214 results about "Catalytic hydrolysis" patented technology

The invention discloses a fusion enzyme capable of efficiently degrading PET (polyethylene terephthalate) plastic and application of the fusion enzyme. The invention provides a recombinant fusion enzyme. The recombinant fusion enzyme comprises a mutant KL-MHETase of thermophilic carboxylesterase Est30 and a PET (Polyethylene Terephthalate) degrading enzyme FAST-PETase, the amino acid sequence of the mutant KL-MHETase of the thermophilic carboxylesterase Est30 is as shown in SEQ ID NO. 3; the amino acid sequence of the FAST-PETase is as shown in the 284th to 546th amino acids of SEQ ID NO.1 (Sequence Identifier Number 1). The fusion enzyme is used as a biocatalyst, the activity of catalyzing hydrolysis of MHET at 50 DEG C is 52.6 times that of FAST-PETase, and the fusion enzyme has high enzyme activity and thermal stability. PET degradation is catalyzed at the temperature of 50 DEG C, the conversion rate of PET is 90% after 48 hours of reaction, and meanwhile, the TPA content in the product is close to 100%.

The invention discloses a high-activity and high-thermal-stability MHET hydrolase mutant and application thereof. The invention provides a mutant protein of thermophilic carboxylesterase, which is shown as 1) or 2): 1) the protein shown as 1) is a protein obtained by mutating amino acid residues at the 130th site and the 171th site of wild thermophilic carboxylesterase shown as SEQ ID NO.2 or a sequence corresponding to the SEQ ID NO.2 and keeping other amino acid residues unchanged; (2) the protein is obtained by mutating amino acid residues at the 127th site, the 130th site and the 171th site of wild thermophilic carboxylesterase and keeping other amino acid residues unchanged. Experiments prove that the activity of the modified mutant for catalyzing MHET hydrolysis at the temperature of 50 DEG C is improved by 95 times compared with that of a wild type, and the modified mutant has higher enzyme activity and thermal stability and is the first enzyme capable of efficiently catalyzing MHET hydrolysis at the temperature of 50 DEG C at present.

The invention belongs to the field of biological catalysis, and discloses a high-activity PET hydrolase mutant and application thereof. The mutant of the thermophilic PET hydrolase provided by the invention is as shown in 1), 2) or 3): 1) a protein corresponding to an amino acid sequence SEQ ID NO.4; 2) a protein corresponding to the amino acid sequence of SEQ ID NO.6; and 3) a protein corresponding to the amino acid sequence of SEQ ID NO.8. Experiments prove that the modified mutant shows higher catalytic efficiency on PET hydrolysis and can keep activity for a long time at the optimal temperature for PET depolymerization. The activity of the mutant protein BhrM3 for catalyzing PET hydrolysis is improved by 1.82 times compared with that of a wild type and is 1.22 times that of efficient PET hydrolase LCC-ICCG, and the protein melting temperature reaches 92.9 DEG C.

The invention belongs to the technical field of pharmaceutical wastewater treatment, and particularly relates to a device and a method for treating antibiotic production wastewater by coupling catalytic hydrolysis and catalytic degradation. The device comprises a catalytic hydrolysis reactor and an electro-catalysis reactor, the two reactors are connected in series through a pipeline with a water stop valve, the catalytic hydrolysis reactor is divided into a plurality of compartments by water baffles, and each compartment is filled with a catalytic porous hydrolysis filler; and a cathode, an anode and a stirrer are arranged in the electro-catalytic reactor. By utilizing the porous hydrolysis filler in the device, selective catalytic hydrolysis of high-concentration antibiotics in wastewater is completed by controlling the hydraulic retention time, the anodic oxidation efficiency is improved by virtue of the high contact reaction area of the porous blocky anode, and efficient mineralization degradation of residual antibiotics and hydrolysates thereof is realized. The treatment device and method for the antibiotic production wastewater are good in selective hydrolysis effect, high in mineralization degradation efficiency, easy and convenient to operate and maintain and low in energy consumption.

