95 results about "Nutrient agar" patented technology

The invention relates to Sphingomonas sp. TP-5 and a method and an application of the same for producing welan gum. A strain of the invention is prepared by separating and acclimating sugar-contained wastewater in a molasses plant, has a systematic name of Sphingomonas sp. and a preservation number of CGMCCNo.3097, not only can grow in nutrient media of beef broth, LB and nutrient agar, but also can grow in a sugar-contained inorganic-salt culture medium. The TP-5 strain can be fermented in the sterile culture medium containing sugar, inorganic salt and water at a temperature condition of 28-37 DEG C; ymotic fluid is extracted to obtain the welan gum achieving a temperature-resistant limit value of 150 DEG C; and potassium chloride in different concentrations is added so that a welan gum solution remarkably improves the viscosity, enhances the temperature stability, does not vary the viscosity with temperature and presents stronger temperature tolerance, thereby being applied to seawater drilling mud and a high salinity reservoir viscous water flooding system.

The invention provides a method for detecting the number of viable bacilli, which is characterized by comprising the following steps: weighing a bacillus sample according to a conventional method; adding the bacillus sample to a triangular flask or a test tube holding a nutrient broth germinant, wherein the broth germinant contains manganous sulfate, magnesium sulfate and ferrous sulfate; puttingthe triangular flask or the test tube in a constant-temperature water bath the temperature of which is 80 DEG C to carry out high-temperature treatment for 10-15 min; cooling with constant-temperaturerunning water; vibrating on a swing bed for 30 min, and then obtaining a bacillus culture solution; diluting the bacillus culture solution for 10 times with 0.5-1wt% of sodium chloride diluent; inoculating in a nutrient agar culture medium which is melted in advance and cooled to 50 DEG C, and after solidification, putting in a constant temperature cabinet the temperature of which is 37 DEG C tocarry out inverted culture for 48 hours, and then seeing that visible colonies grow out on a flat plate; and calculating according to the conventional method to obtain the number of the viable bacilliin the sample. The invention has the advantage of high accuracy.

A preparation method of a halophilic decontamination bacterial agent and the bacterial agent prepared by the same belong to the technical field of biological treatment of salinity wastewater. The preparation method comprises the following steps: 1) inoculating the test tube strain of the halophilic decontamination bacterium into nutrient agar for culturing; 2) inoculating the cultured strain into a fermentor medium of preset volumetric quantity according to the inoculation quantity which is 1-2% of the medium weight to be cultured till logarithmic growth phase; and 3) in the fermentor production process, maintaining the ventilation volume of aseptic air to be 4.0-6.0L / min, the stirring rate to be 60-120r / min, the culture temperature to be 25-40 DEG C and the full-process culture time to be 24-48h, subpackaging the culture solution after fermentation or adopting aseptic attachments for adsorption and drying, thus preparing the solid bacterial agent. The strain of the bacterial agent was collected in China General Microbiological Culture Collection Center (CGMCC), with collection number of CGMCC No.3314. The invention adopts the bioaugmentation technology, greatly improves the capability of treating salinity wastewater and has low cost and significant effects.

The invention relates to a culture method for COD (Chemical Oxygen Demand) degrading bacteria, which includes the following steps: (1) a nutrient agar culture medium is prepared; (2) COD degrading bacteria are activated in the nutrient broth culture medium; (3) a pipette is used for respectively extracting 200mL of diluted COD degrading bacteria solution. The culture method has the advantages that the COD degrading bacteria cultured by the culture method can effectively increase the COD degradation rate, and cannot pollute the environment.

The invention belongs to the technical field of microbial agents, and discloses a bacillus megaterium strain, and a preparation method and application thereof. The collection number of the bacillus megaterium strain is CCTCC (China Center for Type Culture Collection) M2018430. The preparation method for the bacillus megaterium strain comprises the following steps of: culture activation: carrying out activated culture on the bacillus megaterium preserved at a low temperature on an NA (Nutrient Agar) solid culture medium to obtain the activation seed of the bacillus megaterium; and culture seedsolution preparation: inoculating the bacillus megaterium obtained after activation is conducted into an NA liquid culture medium, and carrying out rejuvenation culture. The bacillus megaterium disclosed by the invention has the functions of stimulating crops to grow, regulating soil acility and alkalinity, accelerating soils to form a granular structure, inhibiting the growth of soil pathogenic bacteria and improving the micro-ecological environment of soils. By the application of the Bacillus megaterium strain YM11, the micro-ecological environment of a maize rhizosphere can be improved, rhizosphere nutrient element compositions are improved, the micro-ecological environment of the maize rhizosphere is changed, soil microbial diversity is increased, soil physicochemical properties are improved, and the yield of maize on a saline-alkali soil is increased.

