12 results about "Nutrient agar" patented technology

The utility model discloses a contact type bacteria culture dish, and aims to provide a culture dish which has a larger contact area and is provided with an agar culture medium, and the key point of the technical scheme is that the culture dish comprises a dish body, a dish cover and a nutrient agar culture medium in the dish body, and is characterized in that the bottom of the dish body protrudes upwards; first net-shaped bulges for uniformly dividing the inner part of the dish body into a plurality of areas are arranged on the bottom surface of the inner side of the dish body. The culture dish is suitable for the field of microorganism devices.

The bacterial strain Arasana bacillus (CECT 30655) belongs to Gram-positive bacterial flora and bacillus subtilis, and is a stimulant capable of stimulating adaptive metabolism of plants to resist water stress, an improving agent for plant yield and nutrition and an improving agent for polyphenol content. The strain is separated from rhizosphere soil of pinus coast, is cultured on a nutrient agar culture medium (PCA), and is identified as a new strain from the aspects of morphology, biochemistry and genetics for identification through whole genome sequencing. Under a water stress condition, the strain can enhance CO2 fixation and transpiration, optimize photosynthesis energy capture and reduce oxidative stress, so that the strain can be used for increasing yield; as a biological fertilizer, nutrient absorption can be promoted no matter whether water stress exists or not; and the content of antioxidant polyphenols in plant species with medicinal or edible values can also be improved, so that the fruit quality is improved.

The invention relates to the technical field of microbial strains, and provides torulaspora delbrueckii, and the preservation number of the torulaspora delbrueckii is CGMCC (China General Microbiological Culture Collection Center) NO.7.581. The invention further discloses a screening and identifying culture medium which is used for screening, identifying and culturing the torulaspora delbrueckii. The screening and identifying culture medium comprises a nutrient agar culture medium, a sugar fermentation culture medium, an assimilation carbon source culture medium, an assimilation nitrogen source culture medium and a YPD basal culture medium. The torulaspora delbrueckii is screened from the mousse fruit wine and is used for mousse fermentation, the defect that the taste and flavor of existing saccharomycetes are similar in the fermentation process can be overcome, and the traditional flavor and taste of mousse can be reserved. And the contents of glucose, peptone and yeast extract powder in the culture medium are optimized through a single factor test and a response surface method, so that the amount of torulaspora delbrueckii is further increased, and theoretical guidance is provided for industrial application of mousalaisi brewing.

A culture medium preparation device effectively solves the problems that an existing nutrient agar culture medium is complex in preparation operation and low in efficiency. Comprising a main body box, a sliding block is arranged in the main body box, a fixed box is fixed to the front end of the main body box, a rotating box is coaxially arranged at the upper end of the fixed box, a plurality of rotating columns are rotationally connected to the rotating box, the upper ends of the rotating columns penetrate through the rotating box, eccentric boxes are eccentrically fixed to the rotating columns, movable columns are coaxially and slidably connected to the eccentric boxes, and a plurality of limiting claws are arranged on the eccentric boxes; two rotating columns are rotationally connected into the main body box; two fixed blocks are fixed at the front end of the main body box, through holes are respectively formed in the upper and lower sides of the fixed blocks, a baffle frame is slidably connected to the fixed blocks, a through hole is formed in the upper end of the baffle frame, a waist-shaped groove is formed in the lower end of the baffle frame, a quantitative bottle is detachably connected in a notch of each fixed block, and an output pipe is fixed at the lower end of each fixed block; the proportioning stability is realized by utilizing the quantitative bottles with different sizes, and objects such as an electronic scale and a measuring cylinder are not needed for weighing.