The invention discloses a phenanthridine-6-formaldehyde derivative and a preparation method and application thereof.The preparation method comprises the steps that 2, 4, 5, 6-tetra (9-carbazolyl)-isophthalonitrile serves as a photocatalyst, 1-azabicyclo [2.2. 2] octane serves as a hydrogen atom transfer catalyst, a biphenyl isocyanide compound, the photocatalyst, the hydrogen atom transfer catalyst and an alkali additive are jointly dissolved in 1, 3-dioxolane, the mixture is stirred to be uniform, and the phenanthridine-6-formaldehyde derivative is obtained. And carrying out free radical tandem cyclization and acid-catalyzed hydrolysis reaction to obtain the phenanthridine-6-formaldehyde derivative. The invention also provides a specific reaction equation. According to the developed synthesis method of the phenanthridine-6-formaldehyde derivative, the compound can be efficiently and conveniently prepared, the technical bottlenecks of harsh reaction conditions and tedious steps in a traditional process are successfully overcome, raw materials which are low in price and easy to obtain are adopted, operation is carried out under mild conditions, the synthesis method has the outstanding advantages of being high in yield, safe and simple to operate, environmentally friendly and the like, and the method is suitable for industrial production. Good industrial application potential is shown.

The invention provides a carbonyl sulfide hydrolysis catalyst and a preparation method thereof. The preparation method comprises the following steps: respectively preparing a K-containing alkaline solution and a samarium salt aqueous solution for later use; dipping the catalyst carrier in a K-containing alkaline solution, and then carrying out a first reaction under a certain condition to obtain a K-loaded catalyst precursor; immersing the catalyst precursor in a samarium salt aqueous solution for a second reaction, and further loading samarium; the carbonyl sulfide hydrolysis catalyst is prepared by the preparation method, and in the carbonyl sulfide hydrolysis catalyst, the mass fraction ratio of K to samarium is (8: 1)-(12: 1). The preparation method provided by the invention has the advantages that the raw materials are cheap and easy to obtain, the method is simple and easy to popularize, a large amount of organic solvents are prevented from being used in the preparation process, the method is more environment-friendly, and the prepared catalyst has the characteristics of higher activity, stronger stability and difficulty in poisoning when being used for catalytic hydrolysis of COS.

The invention belongs to the technical field of analytical chemistry and pesticide residue detection, and relates to a phosphate hydrolysis nano-enzyme sensing array-based p-nitrophenol pesticide identification and detection method, which comprises the following steps of: performing five groups of parallel data matrixes on each type of p-nitrophenol pesticide to distinguish single / five types of p-nitrophenol pesticides with different concentrations; establishing a discrimination model of different concentrations / types of pesticides by using the data matrix, and obtaining an HCA map and an LDA score map of the p-nitrophenol pesticides through hierarchical clustering analysis and linear discriminant analysis; after the absorbance value of the sample to be detected is measured, the sample to be detected can be identified and detected through comparison. According to the present invention, by using the inherent dynamic difference existing during the catalytic hydrolysis of different p-nitrophenol pesticides by CeO2, a plurality of reaction time points are selected to measure the absorbance change, the time-resolved sensing array fingerprint is constructed, and the type identification and the concentration quantification of a variety of p-nitrophenol pesticides and the mixture thereof are achieved by combining the pattern identification algorithm, such that the sensitivity is high, and the detection result is accurate. The measurable concentration range is 1-50 mu g / mL.

The invention discloses a composite material catalyst MFe2O4 / NH2-MIL-68 (In)-x as well as a preparation method and application thereof, and belongs to the technical field of industrial catalysis. NH2-MIL-68 (In) is used as a carrier, spinel type ferrite MFe2O4 is loaded on the NH2-MIL-68 (In) to form the composite material catalyst, M represents Co or Cu, x represents the percentage content of MFe2O4 in the composite material, the value of x is 10-60, and the unit is%. Through effective compounding of the indium-based MOF material and the spinel type ferrite MFe2O4, the advantages of large specific surface area and multiple metal active sites of the indium-based MOF material and the spinel type ferrite MFe2O4 are fully utilized, the synergistic effect of the indium-based MOF material and the spinel type ferrite MFe2O4 in the aspects of gas adsorption and catalytic hydrolysis is exerted, the COS hydrolysis catalytic activity and the reaction rate are improved, the removal rate of COS in waste gas at 120-150 DEG C can reach 92-96%, and the removal rate of COS in waste gas at 120-150 DEG C can reach 92-96%. The catalyst is suitable for medium and low temperature catalytic hydrolysis removal of carbonyl sulfide in benfenacet synthesis waste gas.

