103 results about "Rhodococcus erythropolis" patented technology

The invention discloses a solid microbial agent used for remedying the soil contaminated by petroleum, belonging to the technical field of bioremediation of soil contaminants. First-class seed liquid of acinetobacter radioresistens and rhodococcus erythropolis are mixed in equal parts after two steps of fermentation in liquid and solid states and cultivation to obtain the solid microbial agent, wherein, the number of effective viable bacteria of acinetobacter radioresistens and rhodococcus erythropolis is 10 to 10 CFU/g. The solid microbial agent in the invention has the advantages of low cost, high number of viable bacteria; the invention further has the advantages that micro-ecological environment improvement of the soil can be improved and secondary contamination cannot be caused to the environment, thus applicable to the bioremediation of the soil which is contaminated by petroleum and petroleum products.

Genes have been isolated from Rhodococcus erythropolis AN12 strain encoding the isoprenoid biosynthetic pathway. The genes and gene products are the first isolated from a Rhodococcus strain. The genes and gene products of the present invention may be used in a variety of ways for the production of isoprenoid compounds in a variety of organisms.

The invented Rhodococcus erythropolis LSSE8-1 is preserved in "general Microbe Centre, Chinese Microbe Culture Preservation & Management Committee" in 9,10,2001, its preservation number is CGMCCNO.0643; its Gram reaction is positive, partial acidoresistant and aerobic; after 14 hr. of sheet-inserting culture at 30 deg.C on the glucose asparagine agar and nutrient agar, its hypha is started breaking from centre of colony, after 24 hr. the globular or rhabdoid corpuscle can be produced, after 72 hr. the circumference of colony can be continuously grown, there is not aerial mycelium, occasionally it has ramification, it has no requirement for vitamin B1, it contains meso diaminopimelic acid, and has characteristic arabinose and galactose, its cell wall belongs to IV type.

The invention provides a method for preparing and synthesizing a statin medicinal intermediate, namely, an (R)-3-hydroxy-glutaric acid ethyl ester or a hydroxy substituted derivative of the (R)-3-hydroxy-glutaric acid ethyl ester with high optical activity by performing chiral separation on a racemic 4-cyano-group-3-hydroxy butyric acid ethyl ester or a hydroxy substituted derivative of the 4-cyano-group-3-hydroxy butyric acid ethyl ester and a strain. The method comprises the following steps: performing a conversion reaction in a reaction system in which a compound shown in a formula (11) is used as a substrate and an enzyme obtained by fermenting and culturing rhodococcus erythropolis CCTCC No:M 209244 or CCTCC No:M 209194 is used as a catalyst at the temperature of between 10 and 50 DEG C; and after the reaction, separating and purifying reaction liquid to obtain the compound shown in formula (I). The method realizes obtaining the (R)-3-hydroxy-glutaric acid ethyl ester or the hydroxy substituted derivative of the (R)-3-hydroxy-glutaric acid ethyl ester with high optical activity by catalyzing and hydrolyzing the racemic 4-cyano-group-3-hydroxy butyric acid ethyl ester or the hydroxy substituted derivative of the 4-cyano-group-3-hydroxy butyric acid ethyl ester in one step under the condition of not hydrolyzing an ester group in the substrate or producing any byproduct, and overcomes the defects of more byproducts, low target product yield, low optical purity and the like brought by a conventional synthetic method in a hydrolyzing process, such as a chemical method.

The invention aims to provide a production method for a compound microbial preparation for aquatic products. The production method comprises the following steps: respectively carrying out high-density liquid fermentation on strains of bacillus cereus, bacillus licheniformis, halophilic pseudomonas, lactobacillus acidophilus, rhodococcus erythropolis, rhodopseudomonas palustris and enterococcus faecium to obtain ultra-concentrated bacterium powder; and then preparing according to a ratio to obtain the compound microbial preparation. The production process is simple in process, is applicable to most existing fermentation processes and equipment and is good for being popularized and applied in a large scale.

