39 results about "Sphingobacterium" patented technology

Butane-utilizing bacteria are used to degrade hydrocarbon pollutants such as trichloroethene (TCE). In-situ or ex-situ techniques may be used to reduce or eliminate hydrocarbon pollutants from liquid, gas and solid sources. In a preferred embodiment, TCE concentrations in various aqueous environments are reduced by contacting a contaminated water source with butane-utilizing bacteria in the presence of oxygen to degrade the TCE by cometabolism or direct metabolism. Suitable butane-utilizing bacteria include Pseudomonas, Variovorax, Nocardia, Chryseobacterium, Comamonas, Acidovorax, Rhodococcus, Aureobacterium, Micrococcus, Aeromonas, Stenotrophomonas, Sphingobacterium, Shewanella, Phyllobacterium, Clavibacter, Alcaligenes, Gordona, Corynebacterium and Cytophaga. The butane-utilizing bacteria have relatively low TCE toxicity in comparison with conventional methane-utilizing bacteria, and demonstrate an improved ability to degrade TCE.

Butane-utilizing bacteria are used to degrade hydrocarbon pollutants such as trichloroethene (TCE). In-situ or ex-situ techniques may be used to reduce or eliminate hydrocarbon pollutants from liquid, gas and solid sources. In a preferred embodiment, TCE concentrations in various aqueous environments are reduced by contacting a contaminated water source with butane-utilizing bacteria in the presence of oxygen to degrade the TCE by cometabolism or direct metabolism. Suitable butane-utilizing bacteria include Pseudomonas, Variovorax, Nocardia, Chryseobacterium, Comamonas, Acidovorax, Rhodococcus, Aureobacterium, Micrococcus, Aeromonas, Stenotrophomonas, Sphingobacterium, Shewanella, Phyllobacterium, Clavibacter, Alcaligenes, Gordona, Corynebacterium and Cytophaga. The butane-utilizing bacteria have relatively low TCE toxicity in comparison with conventional methane-utilizing bacteria, and demonstrate an improved ability to degrade TCE.

A process for preparing heparin oligose from heparinase includes such steps as culturing sphingobacterium (CGMCC No.0660), preparing non-cell coarse enzyme liquid, extracting and purifying heparinase, degradating heparin by the heparinase at 20-30 deg.C to obtain heparin oligose mixture, ultrafilter, gel filter for fractionation and evaporation concentration. The product has the activity of resisting smooth muscle hyperplasia.

DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

The invention relates to a preparation method for an amendment used for restoration of petroleum-heavy metal combinedly-contaminated saline soil. The preparation method comprises the following steps: airing carbon black to obtain a black powder or particle; preparing 8*10<10> / ml of a composite strain from bacillus subtilis and sphingobacterium multivolume in a ratio of 1: 1; air drying a biogas residue, crushing the biogas residue, sieving the crushed biogas residue with a 10-mesh sieve and compounding the biogas residue with carbon black, urea and a high efficiency petroleum degrading bacterium according to a certain proportion; and carrying out flat-mold type extrusion and granulation to produce a cylindrical particle, rounding the cylindrical particle by using a rounding machine, then screening the particle with a vibrating screen, allowing the particle to enter into a cooler for cooling and then carrying out weighing and bagging. The amendment prepared by using the method provided by the invention can reduce activity of heavy metals in the degradation process of petroleum while realizing high efficiency degradation of petroleum hydrocarbons in soil by using the petroleum degrading bacterium, accelerates growth of plants grown in saline soil and promotes micro-biological degradation efficiency by using the plants. The method provides a highly efficient, economic and safe amendment for microbe-plant restoration of mild petroleum-heavy metal contaminated soil.

A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.