The invention discloses a composite fluorine removal agent and a preparation method thereof, and belongs to the technical field of water treatment. The preparation method of the composite fluorine removal agent comprises the following steps: dipping biomass charcoal in a lanthanum-containing solution, and calcining to obtain a La2O3 / charcoal carrier; the La2O3 / carbon carrier, nano-hydroxyapatite, MgO and magnetic Fe3O4 particles are mixed and then subjected to heat treatment, and a composite material is obtained; mixing the composite material, tetraethoxysilane and a solvent, dropwise adding ammonia water into the mixture for catalytic hydrolysis, and then annealing to obtain a SiO2-coated composite material; and mixing and granulating the SiO2 coated composite material and a binder, and then activating to obtain the composite fluorine removal agent. The method realizes the preparation of the composite fluorine removal agent which is wide in pH application range, can be recycled and can be suitable for efficient treatment of low-concentration fluorine wastewater.

The invention discloses a construction method and application of an electrochemical luminescence (ECL) biosensor based on a cell membrane hydrogel antifouling interface, and belongs to the technical fields of photo / electrochemical analysis, interface antifouling and biosensing. According to the invention, the cell membrane hydrogel is used as an efficient antifouling interface, so that non-specific adsorption of interfering proteins in a sample to be detected is hindered, and the service life of the biosensor is prolonged. Meanwhile, a luminophor copper nanosheet and a quencher MnO2 nanosheet are both introduced into the cell membrane hydrogel, in the presence of alkaline phosphatase (ALP), L-ascorbic acid-2-trisodium phosphate is catalyzed and hydrolyzed into ascorbic acid, and then MnO2 is reduced to recover ECL emission of the copper nanosheet. Therefore, the ECL signal of the biosensor is in a rising trend along with the rising of the ALP concentration. Based on this, the constructed ECL biosensor realizes sensitive detection of ALP, the linear range is 10 <-5 >-10 < 4 > U / L, and the detection limit is as low as 3.4 * 10 <-6 > U / L, which indicates that the ECL biosensor has potential application in clinical diagnosis.

The invention discloses a synthesis method of an amino-acid-based MOFs (Metal-Organic Frameworks) derived nano-enzyme, and relates to the technical field of nano-enzyme synthesis, the synthesis method of the amino-acid-based MOFs derived nano-enzyme comprises the following steps: step 1, selecting proper metal ions and organic ligands; step 2, ultrasonic dispersion; step 3, precipitate drying; and step 4, activating treatment of the nano enzyme. The MOFs-derived nano-enzyme with alkaline phosphatase activity is synthesized by taking natural amino acid as a ligand and one of ions of zirconium, copper and iron as a metal node and introducing defects by a method of adding an acidic regulator in synthesis, and the crystal structure of the nano-enzyme is not changed under the introduction of the defects, so that the alkaline phosphatase activity of the MOFs-derived nano-enzyme is improved. The MOFs-derived nano-enzyme has the advantages that the MOFs-derived nano-enzyme is modified, the catalytic hydrolysis activity of the MOFs-derived nano-enzyme on phosphate substrates is greatly improved, good substrate specificity is shown, the structure of the MOFs-derived nano-enzyme does not change after long-term incubation under acidic, alkaline and high-temperature conditions, and compared with natural enzyme, the MOFs-derived nano-enzyme has good stability, pH stability and reusability.

The invention relates to a method for reducing the anaphylaxis of natural latex by multi-enzyme cascade catalysis of rubber protein. The invention relates to the technical field of catalysis of rubber protein hydrolysis, in particular to a method for reducing the anaphylaxis of natural latex by thoroughly hydrolyzing rubber protein through multi-enzyme cascade catalysis. The method for reducing the anaphylaxis of the natural latex by catalyzing the rubber protein through the synergistic effect of multiple enzymes from the perspective of industrial production comprises the following steps: adding an enzyme A and an enzyme B into the natural latex for reaction so as to reduce the anaphylaxis of the natural latex, the enzyme A is alkaline protease; and the B enzyme is at least one of aminopeptidase, carboxypeptidase B, carboxypeptidase A and carboxypeptidase Y. According to the method disclosed by the invention, the enzyme A-endopeptidase and the enzyme B-exopeptidase are subjected to multi-enzyme-stage combined treatment and concerted catalysis, so that the enzyme A-endopeptidase and the enzyme B-exopeptidase are completely hydrolyzed or are completely hydrolyzed into more amino acid substances and short-chain peptides (oligopeptides) as far as possible, and the amino acid substances and the short-chain peptides (oligopeptides) are retained in latex. Protein is highly hydrolyzed into amino acid with higher safety, so that the possibility of I-type anaphylactic reaction caused by protein allergens is greatly reduced or even eliminated.

