoided; by adopting the coloring agent, most bacterial colonies are dyed red or pink so that the detecting result can be conveniently observed; and simultaneously the preparation method is reasonable in design and simple and convenient in operation.

The invention belongs to the field of microorganisms, and relates to a method for screening sodium chloride-resistant bacillus licheniformis, which comprises the following steps: (1) screening: through taking the nutrient agar as an agar medium, the slant culture of a test tube is firstly carried out on the bacillus licheniformis which has the number of 1.0518 and is preserved in the China general microbiological culture collection center (CGMCC), then after the slant streak cultivation of a culture vessel is carried out, the single bacterial colony of the typical bacillus licheniformis is screened; the typical bacillus licheniformis is transferred to the inclined plane of the nutrient agar test tube to culture; and (2) domestication: the sodium chloride is added in the nutrient agar culture medium to prepare the culture vessels with different sodium chloride concentrations, the sodium chloride concentration is gradually improved for screening and domestication culture, and the bacillus licheniformis which can resist the sodium chloride with higher concentration is screened. The bacillus licheniformis which can resist the sodium chloride with the mass concentration of 12.5% is obtained, and can be used for fertilizer.

The present invention relates to bacteria culture medium, and is especially one kind of bacteria culture medium with nutritious agar culture medium as basic culture medium and its preparation process. The basic culture medium contains nutritious agar, water, water preserving agent and dye in the weight ratio of 36-40 to 1000 to 0.1-5 to 0.01-0.1 and is prepared through mixing in the weight proportion and sterilizing. The present invention has the features of convenient observation and detection, simple preparation process and convenient operation.

The invention discloses a method for separating Hanseniaspora guilliermondii from red wine production raw materials or pit soil and purifying and identifying Hanseniaspora guilliermondii, comprising the steps of carrying out purification and separation on red wine production raw materials and pit soil, and conducting strain identification by combining WL nutrient agar medium morphology identification method and microbial taxonomy method to finally obtain a purified Hanseniaspora guilliermondii strain. According to the invention, the method disclosed herein in beneficial for using pure strain to culture red wind with unique pleasant fragrance; and the method has the characteristics of no bacterial contamination, easy preservation of the strain, rapid and simple operation, and stable product quality.

The invention belongs to the field of microbes, and relates to a screening method of bacillus subtilis with sodium chloride tolerance. The method comprises the following steps of: (1) screening, wherein bacillus subtilis with the CGMCC (China General Microbiological Culture Collection Center) preservation number of 1.0769 is subjected to test tube slant culture and a petri dish slant streak culture by using nutrient agar as a culture medium to screen a single colony of typical bacillus subtilis, and the typical bacillus subtilis is transformed to a slant of a nutrient agar test tube for culturing; and (2) acclimation, wherein sodium chloride is added into the nutrient agar culture medium to prepare petri dishes with different concentrations of sodium chloride. According to the method, thescreening and acclimating cultivation are carried out by gradually increasing the concentration of sodium chloride, so that the bacillus subtilis capable of tolerating high-concentration sodium chloride can be screened. The bacillus subtilis capable of tolerating 12.5wt% sodium chloride can be prepared and can be used in a fertilizer.

The invention discloses screening, identification and biocontrol activity determination methods for acidophilic PGPR strains. The screening method comprises the following steps of: 1, screening of theacidophilic PGPR strains: adjusting the pH value of a nutrient agar culture medium plate to 5.5 for screening of acidophilic bacteria, wherein rhizosphere soil sample suspension is not subjected to heat treatment at 80 DEG C; and 2, determination of the bacteriostatic activity of the strains under different pH conditions: (1) preparing fermentation liquor of test strains, namely inoculating activated strains into NB with different pH values in 1.1. The invention relates to the technical field of tobaccos. The screening, identification and biocontrol activity determination methods for the acidophilic PGPR strains are based on treatment of bacteria with bacteria, bacteriostatic activity, disease prevention and growth promotion effects and related biocontrol characteristics of the strains onralstonia solanacearum are studied under acidic and neutral conditions respectively, the strains are classified and identified by combining BIOLOG-GEN-III identification and 16SrDNA gene sequence homology analysis, and a foundation is laid for providing efficient microbial resources for improving the biocontrol effect of tobacco bacterial wilt in acidic soil and application of the efficient microbial resources.