The invention discloses a layered gel culture technology constructed by using environmental extracts, and belongs to the field of environmental microorganism culture. According to the technology, an environmental sample is separated into a particle phase and a liquid phase through water extraction, the particle phase and the liquid phase are respectively prepared into a lower-layer solid-phase matrix and an upper-layer gel phase with gel (preferably agar), a vertical nutrition / signal slow-release gradient is formed in a culture dish, and particle attachment sites are provided; and inoculating the environmental microorganisms on the upper layer or the surface for culture. Compared with nutrient agar or extremely simple water agar, the method has the advantages that more bacterial colonies and higher distinguishable form number can be obtained under the repeatable condition, and the community diversity and the OTU / ASV overlapping degree with an original sample are improved; rare / slow-growing groups can be stably separated, and subsequent 16S / metagenome analysis and agar block bacteriostasis primary screening are facilitated. Meanwhile, the invention provides a corresponding culture medium composition and a kit / prefabricated layered culture dish. According to the scheme, operation is easy and convenient, parameters can be standardized, and the method is suitable for isolated culture and ecological function research of environmental samples such as soil, sediments and low-solid water samples.

The present application relates to the technical field of microbial separation, and particularly relates to a separation method and application of intestinal probiotic escherichia; the separation method of intestinal probiotic escherichia is to take bovine large intestine content as raw material, to realize pre-screening of potential colonization strains through mucus anaerobic targeted enrichment, then to obtain resistant colonies through bile salt-short chain fatty acid stress primary screening, to scrape the colonies with metallic luster and fluorescence quenching ring through FITC-labeled mucus fluorescence indication secondary screening, and finally to obtain target strains through multiple PCR screening of negative virulence gene-positive functional gene, to realize directional separation of intestinal probiotic escherichia after purification of nutrient agar; the present application realizes targeted enrichment of intestinal colonization bacteria, environmental tolerance pressure screening and mucus binding visualization identification, solves the problems of strong randomness of traditional probiotic screening and inability to predict colonization potential, and provides a new strategy of efficient and safe probiotic strain screening for development of functional food and microecological preparation.

The utility model discloses a hand bacteria collection device, and relates to the technical field of bacteria detection, the hand bacteria collection device comprises a culture dish bottom and a culture dish cover, the culture dish cover and the culture dish bottom are palm-shaped, the culture dish cover and the culture dish bottom are buckled, and the culture dish cover is hinged to one side of the culture dish bottom through a silica gel connecting piece; a palm center groove and a palm back groove are formed in the culture dish bottom and the culture dish bottom respectively and filled with nutrient agar culture media, and the culture dish bottom and the culture dish cover are in a hand shape after being closed. According to the invention, bacteria on the palm and the back of the hand can be detected at the same time, the distribution of the bacteria on the hand can be comprehensively known, especially, a positive propaganda effect on hand hygiene of children is achieved, the children are prompted to know the importance of the hand hygiene, and thus the awareness of the children on entering from the mouth is improved.

The utility model relates to the technical field of nutrient agar storage and distribution, and aims to solve the problems of inconvenience in taking, cross contamination and risk of deterioration of powder in a tank in clinical laboratory operation when the nutrient agar is stored in the tank in the prior art. The utility model provides an integrated nutrient agar storing and blending bag. The integrated nutrient agar storing and blending bag comprises a bag body and a material guiding assembly. A separation air bag is arranged in the bag body; the bag body is divided into a pure water cavity and a powder cavity through the separation air bag. The material guide assembly is communicated with the powder cavity; the material guiding assembly is arranged in the bag body in a penetrating mode. The integrated nutrient agar storing and blending bag provided by the utility model is convenient for storing the nutrient agar, is also convenient for direct use, does not need to be scooped up repeatedly or weighed manually, is convenient and practical, and solves the problems that the nutrient agar stored in a traditional tank is polluted and deteriorated.

The utility model belongs to the technical field of tool boxes, and particularly discloses a bacteria separation and identification tool box which comprises a box body, a top cover is arranged at the top of the box body, a mounting groove is formed in the bottom of the top cover, the surface of the box body is connected with the interior of the mounting groove in a sliding mode, two symmetrical rotating shafts are fixedly connected to the outer surface of the top cover, and the rotating shafts are arranged on the top cover. A handle is arranged on the outer surface of the top cover, the cross section of the handle is in a [shape, a fixing block is fixedly connected to the outer surface of the box body, and an arc surface is arranged at the bottom of the fixing block. Nutrient agar powder, a glass slide, a gram staining solution, a washing bottle, a disposable sterile plate and other tools can be placed in the box body through the two first partition plates, a worker enables a two-way threaded rod to rotate, and the two-way threaded rod can drive the two first partition plates to move relatively through two sections of opposite threads on the surface of the two-way threaded rod; and the space in the box body is adjusted.