The invention discloses a gamma-glutamyl transpeptidase catalytic hydrolysis induced charge upset polymer, which contains a gamma-glutamyl transpeptidase response element part and a fluorescent probe corresponding fragment part, the invention also discloses a preparation method of the polymer for turning charge by catalytic hydrolysis of gamma-glutamyltranspeptidase and an application method of the polymer in the field of tumor imaging. According to the present invention, the gamma-glutamyltranspeptidase catalytic hydrolysis electric charge overturning polymer is adopted as the response type tumor imaging probe, such that the electric charge can be converted from the negative or neutral state to the positive state so as to achieve the rapid uptake of the positively charged polymer by the tumor cells so as to achieve the rapid spraying imaging, and the probe response after the uptake is adopted so as to achieve the rapid spraying imaging; the imaging effect of the probe on the tumor is greatly improved, and the probe has important significance in solid tumor imaging for assisting a doctor in surgical resection.

The invention relates to the technical field of wastewater treatment, and discloses a modular electro-catalytic hydrolytic acidification reaction device and a wastewater treatment method. The modularized electro-catalysis hydrolytic acidification reaction device comprises a hydrolytic acidification area, an electro-catalysis area, a backflow system and a solar power supply system, the backflow system comprises a backflow pipe and a backflow channel, outlet water of the hydrolytic acidification area flows into the electro-catalysis area through the backflow pipe, and outlet water at the tail end of the electro-catalysis area flows into the hydrolytic acidification area through the backflow channel, the electro-catalysis area comprises a plurality of electro-catalysis modules which are electrically connected, each electro-catalysis module comprises an electrode assembly and an aeration device, each electro-catalysis module is divided into a micro-aerobic area and an anoxic area, and the micro-aerobic areas and the anoxic areas are alternately and continuously arranged. When the reaction device is used for treating wastewater, the function of synchronous nitrification and denitrification efficient nitrogen removal can be realized, the organic matter removal efficiency in the hydrolytic acidification stage is improved, and the biodegradability of sewage is enhanced.

The present application relates to a kind of anti-poisoning catalyst for blast furnace gas organic sulfur hydrolysis and its preparation method.Each component is as follows in percentage by weight: active component K2CO3 4-12%;Active component Cs2CO3 2-5%;Catalyst aid MoO3 3-8%;Catalyst aid La2O3 0.5-2%;Molding aid SiO2 1-5%;And composite metal oxide carrier Al2O3-TiO2 68-89.5%, the pore size of the composite metal oxide carrier Al2O3-TiO2 is three channel distribution.First, composite metal oxide carrier is prepared, and after being formed with silicon dioxide, active component and catalyst aid are impregnated to prepare catalyst.The catalyst prepared by the present application has excellent catalytic hydrolysis activity for organic sulfur in blast furnace gas, and has anti-sulfur, anti-chlorine and anti-oxygen poisoning performance and has persistent catalytic oxidation activity.

The invention discloses a TAPC esterase mutant with improved thermal stability as well as a construction method and application of the TAPC esterase mutant. The TAPC esterase mutant is obtained by mutating on an amino acid sequence as shown in SEQ ID NO.2, and a mutation site is selected from one or a combination of more than one of S275Y, S326F, S326M, G266M, R321G, H385L, S84E and H297L. The TAPC esterase mutant provided by the invention comprises eight single-site mutants and two double mutants, and compared with wild type TAPC esterase, the mutant has a longer half-life period at 49 DEG C; the double mutant has a better effect, and the half-life period of the double mutant is about 13 times of that of wild TAPC esterase. The TAPC esterase mutant obtained by the construction method provided by the invention has better thermal stability, shows higher thermal stability when an ester-containing compound is catalytically hydrolyzed at a higher temperature, and has higher industrial application potential.

