60 results about "Sphingobacterium" patented technology

Butane-utilizing bacteria are used to degrade hydrocarbon pollutants such as trichloroethene (TCE). In-situ or ex-situ techniques may be used to reduce or eliminate hydrocarbon pollutants from liquid, gas and solid sources. In a preferred embodiment, TCE concentrations in various aqueous environments are reduced by contacting a contaminated water source with butane-utilizing bacteria in the presence of oxygen to degrade the TCE by cometabolism or direct metabolism. Suitable butane-utilizing bacteria include Pseudomonas, Variovorax, Nocardia, Chryseobacterium, Comamonas, Acidovorax, Rhodococcus, Aureobacterium, Micrococcus, Aeromonas, Stenotrophomonas, Sphingobacterium, Shewanella, Phyllobacterium, Clavibacter, Alcaligenes, Gordona, Corynebacterium and Cytophaga. The butane-utilizing bacteria have relatively low TCE toxicity in comparison with conventional methane-utilizing bacteria, and demonstrate an improved ability to degrade TCE.

Butane-utilizing bacteria are used to degrade hydrocarbon pollutants such as trichloroethene (TCE). In-situ or ex-situ techniques may be used to reduce or eliminate hydrocarbon pollutants from liquid, gas and solid sources. In a preferred embodiment, TCE concentrations in various aqueous environments are reduced by contacting a contaminated water source with butane-utilizing bacteria in the presence of oxygen to degrade the TCE by cometabolism or direct metabolism. Suitable butane-utilizing bacteria include Pseudomonas, Variovorax, Nocardia, Chryseobacterium, Comamonas, Acidovorax, Rhodococcus, Aureobacterium, Micrococcus, Aeromonas, Stenotrophomonas, Sphingobacterium, Shewanella, Phyllobacterium, Clavibacter, Alcaligenes, Gordona, Corynebacterium and Cytophaga. The butane-utilizing bacteria have relatively low TCE toxicity in comparison with conventional methane-utilizing bacteria, and demonstrate an improved ability to degrade TCE.

A process for preparing heparin oligose from heparinase includes such steps as culturing sphingobacterium (CGMCC No.0660), preparing non-cell coarse enzyme liquid, extracting and purifying heparinase, degradating heparin by the heparinase at 20-30 deg.C to obtain heparin oligose mixture, ultrafilter, gel filter for fractionation and evaporation concentration. The product has the activity of resisting smooth muscle hyperplasia.

DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

The invention relates to a preparation method for an amendment used for restoration of petroleum-heavy metal combinedly-contaminated saline soil. The preparation method comprises the following steps: airing carbon black to obtain a black powder or particle; preparing 8*10<10> / ml of a composite strain from bacillus subtilis and sphingobacterium multivolume in a ratio of 1: 1; air drying a biogas residue, crushing the biogas residue, sieving the crushed biogas residue with a 10-mesh sieve and compounding the biogas residue with carbon black, urea and a high efficiency petroleum degrading bacterium according to a certain proportion; and carrying out flat-mold type extrusion and granulation to produce a cylindrical particle, rounding the cylindrical particle by using a rounding machine, then screening the particle with a vibrating screen, allowing the particle to enter into a cooler for cooling and then carrying out weighing and bagging. The amendment prepared by using the method provided by the invention can reduce activity of heavy metals in the degradation process of petroleum while realizing high efficiency degradation of petroleum hydrocarbons in soil by using the petroleum degrading bacterium, accelerates growth of plants grown in saline soil and promotes micro-biological degradation efficiency by using the plants. The method provides a highly efficient, economic and safe amendment for microbe-plant restoration of mild petroleum-heavy metal contaminated soil.

A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.

The invention discloses Sphingobacterium (sp.) SIT102 with the preservation number of CGMCC NO.6158 and a method for performing enantioselective hydrolysis to generate levetiracetam acid by utilizing the Sphingobacterium SIT102 serving as biocatalyst to catalyze racemization etiracetam acid esters. The method for preparing the levetiracetam acid comprises the steps of placing Sphingobacterium SIT102 cells into buffered solution, adding the racemization etiracetam acid esters into the buffered solution, performing the enantioselective hydrolysis through catalysis to obtain the levetiracetam acid. According to the method for preparing the levetiracetam acid by utilizing the Sphingobacterium SIT102 serving as the biocatalyst, the used biocatalyst is easy to prepare, the reaction condition is moderate, the yield of the levetiracetam acid can reach 48%, the enantiomer excess reaches 96%, and the production cost is low. Therefore, the method for preparing the levetiracetam acid by utilizing the Sphingobacterium has considerable industrial application development prospects.

