75 results about "Sphingomonas azotifigens" patented technology

The present invention relates to a Lactuca sativa L. var. longifolia Lam seed designated as 41-49 RZ, which has leaves with a hollow main vein, broad base leaves, and exhibits resistance against downy mildew (Bremia lactucae Regel), currant-lettuce aphid (Nasonovia ribisnigri), corky root (Sphingomonas suberifaciens) and lettuce mosaic virus (LMV). The present invention also relates to a Lactuca sativa L. var. longifolia Lam plant produced by growing the “41-49 RZ” seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 41-49 RZ.

The invention relates to Sphingomonas sp. TP-5 and a method and an application of the same for producing welan gum. A strain of the invention is prepared by separating and acclimating sugar-contained wastewater in a molasses plant, has a systematic name of Sphingomonas sp. and a preservation number of CGMCCNo.3097, not only can grow in nutrient media of beef broth, LB and nutrient agar, but also can grow in a sugar-contained inorganic-salt culture medium. The TP-5 strain can be fermented in the sterile culture medium containing sugar, inorganic salt and water at a temperature condition of 28-37 DEG C; ymotic fluid is extracted to obtain the welan gum achieving a temperature-resistant limit value of 150 DEG C; and potassium chloride in different concentrations is added so that a welan gum solution remarkably improves the viscosity, enhances the temperature stability, does not vary the viscosity with temperature and presents stronger temperature tolerance, thereby being applied to seawater drilling mud and a high salinity reservoir viscous water flooding system.

The present invention relates to a Lactuca sativa L. var. longifolia Lam seed designated as 41-52 RZ, referred to as Nirvanus, which exhibits resistance against downy mildew (Bremia lactucae Regel), currant-lettuce aphid (Nasonovia ribis-nigri), corky root (Sphingomonas suberifaciens) and lettuce mosaic virus (LMV), the representative seed having been deposited with the National Collections of Industrial, Marine and Food Bacteria (NCIMB) in Bucksburn, Aberdeen AB21 9YA, Scotland, UK and assigned NCIMB Acession No. 41326. The present invention also relates to a Lactuca sativa L. var. longifolia Lam plant produced by growing the 41-52 RZ (Nirvanus) seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 41-52 RZ.

The present invention relates to a Lactuca sativa seed designated 41-177 RZ, which exhibits a combination of traits including resistance against downy mildew (Bremia lactucae) races Bl:1-28 and Ca-I to Ca-VIII, currant-lettuce aphid (Nasonovia ribisnigri) biotype Nr:0, lettuce mosaic virus (LMV), and corky root rot (Sphingomonas suberifaciens), as well as solid main veins and narrow base leaves. The present invention also relates to a Lactuca sativa plant produced by growing the 41-177 RZ seed. The invention further relates to methods for producing the lettuce cultivar, represented by lettuce variety 41-177 RZ.

The invention belongs to the technical field of microbial strains and in particular relates to a sphingomonas strain xp. The sphingomonas strain xp is classified and named as Sphingomonas sp., and the collection number of the strain in the China center for type culture collection (CCTCC) is CCTCC No:M 2011453. The invention further relates to an application of the sphingomonas strain xp to degradation of phenolic compounds in tobacco products, and the degradation effect is good.

The present invention relates to a co-culture method of Sphingomonas sp. bacterial strain and Aspergillus sp. fungus strain, in which the novel Sphingomonas sp. bacterial strain KMK-001 is cultured in a liquid medium and the novel Aspergillus sp. strain KMC-901 separately cultured in another liquid medium is added to the above culture solution, a novel glionitrin biosynthesized therefrom and a pharmaceutical composition comprising the said glionitrin or its pharmaceutically acceptable salt as an active ingredient. The glionitrin herein has strong cytotoxic effect on cancer cells and has antibiotic effect on 10 pathogenic bacteria including the novel Sphingomonas sp. bacterial strain KMK-001, so that it can be effectively applied in antibiotics or anti-cancer agents.