The invention relates to a catalytic hydrolysis method of waste epoxy resin, which comprises the following steps: putting the waste epoxy resin into a reaction kettle, adding ultrapure water, adding a small amount of sodium dodecyl diphenyl ether disulfonate surfactant and a boron trifluoride acetic acid catalyst, and carrying out hydrolysis reaction to realize catalytic degradation of the epoxy resin. Degradation products can be converted into epoxy monomers and anhydride monomers, so that cyclic utilization is realized. Compared with the prior art, the degradation method provided by the invention is not only green and safe, but also high in degradation efficiency, solves the problem that the waste epoxy resin cannot be recycled, saves resources, meets the green development requirement, and has practical engineering application value.

The present application relates to the field of treatment of biomass organic waste for efficient resource recovery, and in particular to a treatment method for biomass organic waste and a water-soluble organic fertilizer obtained therefrom. The treatment method for biomass organic waste includes crushing the biomass organic waste, adding a catalyst made from various metal compounds, heating the mixture to 140℃ to 160℃, and applying a pressure of 0.8 MPa to 1.4 MPa, followed by a catalytic hydrolysis reaction for 30 min to 60 min to obtain small molecule organic compounds; and adding mineral elements into the obtained small molecule organic compounds, and obtaining a water-soluble organic fertilizer through ultrasonic chelation. The method can efficiently, safely, and cost-effectively process biomass organic waste, and can also produce a water-soluble organic fertilizer that is beneficial for plant growth.

The invention discloses a method for catalyzing hydrolysis of hexafluorophosphate radicals by using a bifunctional solid acid material, the main active sites of the bifunctional solid acid material in the method are a Brnsted acid site formed by combining protonated sulfate radicals (HSO4 <->) with hydroxyl groups on the surface of TiO2 and a Ti < 4 + > (Lewis acid) site enhanced by induction of the Brnsted acid site, and in the catalytic hydrolysis process, the Brnsted acid site and the Ti4 < + > (Lewis acid) site can be used for catalyzing hydrolysis of the hexafluorophosphate radicals by using the bifunctional solid acid material. Ti4 < + > (Lewis acid site) generates strong adsorption and activation effects on PF6 <->, adjacent HSO4 <-> (Brnsted acid site) and PF6 <-> generate H.. F-P interaction, and the two sites apply a synergistic stretching effect on a P-F bond from different directions, so that a stable regular octahedron structure of PF6 <-> is greatly damaged, a reaction energy barrier of P-F bond breakage is remarkably reduced, efficient hydrolysis of PF6 <-> is realized, and the reaction time is shortened. The method has the advantages that the hydrolysis efficiency is high, the hydrothermal stability is good, extreme acid and alkali environments are not needed, the bifunctional catalyst is easy to regenerate and the like, and a brand new technical path and a solution are provided for harmless treatment of the hexafluorophosphoric acid-containing wastewater.

This invention proposes a method for synthesizing glyoxylic acid from methyl glycolate. Using methyl glycolate and oxygen / water as raw materials, glyoxylic acid is produced in a one-step process. This invention employs a novel catalyst based on ZSM-5 molecular sieve, modified with transition metals and organic bases, and finally extruded into strips. This catalyst can catalyze both oxidation and hydrolysis. This route is characterized by its simplicity, high reactivity, and high catalytic selectivity, making it a green route for glyoxylic acid synthesis.

Provided in the present disclosure are a yeast protein having an antibacterial function and a preparation method therefor. The method includes: S1, performing liquid fermentation on a Saccharomyces cerevisiae strain, and then performing separation to obtain a yeast milk; S2, acidifying the yeast milk to obtain an acidified yeast milk; and optionally performing autolysis, or exogenous enzyme catalytic hydrolysis, or mechanical crushing on the yeast milk to obtain a cell wall emulsion; S3, mixing the acidified yeast milk and the optional cell wall emulsion and performing autolysis to obtain an autolysis mixed solution; S4, performing first enzymolysis with compound protease; S5, performing second enzymolysis with seminase; and performing third enzymolysis with β-glucanase and cellulase to obtain a yeast protein emulsion; and S6, evaporating and concentrating the yeast protein emulsion to obtain the yeast protein. According to the method, a yeast protein having a broad-spectrum antibacterial function can be prepared.

