Functionalized unnatural amino acids with quaternary carbon centers and biocatalytic desymmetrization preparation method thereof

A compound and catalytic hydrolysis technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as poor selectivity and harsh chemical transformation requirements
CN103288674AInactive Publication Date: 2013-09-11INST OF CHEM CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF CHEM CHINESE ACAD OF SCI
Publication Date
2013-09-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a functionalized unnatural chiral amino acid compounds with quaternary carbon centers and a preparation method thereof. In the invention, a Rhodococcus erythropolis AJ270 microbial system is taken for catalytic hydrolysis of prochiral malonamide compounds with different substituent groups so as to obtain corresponding unnatural amino acids with an amide group. The dosage of the Rhodococcus erythropolis can be adjusted according to the dosage of a substrate. The reaction solvent is a common buffer solution with a pH value of 6.0-8.0, and the reaction is performed at 20-37DEG C for 0.1-120h. The Rhodococcus erythropolis microbial catalytic system has the characteristics of fermentable cultivation and convenient preservation. The method for preparing monoamide carboxylic acid and dicarboxylic acid through bioconversion has the characteristics of simple operation, high efficiency reaction, mild reaction conditions, high enantioselectivity, easy separation of products, and high product purity, and can be used for synthesis of other unnatural chiral amino acids with a quaternary carbon center.
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Description

technical field

[0001] The invention relates to a functionalized non-natural amino acid with a quaternary carbon center and a biocatalytic desymmetry preparation method thereof. Background technique

[0002] Amino acid compounds are the most widely existing compounds in living organisms, and are important components of living organisms, which play a very important role in the process of life. Because of its similar structure to natural amino acids, unnatural amino acids can often be used as substitutes for natural amino acids to study the role of different amino acids in life processes. Unnatural amino acids have shown certain promise as synthetic raw materials for some growth factor receptor proteins. drug activity. Currently commercialized unnatural amino acids include 2-Amino-2-methyl-3-phenylpropanoic acid (sold by Pure Chemistry Scientific Inc, USA) and 2-Amino-2-methyl-4-pentenoic acid (sold by hexagon labs, USA) .

[0003] Biocatalysis is by far the most efficient,...

Claims

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