Sphingobacterium and method for preparing levetiracetam acid by utilizing same

Abstract

The invention discloses Sphingobacterium (sp.) SIT102 with the preservation number of CGMCC NO.6158 and a method for performing enantioselective hydrolysis to generate levetiracetam acid by utilizing the Sphingobacterium SIT102 serving as biocatalyst to catalyze racemization etiracetam acid esters. The method for preparing the levetiracetam acid comprises the steps of placing Sphingobacterium SIT102 cells into buffered solution, adding the racemization etiracetam acid esters into the buffered solution, performing the enantioselective hydrolysis through catalysis to obtain the levetiracetam acid. According to the method for preparing the levetiracetam acid by utilizing the Sphingobacterium SIT102 serving as the biocatalyst, the used biocatalyst is easy to prepare, the reaction condition is moderate, the yield of the levetiracetam acid can reach 48%, the enantiomer excess reaches 96%, and the production cost is low. Therefore, the method for preparing the levetiracetam acid by utilizing the Sphingobacterium has considerable industrial application development prospects.

Description

technical field [0001] The invention relates to a bacterium and its application, in particular to a sphingobacterium and a method for preparing levetiracetam acid by using the sphingobacterium as a biocatalyst. Background technique [0002] Levetiracetam acid is known to be synthesized from levetiracetam (( S )-α-Ethyl-2-oxo-1-acetamide pyrrolidine, the key chiral intermediate of Levetiracetam), whose structural formula is as figure 1 shown. [0003] Levetiracetam is a new type of antiepileptic drug developed by UCB (UCB) in Belgium. It was approved by the FDA in April 2000 and marketed in the United States and the European Union. It is mainly used for the treatment of localized and secondary systemic epilepsy. sexual epilepsy. It is currently on the market in more than 66 countries and regions around the world, and has more than 1 million treatment records worldwide. It is currently the most widely used new antiepileptic drug in the treatment of epilepsy in the United S...

AI Technical Summary

Problems solved by technology

[0007] One of the purposes of the present invention is to solve technical problems such as the use of a large amount of organic solvents, cumber...

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