The invention discloses a long-term preservation and activation method of an efficient degumming strain of ramie. The long-term preservation and activation method comprises the following steps: picking a purified classical single colony lawn, scribing in a test tube solid nutrient agar culture-medium, and culturing in a constant temperature incubator with the temperature of 35DEG C for 20 hours; sheathing by a large test tube, plugging a rubber hollow glass tube, and coating a junction of the large test tube and rubber with paraffin; after a vacuum pump vaccumizes, sintering the hollow glass tube by using an alcohol blast burner, sealing and storing. After the efficient degumming strain of the ramie stored for 10 years by adopting the method is subjected to activation treatment, the test live bacteria amount can reach 1.02*109cfu / ml or above; after activated state bacteria liquid is inoculated to the ramie, and is soaked and fermented for 10.5 hours, ramie fibers are completely dispersed, the mass is reduced by 27 percent or above, and efficient degumming of the ramie is realized. According to the long-term preservation and activation method disclosed by the invention, the stored strain is small in required space and is long in storage time; the survival period of the strain exceeds 10 years; by one-time inoculation, transformed generation is not needed over 10 years; the strain is easy to activate and stable in performance.

The invention discloses a method for preparing bacteria liquid for Riemerella anatipestifer vaccine, which comprises the propagation and identification of the first-level seed, and the propagation and fermentation of the second-level seed; the propagation and identification of the first-level seeds comprises the following steps of: inoculating freeze-dry basic microbial strain into the nutrient broth containing 10 percent of fetal bovine serum, and culturing the nutrient broth for 24h at the temperature of 37 DEG C; inoculating the obtained product onto the nutrient agar plate containing 10 percent of fetal bovine serum by streaking, then culturing the obtained product in the environment containing 5 to 10 percent of CO2 or candle jar for 24h to 48h at the temperature of 37 DEG C; after that, selecting several typical colonies, inoculating the typical colonies onto the nutrient agar slant containing 10 percent of fetal bovine serum, culturing the product in the environment containing 5 to 10 percent of CO2 or candle jar for 24h at the temperature of 37 DEG C, and obtaining first-level seeds; the propagation of the second-level seeds comprises the following steps of: inoculating the first-level seeds into the nutrient broth containing 10 percent of fetal bovine serum; and culturing the nutrient broth for 24h at the temperature of 37 DEG C; sampling the obtained product for pureness test; and if obtained product is tested to be qualified, keeping the product at the temperature of 2 to 8 DEG C, wherein the service life thereof is not more than five days, the culture medium used in the fermentation is the N-J synthetic medium containing one percent of lysed whole blood, the fermenting microbial strain is inoculated in an amount which is 2 percent based on that of the second-level seed liquid, the fermentation manner is liquid fermentation and culture in a culture tank, and the culture condition is continuously culturing 24h at the temperature of 37 DEG C.

The invention discloses a method for separating Hanseniaspora guilliermondii from red wine production raw materials or pit soil and purifying and identifying Hanseniaspora guilliermondii, comprising the steps of carrying out purification and separation on red wine production raw materials and pit soil, and conducting strain identification by combining WL nutrient agar medium morphology identification method and microbial taxonomy method to finally obtain a purified Hanseniaspora guilliermondii strain. According to the invention, the method disclosed herein in beneficial for using pure strain to culture red wind with unique pleasant fragrance; and the method has the characteristics of no bacterial contamination, easy preservation of the strain, rapid and simple operation, and stable product quality.

The invention provides a fungus strain A.flavus G10 for degrading polyurethane plastic. The microbial preservation number of the fungus strain A.flavus G10 is GDMCC 60537. The invention also providesa method for culturing the fungus strain A.flavus G10. The method comprises the following steps that the fungus strain is separated from the intestinal tract of a cricket; PU serves as a unique carbonsource, the fungus strain is cultured in a liquid culture medium to obtain a culture solution; the culture solution is diluted and coated on a solidified nutrient agar culture medium containing tetracycline antibiotics and a potato dextrose agar culture medium to obtain a culture growth substance; and the culture growth substance is subcultured on fresh plates at 30 DEG C until a single fungus strain is obtained on each plate. The fungus strain A.flavus G10 is high in speed of degrading the polyurethane plastic.