The invention discloses Sphingobacterium (sp.) SIT102 with the preservation number of CGMCC NO.6158 and a method for performing enantioselective hydrolysis to generate levetiracetam acid by utilizing the Sphingobacterium SIT102 serving as biocatalyst to catalyze racemization etiracetam acid esters. The method for preparing the levetiracetam acid comprises the steps of placing Sphingobacterium SIT102 cells into buffered solution, adding the racemization etiracetam acid esters into the buffered solution, performing the enantioselective hydrolysis through catalysis to obtain the levetiracetam acid. According to the method for preparing the levetiracetam acid by utilizing the Sphingobacterium SIT102 serving as the biocatalyst, the used biocatalyst is easy to prepare, the reaction condition is moderate, the yield of the levetiracetam acid can reach 48%, the enantiomer excess reaches 96%, and the production cost is low. Therefore, the method for preparing the levetiracetam acid by utilizing the Sphingobacterium has considerable industrial application development prospects.

The invention relates to a mixed microbial preparation for recovering soil polluted by composite pollutant oil in a biology manner and a manufacture method of the mixed microbial preparation. The mixed microbial preparation is made by mixing six bacterial strains including acinetobacter calcoaceticus, klebsiella pneumonia, enterobacter cancerogenus, pseudomonas stutzeri, rhodococcus erythropolis, and pseudomonas putida with one of rhodococcus, acinetobacter, gordonia, sphingobacterium, novosphingobium capsulatum, acinetobacter, and xanthomonas at a ratio of 1:1:2:2:1:1:1:1 and is used for recovering oil-polluted soil.

The invention belongs to the field of bioremediation of organic pollutants, and specifically discloses a Sphingobacterium sp. strain W144. The strain is preserved in China Center for Type Culture Collection, and has a preservation number of CCTCC NO:M2014151. The strain disclosed by the invention has rapid and efficient degradation ability on phenanthrene, biphenyl, pyrene, fluorene, fluoranthene and other organic pollutants, has a wide range of pH value, and salinity and temperature adaptation ability, and is a good material for bioremediation of polycyclic aromatic hydrocarbons in soil or the environment.

The invention provides a composite immobilized microbial agent as well as a preparation method and application thereof. The preparation method comprises the following steps of preparing a bacterial suspension of petroleum hydrocarbon degrading bacteria, wherein the petroleum hydrocarbon degrading bacteria comprise one or a combination of more of a pseudomonas pseudoalcaligenes strain, a pseudomonas guguanensis strain, am acinetobacter venetianus and a sphingobacterium pakistanense strain; adding biochar into the bacterial suspension, and uniformly mixing to enable the petroleum hydrocarbon degrading bacteria to be fully adsorbed on the biochar, so as to obtain a biochar-bacterial liquid mixed solution; and uniformly mixing a mixed NaCl aqueous solution of sodium alginate and polyvinyl alcohol with the biochar-bacterial liquid mixed solution to carry out cross-linking immobilization, dropwise adding the obtained mixed solution into a CaCl2-boric acid solution, cleaning the obtained mixed solution with the NaCl aqueous solution, placing the mixed solution on a sterile culture medium plate, and naturally airing. The method is simple and easy to implement, low in cost and high in operability; and the obtained composite immobilized microbial agent has excellent mechanical strength and excellent degradation effect on petroleum hydrocarbon.

The invention discloses a preparation method of a microbial agent for plant disease prevention and cure, and relates to the technical field of microbial prevention and cure of plant diseases. The steps are as below: (1) preparing the following raw materials: Paecilomyces lilacinus secondary bacterial fluid, Bacillus licheniformis-Sphingobacterium mixed fermentation fluid, Gram-positive actinomycetes-Bacillus cereus mixed fermentation fluid, nitrogen-fixing bacteria, Bacillus megaterium, photosynthetic bacteria, amino acids, humic acids, monosaccharides, chitin and modified diatomaceous earth;(2) preparing the Bacillus licheniformis-Sphingobacterium mixed fermentation fluid; (3) preparing the Gram-positive actinomycetes-Bacillus cereus mixed fermentation fluid; (4) preparing a mixed bacterial fluid A; and (5) adding the remaining raw materials to the mixed bacterial fluid A to obtain the microbial agent for plant disease prevention and cure. According to the present invention, the microbial agent contains a plurality of beneficial bacteria species, is effective for eliminating root-knot nematodes and eggs in soil by inhibiting bacteria with bacteria and by killing pests with bacteria, and is effective for inhibiting the growth and reproduction of soil-borne pathogenic bacteria.