The invention provides a transformer oil acetylene degassing-free detection method and system based on click chemistry, and relates to the technical field of gas detection analysis, a first product generated by a first click chemical reaction and a second product generated by a second click chemical reaction are used as catalysts to trigger a subsequent same cascade chemical amplification reaction, and the detection result is accurate. A single product molecule can catalytically hydrolyze a large number of acid-sensitive substrates, the conductivity of a mixed solution is obvious and can be measured and changed, accurate detection of acetylene with extremely low concentration is realized, and meanwhile, acetylene detection is calculated by utilizing a second click chemical reaction synchronously generated by an internal standard reagent and a triggering agent and independent of an acetylene concentration detection process, so that the accuracy of acetylene detection is improved. According to the method, the signal ratio is obtained by calculating the ratio of the first electric signal value to the second electric signal value instead of the single first electric signal value, interference factors are offset, online self-correction of acetylene detection is realized, and high consistency and accuracy of detection results under different times and different batches of reagents are ensured.

The invention belongs to the technical field of biological genetic engineering, and particularly relates to a bacillus subtilis sourced esterase Est909 for degrading phthalate, a coding gene and application of the bacillus subtilis sourced esterase Est909. The amino acid sequence of the esterase Est909 is as shown in SEQ ID NO. 1, and the nucleotide sequence of the coding gene of the esterase Est909 is as shown in SEQ ID NO. 2. The invention specifically discloses a bacillus subtilis source esterase Est909 coding gene and construction of an escherichia coli expression vector carrying the gene, and also discloses application of the bacillus subtilis source esterase Est909 in hydrolysis of various diesters of phthalate and monoesters of phthalate. And the catalytic hydrolysis efficiencies of diisobutyl phthalate, dibutyl phthalate and bis (2-ethylhexyl) phthalate of the homologous enzyme PAEIIIase are respectively 1.26 times, 1.30 times and 2.17 times of the catalytic hydrolysis efficiencies of the homologous enzyme PAEIIIase, and the catalytic hydrolysis efficiencies of the homologous enzyme PAEIIIase are respectively 1.30 times, 1.30 times and 2.17 times of the catalytic hydrolysis efficiencies of the homologous enzyme PAEIIIase. The invention firstly provides the III-type enzyme Est909 for efficiently catalyzing and degrading the phthalate and the coding gene of the III-type enzyme Est909.

The present application belongs to the technical field of phosgene reaction tail gas treatment, and particularly relates to a phosgene tail gas alkali destruction device and method, which comprises the following steps: an operator passes the tail gas into a falling film absorber, the falling film absorber absorbs hydrochloric acid in the tail gas to obtain treated gas one; the treated gas one is passed into a catalytic hydrolysis box, a catalytic hydrolysis method is used to destroy phosgene in the treated gas one to obtain treated gas two; the treated gas two is passed into a multi-stage alkali destruction tower, 1% to 10% of a dilute alkali solution is sent into the top of the alkali destruction tower through a delivery pump, inorganic salt solution and treated gas three are generated through an acid-base reaction; the inorganic salt solution is sent to a high-salt wastewater treatment device for treatment, the treated gas three is sent to a burning device for treatment to remove unreacted carbon monoxide, organic matter and other combustible gases to obtain final gas which is discharged to the outside. The scheme solves the problem of waste caused by a large amount of generated high-salt wastewater.

A method for preparing a hydroxylamine aqueous solution by catalytic hydrolysis of cyclohexanone oxime belongs to the technical field of hydroxylamine aqueous solution preparation. Specifically, cyclohexanone oxime and deionized water are added to a hydrolysis kettle to completely dissolve the cyclohexanone oxime, and an appropriate amount of a bisphenol-based solid acid catalyst is added. The mixture is stirred, refluxed, and condensed to obtain a reaction solution containing hydroxylamine. An extractant is added to the reaction solution, and the organic phase in the reaction solution is separated to obtain cyclohexanone; the residual aqueous phase is centrifuged to obtain the product hydroxylamine aqueous solution and the bisphenol-based solid acid catalyst, respectively, and the catalyst is recovered and recycled. This method significantly improves the conversion rate of the catalytic hydrolysis of cyclohexanone oxime, has a simple process route, simple operation, mild reaction conditions, and low energy consumption, and is a green and environmentally friendly production process.

