Sphingobacterium SC015 and application thereof in preparation of norovirus adsorbent

A technology of sphingosine bacteria and viruses, applied in the field of norovirus adsorption and removal, can solve problems affecting economic development and social stability, and achieve the effect of improving adsorption efficiency

Pending Publication Date: 2021-12-31
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If there is no timely and effective prevention and control measures,

Method used

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  • Sphingobacterium SC015 and application thereof in preparation of norovirus adsorbent
  • Sphingobacterium SC015 and application thereof in preparation of norovirus adsorbent
  • Sphingobacterium SC015 and application thereof in preparation of norovirus adsorbent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Screening of norovirus-adsorbed bacteria on the surface of lettuce

[0035] Before separating and screening norovirus-binding bacteria in the lettuce sample, the bacteria screening container and the like were sterilized (high temperature and high pressure sterilization treatment).

[0036] 1. Pretreatment of lettuce samples

[0037] Rinse lettuce (var.ramosa Hort.) leaf surface and leaf sheath with distilled water, use sterilized scissors to randomly take lettuce leaves and leaf sheath 5g into a conical flask, then add 50mL of PBS buffer solution, use glass rod to stir for 5min, double Filter the bacterial suspension with a layer of gauze, centrifuge at 5000rpm for 5min, discard the supernatant, resuspend the pellet with 2ml of PBS, centrifuge at 1000rpm for 1min, and collect the supernatant.

[0038] 2. Screening of bacteria expressing HBGAs

[0039] Add 50 μL of three anti-human tissue serum antigen A, B, (O)H mouse monoclonal antibody dilutions prepared ...

Embodiment 2

[0054] Example 2: Biological Identification of Strain SC015

[0055] 1. Morphological characteristics of the strain

[0056] (1) Morphological characteristics of colony population: Streak inoculation of monoclonal colony of strain SC015 onto TSA solid medium, place the plate upside down in a constant temperature incubator, and incubate at 27°C for 4-5 days. The colony is round, milky yellow and opaque, with a smooth and moist surface , slightly convex in the center, regular edges, no halo, diameter 3-5mm, see Figure 4 .

[0057] (2) Individual morphological characteristics of the bacteria: Gram staining was carried out on the bacterial strain SC015, and observation by an optical microscope showed that the bacterial strain was a rod-shaped, Gram-negative bacterium.

[0058] 2. Strain 16Sr RNA analysis

[0059]Bacterial Genomic DNA Extraction Kit (QIAGEN) was used to extract the genomic DNA of strain SC015. Using the DNA as a template, the 16S rRNA fragment of the bacterial ...

Embodiment 3

[0068] Embodiment 3: Sphingobacterium SC015 bacterial liquid

[0069] 1. Sphingobacterium SC015 culture: Sphingobacterium SC015 was inoculated into TSA solid medium, placed in a constant temperature incubator at 25°C, and cultured statically for 4-5 days. The bacteria are picked and inoculated into TSB liquid medium, cultured at 25° C. and 220 rpm on a shaker for 4-5 days to obtain seed liquid.

[0070] 2. Preservation of Sphingobacterium SC015 strains: put 1mL of 40% glycerin into a glycerol tube for high-pressure steam sterilization, take 1mL seed liquid and put it into a glycerin tube with a pipette gun and mix well to make the glycerin concentration reach 20% , marked and pre-cooled at 4°C for 30 minutes, and then stored in a -80°C refrigerator.

[0071] 3. Fermentation culture of Sphingobacterium SC015: inoculate the seed solution of Sphingobacterium SC015 with an inoculum volume concentration of 5% into a Erlenmeyer flask containing a fermentation medium, and cultivate ...

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Abstract

The invention discloses sphingobacterium SC015 and application thereof in preparation of a norovirus adsorbent, the norovirus adsorption efficiency of the strain SC015 can reach 60%, and the strain SC015 has specificity for GII.4 subtype norovirus. Compared with the prior art (a filter membrane method), the strain SC015 has the advantages that the strain SC015 has special adsorption to norovirus, can be used to filter and adsorb specific subtypes of norovirus in drinking water or fresh-cut fruits and vegetables such as lettuce and strawberries, and can be used to effectively warn and remove pollution of the norovirus GII.4 on the surfaces of the fruits and the vegetables, the adsorption efficiency is improved by 30%, and the adsorption specificity is achieved (a traditional filter membrane method has no specificity).

Description

technical field [0001] The invention relates to a Norovirus adsorption and removal technology, in particular to a strain of Sphingobacterium sp. SC015 and its application in adsorption of Norovirus. Background technique [0002] Norovirus (NoV) can infect humans and cause acute gastroenteritis. It was named after it was isolated and discovered in a diarrhea outbreak in Norwalk, Ohio, USA in 1968, and was first detected by Kapikian in 1972. NoV has been recognized as the most common cause of epidemic or sporadic gastroenteritis in children and adults, both in developing and developed countries. NoV is a non-enveloped single-stranded positive-sense RNA virus belonging to the family Caliciviridae, which can be divided into five gene groups (Genogroup) from GI to GV, among which the GI and GII groups mainly cause human infection. Through mutation and recombination, the genome of norovirus undergoes rapid mutation, showing gene diversification, which leads to the generation of n...

Claims

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Application Information

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IPC IPC(8): A23L5/20C02F3/34
CPCA23L5/273A23L5/28C02F3/34Y02A50/30
Inventor 廖宁波
Owner JIANGXI AGRICULTURAL UNIVERSITY
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