The invention discloses a mariculture water body purification composite microbial preparation, and belongs to the technical field of water body purification. The contents of component concentrated bacterial powders of the composite microbial preparation comprise 20-50% of bacillus subtilis, 5-15% of bacillus licheniformis, 10-30% of bacillus cereus, 1-5% of pseudomonas aeruginosa, 1-5% of rhodococcus erythropolis, 5-10% of pseudomonashalophila, 5%-15% of rhodopseudomonas palustris, and 15%-40% of enterococcus faecium. After the bacterial powders are mixed in proportion, packaging is carried out by adopting a water soluble material. The composite microbial preparation is put into a mariculture water body, enables dominant strains to be reproduced rapidly, allows culture pond redundant remnant feeds, feces, animal and plant dead bodies and other organic pollutants to be decomposed by active and abundant enzyme systems, and can significantly reduce the contents of ammonia nitrogen and nitrite in the culture water body.

The invention provides a compound microorganism preparation. The compound microorganism preparation is composed of Bacillus cereus, bacillus licheniformis, halophilic pseudomonades, Lactobacillus acidophilus, Rhodococcus erythropolis, Rhodopseudomonas palustris and enterococcus faecium. The viable count of the compound microorganism preparation is as high as 8*1011CFU/g, and the compound microorganism preparation can be widely applied to the aquaculture water areas of fresh water and seawater. Ammonia nitrogen and nitrite in the water can be effectively degraded and water blooms can be restrained under the synergistic effect of the bacterial strains, therefore, the aquatic water can be purified, growing environment for breeding animals can be improved, and productivity can be improved.

The invention belongs to the technical field of aquaculture and discloses a compounded oxygen producer for removing nitrite in aquatic water and a preparation method. The oxygen producer is prepared from components in parts by weight as follows: 40-50 parts of oxygen production ingredients, 24-38 parts of a compound microbial agent, 5-29 parts of an adsorbent, 1.0-2.5 parts of a growth accelerant, 7-10 parts of a traditional Chinese medicine admixture, 0.02-0.12 parts of a synergistic agent, 1-3 parts of a stabilizer and 1.4-2.2 parts of a sustained-release agent; the compound microbial agent comprises denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, bacillus laterosporus, bacillus subtilis, rhodococcus erythropolis, silicate bacteria, streptococcus faecalis and bacillus coagulans. Multiple microbial agents are compounded in the oxygen producer, and the compounded oxygen producer can effectively remove nitrite, has the functions of inhibiting pathogenic bacteria in the aquatic water and improving digestion of intestinal tracts of aquatic animals and has better popularization and application value.

The invention relates to a salt-tolerant aerobic denitrification bacterium rhodococcus erythropolis LH-N13 with a collection number of CGMCC No. 6974. The invention also discloses a microbial agent of rhodococcus erythropolis and an application of the microbial agent in high-salinity nitrogen-containing wastewater. The strain is screened from salt field sludge and has strong tolerance against high salinity; under the condition of the salinity (based on NaCl) less than or equal to 15%, nitrate nitrogen and nitrite nitrogen in the sewage can be removed, the microbial agent prepared from the strain can be directly used for denitrifying high-salinity wastewater, can be used for solving the difficult problem that the biological nitrogen removal total nitrogen is difficult to meet the standard under a high-salinity environment and has good application prospects. The salt-tolerant denitrification bacterium rhodococcus erythropolis LH-N13 or the microbial agent prepared from rhodococcus erythropolis LH-N13 can be used for the biological treatment of the high-salinity nitrogen-containing wastewater produced in food processing, bio-brewing, pesticides and leather.

The invention discloses a Rhodococcus erythropolis preserved in CRCCC in Wuhan University in 6,20,1005 with number of M205068. It also discloses its application in crude oil desulfurization that comprises: (1) slant cultivation; (2) seed cultivation; (3) enlarge cultivation; (4) collecting thalli; (5) preparing diapause cell; (6) treating crude oil sample; (7) sample detection. This invention has high desulfurization ratio with simple operation and bright application future.

