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Solid microbial agent to remedy soil contaminated by petroleum, preparation method thereof and application

A technology for oil pollution and soil remediation, applied in the restoration of polluted soil, biochemical equipment and methods, methods based on microorganisms, etc., to achieve low cost, relieve incompatibility, and stimulate growth

Inactive Publication Date: 2009-12-09
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new type of material called oily residue remover (OR) or hydrocarbon contamination treatment solid bacillus that helps remove harmful substances from an area containing excessive amounts of water without causing any negative side effect on nearby areas. It uses native bacterium like Orosporum ovatus for its beneficial properties such as reducing chlorophyll production, improving nutrients absorption efficiency, promoting decomposition of certain types of chemical waste products, increasing carbon footprint value, enhancing biodegradability, etc., while being easier to use at home compared to current methods involving expensive equipment.

Problems solved by technology

This patented technical problem addressed by this patents relates to environmental cleanup techniques involving removing harmful substances like pesticides and other residues found on surfaces due to industrial activities including land use operations and waste disposals. Existing technologies involve expensive processes, often requiring long periods of time before they work effectively at full scale. There also exist concerns over potential health hazards associated with certain materials present within soil samples containing significant amounts of hydrocarbon gases released through decomposition reactions between natural sources and manmade environments. Therefore, new solutions aim towards effective removal of unwanted components without causing damage to surrounding areas should improve public awarenement regarding the issue related to environmental protection issues.

Method used

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  • Solid microbial agent to remedy soil contaminated by petroleum, preparation method thereof and application

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1: the preparation of oil-contaminated soil remediation bacterial agent

[0028] The bacteria used are the aforementioned Acinetobacter radiata and Rhodococcus erythropolis, which are cultured on a slant, prepared seed liquid, liquid-state fermentation culture, and solid-state fermentation culture to obtain the repair bacteria agent. The specific operation steps are:

[0029] (1) Slant culture: Prepare beef extract-peptone slant medium by conventional methods. The specific steps are: 3g beef extract, 5g peptone, 5g NaCl, 1000mL distilled water, pH7.2-7.4, 1.5%-2% agar, extinguished at 121°C Bacteria 20 ~ 30min. Make a slant after sterilization, transfer the preserved strain to the test tube slant, and cultivate it at 25-30°C for 16 hours before use;

[0030](2) Preparation of primary seed liquid: the liquid medium is still beef extract peptone medium, and the prepared liquid medium is divided into 150 or 250 mL Erlenmeyer flasks with a liquid volume of 50 mL...

Embodiment 2

[0034] Embodiment 2: the preparation of oil-contaminated soil remediation bacterial agent

[0035] The materials and parameters used in Example 1 are basically the same, except that in the liquid fermentation culture: the inoculation amount of the primary seed liquid is 10% of the volume of the liquid fermentation medium, and after inoculation, it is placed on a shaking table and cultivated for 32h at a temperature of 30°C. ℃, the shaker speed is 250rpm, the effective number of viable bacteria of Acinetobacter radioresistant and Rhodococcus erythropolis is about 5.0×10 8 CFU / mL and 2.5×10 8 CFU / mL; in solid-state fermentation culture: the culture medium is a mixture of high-temperature sterilized bran and peat with a weight ratio of 2:1, the water content of the medium is adjusted to 60%, the inoculation amount of the fermentation broth is 10%, and the culture time is 4 day, in the finished product of the solid microbial agent for remediation of oil-contaminated soil, the eff...

Embodiment 3

[0036] Embodiment 3: the preparation of oil-contaminated soil remediation bacterial agent

[0037] The materials and parameters used in Example 1 or 2 are basically the same, but the difference is that in the liquid fermentation culture: the inoculation amount of the primary seed liquid is 7% of the volume of the liquid fermentation medium, and after inoculation, it is placed on a shaking table and cultivated for 30h. at 28°C, with a shaker rotation speed of 200rpm, the effective viable counts of Acinetobacter radioresistant and Rhodococcus erythropolis were about 4.5×10 8 CFU / mL and 2.0×10 8 CFU / mL; in solid-state fermentation culture: the culture medium is a mixture of bran and peat with a weight ratio of 1:1 after high-temperature sterilization, the water content of the medium is adjusted to 45%, the inoculation amount of the fermentation broth is 8%, and the culture time is 3 day, in the finished product of the solid microbial agent for remediation of oil-contaminated soi...

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Abstract

The invention discloses a solid microbial agent used for remedying the soil contaminated by petroleum, belonging to the technical field of bioremediation of soil contaminants. First-class seed liquid of acinetobacter radioresistens and rhodococcus erythropolis are mixed in equal parts after two steps of fermentation in liquid and solid states and cultivation to obtain the solid microbial agent, wherein, the number of effective viable bacteria of acinetobacter radioresistens and rhodococcus erythropolis is 10 to 10 CFU/g. The solid microbial agent in the invention has the advantages of low cost, high number of viable bacteria; the invention further has the advantages that micro-ecological environment improvement of the soil can be improved and secondary contamination cannot be caused to the environment, thus applicable to the bioremediation of the soil which is contaminated by petroleum and petroleum products.

Description

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Claims

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Application Information

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Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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