The invention discloses astaxanthin synthetase of sphingomonas, an encoding gene of astaxanthin synthetase and a method for genetic manipulation of sphingomonas. The invention verifies that the sphingomonas can be used for producing astaxanthin, a biological synthesis way capable of producing the astaxanthin is contained, an MEP way and a carotenoid synthesis way are used for producing the astaxanthin in the sphingomonas, and the fact that in the sphingomonas, crtE and crtZ having relatively low homology with the known genes have the function of synthesizing the astaxanthin, can be further proven. Both the enzyme and the encoding gene thereof required by the astaxanthin biosynthetic way of the sphingomonas are not reported in the prior art, so that more resources are provided to the biosynthetic metabolism and transformation of the carotenoid. Genetic manipulation can be carried out on the sphingomonas by taking pBBR1MCS2 as a carrier and taking EGFP as a reporter gene, so that a foundation is laid for the genetic modification of the environmental microorganism of the type and the finding of the degradation mechanisms.

The invention provides a composite immobilized microbial agent as well as a preparation method and application thereof. The preparation method comprises the following steps of preparing a bacterial suspension of petroleum hydrocarbon degrading bacteria, wherein the petroleum hydrocarbon degrading bacteria comprise one or a combination of more of a pseudomonas pseudoalcaligenes strain, a pseudomonas guguanensis strain, am acinetobacter venetianus and a sphingobacterium pakistanense strain; adding biochar into the bacterial suspension, and uniformly mixing to enable the petroleum hydrocarbon degrading bacteria to be fully adsorbed on the biochar, so as to obtain a biochar-bacterial liquid mixed solution; and uniformly mixing a mixed NaCl aqueous solution of sodium alginate and polyvinyl alcohol with the biochar-bacterial liquid mixed solution to carry out cross-linking immobilization, dropwise adding the obtained mixed solution into a CaCl2-boric acid solution, cleaning the obtained mixed solution with the NaCl aqueous solution, placing the mixed solution on a sterile culture medium plate, and naturally airing. The method is simple and easy to implement, low in cost and high in operability; and the obtained composite immobilized microbial agent has excellent mechanical strength and excellent degradation effect on petroleum hydrocarbon.

The invention belongs to identification of microorganisms, in particular to a molecular marker used for identification of Sanzan gum and synthesis bacteria thereof, and preparation and application ofthe molecular marker. Sphingomonas sp.CGMCC No.1650 or genome DNA of CGMCC No.19480 is used as a PCR amplification formwork, a primer 1 having a sequence being SEQ No.5 and a primer 2 having a sequence being SEQ No.6 are used as primers, PCR amplification is performed, and the molecular marker is obtained. The problems that when a compounding glue system of the Sanzan gum, gellan gum and welan gumis used, only through other operations of cloning and sequencing and the like, the three gum bodies can be mutually separated, and the step is tedious, can be solved. The molecular marker has the advantages that the Sanzan gum can be identified from medium and light processed products containing the Sanzan gum, and is short in detection time and high in accuracy.

The invention relates to a method for degrading a petroleum component by utilizing nano bamboo charcoal reinforcing functional microorganism and application of the method. The functional microorganism is sphingomonas sp. GY2B; the petroleum component includes polycyclic aromatic hydrocarbon (phenanthrene) and crude oil; 50 to 200mg / L of nano bamboo charcoal can be added to obviously improve the degrading capacity of the functional strain GY2B on petroleum component in water; the sodium alginate embedding method is adopted to cure nano bamboo charcoal and the functional microorganism GY2B to be applied to biological treatment of wastewater containing petroleum component and biological repair of water polluted by petroleum.

The invention provides a sphingomonas engineering bacterium and its construction method and application. The present invention uses Sphingomonas sp.T-3 as the starting strain, utilizes the gene overexpression method to realize the high-efficiency transcription of three key genes in the synthesis of sanzan gum, constructs the engineering strain MH-10, and effectively improves the microbial polysaccharide by optimizing the fermentation conditions. (Sanzan glue) has a wide range of industrial application value.

The invention belongs to the technical field of microorganisms strains, and in particular relates to a sphingomonas strain xp, wherein the sphingomonas strain xp is classified and named as (i) Sphingomonas ( / i) sp (i). ( / i); the preservation number of the strain xp in China Center for Type Culture Collection is CCTCC No: M2011453. The sphingomonas strain xp is applied to degrading polyphenolic compounds in tobacco stems, and is good in degradation effect.