The invention discloses a preparation method of a microbial flocculant complex and an application of the microbial flocculant complex in water treatment. The microbial flocculant complex is prepared by compounding a microbial flocculant mixture with a natural high-molecular modified flocculant. The microbial flocculant mixture is produced by fermentation with rhodococcus erythropolis ATCC 10543, bacillus licheniformis CGMCC 1.6510, streptomyces parvus CGMCC 4.5927, cryytococcus neoformans CGMCC 2.1320 and syncephalastrum racemosum CGMCC 3.3737; the natural high-molecular modified flocculant isprepared by modification with flaxseed gum as a matrix, H2O2-FeSO4 as an initiator and a triethylamine and epoxy chloropropane copolymer as a cationic monomer. When the microbial flocculant complex is used in water treatment, the flocculation effect is good, the use amount of a flocculant can be greatly reduced, the flocculation rate can be increased, and the microbial flocculant complex is safeand non-toxic, has no secondary pollution, is a water treatment agent with good performance, and can be used for treating urban sewage, industrial wastewater and the like.

The invention discloses a sewage treatment agent, which comprises the following raw materials in parts by weight: 1-5 parts by weight of microorganisms, 10-40 parts by weight of acidic substances, 25-40 parts by weight of starch and 30-50 parts by weight of pump Stolite; the microorganism is one or both of Rhodococcus erythropolis and Corynebacterium. The sewage treatment agent prepared by using microorganisms, acidic substances, starch, and rectorite in the present invention can produce clear, transparent, colorless and odorless water body, and its various indicators can meet GB18918‑2002 "Urban Sewage Treatment Plant Pollutant Discharge Standard" 》First-class B standard, some of the indicators can reach the first-class A standard, and there is no secondary pollution problem.

The Rhodococcus erythropolis related by the invention is Rhodococcus erythropolis SDUZAWQ, which is stored in 'China microorganism strain preservation management committee common micro organism center' at March 1st, 2004, and whose storage number is CGMCC NO. 1922. The strain is obtained by separating from sewage water from oil-extraction plant or soil. The cell culture fluid of the strain can high effectively remove sulfureous in dibenzothiophene, 4, 6-dimethyl-dibenzothiophene, 4-methyl-dibenzothiophene, benzothiophene, 5-methyl-benzothiophene by oxidative scission of C-S bond. Mixed sulfureous source of dibenzothiophene and sodium sulfate is used for culturing the Rhodococcus erythropolis SDUZAWQ, and the obtained resting cell culture fluid can effectively remove the sulfureous contained in diesel oil which has been subject to hydrofining.

The invention discloses a functionalized unnatural chiral amino acid compounds with quaternary carbon centers and a preparation method thereof. In the invention, a Rhodococcus erythropolis AJ270 microbial system is taken for catalytic hydrolysis of prochiral malonamide compounds with different substituent groups so as to obtain corresponding unnatural amino acids with an amide group. The dosage of the Rhodococcus erythropolis can be adjusted according to the dosage of a substrate. The reaction solvent is a common buffer solution with a pH value of 6.0-8.0, and the reaction is performed at 20-37DEG C for 0.1-120h. The Rhodococcus erythropolis microbial catalytic system has the characteristics of fermentable cultivation and convenient preservation. The method for preparing monoamide carboxylic acid and dicarboxylic acid through bioconversion has the characteristics of simple operation, high efficiency reaction, mild reaction conditions, high enantioselectivity, easy separation of products, and high product purity, and can be used for synthesis of other unnatural chiral amino acids with a quaternary carbon center.

The invention discloses a method for converting podophyllotoxin to podophyllic acid and picropodophyllin, comprising the following steps: the substrate podophyllotoxin solution is added during the fermentation process of microorganism pseudomonas aeruginosa, bacillus, rhodococcus erythropolis, clostridium, corynebacterium pekinense, hay bacillus, erwinia uredovora or bending pseudomonas for carrying out biotransformation reaction, thus obtaining biotransformation matrix with the podophyllic acid and picropodophyllin. The invention also discloses a method for separating the podophyllic acid and picropodophyllin from the obtained biotransformation matrix, comprising the following steps: the macroporous absorbent resin column is adopted to carry out initial separation on the biotransformation matrix and gel column chromatography is adopted to carry out fine separation, thus respectively obtaining the picropodophyllin and the podophyllic acid. In the invention, modification is carried out on the picropodophyllin structure by microbiological transformation, thus obtaining the picropodophyllin and the podophyllic acid, in addition, the conversion rate of the substrate is high, the operation is easy, the reaction conditions are moderate, the generated waste is little, the separation process is simple and the yield is high.