The invention relates to a mixed microbial preparation for recovering soil polluted by composite pollutant oil in a biology manner and a manufacture method of the mixed microbial preparation. The mixed microbial preparation is made by mixing six bacterial strains including acinetobacter calcoaceticus, klebsiella pneumonia, enterobacter cancerogenus, pseudomonas stutzeri, rhodococcus erythropolis, and pseudomonas putida with one of rhodococcus, acinetobacter, gordonia, sphingobacterium, novosphingobium capsulatum, acinetobacter, and xanthomonas at a ratio of 1:1:2:2:1:1:1:1 and is used for recovering oil-polluted soil.

The invention relates to a slow-release microbial inoculum and a preparation method and application thereof.Sewage denitrification treatment is achieved through symbiotic coordination among bacillus megatherium, sphingobacterium and bacillus mucilaginosus, a large specific surface area is provided for microorganisms through a special framework carrier, the stability of the microorganisms in the carrier is guaranteed, and the slow-release microbial inoculum is prepared. The embedding medium formed by compounding polyvinyl alcohol, sodium alginate, agar and gelatin provides a stable and low-viscosity film to wrap microorganisms, the release speed of the microorganisms is delayed, meanwhile, the loading success rate of the microorganisms can be increased, part of skeleton carrier components can absorb moisture and expand, the microorganisms are helped to be released, and the stability of the microorganisms is improved. The long-term activity of microorganisms is guaranteed through an appropriate slow-release carbon source, the overall mechanical performance of the slow-release microbial agent is improved through interaction of all the components, the carrier breaking speed is reduced, wetland blockage can be relieved when the slow-release microbial agent is used for a wetland, and the long-term stability of the sewage treatment effect is guaranteed. The preparation process of the slow-release microbial inoculum is simple, and industrial production can be realized.

The invention belongs to the field of bioremediation of organic pollutants, and specifically discloses a Sphingobacterium sp. strain W144. The strain is preserved in China Center for Type Culture Collection, and has a preservation number of CCTCC NO:M2014151. The strain disclosed by the invention has rapid and efficient degradation ability on phenanthrene, biphenyl, pyrene, fluorene, fluoranthene and other organic pollutants, has a wide range of pH value, and salinity and temperature adaptation ability, and is a good material for bioremediation of polycyclic aromatic hydrocarbons in soil or the environment.

The invention provides a composite immobilized microbial agent as well as a preparation method and application thereof. The preparation method comprises the following steps of preparing a bacterial suspension of petroleum hydrocarbon degrading bacteria, wherein the petroleum hydrocarbon degrading bacteria comprise one or a combination of more of a pseudomonas pseudoalcaligenes strain, a pseudomonas guguanensis strain, am acinetobacter venetianus and a sphingobacterium pakistanense strain; adding biochar into the bacterial suspension, and uniformly mixing to enable the petroleum hydrocarbon degrading bacteria to be fully adsorbed on the biochar, so as to obtain a biochar-bacterial liquid mixed solution; and uniformly mixing a mixed NaCl aqueous solution of sodium alginate and polyvinyl alcohol with the biochar-bacterial liquid mixed solution to carry out cross-linking immobilization, dropwise adding the obtained mixed solution into a CaCl2-boric acid solution, cleaning the obtained mixed solution with the NaCl aqueous solution, placing the mixed solution on a sterile culture medium plate, and naturally airing. The method is simple and easy to implement, low in cost and high in operability; and the obtained composite immobilized microbial agent has excellent mechanical strength and excellent degradation effect on petroleum hydrocarbon.