The invention discloses Sphingobacterium multivorum and an application thereof. The Sphingobacterium multivorum is Sphingobacterium multivorum P6, the Sphingobacterium multivorum P6 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is May 31, 2021, and the preservation number is CCTCC NO: M2021648. The strain can convert insoluble inorganic phosphate into soluble phosphorus which can be directly absorbed and utilized by plants, the phosphorus solubilizing amounts of the strain cultured in four insoluble phosphate liquid culture media including calcium phosphate, iron phosphate, aluminum phosphate and ground phosphate rock for 7 days are 47.2 [mu]g / mL, 6.1 [mu]g / mL , 3.8 [mu]g / mL and 9.5 [mu]g / mL respectively, and a certain reference is provided for later research of wild strains. The Sphingobacterium multivorum has an important value for development of microbial fertilizers such as phosphate-solubilizing bacterial fertilizers and the like.

The invention discloses sphingobacterium multivorum and application thereof, and belongs to the technical field of bioengineering and environmental engineering. The sphingobacterium multivorum is collected in the China General Microbiological Culture Collection Center (CGMCC) on October 17, 2018 at the collection number of CGMCC No. 16596. The strain has heterotrophic nitrification and aerobic denitrification characteristics, can be applied to the field of nitrogen-containing sewage treatment and river treatment, has an ammonia nitrogen removal rate more than 90 percent, is pollution-free to the environment, achieves effects of quickly biologically removing nitrogen and recovering the self-cleaning capacity of a river, and has great significance for the field of environmental water treatment.

The invention relates to sphingobacterium sp., the classification name of the sphingobacterium sp. Is sphingobacterium sp., and the preservation number of the sphingobacterium sp. Is CGMCC (China General Microbiological Culture Collection Center) NO.18204. The sphingobacterium sp. The strain can be used for effectively degrading tetracycline antibiotics, and is of great significance for reducing environmental pollution and ecological imbalance caused by antibiotics.

The invention belongs to the biotechnological field and discloses a Sphingobacterium multivorum and a method for conversion of the Sphingobacterium multivorum into validamycin. The strain or thallus of the strain can convert the validamycin into valid amine and valid enamine; after being cultured in a seed culture medium, the strain or the thallus of the strain is inoculated into a transformation culture medium which contains substrate validamycin for decomposition of the substrate, or the thallus is obtained by separation after being cultured in the seed culture medium and the thallus obtained is added into validamycin solution for decomposition of the substrate; after separation and purification of decomposition liquor, the valid amine and the valid enamine are obtained. The invention also discloses a detection and separation method for the valid amine and the valid enamine. The strain Sphingobacterium multivorum is well-grown in the culture medium which contains the validamycin and the solution culture and transformation effect is good; moreover, the strain has the advantages of innocuity, no pathogenicity, stable performance, difficulty in contamination and so on.

The invention discloses a mixed bacterial flora for degrading fumonisin B1 and a crude enzyme preparation thereof. The mixed bacterial flora for degrading fumonisin B1 comprises the following components: Pseudomonas (Pseudomonas), Chaetomonas (Comamonas) and Sphingobacterium (sphingobacterium), wherein the total effective viable number of the mixed microflora accounts for more than 90% of the total number of the mixed microflora. The mixed bacterial flora degrading fumonisin B1 of the invention is amplified and cultured in MM culture medium containing sucrose, the bacterial body obtained by centrifugation is broken by ultrasonic wave, and the supernatant is lyophilized to obtain crude enzyme preparation. The mixed bacterial flora degrading fumonisin B1 of the invention is amplified and cultured in MM culture medium containing sucrose. The fumonisin B1 degradation rate of the mixed microflora and the crude enzyme preparation thereof obtained by the invention is above 90% in 24 hours. The mixed microbial flora and its crude enzyme preparation have the characteristics of simple fermentation and low production cost, and have broad application prospects for detoxification of fumonisin B1 in feedstuffs.

