Composite immobilized microbial agent as well as preparation method and application thereof

Abstract

The invention provides a composite immobilized microbial agent as well as a preparation method and application thereof. The preparation method comprises the following steps of preparing a bacterial suspension of petroleum hydrocarbon degrading bacteria, wherein the petroleum hydrocarbon degrading bacteria comprise one or a combination of more of a pseudomonas pseudoalcaligenes strain, a pseudomonas guguanensis strain, am acinetobacter venetianus and a sphingobacterium pakistanense strain; adding biochar into the bacterial suspension, and uniformly mixing to enable the petroleum hydrocarbon degrading bacteria to be fully adsorbed on the biochar, so as to obtain a biochar-bacterial liquid mixed solution; and uniformly mixing a mixed NaCl aqueous solution of sodium alginate and polyvinyl alcohol with the biochar-bacterial liquid mixed solution to carry out cross-linking immobilization, dropwise adding the obtained mixed solution into a CaCl2-boric acid solution, cleaning the obtained mixed solution with the NaCl aqueous solution, placing the mixed solution on a sterile culture medium plate, and naturally airing. The method is simple and easy to implement, low in cost and high in operability; and the obtained composite immobilized microbial agent has excellent mechanical strength and excellent degradation effect on petroleum hydrocarbon.

Description

technical field [0001] The invention relates to a composite immobilized bacterial agent and its preparation method and application, belonging to the technical field of environmental bioremediation. Background technique [0002] As one of the world's three major energy sources, oil is of great importance to human beings. However, oil leakage is inevitable in the process of oil exploitation and utilization. Due to the complex composition of oil pollution, poor fluidity, and serious biological toxicity, it will affect water bodies and soils. and serious harm to human health. Oil leakage into the soil will cause the most serious pollution to the surface soil, which can destroy the structure of the soil, causing problems such as poor soil permeability, adhesion and hardening of soil particles, and soil salinization. In addition, after oil pollutes the soil, the carbon content in the soil will increase, while the nitrogen and phosphorus content will remain unchanged, so the carbo...

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Problems solved by technology

At present, the immobilized microbial technology only immobilizes one kind of highly efficient oil-degrading bacteria. For example, only one kind of bacteria is used in the immobilized bacterial agent disclosed in Chinese patent CN106148318A (application number: 201610524938.2).

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