The invention discloses a preparation method of a microbial agent for plant disease prevention and cure, and relates to the technical field of microbial prevention and cure of plant diseases. The steps are as below: (1) preparing the following raw materials: Paecilomyces lilacinus secondary bacterial fluid, Bacillus licheniformis-Sphingobacterium mixed fermentation fluid, Gram-positive actinomycetes-Bacillus cereus mixed fermentation fluid, nitrogen-fixing bacteria, Bacillus megaterium, photosynthetic bacteria, amino acids, humic acids, monosaccharides, chitin and modified diatomaceous earth;(2) preparing the Bacillus licheniformis-Sphingobacterium mixed fermentation fluid; (3) preparing the Gram-positive actinomycetes-Bacillus cereus mixed fermentation fluid; (4) preparing a mixed bacterial fluid A; and (5) adding the remaining raw materials to the mixed bacterial fluid A to obtain the microbial agent for plant disease prevention and cure. According to the present invention, the microbial agent contains a plurality of beneficial bacteria species, is effective for eliminating root-knot nematodes and eggs in soil by inhibiting bacteria with bacteria and by killing pests with bacteria, and is effective for inhibiting the growth and reproduction of soil-borne pathogenic bacteria.

The invention discloses Sphingobacterium multivorum and an application thereof. The Sphingobacterium multivorum is Sphingobacterium multivorum P6, the Sphingobacterium multivorum P6 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is May 31, 2021, and the preservation number is CCTCC NO: M2021648. The strain can convert insoluble inorganic phosphate into soluble phosphorus which can be directly absorbed and utilized by plants, the phosphorus solubilizing amounts of the strain cultured in four insoluble phosphate liquid culture media including calcium phosphate, iron phosphate, aluminum phosphate and ground phosphate rock for 7 days are 47.2 [mu]g / mL, 6.1 [mu]g / mL , 3.8 [mu]g / mL and 9.5 [mu]g / mL respectively, and a certain reference is provided for later research of wild strains. The Sphingobacterium multivorum has an important value for development of microbial fertilizers such as phosphate-solubilizing bacterial fertilizers and the like.

The invention discloses sphingobacterium multivorum and application thereof, and belongs to the technical field of bioengineering and environmental engineering. The sphingobacterium multivorum is collected in the China General Microbiological Culture Collection Center (CGMCC) on October 17, 2018 at the collection number of CGMCC No. 16596. The strain has heterotrophic nitrification and aerobic denitrification characteristics, can be applied to the field of nitrogen-containing sewage treatment and river treatment, has an ammonia nitrogen removal rate more than 90 percent, is pollution-free to the environment, achieves effects of quickly biologically removing nitrogen and recovering the self-cleaning capacity of a river, and has great significance for the field of environmental water treatment.

The invention relates to sphingobacterium sp., the classification name of the sphingobacterium sp. Is sphingobacterium sp., and the preservation number of the sphingobacterium sp. Is CGMCC (China General Microbiological Culture Collection Center) NO.18204. The sphingobacterium sp. The strain can be used for effectively degrading tetracycline antibiotics, and is of great significance for reducing environmental pollution and ecological imbalance caused by antibiotics.

The invention belongs to the biotechnological field and discloses a Sphingobacterium multivorum and a method for conversion of the Sphingobacterium multivorum into validamycin. The strain or thallus of the strain can convert the validamycin into valid amine and valid enamine; after being cultured in a seed culture medium, the strain or the thallus of the strain is inoculated into a transformation culture medium which contains substrate validamycin for decomposition of the substrate, or the thallus is obtained by separation after being cultured in the seed culture medium and the thallus obtained is added into validamycin solution for decomposition of the substrate; after separation and purification of decomposition liquor, the valid amine and the valid enamine are obtained. The invention also discloses a detection and separation method for the valid amine and the valid enamine. The strain Sphingobacterium multivorum is well-grown in the culture medium which contains the validamycin and the solution culture and transformation effect is good; moreover, the strain has the advantages of innocuity, no pathogenicity, stable performance, difficulty in contamination and so on.

The invention discloses a mixed bacterial flora for degrading fumonisin B1 and a crude enzyme preparation thereof. The mixed bacterial flora for degrading fumonisin B1 comprises the following components: Pseudomonas (Pseudomonas), Chaetomonas (Comamonas) and Sphingobacterium (sphingobacterium), wherein the total effective viable number of the mixed microflora accounts for more than 90% of the total number of the mixed microflora. The mixed bacterial flora degrading fumonisin B1 of the invention is amplified and cultured in MM culture medium containing sucrose, the bacterial body obtained by centrifugation is broken by ultrasonic wave, and the supernatant is lyophilized to obtain crude enzyme preparation. The mixed bacterial flora degrading fumonisin B1 of the invention is amplified and cultured in MM culture medium containing sucrose. The fumonisin B1 degradation rate of the mixed microflora and the crude enzyme preparation thereof obtained by the invention is above 90% in 24 hours. The mixed microbial flora and its crude enzyme preparation have the characteristics of simple fermentation and low production cost, and have broad application prospects for detoxification of fumonisin B1 in feedstuffs.