The invention relates to a preparation method for an amendment used for restoration of petroleum-heavy metal combinedly-contaminated saline soil. The preparation method comprises the following steps: airing carbon black to obtain a black powder or particle; preparing 8*10<10> / ml of a composite strain from bacillus subtilis and sphingobacterium multivolume in a ratio of 1: 1; air drying a biogas residue, crushing the biogas residue, sieving the crushed biogas residue with a 10-mesh sieve and compounding the biogas residue with carbon black, urea and a high efficiency petroleum degrading bacterium according to a certain proportion; and carrying out flat-mold type extrusion and granulation to produce a cylindrical particle, rounding the cylindrical particle by using a rounding machine, then screening the particle with a vibrating screen, allowing the particle to enter into a cooler for cooling and then carrying out weighing and bagging. The amendment prepared by using the method provided by the invention can reduce activity of heavy metals in the degradation process of petroleum while realizing high efficiency degradation of petroleum hydrocarbons in soil by using the petroleum degrading bacterium, accelerates growth of plants grown in saline soil and promotes micro-biological degradation efficiency by using the plants. The method provides a highly efficient, economic and safe amendment for microbe-plant restoration of mild petroleum-heavy metal contaminated soil.

The invention discloses a microorganism-containing composite soil remediation agent which comprises a composite microbial agent and composite powder, wherein the composite microbial agent comprises bacillus, sphingobacterium, pseudomonas and aspergillus oryzae, and the composite powder comprises diatomite, aquatic floaters, livestock and poultry manure, bagasse and corn straw. The composite powder is prepared from the following components in parts by weight: 20 to 40 parts of diatomite, 10 to 20 parts of aquatic floaters, 5 to 10 parts of livestock and poultry manure, 3 to 10 parts of bagasse and 20 to 30 parts of corn straws. Various production wastes obtained by agricultural production are adopted as raw materials, the manufacturing cost can be remarkably reduced, and due to the adoption of natural raw materials, secondary damage to soil is avoided, the remediation effect is excellent, and the soil remediation agent is more natural and environmentally friendly.

The invention belongs to the field of astragalus growth-promoting technology, and provides astragalus growth-promoting endophytic bacteria and its growth-promoting method and application in order to solve the current problems of astragalus membranaceus growth promotion and quality improvement. Promoting endophytes are: Sphingobacterium ( Sphingobacterium sp. ) strain KSCO1 and Pseudomonas phrysalis ( Pseudomonas poae ) strain KSCO2; on November 21, 2017, it was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation numbers are: CGMCC No.14945, CGMCC No.14946. 2~10μM E‑2 ‑Hexenal and 5-625 μM n-hexanol promoted the growth of KSC01, and 25-625 μM n-hexanol promoted the growth of KSCO2. A green, environment-friendly pesticide component with no medicinal material residue is provided for the cultivation of Chinese medicinal material Astragalus membranaceus.

The invention discloses sphingobacterium SC015 and application thereof in preparation of a norovirus adsorbent, the norovirus adsorption efficiency of the strain SC015 can reach 60%, and the strain SC015 has specificity for GII.4 subtype norovirus. Compared with the prior art (a filter membrane method), the strain SC015 has the advantages that the strain SC015 has special adsorption to norovirus, can be used to filter and adsorb specific subtypes of norovirus in drinking water or fresh-cut fruits and vegetables such as lettuce and strawberries, and can be used to effectively warn and remove pollution of the norovirus GII.4 on the surfaces of the fruits and the vegetables, the adsorption efficiency is improved by 30%, and the adsorption specificity is achieved (a traditional filter membrane method has no specificity).