The invention relates to the field of garbage disposal, in particular to a complex microbial agent and an application thereof in aerobic repair of landfill. The complex microbial agent contains an type-I complex microbial agent and/or a type-II complex microbial agent, wherein the type-I complex microbial agent is prepared from streptomyces badius, lactobacillus crispatus, bacillus megatherium, bacillus subtilis, bacillus licheniformis and active dry yeast; the type-II complex microbial agent is prepared from phanerochaete chrysosporium, bacillus cereus, paracoccus denitrificans, candida utilis and rhodococcus erythropolis. The complex microbial agent is an efficient microbial agent prepared by massive screening and compounding according to specificity of nature change of garbage components, environmental factors and pollutants in the aerobic repair process of the landfill. The two types of microbial agents are added selectively in the aerobic repair process of the landfill by stages,and by means of function specificity of the microbial agents, the aerobic repair operating effect of the landfill is enhanced, the treatment cycle is shortened, and the operating cost is reduced.

The invention discloses non-natural chiral amino acid compounds and a preparation method thereof. The non-natural chiral amino acid compounds are shown as a formula III. Raw materials for preparing the non-natural chiral amino acid compounds provided by the invention are obtained by using a Rhodococcus erythropolis AJ270 microbial system to catalytically hydrolyze prochiral malonamide compounds with different substituents. The dosage of Rhodococcus erythropolis AJ270 thalluses can be adjusted according to the dosage of substrate. Reaction solvent is commonly used buffer solution with a pH (potential of hydrogen) value being 6.0-8.0, the temperature is 20-37DEG C and reaction time is 0.1-120 hours. The Rhodococcus erythropolis AJ270 microbial catalytic system has the characteristics of fermented cultivation and convenience in storage. The method for preparing chiral monoamine carboxylic acid and dicarboxylic acid through biological conversion has the advantages of simplicity and convenience in operation, high reaction efficiency, moderate reaction conditions, high enantioselectivity, easiness in product separation and high product purity, and can be used for synthesizing non-natural chiral amino acid.

The invention relates to a rhodococcus erythropolis T7-3 degrading alkane at a low temperature and an application thereof. The rhodococcus erythropolis T7-3(CGMCC No. 6104) which is one of the cold-resistant bacteria provided by the invention is obtained from a mud sample collected in a sea-bed area polluted by petroleum in the Bohai Sea, wherein the growth temperature is 4-34 DEG C, optimally 15-25 DEG C, the growth pH range is 5-10, and the sodium chloride tolerance is 0-7.5%. The rhodococcus erythropolis can grow with alkane or crude oil as a unique carbon source. The rhodococcus erythropolis has a very high capacity for degrading petroleum hydrocarbon and can degrade C12-36 n-alkanes and crude oil. The degradation rates for 1g/L alkane or 5g/L crude oil added reach over 70%. The degradation rate is 85% at 25 DEG C after shake cultivation for 7 days with 5g/L diesel oil as the unique carbon source, the degradation rate after 7 days of thin oil with 5g/L crude oil as the unique carbon source is 97.05%, and the degradation rate to thickened oil is 61.08%. Therefore, the rhodococcus erythropolis has a good application prospect in sea bioremediation.

This invention discloses Rhodococcus erythropolis XPDN strain, which is preserved in China Center for Type Culture Collection (Wuhan University, Wuhan, China) with a serial number: M205141. This invention also discloses the application of Rhodococcus erythropolis in desulfurization and denitrification for crude oil, which comprises the steps of: culturing Rhodococcus erythropolis, collecting Rhodococcus erythropolis, preparing resting cells, treating crude oil samples and inspecting. This invention has such advantages as easy operation and high desulfurization and denitrification capacity, and can degrade dibenzo thiophene and carbazole into 2-hydroxybiphenyl and ortho-aminobenzoic acid, thus have important applications in contamination treatment.

The invention discloses a wastewater degrading bacterial strain against high salts and high COD, a cultivation method, a bacterial solution and an application. The bacterial strain is isolated from sludge near a wastewater drain outlet and is obtained by conducting cyclic acclimatization. The bacterial strain is identified as Rhodococcus erythropolis. The bacterial strain can effectively degrade COD in wastewater (the maximum load of COD in inlet water can reach 12,000 mg/L) under environment of higher salinity (the maximum load of salt concentration in wastewater is 80 g/L), achieves a COD removal rate up to 62%, and allows outgoing water to meet requirements of the national sewage comprehensive emission standards on sewage discharged into a collection network of urban sewage treatment plants. The treatment process of the invention is simple in operations, good in outgoing water quality, low in treatment cost, and can be applied for engineering on the basis.

