Rhodococcus erythropolis for biological desulfurization and its use

A technology of Rhodococcus erythropolis and biological desulfurization, applied in the direction of bacteria, etc., can solve the problems of long growth cycle of strains, shortening the life of biocatalysts, and insufficient catalytic activity of desulfurization strains, and achieve good oil resistance, short growth cycle, and desulfurization highly active effect

Active Publication Date: 2006-11-01
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although many strains have been isolated that can be used in the fuel oil BDS process, there are still many limiting factors that hinder the commercial application of the petroleum BDS process
These limiting factors are, for example: the growth period of the strain used for desulfurization is too long, the specific catalytic activity of the desulfurization strain is not high enough, the sulfate produced in the desulfurization process seriously inhibits the activity of the biocatalyst, and the toxicity of petroleum to the desulfurization strain shortens biocatalyst lifetime, etc.

Method used

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  • Rhodococcus erythropolis for biological desulfurization and its use
  • Rhodococcus erythropolis for biological desulfurization and its use
  • Rhodococcus erythropolis for biological desulfurization and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: the screening of FSD-2 bacterial strain

[0047] Oil-contaminated soil samples were taken from the vicinity of the Gudao Oilfield in Shandong, and a three-step process was used to screen, isolate, and purify microorganisms that selectively desulfurize oil. In the first step, add 0.5 g of oil-contaminated soil sample to 50 ml of sulfur-free basic medium FBM containing a final concentration of 1 mmol / l DBT (FBM medium contains 10.0 g / l glucose, 2.0 g / l NH 4 Cl, 6.3g / l KH 2 PO 4 , 8.0g / l K 2 HPO 4 , 0.2g / lMgCl 2 ·6H 2 O, etc., the FBM medium containing 1mmol / l DBT is FBMD medium), 30 DEG C shaking culture 5 days, transfer to FBMD medium with 10% inoculum size, cultivate 2 days under the same condition, transfer 2 times repeatedly -3 times. In the second step, after such 2-3 enrichment cultures, take 1ml of the culture, centrifuge at 3000rpm for 5min, and take 0.5ml of the supernatant for Gibb's analysis (after DBT is degraded by the "4S" pathway, the g...

Embodiment 2

[0049] Example 2: FSD-2 strain metabolite analysis

[0050] Pick the FSD-2 strain cultured on the FMAD agar slant and inoculate into 50ml FMAD liquid medium. Components of FMAD liquid medium: 5g / l glucose, 2g / l NH 4 Cl, 14.04g / l Na 2 HPO 4 , 2.24g / l KH 2 PO 4 , 0.2g / l MgCl 2 ·6H 2 O, the addition amount of trace element solution mother solution and vitamin solution mother solution is 1.0ml / l respectively, the final concentration of DBT is 1.0mmol / l, and the pH value is 7.0~7.2. Trace element mother liquor contains 0.5g / l FeCl 2 4H 2 O, 0.5g / l ZnCl 2 , 0.5g / l MnCl 2 4H 2 O, 0.1g / l NaMoO 4 2H 2 O, 0.05g / l CuCl 2 , 0.05g / l NaWO 4 2H 2 O and 120mmol / l HCl. Vitamin mother solution contains 400mg / l calcium pantothenate, 200mg / l inositol, 400mg / l niacin, 400mg / l pyridoxine hydrochloride, 200mg / l p-aminotoluene and 0.5mg / l VB12. Culture at 30℃, 150 rpm After 24-48 hours, take 10ml of the culture, add 2ml of dichloromethane to extract the metabolites in the culture, a...

Embodiment 3

[0051] Embodiment 3: FSD-2 bacterial strain growth cell degrades DBT

[0052] Pick the FSD-2 strain cultured on the FMAD agar slant and inoculate into 50ml FMAD liquid medium. Components of FMAD liquid medium: 5g / l glucose, 2g / l NH 4 Cl, 14.04g / l Na 2 HPO 4 , 2.24g / l KH 2 PO 4 , 00.2g / l MgCl 2 ·6H 2 O, the addition amount of trace element solution mother solution and vitamin solution mother solution is 1.0ml / l respectively, the final concentration of DBT is 0.5mmol / l, and the pH value is 7.0~7.2. Trace element mother liquor contains 0.5g / l FeCl 2 4H 2 O, 0.5g / l ZnCl 2 , 0.5g / l MnCl 2 4H 2 O, 0.1g / l NaMoO 4 2H 2 O, 0.05g / l CuCl 2 , 0.05g / l NaWO 4 2H 2 O and 120mmol / l HCl. Vitamin mother solution contains 400mg / l calcium pantothenate, 200mg / l inositol, 400mg / l niacin, 400mg / l pyridoxine hydrochloride, 200mg / l p-aminotoluene and 0.5mg / l VB12. Culture at 30℃, 150rpm , regularly sampled for Gibb's analysis. Gibb's analysis method is as follows: centrifuge the cul...

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Abstract

This invention involves Rhodococcus erythropolis FSD-2 used to biologic desulphurization and the application of it. The strain of Rhodococcus erythropolis FSD-2 is held by 2004-10-11 in CGMCC and the holding number is CGMCC No.1209. it is to make the soil that is polluted by petroleum and got from Shandong Gu Dao oil field as the raw materials in order to getting the Rhodococcus erythropolis FSD-2, besides, for getting the strain it is to make DBT as selection pressure for screen separating. The characteristics of the Rhodococcus erythropolis FSD-2 are short growth periodicity, high activity of desulphurization, strong tolerance of sulfate ion, good oil resistivity, high stability catalysis and the ability of specifically fracturing the C-S bond in organosulfur compounds. The invention also involves a method of biologic desulphurization that is to make the Rhodococcus erythropolis FSD-2 or the extraction of its cells as the biocatalyst and used for the inorganic fuel that contains organosulfur compounds.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a newly screened bacterial strain Rhodococcus erythropolis. The Rhodococcus erythropolis can selectively break the C-S bond of the carbon-containing substance, and keep the calorific value of the carbon-containing substance basically unchanged. The Rhodococcus erythropolis of the present invention is particularly suitable for desulfurization in substances containing organic sulfur compounds, such as for desulfurization in sulfur-containing coal or crude oil and its fractions. Background technique [0002] Sulfur is the third most abundant element in petroleum after carbon and hydrogen, and it is an undesirable component in both processed and unprocessed fuels. Sulfur in petroleum causes corrosion of equipment during the petroleum refining process. In addition, SO produced by combustion of sulfur-containing fuel oil 2 Will cause environmental pollution. Environmental authorities have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12S1/02
Inventor 张全唐似茵佟明友黎元生高会杰
Owner CHINA PETROLEUM & CHEM CORP
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