The invention discloses a slow-release bacterial fertilizer as well as a preparation method and application thereof, and relates to the technical field of biotechnology. Comprising the following steps: step 1, preparing a bacterial liquid of bacillus cereus, a bacterial liquid of lysinibacillus slender, a bacterial liquid of acinetobacter schiffei, a bacterial liquid of stenotrophomonas maltophilia, a bacterial liquid of sphingobacterium alcohovorans and a bacterial liquid of bacillus licheniformis into a mixed bacterial suspension according to a volume ratio of 1: (1-2): (1-2): (1-2): (1-2): (1-2); 2, mixing the mixed bacterium suspension with an embedding agent and a cross-linking agent to obtain a first mixed colloid; step 3, embedding the first mixed colloid into the second mixed layer to obtain first compound fertilizer particles; and step 4, embedding the first compound fertilizer particles into a fermented third mixed layer to obtain the slow-release bacterial fertilizer. The prepared slow-release bacterial fertilizer improves the remediation effect of organic phosphorus contaminated soil. The soil fertility is improved, and a good growth promoting effect on plants is achieved.

The invention provides a compound microbial agent and application thereof in regulating aquaculture water quality, and the compound microbial agent is prepared by the following steps: firstly, embedding a bacterial liquid composed of alcaligenes faecalis, pseudomonas, ochrobactrum and sphingosine box bacteria through self-assembly by adopting gallic acyl quinic acid, and then modifying lignin by using ferrocene for composite loading. And immersing into a bacterial liquid consisting of photosynthetic bacteria, bacillus and nitrifying bacteria, and drying. According to the invention, ferrocene modified lignin and galloylquinic acid construct a multi-layer load structure to fix microorganisms in a layered manner, so that the microorganisms can stably survive in a complex water body environment, and meanwhile, a plurality of florae act synergistically to degrade organic matters, nitrogen, phosphorus, antibiotics and the like in aquaculture water, so that the degradation efficiency of water pollutants can be remarkably improved; effective adjustment of aquaculture water quality is facilitated, and good data support and theoretical basis are provided for water body improvement in the field of aquatic products.

The invention relates to a preparation method for an amendment used for restoration of petroleum-heavy metal combinedly-contaminated saline soil. The preparation method comprises the following steps: airing carbon black to obtain a black powder or particle; preparing 8*10<10> / ml of a composite strain from bacillus subtilis and sphingobacterium multivolume in a ratio of 1: 1; air drying a biogas residue, crushing the biogas residue, sieving the crushed biogas residue with a 10-mesh sieve and compounding the biogas residue with carbon black, urea and a high efficiency petroleum degrading bacterium according to a certain proportion; and carrying out flat-mold type extrusion and granulation to produce a cylindrical particle, rounding the cylindrical particle by using a rounding machine, then screening the particle with a vibrating screen, allowing the particle to enter into a cooler for cooling and then carrying out weighing and bagging. The amendment prepared by using the method provided by the invention can reduce activity of heavy metals in the degradation process of petroleum while realizing high efficiency degradation of petroleum hydrocarbons in soil by using the petroleum degrading bacterium, accelerates growth of plants grown in saline soil and promotes micro-biological degradation efficiency by using the plants. The method provides a highly efficient, economic and safe amendment for microbe-plant restoration of mild petroleum-heavy metal contaminated soil.

The present invention belongs to the field of microorganisms, and specifically discloses a strain of Sphingobacterium shellycum and its application. The Sphingobacterium shellycum (Sphingobacteriumputeale) strain TYQ6 was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on April 27, 2023, with a deposit number of CGMCC.27215. The applicant found through experiments that the above-mentioned Sphingobacterium shellycum can secrete auxin, which can promote the growth of cucumber seedlings. It also has the ability to enhance the cucumber seedlings' ability to resist drought, interfere with the tropism migration of nematodes, and kill nematodes; therefore, the above-mentioned Sphingobacterium shellycum has broad application prospects in agricultural production.

