Mixed microbial preparation and manufacture method thereof

A technology of mixed microorganisms and manufacturing methods, which is applied in the field of mixed microbial preparations and its manufacturing, and can solve problems such as difficult effects, difficult recovery of oil-contaminated soil, and difficult effective treatment of a wide range of contaminated areas.

Inactive Publication Date: 2017-05-17
安徽壹诺环境工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above-mentioned existing microbial preparations and their manufacturing methods have limitations in minimizing the impact on the ecological environment in the process of using microbial preparations to restore soil contaminated by oils of complex pollutants. In particular, they have limitations in Limitations in the biological restoration of soils contaminated with oils with a wide range of molecular weights and a variety of molecular structures containing linear hydrocarbons or complex pollutants of aliphatic and aromatic hydrocarbons
[0007] Existing microbial preparations and their manufacturing methods also fail to consider the charact

Method used

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  • Mixed microbial preparation and manufacture method thereof
  • Mixed microbial preparation and manufacture method thereof
  • Mixed microbial preparation and manufacture method thereof

Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0041] Experimental Example 1: Manufacture of a mixed microbial preparation with an oil-decomposing ability to achieve an optimal microbial composition ratio

[0042] First, the measurement of the decomposition rate using a biochemical oxygen demand (BOD, biochemical oxygen demand) intelligent coagulation test mixer (jar tester) will be described below.

[0043] figure 1 It is a figure explaining the manufacturing method of the mixed microorganism preparation of the Example of this invention.

[0044] refer to figure 1 , Accurately measured 160 soils where the dry soil was polluted by diesel oil (Diesel) at 10000ppm (mg / kg). After diluting the above-mentioned microorganisms and adding the moisture to 20% (step S101), use tweezers to put NaOH (Sodium hydroxide) in the rubber bag of the tablets to make the biochemical oxygen demand intelligent coagulation test mixer (OxiTOP) was completely sealed, and placed in a culture medium at 20° C. for zero calibration, thereby measurin...

no. 2 example

[0052] Example 2: Production of a mixed preparation of oil-decomposing bacterial strains with excellent soil permeability

[0053] First, a description of the permeability experiments follows.

[0054] Figure 4 It is a diagram showing microcolumns used in soil permeability experiments of microorganisms in Examples of the present invention.

[0055] As mentioned above, the dried soil was re-screened using a soil volume of 40-70 mesh, and in order to carry out the soil permeability experiment on the above-mentioned microorganisms, a plastic syringe with a small volume (for example, 3 mL) was used as Figure 4 After peeling off the filter paper (Whatman No.4), inject 1.5g of the screened soil into a 40-70mesh soil body. At this time, after the soil poured into each microcolumn was uniformly filled, the volume was kept constant.

[0056] In order to remove the influence of free-standing bacteria in the soil, after 20 minutes of wet sterilization at 121°C, put it in a dry box (...

no. 3 example

[0072] The third experimental example: Soil improvement method of oil-decomposing bacteria strain

[0073] First, osmotic regulation based on chemical changes in the cell wall of microorganisms is explained as follows.

[0074] After performing the above-mentioned step S104, as microorganisms judged to be good in oil decomposing property and used for soil permeability, Acinetobacter calcoaceticus, Klebsiella pneumoniae, Enterobacter carcinogens In the osmotic method, methyl methanesulfonate (methyl methanesulfonate; MMS, Sigma) was used to perform cell wall composition changes of the above-mentioned microorganisms (step S105 ). Furthermore, after each microorganism was cultured overnight (for example, 16 hours to 18 hours) in the M9 minimal medium, it was re-inoculated in the state of OD6000.05~0.1 in the minimal medium, and cultured to OD6000.6~0.8 ( exponential phase). The microorganisms cultivated to the index were centrifuged (8000rpm, 10min) to remove the supernatant, a...

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Abstract

The invention relates to a mixed microbial preparation for recovering soil polluted by composite pollutant oil in a biology manner and a manufacture method of the mixed microbial preparation. The mixed microbial preparation is made by mixing six bacterial strains including acinetobacter calcoaceticus, klebsiella pneumonia, enterobacter cancerogenus, pseudomonas stutzeri, rhodococcus erythropolis, and pseudomonas putida with one of rhodococcus, acinetobacter, gordonia, sphingobacterium, novosphingobium capsulatum, acinetobacter, and xanthomonas at a ratio of 1:1:2:2:1:1:1:1 and is used for recovering oil-polluted soil.

Description

technical field [0001] The technical field of the present invention relates to a mixed microbial preparation and a manufacturing method thereof, in particular to a mixed microbial preparation and a manufacturing method thereof for biologically restoring soil contaminated by oils of complex pollutants. Background technique [0002] Since the oil-contaminated soil is not polluted by one type of oil, but by multiple oils in a complex manner, only the combined use can have high-efficiency and selective decomposition of each oil The ability of microbial agents can be effectively decontaminated. [0003] Among the microbial agents known so far, although many kinds of microbial agents have oil decomposition ability, they either have weak decomposition ability according to the characteristics of each microbial agent, or show selective decomposition ability according to the type of oil, In addition, in a high-concentration oil-contaminated area, many problems such as weakening of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01C12R1/22C12R1/38C12R1/40C12R1/20C12R1/64
Inventor 张国良
Owner 安徽壹诺环境工程有限公司
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