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Sphingobacter strains for degrading polycyclic aromatic hydrocarbon organic pollutants and their applications

A technology of organic pollutants and sphingosine bacteria, which is applied in the field of environmental organic pollutants remediation and treatment, can solve problems such as threatening human health, accumulation of PAHs, damage to soil ecological environment, etc., and achieve the effect of high efficiency and rapid degradation ability.

Active Publication Date: 2017-06-20
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, PAHs pollution in soil will destroy the soil ecological environment, affect soil productivity, and reduce crop yield; at the same time, it will lead to the accumulation of PAHs in agricultural products, which directly threatens human health

Method used

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  • Sphingobacter strains for degrading polycyclic aromatic hydrocarbon organic pollutants and their applications
  • Sphingobacter strains for degrading polycyclic aromatic hydrocarbon organic pollutants and their applications
  • Sphingobacter strains for degrading polycyclic aromatic hydrocarbon organic pollutants and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation and identification of W144 bacterial strain

[0021] The long-term oil-contaminated soil from Qianjiang Jianghan Oilfield in Hubei was used as the screening soil. The specific screening scheme is as follows:

[0022] 1) Take 10g of soil, add it to the inorganic salt medium MSM without carbon source, and then add Phenanthrene to make the final concentration 100mg / L; culture with shaking at 28°C for 5 days, and then inoculate with 5% into the corresponding fresh medium; transfer 5 times in succession;

[0023] 2) Take 0.1ml of the enriched culture solution, spread it on the MSM solid plate containing phenanthrene, and after static culture at 28°C for 5 days, transfer the colonies grown on the plate to the plate containing phenanthrene, and continue to grow; then Select fast-growing colonies for plate streak purification;

[0024] 3) Pick stable growing colonies, inoculate them into MSM medium containing 100 mg / L phenanthrene, and cultivate them with ...

Embodiment 2

[0028] Example 2 Degradation characteristics of polycyclic aromatic hydrocarbons by strain W144

[0029] The inventors studied the ability of the W144 strain to degrade common polycyclic aromatic hydrocarbons. Based on the MSM medium without carbon source, add different polycyclic aromatic hydrocarbons as the sole carbon source, so that the final concentration is 100 mg / L; then inoculate W144 culture in logarithmic growth phase with 1% inoculum , placed at 28°C for 5 days with shaking culture, measured the OD of the culture 600 absorbance value. The medium without any carbon source was used as negative control. Since no carbon source other than polycyclic aromatic hydrocarbons was added to the medium, the growth rate and biomass of the strain reflected the ability of the strain to utilize and degrade polycyclic aromatic hydrocarbons.

[0030] like figure 2 As shown, W144 has the maximum utilization ability for phenanthrene, and has strong degradation ability for biphenyl,...

Embodiment 3

[0031] Example 3 Degradation characteristics of phenanthrene by W144 strain

[0032] In order to further analyze the degradation characteristics of the W144 strain provided by the present invention to polycyclic aromatic hydrocarbons, the inventor tested the growth rate and degradation rate of the W144 strain with phenanthrene as the sole carbon source. The W144 strain in the logarithmic growth phase was inoculated with 1% inoculum into the MSM medium with 500 mg / L phenanthrene as the sole carbon source, and cultured with shaking at 28°C. Meanwhile, measure the OD of the culture every 12 hours 600 value and concentration of residual phenanthrene.

[0033] like image 3It was shown that W144 bacteria had the ability to degrade phenanthrene from the beginning of inoculation, and the degradation ability and strain growth increased rapidly after 12 hours, and, as the residual phenanthrene concentration in the culture decreased, the growth of the strain gradually entered a platea...

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Abstract

The invention belongs to the field of bioremediation of organic pollutants, and specifically discloses a Sphingobacterium sp. strain W144. The strain is preserved in China Center for Type Culture Collection, and has a preservation number of CCTCC NO:M2014151. The strain disclosed by the invention has rapid and efficient degradation ability on phenanthrene, biphenyl, pyrene, fluorene, fluoranthene and other organic pollutants, has a wide range of pH value, and salinity and temperature adaptation ability, and is a good material for bioremediation of polycyclic aromatic hydrocarbons in soil or the environment.

Description

technical field [0001] The invention belongs to the field of restoration and treatment of environmental organic pollutants. Specifically, it relates to a strain of Sphingobacterium for degrading polycyclic aromatic hydrocarbon organic pollutants and its application. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of organic compounds containing two or more benzene ring structures. Stable, and become typical persistent organic pollutants. PAHs have "carcinogenic, teratogenic, and mutagenic" effects, and have great potential harm to environmental ecological safety and human health. Since the 1980s, environmental protection bureaus around the world have listed PAHs as priority monitoring pollutants in the environment. PAHs in the environment mainly come from petroleum production and transportation, petroleum refining, organic industry, fossil energy combustion, vehicle exhaust emissions, etc.; natural volcan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10A62D3/02C12R1/01A62D101/26
Inventor 陈雯莉黄巧云汪方奎李偲王少一
Owner HUAZHONG AGRI UNIV
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