Rhodococcus erythropolis and its uses in crude oil desulfuration

A technology of Rhodococcus erythropolis and crude oil, applied in bacteria and other directions, can solve problems such as increasing desulfurization cost, and achieve the effect of great application prospects

Active Publication Date: 2006-05-03
上海道多生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, in the crude oil smelting industry, crude oil is divided into various components and

Method used

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  • Rhodococcus erythropolis and its uses in crude oil desulfuration
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  • Rhodococcus erythropolis and its uses in crude oil desulfuration

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Example 1: Screening of Rhodococcus erythropolis CCTCC M205068 bacterial strain

[0050] Various contaminated soils near oil fields or refineries were collected. Each weighed 5 grams of the above soil was added to 50 mL of liquid inorganic salt basic medium containing 0.01% dibenzothiophene by mass volume ratio, and cultured with shaking at 30 ° C for 48 hours at a speed of 250 rpm, and then 10 Inoculate the inoculation amount of % volume ratio into a new liquid inorganic salt basal medium containing 0.01% dibenzothiophene by mass volume ratio, at 30°C, under the condition of rotating speed 250 rpm, shake culture for 48 hours, and then continue Transfer, repeat this process 3 to 5 times. Dilute the cultured bacterial solution 1,000,000 times and spread it on the solid inorganic salt basic medium containing agar with a mass volume ratio of 1.6% and adding dibenzothiophene with a mass volume ratio of 0.01%, and culture it statically at 30°C for 72 hours . Select the gr...

Embodiment 2

[0056] Embodiment 2: Extraction of the 16SrDNA gene of the above-mentioned Rhodococcus erythropolis CCTCC M205068 bacterial strain

[0057] Cultivate 5ml of bacterial culture to saturation, take 1.5ml of culture, and centrifuge at 6000 rpm for 2 minutes; add 565μl of TE buffer to the precipitate, and the formula of TE buffer is as follows: 10mmol / L Tris (Tris), 1mmol / L ethylenediaminetetraacetic acid (EDTA), adjust the pH to 8.0 with hydrochloric acid, resuspend it by blowing repeatedly with a pipette, and add 30 μl of 10% sodium dodecylsulfonate ( SDS) and 5μl 20mg / mL proteinase K, mix well, and incubate at 37°C for 1 hour; add 100μl, 5mol / L NaCl, mix well, then add 80μl CTAB / NaCl solution, the CTAB / NaCl solution formula As follows: cetyltriethylammonium bromide (CTAB) with a mass volume ratio of 10% was dissolved in 0.7mol / L NaCl, mixed evenly, and incubated at 65°C for 10 minutes; an equal volume of chloroform / iso Pentanol, mix well, centrifuge at 12,000 rpm for 5 minutes,...

Embodiment 3

[0083] Embodiment 3: The method for removing organic sulfur in crude oil by using resting cells of Rhodococcus erythroflates, wherein the volume ratio of added crude oil to water phase is 1:10, and the treatment temperature is 25°C

[0084] The present invention utilizes the application of Rhodococcus erythropolis in crude oil desulfurization, and the sequence of steps involved in its desulfurization method is as follows:

[0085] (1) Strain selection: choose Rhodococcus erythropolis CCTCC M205068;

[0086] (2) Slant culture: Inoculate the bacteria on a solid slant inorganic salt basic medium containing 1.6% agar with a mass volume ratio of 0.005% dibenzothiophene, at 25° C. Cultivate for 42 hours;

[0087](3) Seed culture: the bacterial strain that step (2) is cultivated, connects 2 rings with inoculation loop under aseptic condition in 100mL and contains the liquid inorganic salt basic medium that mass volume ratio is 0.005% dibenzothiophene, 25 Under the condition of ℃, s...

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Abstract

The invention discloses a Rhodococcus erythropolis preserved in CRCCC in Wuhan University in 6,20,1005 with number of M205068. It also discloses its application in crude oil desulfurization that comprises: (1) slant cultivation; (2) seed cultivation; (3) enlarge cultivation; (4) collecting thalli; (5) preparing diapause cell; (6) treating crude oil sample; (7) sample detection. This invention has high desulfurization ratio with simple operation and bright application future.

Description

technical field [0001] The invention relates to a rhodococcus strain and a method for removing organic sulfur in fossil fuels, in particular to a rhodococcus strain and its application in crude oil desulfurization. Background technique [0002] The organic and inorganic sulfur contained in fossil fuel coal and petroleum is an important source of environmental pollution. When these sulfides are burned, they emit a large amount of toxic gases such as sulfur dioxide, and the resulting large-scale acid rain seriously pollutes the air and water sources and destroys the ecology. Balance, endangering human survival. Information Disclosure China's SO 2 Its emissions account for about 15% of the world, ranking third in the world after the United States and the former Soviet Union. 2 major emitters. Japanese SO 2 The annual emission is only China's SO 2 1 / 15 of the emissions. In order to reduce pollution, countries around the world have strict regulations on the exploitation of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12S1/02
Inventor 许平马翠卿于波李福利冯进辉蔡晓凤刘晓勇王霞杨春玉陶飞王颖周文娟
Owner 上海道多生物科技有限公司
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