Wastewater degrading bacterial strain against high salts and high COD, cultivation method, bacterial solution and application

A technology for degrading wastewater and degrading bacteria, applied in chemical instruments and methods, methods based on microorganisms, methods using microorganisms, etc., can solve problems such as aggravating fresh water, increasing treatment costs, and microbial death, and achieve the effect of improving screening efficiency

Active Publication Date: 2020-05-08
北京翰祺环境技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] When the salinity in the environment exceeds a certain critical range, the growth of microorganisms will be inhibited and poisoned, the extracellular osmotic pressure will increase with the increase of salt concentration, the intracellular pressure will be smaller, and the water in the cells will flow out , leading to the separation of plasmodium and the death of microorganisms; in addition, salting out occurs in stra

Method used

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  • Wastewater degrading bacterial strain against high salts and high COD, cultivation method, bacterial solution and application
  • Wastewater degrading bacterial strain against high salts and high COD, cultivation method, bacterial solution and application
  • Wastewater degrading bacterial strain against high salts and high COD, cultivation method, bacterial solution and application

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Embodiment 1

[0038] The domestication and screening steps of wastewater-degrading bacterial strains resistant to high salt and high COD are as follows:

[0039] (1) Sludge was collected near the waste water outlet of Lucheng Tianji Coal Chemical Group Co., Ltd., Shanxi Province, and the sludge sample was inoculated on beef extract liquid medium with a sulfate concentration of 10 g / L at an inoculum volume of 5%. , enriched and cultured on a shaker at 30°C for 4 days to obtain a primary acclimatization culture medium;

[0040] (2) According to the inoculum amount of (1), inoculate the first-level domestication culture medium on the beef extract liquid medium with a sodium sulfate concentration of 20g / L, enrich and culture it on a shaker at 30°C for 4 days, and obtain the second-level domestication culture medium ;

[0041] (3) According to the inoculum amount in (1), inoculate the previous-level acclimatization culture medium into the new beef extract liquid medium containing sodium sulfate...

Embodiment 2

[0045] Molecular biological identification of strains

[0046] After screening and domestication, the sodium sulfate-resistant strain was obtained, which was positive by Gram staining. When cultured on a plate, the colony was round, with a smooth surface and slightly raised, regular edges, and red color. Microscopic examination showed that the bacteria were spherical. It was identified as Rhodococcus erythropolis by 16S rRNA ( Rhodococcus erythropolis ), name it Rhodococcus erythropolis. HQC-1, the conditions for strain survival and growth and reproduction are temperature 20-55°C, pH 6.0-8.0. See the appendix for the sequence list in the 16rRNA identification of the strain of the present invention.

Embodiment 3

[0048] Rhodococcus erythropolis. The cultivation method of HQC-1

[0049] The above-mentioned isolated strains were cultured in beef extract liquid medium, which consisted of: 0.3% beef extract; 1.0% peptone; 0.5% sodium chloride; 0.1% potassium dihydrogen phosphate; 0.05% magnesium sulfate; pH 7.2, OD of the strain 600 Trends over time (i.e. growth curves of strains) such as figure 1 As shown, in the process of cultivating for 30 hours, it was found that the growth trend of the strain was better in the period of 6-18 hours. Therefore, the corresponding OD 600 The bacterial solution with a value of 0.4-1.8 is a bacterial solution, and the effective viable bacterial content can be obtained ≧2×10 9 individual / mL.

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Abstract

The invention discloses a wastewater degrading bacterial strain against high salts and high COD, a cultivation method, a bacterial solution and an application. The bacterial strain is isolated from sludge near a wastewater drain outlet and is obtained by conducting cyclic acclimatization. The bacterial strain is identified as Rhodococcus erythropolis. The bacterial strain can effectively degrade COD in wastewater (the maximum load of COD in inlet water can reach 12,000 mg/L) under environment of higher salinity (the maximum load of salt concentration in wastewater is 80 g/L), achieves a COD removal rate up to 62%, and allows outgoing water to meet requirements of the national sewage comprehensive emission standards on sewage discharged into a collection network of urban sewage treatment plants. The treatment process of the invention is simple in operations, good in outgoing water quality, low in treatment cost, and can be applied for engineering on the basis.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, more specifically, to a high-salt and high-COD wastewater-degrading bacterial strain, a culture method, a bacterial liquid and an application. Background technique [0002] COD (Chemical Oxygen Demand) is chemical oxygen demand, also known as chemical oxygen demand, which is used to reflect the amount of reducing substances that need to be oxidized in sewage. It is used in the research of river pollution and industrial sewage properties and the operation and management of sewage treatment plants. Among them, it is an important parameter of organic pollution that can be determined quickly. With the significant acceleration of China's modernization process, large-scale wastewater with high salinity, such as industrial wastewater, agricultural water, and urban sewage, is directly or indirectly discharged into nature, causing varying degrees of pollution to the atmosphere, soil, water, and o...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12N1/36C02F3/34C12R1/01
CPCC02F3/34C12N1/02C12N1/20C12N1/36C12N1/205C12R2001/01
Inventor 王晓阳汪德罡谢晓朋
Owner 北京翰祺环境技术股份有限公司
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