The invention relates to a mixed microbial preparation for recovering soil polluted by composite pollutant oil in a biology manner and a manufacture method of the mixed microbial preparation. The mixed microbial preparation is made by mixing six bacterial strains including acinetobacter calcoaceticus, klebsiella pneumonia, enterobacter cancerogenus, pseudomonas stutzeri, rhodococcus erythropolis, and pseudomonas putida with one of rhodococcus, acinetobacter, gordonia, sphingobacterium, novosphingobium capsulatum, acinetobacter, and xanthomonas at a ratio of 1:1:2:2:1:1:1:1 and is used for recovering oil-polluted soil.

The invention discloses a microbial sewage treatment agent. The microbial sewage treatment agent is prepared from the following raw materials by weight: 15-25 parts of zeolite powder, 25-35 parts of activated carbon, 5-15 parts of potassium feldspar, 5-15 parts of lauryl trimethyl ammonium bromide, 5-15 parts of magnesium sulfate, 1-5 parts of Rhodococcus erythropolis, 1-5 parts of Phanerochaete chrysosporium, 1-5 parts of Aspergillus sojae, 1-5 parts of Thiobacillus and 5-15 parts of potassium phosphate. The microbial sewage treatment agent of the invention is prepared from environmentally friendly raw materials, and is high in the specific surface area of an inorganic filler, high in activity, high in dispersibility and strong in adsorptivity; at the same time, the microbial sewage treatment agent can play the roles of demulsification, flocculation, bridging and foaming; and when applied to treatment of sewage from textile mills, the microbial sewage treatment agent has obvious treatment effect and good stability and is hardly affected by the environment.

The present invention belongs to the technical field of soil environments, and specifically relates to a microbial soil improvement agent for petroleum polluted soil. The microbial soil improvement agent comprises the following raw materials: Paxillus involutus Hypha, Hebeloma mesophaeusm, Acinetobacter calcoaceticus, Sphingomonas paucimobilis, pyrene degrading bacteria, Glomus etunicatum, Cephalosporium roseum, Aureobasidium pullulans, nitrilase, long-chain alkane degradation enzyme, integration membrane di iron alkane hydroxylase, phenylalanine deaminase, arginine dihydrolase, Rhodococcus erythropolis, pseudomonas aeruginosa, sodium oleate, thiamine hydrochloride, calcium pantothenate, oyster shell powder, vermiculite powder, paspalum wettsteinii hackel powder, kentucky eupatorium fortunei powder, miscanthus floridulus powder, medicago sativa powder, pig manure granules, goat manure granules, gamma-polyglutamic acid, malic acid, citric acid, and Nostocales powder. According to the present invention, various types of the microorganisms in the improvement agent synergistically coordinate, and co-act with the carrier raw materials; and with the application of the improvement agent to treat different types of crude oil polluted soil in the Shengli oil field, the total removal rate of the petroleum hydrocarbon in the polluted soil achieves 77.68-89.76%.

The invention provides a novel bacterial strain - Rhodococcus erythropolis WZ010 and application thereof in selectively oxidizing racemic aromatic alcohol to prepare R-aromatic alcohol and asymmetrically reducing aromatic ketone to produce S-aromatic alcohol. The bacterial strain is preserved in China Center for Type Culture Collection, Wuhan University, Wuhan, China, the postcode is 430072, the preservation number is CCTCC No: M2011336, and the preservation date is 29 September, 2011. The invention mainly has the advantages that: the invention provides the novel bacterial strain with high stereoselectivity and high enzymatic activity, the R-aromatic alcohol and the S-aromatic alcohol can be obtained at the same time by selective oxidation and asymmetric reduction with the bacterial strain, the reaction specificity and catalytic activity of the bacterial strain are high, the production cost is greatly reduced, moreover, environment pollution is little, and the bacterial strain is suitable for industrialized production.