39 results about "Sphingobacterium" patented technology

DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.

A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.

A process for preparing heparin oligose from heparinase includes such steps as culturing sphingobacterium (CGMCC No.0660), preparing non-cell coarse enzyme liquid, extracting and purifying heparinase, degradating heparin by the heparinase at 20-30 deg.C to obtain heparin oligose mixture, ultrafilter, gel filter for fractionation and evaporation concentration. The product has the activity of resisting smooth muscle hyperplasia.

The invention relates to a preparation method for an amendment used for restoration of petroleum-heavy metal combinedly-contaminated saline soil. The preparation method comprises the following steps: airing carbon black to obtain a black powder or particle; preparing 8*10<10> / ml of a composite strain from bacillus subtilis and sphingobacterium multivolume in a ratio of 1: 1; air drying a biogas residue, crushing the biogas residue, sieving the crushed biogas residue with a 10-mesh sieve and compounding the biogas residue with carbon black, urea and a high efficiency petroleum degrading bacterium according to a certain proportion; and carrying out flat-mold type extrusion and granulation to produce a cylindrical particle, rounding the cylindrical particle by using a rounding machine, then screening the particle with a vibrating screen, allowing the particle to enter into a cooler for cooling and then carrying out weighing and bagging. The amendment prepared by using the method provided by the invention can reduce activity of heavy metals in the degradation process of petroleum while realizing high efficiency degradation of petroleum hydrocarbons in soil by using the petroleum degrading bacterium, accelerates growth of plants grown in saline soil and promotes micro-biological degradation efficiency by using the plants. The method provides a highly efficient, economic and safe amendment for microbe-plant restoration of mild petroleum-heavy metal contaminated soil.

The invention relates to the field of sewage treatment, and specifically discloses an aerobic denitrification bacterial agent, including Klebsiella pneumoniae bacterial liquid, Raoultella vegetative bacterial liquid, Bacillus flexus bacterial liquid, Sphingobacterium bacterial liquid, Gram Lebsiella bacteria liquid, Acinetobacter baumannii bacteria liquid and Pseudomonas steerii bacteria liquid. A method for preparing an aerobic denitrifying bacterial agent, comprising the following steps: step a: activation of strains; step b: expanded cultivation; step c: preparing the bacterial agent, mixing the bacterial liquid in step b to prepare a mixed bacterial liquid, Inoculate the mixed bacterial solution into the aerobic denitrification medium and culture it with constant temperature shaking for 24h~48h. When the total number of microorganisms is greater than 10 9 When the CFU / ml is used, the bacterial agent is collected and divided into sterile containers for storage. The technical solution solves the problems of high cost and difficult management in the sewage treatment process in the prior art.

A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.