The invention belongs to the field of bioremediation of organic pollutants, and specifically discloses a Sphingobacterium sp. strain W144. The strain is preserved in China Center for Type Culture Collection, and has a preservation number of CCTCC NO:M2014151. The strain disclosed by the invention has rapid and efficient degradation ability on phenanthrene, biphenyl, pyrene, fluorene, fluoranthene and other organic pollutants, has a wide range of pH value, and salinity and temperature adaptation ability, and is a good material for bioremediation of polycyclic aromatic hydrocarbons in soil or the environment.

The invention discloses the application of a strain of Sphingobacterium hotanense in preventing and treating parasitic nematode diseases. The Sphingobacterium hotanense AMCC 101218 provided by the present invention has a preservation number of CGMCC No.19217; this bacterium can effectively control cucumber root-knot nematode disease, and its oocyst number, root-knot number, and insect population density have decreased by 71.28% compared with the blank control , 75.86% and 63.80%; AMCC101218 genome size is 4490254bp, GC content is 41.21%.

The invention provides a livestock and poultry breeding sewage treatment agent and application thereof. The livestock and poultry breeding sewage treatment agent is prepared by mixing microbial flora,mineral filler, magnesium salt, phosphate, organic fertilizer and humus, and the microbial flora is prepared from sphingobacterium S-4, bacillus gibsonii S-2, rhodococcus R-03, rhodobacter sphaeroides R-06 and paenibacillus polymyxa EBL-06. The livestock and poultry breeding sewage treatment agent is used for treating livestock and poultry breeding sewage to obtain a nutrient solution capable ofbeing reutilized. While livestock and poultry breeding sewage is treated, original sewage has biological activity, and stabilization, harmlessness and high-added-value recycling of the livestock and poultry breeding sewage are achieved. The obtained nutrient solution meets the safety index, the effectiveness index and the requirements of part of typical plant pathogenic microorganism antagonism experiments, can be widely applied to plant cultivation and crop planting, enables plants to be more luxuriant, green and robust, facilitates sustainable utilization of soil, and enables the soil to besoft and good in air permeability.

The invention provides a compound biological enzyme preparation, a compound microbial agent and application thereof in the treatment of oily sludge. The compound biological enzyme preparation is prepared from bacillus subtilis, bacillus pumilus, candida and lactobacillus brevis. The compound microbial agent is prepared from candida tropicalis, sphingobacterium multivolume, bacillus cereus, acinetobacter calcoaceticus and bacillus megaterium. The invention also provides the application of the compound biological enzyme preparation and / or the compound microbial agent in the treatment of oily sludge and a method for treating the oily sludge. The method comprises steps of treating the oily sludge with the compound biological enzyme preparation to form an upper oil phase, an intermediate aqueous phase and a lower sludge phase, and treating the lower sludge phase and / or the intermediate aqueous phase by using the composite microbial agent. The compound biological enzyme preparation, the compound microbial agent and the application thereof provided by the invention have the advantages of harmless treatment of oily sludge, low cost, simplicity and convenience, no secondary pollution, and broad application prospects.

The invention discloses a compound microorganism bacterium agent for degrading petroleum hydrocarbon as well as a preparation method and application of the compound microorganism bacterium agent. The compound microorganism bacterium agent is prepared from active acinetobacter KJ-1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.20664, Providencia reevei L1 with the preservation number of CGMCC No.16526, bacillus SWH-1 with the preservation number of GMCC No.1950 and sphingobacterium SWH-2 with the preservation number of CGMCC No.1951. The petroleum hydrocarbon degradation composite microbial agent prepared by the invention can effectively degrade petroleum hydrocarbon substances in soil and oily sludge sand, can be effectively applied to biodegradation treatment of petroleum-polluted soil, and does not cause secondary pollution to the ecological environment of the soil; the synergistic effect of the acinetobacter live KJ-1, the province reevesii L1, the bacillus SWH-1 and the sphingobacterium SWH-2 is fully utilized, the final degradation efficiency of the petroleum hydrocarbon is improved, the preparation process of the solid microbial inoculum is simple and easy to operate, the cost is low, and the solid microbial inoculum is suitable for industrial production. The method has a wide application prospect in the field of bioremediation of petroleum-contaminated soil and petroleum product leakage accident sites.