The invention relates to a bacterial strain Peribacillus aracenensis (CECT 30655), a microorganism of the Gram-positive group of bacteria, genus Peribacillus, stimulator of plant adaptive metabolism against water stress, enhancer of plant production and nutrition, and enhancer of polyphenol content. This strain, isolated from the rhizosphere of Pinus pinaster, in nutrient agar (PCA), has been characterised from a morphological, biochemical and genetic point of view by sequencing its entire genome, being identified as a new species. It can be used to increase production under conditions of water stress, due to its ability to increase CO2 fixation and transpiration, optimising energy capture through photosynthesis and reducing oxidative stress. It can also be used as a biofertiliser, due to its ability to enhance nutrient absorption both in the presence and in the absence of water stress, as well as to enhance the quality of fruit, due to its ability to increase antioxidant polyphenols in plant species of pharmacological and food interest.

The utility model discloses a nutrition agar culture medium device, relates to microbial cultivation technical field, nutrition agar culture medium device includes incubator, vibration mechanism and a plurality of spacing rings, the incubator forms the accommodation cavity, vibration mechanism is provided in the accommodation cavity, the plurality of spacing rings are provided in the vibration mechanism, the inner side wall of spacing ring is provided with the accommodation groove, and the spacing ring is provided with the accommodation groove. The limiting ring is provided with a containing groove, the containing groove is provided with a plurality of air bags, the air bags are distributed in the circumferential direction of the limiting ring, the air bags can be inflated and deflated, the air bags can expand to protrude out of the inner side wall of the limiting ring when inflated, and the air bags can contract to be contained in the containing groove when deflated. According to the nutrient agar culture medium device, damage caused by collision between a bottle body loaded with strains and nutrient agar culture media and the limiting ring in the shaking process can be avoided, and the nutrient agar culture medium device can be suitable for bottle bodies with different diameters and is better in practicability.

A system for evaluating the plant growth-promoting rhizobacterial properties (PGPR) of endophytic bacteria isolated from Acanthus ilicifolius L., and for assessing plant growth parameters, consisting of: a) an isolation unit for isolating and purifying endophytic bacteria from leaves of Acanthus ilicifolius L., the isolation unit comprising: • Surface sterilization device configured to treat leaf explants successively with Tween20 solution, 70% ethanol and 1% mercuric chloride, • Grinding device for homogenizing leaf tissue with phosphate buffer solution, • Incubation chamber configured for the cultivation of bacterial colonies on nutrient agar at 37°C; b) a characterization unit for the morphological, biochemical and molecular characterization of isolated bacteria, wherein the characterization unit comprises the following: • Equipment for Gram staining, • Equipment for biochemical tests for performing the indole test, the methyl red test, the citrate utilization test, the H2S production test, the gelatin hydrolysis test, the starch hydrolysis test and the catalase test, and • Molecular analysis equipment configured for 16S rRNA sequencing, including DNA isolation apparatus, PCR thermocycler and DNA sequencer; c) an optimization unit configured to analyze the culture conditions for maximum bacterial growth at different temperatures and pH values, the optimization unit comprising a colorimeter configured to measure absorbance at 610 nm to generate bacterial growth curves; d) a PGPR property evaluation unit for assessing the plant growth-promoting potential of isolated bacteria, wherein the PGPR property evaluation unit comprises the following: • Testing equipment for indole-3-acetic acid (IAA) production using the Salkowski color test, • Test apparatus for phosphate solubilization using the Pikovskaya medium • Devices for testing siderophore production using chromazurol S-agar (CAS agar) and • Test apparatus for the activity of 1-amino-1-cyclopropane-1-carboxylate (ACC) deaminase using Dworkin and Foster minimal salt medium; and e) a plant growth evaluation unit configured for in vitro analysis of the effects of bacterial inoculum on plant growth parameters such as stem length, root length, leaf length and chlorophyll content.