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Sphingomonas zeae and application thereof in biological deodorization

A sphingomonas and biological deodorization technology, applied in the biological field, can solve the problems of high equipment cost, secondary pollution and high operation requirements, and achieve the effects of high deodorization efficiency, good application potential and obvious degradation effect.

Active Publication Date: 2021-01-01
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical methods and chemical methods are suitable for occasions where the concentration of odor pollutants is high, and have the advantages of high odor treatment efficiency, short treatment residence time, and fast start-up; Demanding high disadvantages

Method used

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  • Sphingomonas zeae and application thereof in biological deodorization
  • Sphingomonas zeae and application thereof in biological deodorization
  • Sphingomonas zeae and application thereof in biological deodorization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 strain screening and identification

[0032] 1. Pick out bones, napkins and other substances from the kitchen waste in Yuxiu Canteen of Zhejiang University of Technology, and then use a crusher to crush the remaining waste.

[0033] 2. Take 1g of crushed food waste and dilute it to 1×10 with ultrapure water -6 , 1×10 -7 , 1×10 -8 , and take 200 μL of each dilution to solid LB medium, spread evenly with a spreader stick, place in a constant temperature incubator at 28°C for 48h to 72h, pick a single colony for streak purification;

[0034] The formula of LB medium is: yeast powder 5g, peptone 10g, sodium chloride 5g, agar 15g.

[0035] 3. After multiple rounds of purification and screening, the strains with bright yellow color, smooth shape and no wrinkles were selected, and single colonies were picked to extract the genome with the FastDNA Spin Kit for Soil kit.

[0036] 4. Use the extracted genome as a template for PCR. The PCR system is: 1 μL of template...

Embodiment 2

[0038] The preparation of embodiment 2 biological deodorizing bacteria agents

[0039] 1. Insert the Sphingomonas zea strains screened in Example 1 into the LB medium.

[0040] 2. Inoculate the activated strains into LB liquid medium for shaking culture (37°C, 180r / min) for more than 12 hours.

[0041] 3. Count the cultured bacterial solution and adjust the strain content to 10 8 ~10 9 CFU / ml.

[0042] 4. Centrifuge the bacterial solution (12000r / min, 10min), discard the supernatant after centrifugation, and collect the bacterial cells.

[0043] 5. Mix the bacteria with diatomaceous earth, wheat straw, and corncobs at a ratio of 1:1 (w / w), and then place them in a 37°C incubator until they are dry and constant weight, which are various bacterial agents. Diatomaceous earth is a molecular-grade product purchased in the market; wheat straw and corncobs are pre-treated and then mixed with bacterial agents. ℃ drying. The bacterial agents with diatomite, wheat straw and corn c...

Embodiment 3

[0048] Example 3 Carry out deodorization test with kitchen waste as substrate

[0049] The fresh kitchen waste was crushed with a blender and distributed into 500ml Erlenmeyer flasks. Each shaker bottle was filled with 100g (wet weight) of fresh food waste. Three parallel experiments were set up for each group of experiments. Then the bacterial agent prepared in Example 2 was added in an amount of 10 g / bottle, and only 100 g of fresh kitchen waste was added as a blank control. Then seal it with three layers of plastic wrap, and tie the mouth of the bottle tightly with a rubber band. Place in a constant temperature box at 28°C, and measure the concentrations of ammonia and hydrogen sulfide after 24 hours.

[0050] After three groups of parallel experiments, it was found that the degradation rate of hydrogen sulfide in kitchen waste was 87.65%, and the degradation rate of ammonia gas was 19.91% after inoculation of biological deodorizing bacteria agent for 24 hours. From the a...

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Abstract

The invention discloses Sphingomonas zeae E18 and an application thereof in biological deodorization, and belongs to the technical field of biology. The preservation number of the Sphingomonas zeae E18 is CCTCC NO: M 2020193. The strain is high in deodorization efficiency, and a biological deodorization microbial agent prepared from the Sphingomonas zeae E18 has obvious degradation effects on odors generated by restaurant and kitchen waste, and has relatively good application potential.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a sphingomonas zea and its application in biological deodorization. Background technique [0002] According to the World Food and Agriculture Organization, nearly one-third of the food produced in the world each year (about 1.3 billion tons) is lost or wasted in the food supply chain. Due to the high moisture content and high organic matter content, food waste is very prone to corruption, resulting in odor emission and breeding of insects and flies. [0003] Stench is a general term for all gases that stimulate the olfactory organs to cause people's unpleasantness and damage the human living environment. Stench is one of the seven major public hazards recognized in the world, second only to noise. The stench can cause nausea, dizziness, vomiting and other reactions in people, and it can also cause human respiratory, cardiovascular and other diseases in severe cases. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B3/00C12R1/01
CPCC12N1/20B09B3/00C12R2001/01C12N1/205
Inventor 薛亚平丁亦然邹树平郑裕国周海岩柯霞
Owner ZHEJIANG UNIV OF TECH
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