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Sphingobacterium oryzae and application thereof

A technology of Sphingobacterium and Mizutani, applied in the field of microorganisms, can solve problems that affect the health and safety of the soil ecological environment, limited degradation genes, and affect the green and sustainable development of agriculture.

Pending Publication Date: 2022-06-24
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Residues of tetracycline antibiotics have been detected in a variety of environments, but the currently obtained tetracycline antibiotic-related degradation strains and degradation genes are very limited, especially the research on the further degradation and residue of intermediate metabolites is still blank , the problem of phytotoxicity caused by the residues of tetracycline antibiotics has seriously affected the health and safety of the soil ecological environment, and seriously affected the green and sustainable development of agriculture.

Method used

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  • Sphingobacterium oryzae and application thereof
  • Sphingobacterium oryzae and application thereof
  • Sphingobacterium oryzae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] This example is used to illustrate the isolation and identification of strain S121

[0044] Tetracycline: Tetracycline hydrochloride (C 22 H 24 N 2 O 8 HCl), purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. CAS: 64-75-5. Oxytetracycline: oxytetracycline hydrochloride (C 22 H 24 N 2 O 9HCl), purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. CAS: 2058-46-0.

[0045] Take 1 g of the sample (the sample comes from the contaminated soil of a pharmaceutical factory in Hebei) and inoculate it into 100 mL of a carbon-free inorganic salt medium (NH4) containing 50 mg / L tetracycline. 4 Cl 1.1g / L, Na 2 HPO 4 ·12H 2 O 1.0g / L, KH 2 PO 4 0.5g / L, MgSO 4 ·7H 2 00.2g / L, pH 7.0) for enrichment culture, diluted to 10 7 Coat on 20mL of LB liquid medium containing 50mg / L tetracycline (tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L, pH 7.0), isolate and obtain multiple strains of bacteria that can tolerate tetracycline, One of the strains was i...

Embodiment 2

[0048] This example is used to illustrate that Sphingosine glutamicum S121 can degrade tetracycline antibiotics.

[0049] Sphingosine glutamicum S121 was inoculated into 100 mL of LB liquid selective medium containing 50 mg / L tetracycline (tryptone 10 g / L, yeast extract 5 g / L, NaCl 10 g / L, pH 7.0), placed at 30 ° C 150r / min constant temperature shaker for 7d, sampling every 24h, using high performance liquid chromatography (HPLC) to determine the residual amount of tetracycline in the culture solution under different incubation times, and take 5, 10, 20, 50, 100, 200 mg / L concentration of tetracycline to draw a standard curve (CK) of tetracycline.

[0050] The same method was used to inoculate Sphingosine glutamicum S121 into the above-mentioned LB liquid selective medium containing 50 mg / L oxytetracycline, and the residual amount of oxytetracycline in the culture solution at different incubation times was determined.

[0051] The HPLC determination method refers to the nati...

Embodiment 3

[0055] This example is used to optimize the degradation conditions for the degradation of tetracycline antibiotics by Sphingosine glutamicum S121.

[0056] Using the method described in Example 3, under the same conditions of other culture parameters, Sphingosine glutamicum S121 has the highest degradation rate of tetracycline in the LB medium containing 5-20 mg / L tetracycline;

[0057] Under the same conditions of other culture parameters, in the LB medium with 20mg / L tetracycline, strain S121 had the highest degradation rate of tetracycline at 30-35℃, and could achieve complete degradation after 5 days;

[0058] Under the same conditions of other culture parameters, the strains at pH 7-10 completely degraded tetracycline after 5 days, and the degradation rate of tetracycline at pH 6 was 87%;

[0059] Under the same conditions of other culture parameters, the inoculation amount of the strain in the range of 0.5-5% (v / v) has no significant effect on the degradation rate of tet...

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Abstract

The invention relates to sphingobacterium sp., the classification name of the sphingobacterium sp. Is sphingobacterium sp., and the preservation number of the sphingobacterium sp. Is CGMCC (China General Microbiological Culture Collection Center) NO.18204. The sphingobacterium sp. The strain can be used for effectively degrading tetracycline antibiotics, and is of great significance for reducing environmental pollution and ecological imbalance caused by antibiotics.

Description

technical field [0001] The disclosure relates to the technical field of microorganisms, in particular, to a Sphingobacterium glutaeus, a microbial agent and its application in degrading tetracycline antibiotics. Background technique [0002] Antibiotics refer to a class of secondary metabolites with anti-pathogen or other activities produced by microorganisms (including bacteria, fungi, actinomycetes) or higher animals and plants in the course of life, which can interfere with the developmental functions of other living cells Chemical material. Among them, tetracycline antibiotics are broad-spectrum antibiotics that are widely used in agriculture, animal husbandry and aquaculture. They have a stable structure, can exist in the environment for a long time, and cannot be completely metabolized by humans and animals. Residues of tetracycline antibiotics have been detected in a variety of environments, but the currently obtained tetracycline antibiotic-related degradation strai...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/22A62D101/26A62D101/28
CPCC12N1/20A62D3/02A62D2101/22A62D2101/26A62D2101/28
Inventor 阮志勇孔德龙谭浩马青云周义清江旭
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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