The invention discloses a strain for efficiently degrading doxycycline in a water body and application of the strain. The Sphingobacterium hotanense DX14 screened by the invention can be used for efficiently degrading the doxycycline in the water body, so that the strain has the potential of being developed into a microbial agent for degrading the doxycycline in the water body, and has a good application prospect for solving the problems that the doxycycline in the antibiotic-containing water body exceeds the standard and the like. Meanwhile, the invention further provides an environment-friendly application method of the sphingobacterium mesophilum DX14, the sphingobacterium mesophilum DX14 is recovered, and genome DNA of the sphingobacterium mesophilum DX14 is completely degraded, so that the doxycycline resistance gene carried by the sphingobacterium mesophilum DX14 is effectively prevented from being diffused in a water body, and the yield of the doxycycline resistance gene is improved. And the effect of comprehensively protecting the environment is achieved.

The invention discloses a microorganism-containing composite soil remediation agent which comprises a composite microbial agent and composite powder, wherein the composite microbial agent comprises bacillus, sphingobacterium, pseudomonas and aspergillus oryzae, and the composite powder comprises diatomite, aquatic floaters, livestock and poultry manure, bagasse and corn straw. The composite powder is prepared from the following components in parts by weight: 20 to 40 parts of diatomite, 10 to 20 parts of aquatic floaters, 5 to 10 parts of livestock and poultry manure, 3 to 10 parts of bagasse and 20 to 30 parts of corn straws. Various production wastes obtained by agricultural production are adopted as raw materials, the manufacturing cost can be remarkably reduced, and due to the adoption of natural raw materials, secondary damage to soil is avoided, the remediation effect is excellent, and the soil remediation agent is more natural and environmentally friendly.

The invention belongs to the field of astragalus growth-promoting technology, and provides astragalus growth-promoting endophytic bacteria and its growth-promoting method and application in order to solve the current problems of astragalus membranaceus growth promotion and quality improvement. Promoting endophytes are: Sphingobacterium ( Sphingobacterium sp. ) strain KSCO1 and Pseudomonas phrysalis ( Pseudomonas poae ) strain KSCO2; on November 21, 2017, it was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation numbers are: CGMCC No.14945, CGMCC No.14946. 2~10μM E‑2 ‑Hexenal and 5-625 μM n-hexanol promoted the growth of KSC01, and 25-625 μM n-hexanol promoted the growth of KSCO2. A green, environment-friendly pesticide component with no medicinal material residue is provided for the cultivation of Chinese medicinal material Astragalus membranaceus.

The invention belongs to the technical field of sewage treatment, and particularly relates to a preparation method of a low-temperature composite flora, a cold-resistant microbial agent for low-temperature sewage treatment and application of the cold-resistant microbial agent. The invention provides a low-temperature complex microbial community, which is prepared from flavobacterium (Flavobacterium), aeromonas (Aeromonas), sphingomonas (Sphingobacterium), pseudomonas (Pseudomonas), Comamonas (Comamonas), Massiella (Massiella) and Delftia (Delftia), and is characterized in that the low-temperature complex microbial community is prepared from the following raw materials: the flavobacterium (Flavobacterium), the aeromonas (Aeromonas), the sphingomonas (Sphingobacterium), the pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas (Pseudomonas The low-temperature composite flora can treat domestic wastewater under a low-temperature condition, and is high in nitrogen removal rate, short in treatment time, wide in application range and better in impact resistance.

The invention discloses a sphingobacterium sp. And an application thereof. The sphingobacterium sp. Disclosed by the invention is preserved in the China Center for Type Culture Collection on May 7, 2025, and the preservation number of the sphingobacterium sp. Is CCTCC (China Center for Type Culture Collection) NO: M 2025987. The sphingobacterium sp. Disclosed by the invention can be used for treating and repairing polycyclic aromatic hydrocarbons (phenanthrene, fluoranthene and pyrene) in soil, water bodies or sediments under heavy metal stress, and has a relatively wide application prospect. The degradation rate of the sphingobacterium sp. To inorganic salt culture media containing 25 mg / L of phenanthrene, 25 mg / L of fluoranthene and 25 mg / L of pyrene can reach 80%-85% within 10 days, the tolerance of the sphingobacterium sp. To heavy metal is high, and the degradation rate range can reach 40%-85% under the condition that pollution of 0-100 mg / L of Cd < 2 + > exists at the same time.