The invention discloses a gas decontamination and hydrolysis composite tank and a blast furnace gas fine desulfurization system, comprising a reaction tank body, wherein a gas inlet and a gas outlet are radially arranged on the reaction tank body, three mesh partitions are arranged inside the reaction tank body, the mesh partitions are arranged parallel to the center line of the reaction tank, and the gas flows radially along the tank body. The mesh partitions divide the interior of the reaction tank body into a gas distribution chamber, a decontamination chamber, a catalytic hydrolysis chamber and a gas collecting chamber. The gas inlet is arranged on the gas distribution chamber side of the reaction tank body, and the gas outlet is arranged on the gas collecting chamber side of the reaction tank body. A decontamination agent is placed in the decontamination chamber, and an organic catalytic converter is placed in the catalytic hydrolysis chamber. The gas enters the gas distribution chamber from the gas inlet, flows uniformly toward the decontamination chamber and the catalytic hydrolysis chamber, and is discharged through the outlet after merging in the gas collecting chamber. The gas has low resistance along the gas flow and low pressure loss, and can meet the requirements of removing harmful impurities in blast furnace flue gas and catalytically hydrolyzing organic sulfides.

The invention relates to the technical field of tannase, in particular to a recombinant strain for preparing gallic acid through an enzyme method and a construction method of the recombinant strain, the amino acid sequence of tannase is SEQ ID NO.1, tannase of Aspergillus niger CBS 513.88 is selected, and the nucleotide sequence of the tannase is SEQ ID NO.2. According to the invention, tannase genes in aspergillus niger are optimized, the performance of catalytic hydrolysis of tara tannin is improved, and a gene integrated recombinant aspergillus niger strain is constructed and is applied to production of tannase.

The present invention provides a catalyst composition having excellent properties for converting sulfur, sulfur compounds, and carbon monoxide contained in a gas stream by catalytic hydrolysis, hydrogenation, and water gas shift reactions. The catalyst comprises a base layer of molybdenum and cobalt and an overlay of molybdenum and cobalt. These metals are present in the catalyst in a certain concentration range and relative weight ratio. The base layer metals are present in the catalyst in a specified range relative to the overlay and total metals. The base layer metals are formed by co-grinding an inorganic oxide with the catalytically active metals molybdenum and cobalt. The co-ground mixture is calcined and then impregnated with overlay molybdenum and overlay cobalt.

The invention belongs to the technical field of electrocatalytic hydrolysis, and particularly relates to a preparation method and application of high-activity NiCe (OH) n / NF. A noble metal catalyst is not used, so that the use cost is greatly reduced. The positive electrode obtains glucaric acid in an electrooxidation mode, meanwhile, the active center NiOOH can stably exist in an alkaline environment in a circulation mode, and the negative electrode produces hydrogen. And due to the redox cycle between Ni (OH) 2 and NiOOH, the overpotential can be effectively reduced, and the electric energy loss is reduced. And due to the synergistic effect of a small amount of cerium, the system can realize high-efficiency hydrogen production while obtaining the glucaric acid with high additional value. According to the method, glucose is catalyzed by using a circulating system to obtain glucaric acid, meanwhile, the anode glucose yield reaches 70%, and the cathode hydrogen evolution efficiency is close to 100%. Compared with a traditional water electrolysis hydrogen production process, the glucose oxidation assisted low-potential couple circulation coupling hydrogen production method conforms to'green chemistry 'and is lower in cost, and harsh reaction conditions and complex separation steps are avoided.

The invention discloses a high-stability hydrolysis catalyst for removing carbonyl sulfide in blast furnace gas at a low temperature as well as a preparation method and application of the high-stability hydrolysis catalyst, and belongs to the technical field of carbonyl sulfide removal. The preparation method comprises the following steps: preparing MgO nanoparticles; the preparation method comprises the following steps: dissolving Nd (NO3) 3.6 H2O and Al (NO3) 3.9 H2O in deionized water, uniformly stirring at normal temperature until the Nd (NO3) 3.6 H2O and the Al (NO3) 3.9 H2O are completely dissolved, then adding MgO nanoparticles into the mixed solution, ultrasonically stirring, oscillating and dehydrating the obtained mixture to obtain white powder, drying and roasting to obtain the product. The preparation method is simple, the prepared catalyst has a large specific surface area, many alkaline active sites and good photocatalytic characteristics, compared with an existing COS removal technology and an existing photo-thermal concerted catalytic hydrolysis technology, COS can be efficiently removed at a low temperature, reaction energy consumption is reduced, and the method is suitable for industrial production. The method has a wide application prospect in efficient removal of COS.